Status
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Gene information
General information
TC003373 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 2959252..2961630
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003373
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_07947

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: number 2nd lateral oviduct: - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003373 RA
      MSANNVNAQ PAPTTQATA TIKSRPNIM PSSYLYTSS 
      SALLNRNAE NKPTPPPTL PKYTSSFNA GSGLNRDRD 
      KDSIGGSYR LSSLDRLAM RQKFMDQQS PTNGTITTQ 
      TNGSEATSI QTKREMFFT STTDTKDSN RVKPPEPPA 
      KTPPEQPAK EDSKESTPE IVKERPKLK ELDGYVGFA 
      NLPNQVYRK AVKKGFEFT LMVVGESGL GKSTLINSM 
      FLTEIYNAT DYPGPTQRL KKTVAVETT RVMLKENGV 
      NLLLTMVDT PGFGDAVDN SNCWAPIIE YIESKYEDY 
      LNSEARVTR KQIPDQRVH CCLYFIQPS GHGLKSLDI 
      EFMKRLCDK VNIIPIIAK ADTLTSDEC ALFKKQILN 
      EIAQNKIKI YEFPDTSED DEEHKLNKS LKERVPFAV 
      VGSNTVIEV DGKKVRGRK YPWGIAEVE NLEHCDFIA 
      LRNMIIRTH LQDLKDVTN NVHYENYRC RKLAGLGVD 
      GKPSRISNK NPLAQMDEE KREHDLKMK KMEAEMEQV 
      FEMKVREKK QKLKDSEAE LTRRHEATK KQLEQQAKE 
      LEEKRRQFE MEKEQWEKE NQITVEELR RRSLEGSRE 
      AVDGKKEKK KKGLF
    CDS sequence
    • >TC003373 RA
      atgagtgcc aacaatgtg aatgctcaa cctgcccct 
      accacccaa gccaccgcc accatcaag tccagaccc 
      aacatcatg cccagcagc tacttgtac acttctagc 
      agtgccctt ctcaaccgc aacgctgag aacaaaccc 
      acgccgccg cccaccctc cccaagtac acctccagc 
      ttcaatgcc gggagcggt ctcaaccgg gacagggac 
      aaggacagc atcgggggt tcctaccgc ctctcaagc 
      ctggaccga ctagccatg aggcagaaa ttcatggac 
      cagcaaagc cccacaaat ggcaccatc accacccaa 
      acaaatggc agtgaagcc acttcaatc caaaccaaa 
      cgagagatg ttcttcact tcaaccacc gacacgaag 
      gactccaac agggttaaa ccgccagaa cccccggcc 
      aagacaccc ccagaacaa ccggccaaa gaagacagc 
      aaggaatcc acccctgag attgtcaag gaacggcct 
      aaactcaaa gaactggac ggttacgtc ggctttgcc 
      aatctaccg aaccaggtg taccgaaag gccgtgaag 
      aagggcttc gagttcacc ctcatggtg gtgggggag 
      tcaggcttg ggcaaatcc accctaatc aactcgatg 
      tttctaaca gaaatctac aacgccacc gactaccct 
      gggcccacc cagcgcctc aagaagaca gtcgccgtg 
      gagaccacc cgagtcatg ctcaaggaa aacggcgta 
      aacctcctc ctcaccatg gtggacact cccggcttc 
      ggcgacgcc gtcgacaac agcaactgc tgggccccc 
      atcatcgaa tacatcgag tccaaatac gaggactac 
      ctgaactct gaggcccgc gtcacccgg aaacaaatc 
      cccgaccag cgggtccac tgctgcttg tacttcatc 
      caaccgtcg ggacacggc ttaaaatcc ctcgatata 
      gagtttatg aagcgtttg tgtgataaa gtgaacatt 
      attcccata atagcaaag gccgacacg ctcacatcc 
      gacgaatgc gccctcttc aagaagcaa atcctgaac 
      gagatcgcc cagaacaag atcaaaatc tacgagttc 
      cccgacacg tccgaagac gacgaggag cacaagctg 
      aacaagtcg ctgaaggag cgcgtgccg ttcgctgtg 
      gtgggctcg aacacggtc atagaagtg gacgggaag 
      aaggtgcgc ggccggaag tacccctgg gggatcgcc 
      gaagtcgag aacttggaa cactgtgat tttatcgca 
      cttcgcaac atgatcata cgcacacat ctccaggac 
      ttgaaggac gtcaccaat aatgtgcac tacgagaat 
      taccgttgc cgcaagttg gccggttta ggggtggat 
      gggaagccg tcccggatt tcgaacaaa aatccactg 
      gcgcagatg gacgaggag aagcgcgag cacgatctc 
      aagatgaag aagatggag gcggagatg gagcaagtg 
      tttgaaatg aaggttcgt gagaagaag caaaagctg 
      aaggatagt gaggcggaa ctgacgagg agacacgag 
      gcgacgaag aagcagctg gagcagcaa gccaaggag 
      ttggaggag aagaggagg cagttcgag atggagaag 
      gagcagtgg gagaaggag aaccaaatc acggtggag 
      gagctgcgt cggaggtcg ctcgaaggg agcagggag 
      gccgtcgat ggcaagaag gagaagaag aaaaaggga 
      cttttctaa
    mRNA sequence
    • >TC003373 RA
      taatagtga taaaacggt ggaaagttg tacgttatc 
      ggtggagcg gaagagacg ccacgggcg tggctgacc 
      aagcaaaca ttactcata cccggcatc caactgtca 
      atttgacat cacaaatcg cgaagtgaa taacaatgg 
      cagtgtagg tccactagg tgttttacc acaatgagt 
      gccaacaat gtgaatgct caacctgcc cctaccacc 
      caagccacc gccaccatc aagtccaga cccaacatc 
      atgcccagc agctacttg tacacttct agcagtgcc 
      cttctcaac cgcaacgct gagaacaaa cccacgccg 
      ccgcccacc ctccccaag tacacctcc agcttcaat 
      gccgggagc ggtctcaac cgggacagg gacaaggac 
      agcatcggg ggttcctac cgcctctca agcctggac 
      cgactagcc atgaggcag aaattcatg gaccagcaa 
      agccccaca aatggcacc atcaccacc caaacaaat 
      ggcagtgaa gccacttca atccaaacc aaacgagag 
      atgttcttc acttcaacc accgacacg aaggactcc 
      aacagggtt aaaccgcca gaacccccg gccaagaca 
      cccccagaa caaccggcc aaagaagac agcaaggaa 
      tccacccct gagattgtc aaggaacgg cctaaactc 
      aaagaactg gacggttac gtcggcttt gccaatcta 
      ccgaaccag gtgtaccga aaggccgtg aagaagggc 
      ttcgagttc accctcatg gtggtgggg gagtcaggc 
      ttgggcaaa tccacccta atcaactcg atgtttcta 
      acagaaatc tacaacgcc accgactac cctgggccc 
      acccagcgc ctcaagaag acagtcgcc gtggagacc 
      acccgagtc atgctcaag gaaaacggc gtaaacctc 
      ctcctcacc atggtggac actcccggc ttcggcgac 
      gccgtcgac aacagcaac tgctgggcc cccatcatc 
      gaatacatc gagtccaaa tacgaggac tacctgaac 
      tctgaggcc cgcgtcacc cggaaacaa atccccgac 
      cagcgggtc cactgctgc ttgtacttc atccaaccg 
      tcgggacac ggcttaaaa tccctcgat atagagttt 
      atgaagcgt ttgtgtgat aaagtgaac attattccc 
      ataatagca aaggccgac acgctcaca tccgacgaa 
      tgcgccctc ttcaagaag caaatcctg aacgagatc 
      gcccagaac aagatcaaa atctacgag ttccccgac 
      acgtccgaa gacgacgag gagcacaag ctgaacaag 
      tcgctgaag gagcgcgtg ccgttcgct gtggtgggc 
      tcgaacacg gtcatagaa gtggacggg aagaaggtg 
      cgcggccgg aagtacccc tgggggatc gccgaagtc 
      gagaacttg gaacactgt gattttatc gcacttcgc 
      aacatgatc atacgcaca catctccag gacttgaag 
      gacgtcacc aataatgtg cactacgag aattaccgt 
      tgccgcaag ttggccggt ttaggggtg gatgggaag 
      ccgtcccgg atttcgaac aaaaatcca ctggcgcag 
      atggacgag gagaagcgc gagcacgat ctcaagatg 
      aagaagatg gaggcggag atggagcaa gtgtttgaa 
      atgaaggtt cgtgagaag aagcaaaag ctgaaggat 
      agtgaggcg gaactgacg aggagacac gaggcgacg 
      aagaagcag ctggagcag caagccaag gagttggag 
      gagaagagg aggcagttc gagatggag aaggagcag 
      tgggagaag gagaaccaa atcacggtg gaggagctg 
      cgtcggagg tcgctcgaa gggagcagg gaggccgtc 
      gatggcaag aaggagaag aagaaaaag ggacttttc 
      taacactga atgtatagt tttggtgtg gtaccttct 
      taacttcat ggtaatttc tttaattaa gtgacttta 
      acggcagag atagtttcg tctagtgag actcccgca 
      atagattga gaaattatg ttttcaaat acacaactg 
      ccattaaaa tagcgtaat tgtctacgt gccgattta 
      ctatttttt accatgatt acttgtgag ctgtttaga 
      tcgtacatt aagagataa tgaatggga attatttaa 
      cttatataa ataaaatca attatttga acagaagtg 
      tcttttcgt gacatcttg cttggaagc aatgtgcca 
      ttatttttt tttattttt atttacttg tattaactg 
      taaaaataa attcttttc tgacag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY