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Gene information
General information
TC003370 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 2798166..2801220
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.

    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.

    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_07945

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Left: Right:
    Protein sequence
    • >TC003370 RA
    CDS sequence
    • >TC003370 RA
      atgccgaga cgtgctaac aacagttct tctgaggat 
      gatcccccc aaaaagaag acttgcagc aactccttt 
      gatttaaga catggggag tatctaaaa gacatcacg 
      gggaagcag tggaaattg gggaaacct gtgggtgtt 
      gggggcttt ggggagatc tacctggcc tctgatgtc 
      ctcaccaaa gaggtcaag actgattgt cagtacgtg 
      gctaaggtg gaaagccac aaaaacggg ccgcttttt 
      gtcgaagtc aattgttac ctgaggatt gctaaacgc 
      gaaatgatt gaagagtgg aagcgagca aagaatata 
      agttccctc ggaatgccc cactatgtg gcgtcaggt 
      tcccacgtc cacaagggt gaaaagtat cgtttctta 
      atcctcccg aaatttgac agagacttg gaaaaagtc 
      ctccaagag aagaaaatc ctaaatctt aaaacagtg 
      ctcacaata tccctccaa ataattgat gttttggaa 
      tacatccac agtaaaggc tatatgcac tccgatatc 
      aaggcatct aatattctt ctaagtaac caaaataag 
      cccaaacca accgtctgt aaacgagtg tcaagcggc 
      tgcacacga gtatcaagc ggctgcacg ttcaccagt 
      cgtctccgt gcatgtcgc aaaaaacca accagaatc 
      actagaaac ttaaggccg aacgctaaa gtcgccttt 
      tatctcgac gacatacca aattttaat caaatcttc 
      actaattgt gtgtatgaa aaaccaaca acaacacag 
      gaccagatt tatctcctt gattacggt ctggcgtcc 
      aaatttcta acatcaaac ggcgaacat aaaccgttt 
      tgcgttgac gaacgtaaa gctcacgct gggactgtt 
      ttattttgt tcgcgtgat gctcacaag ggggtgcat 
      tcgcgacgc tcagatttg gagtctttg gggtacaat 
      attatttat tggctgact gggactctg ccatgggct 
      ggtgatgca gacaacccg gaaatcgta gccaagaag 
      aagcagaga gctatggct gatgtggag tattttatg 
      aactattgt ttcagtgaa tatcctaag tttctggaa 
      gaatatttt aagtatatt cagaaactg gaatttcag 
      gaaaagcct gactacagc tactgcaag aagctcttt 
      agggagagt ttgaaaacg aacggttac aaagacgat 
      tcgcgcttt gactttgac aatgtgcaa agaaacaaa 
      ttattgaag ccgactaga atcaggaag agaactaag 
      gaaaatatc agggcacgg aaatttatc cacacaata 
      tcacgtcta cctctacaa tcaaacatc cccattaaa 
      ccgctactg agaaagaaa gtgaaaaat aagaaagta 
      aaacctaag gcaaattgg tttggggag ctcactgac 
      cctgagata atactgaag caagctcgt aagaatagc 
      gaaaatact gacgcaaac ttagctagt aacgttgcg 
      gaattagat ataaatgcc ttgaaccca acgtacgct 
      atgattgaa gtttataat aaatgtagg gagaggata 
      gacgcatca cccacttac aagagtgac agttgtata 
      gagaccgtg gatggttac acatctgcg atgatgtct 
      gtgttagag aaaatgaaa aagaggaga gagcttgag 
      ttggaagaa atggctgtg aatctcagt aatacaagg 
      agtggcaga gttcagaaa agaattcgt aaacccaat 
      aggaaatat gaatctcca gactggtcg cctcctcag 
      acacgaacc tcgaaacca gtgataaag aaggatcaa 
      gcacgtaag gtaaaaagt ggggttttg aggaggact 
      tacagttta agaggttaa
    mRNA sequence
    • >TC003370 RA
      aggcgtttc aattgagcg aaacgtgaa gatggccgt 
      gaaaattgt cagtgacgt ttctttcac cttttgatt 
      tttggcaaa ataacagtt tttaccact tttctaact 
      tgttgtatg aaaagtaag tattgtttc ttattgttt 
      acgaccggg acgtgccac ttgaccttg ctgttgtcg 
      ttatgtaaa tgataaggt tattgggga aatgtcgtt 
      agattttga aatcttgtt gttatttac ataaaaaaa 
      aaacgtttc ttcttgaag ggtcctgtg tctgggtaa 
      tcacatgaa ttaggcagg attgtttat agacttcac 
      actttcgtt aaaataatg ccgagacgt gctaacaac 
      agttcttct gaggatgat ccccccaaa aagaagact 
      tgcagcaac tcctttgat ttaagacat ggggagtat 
      ctaaaagac atcacgggg aagcagtgg aaattgggg 
      aaacctgtg ggtgttggg ggctttggg gagatctac 
      ctggcctct gatgtcctc accaaagag gtcaagact 
      gattgtcag tacgtggct aaggtggaa agccacaaa 
      aacgggccg ctttttgtc gaagtcaat tgttacctg 
      aggattgct aaacgcgaa atgattgaa gagtggaag 
      cgagcaaag aatataagt tccctcgga atgccccac 
      tatgtggcg tcaggttcc cacgtccac aagggtgaa 
      aagtatcgt ttcttaatc ctcccgaaa tttgacaga 
      gacttggaa aaagtcctc caagagaag aaaatccta 
      aatcttaaa acagtgctc acaatatcc ctccaaata 
      attgatgtt ttggaatac atccacagt aaaggctat 
      atgcactcc gatatcaag gcatctaat attcttcta 
      agtaaccaa aataagccc aaaccaacc gtctgtaaa 
      cgagtgtca agcggctgc acacgagta tcaagcggc 
      tgcacgttc accagtcgt ctccgtgca tgtcgcaaa 
      aaaccaacc agaatcact agaaactta aggccgaac 
      gctaaagtc gccttttat ctcgacgac ataccaaat 
      tttaatcaa atcttcact aattgtgtg tatgaaaaa 
      ccaacaaca acacaggac cagatttat ctccttgat 
      tacggtctg gcgtccaaa tttctaaca tcaaacggc 
      gaacataaa ccgttttgc gttgacgaa cgtaaagct 
      cacgctggg actgtttta ttttgttcg cgtgatgct 
      cacaagggg gtgcattcg cgacgctca gatttggag 
      tctttgggg tacaatatt atttattgg ctgactggg 
      actctgcca tgggctggt gatgcagac aacccggaa 
      atcgtagcc aagaagaag cagagagct atggctgat 
      gtggagtat tttatgaac tattgtttc agtgaatat 
      cctaagttt ctggaagaa tattttaag tatattcag 
      aaactggaa tttcaggaa aagcctgac tacagctac 
      tgcaagaag ctctttagg gagagtttg aaaacgaac 
      ggttacaaa gacgattcg cgctttgac tttgacaat 
      gtgcaaaga aacaaatta ttgaagccg actagaatc 
      aggaagaga actaaggaa aatatcagg gcacggaaa 
      tttatccac acaatatca cgtctacct ctacaatca 
      aacatcccc attaaaccg ctactgaga aagaaagtg 
      aaaaataag aaagtaaaa cctaaggca aattggttt 
      ggggagctc actgaccct gagataata ctgaagcaa 
      gctcgtaag aatagcgaa aatactgac gcaaactta 
      gctagtaac gttgcggaa ttagatata aatgccttg 
      aacccaacg tacgctatg attgaagtt tataataaa 
      tgtagggag aggatagac gcatcaccc acttacaag 
      agtgacagt tgtatagag accgtggat ggttacaca 
      tctgcgatg atgtctgtg ttagagaaa atgaaaaag 
      aggagagag cttgagttg gaagaaatg gctgtgaat 
      ctcagtaat acaaggagt ggcagagtt cagaaaaga 
      attcgtaaa cccaatagg aaatatgaa tctccagac 
      tggtcgcct cctcagaca cgaacctcg aaaccagtg 
      ataaagaag gatcaagca cgtaaggta aaaagtggg 
      gttttgagg aggacttac agtttaaga ggttaagtt 
      taactactc gtttttacg ttcgtgtat taactatta 
      agactgcaa aatttgtga ctagaatac tgagttgtt 
      tgtttcaaa aaatgtgca gtagatttg attgtaaat 
      gaatagttt gttgattgt aataaataa acagttggc 
      gctccaatc aattatcaa ctgaaatta tttctttaa 
      ttgtgctcc atataagta cattagtat gattcgctt 
      gtaccgcgc caattgttt cttaagtct gaagttata 
      gttaattcg gcagttgtg tttgttctt gatttatat 
      tatcgtttt aattcatct cagcgcaaa atagtgtaa 
      aattcttgt ggtaaattt actgtgatt ttgacttat 
      tgtgaaata aaacaat

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY