| General information | |||
| TC003370 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 2798166..2801220 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC003370 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_07945 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC003370 RA
MPRRANNSS SEDDPPKKK TCSNSFDLR HGEYLKDIT GKQWKLGKP VGVGGFGEI YLASDVLTK EVKTDCQYV AKVESHKNG PLFVEVNCY LRIAKREMI EEWKRAKNI SSLGMPHYV ASGSHVHKG EKYRFLILP KFDRDLEKV LQEKKILNL KTVLTISLQ IIDVLEYIH SKGYMHSDI KASNILLSN QNKPKPTVC KRVSSGCTR VSSGCTFTS RLRACRKKP TRITRNLRP NAKVAFYLD DIPNFNQIF TNCVYEKPT TTQDQIYLL DYGLASKFL TSNGEHKPF CVDERKAHA GTVLFCSRD AHKGVHSRR SDLESLGYN IIYWLTGTL PWAGDADNP EIVAKKKQR AMADVEYFM NYCFSEYPK FLEEYFKYI QKLEFQEKP DYSYCKKLF RESLKTNGY KDDSRFDFD NVQRNKLLK PTRIRKRTK ENIRARKFI HTISRLPLQ SNIPIKPLL RKKVKNKKV KPKANWFGE LTDPEIILK QARKNSENT DANLASNVA ELDINALNP TYAMIEVYN KCRERIDAS PTYKSDSCI ETVDGYTSA MMSVLEKMK KRRELELEE MAVNLSNTR SGRVQKRIR KPNRKYESP DWSPPQTRT SKPVIKKDQ ARKVKSGVL RRTYSLRG
- >TC003370 RA
atgccgaga cgtgctaac aacagttct tctgaggat gatcccccc aaaaagaag acttgcagc aactccttt gatttaaga catggggag tatctaaaa gacatcacg gggaagcag tggaaattg gggaaacct gtgggtgtt gggggcttt ggggagatc tacctggcc tctgatgtc ctcaccaaa gaggtcaag actgattgt cagtacgtg gctaaggtg gaaagccac aaaaacggg ccgcttttt gtcgaagtc aattgttac ctgaggatt gctaaacgc gaaatgatt gaagagtgg aagcgagca aagaatata agttccctc ggaatgccc cactatgtg gcgtcaggt tcccacgtc cacaagggt gaaaagtat cgtttctta atcctcccg aaatttgac agagacttg gaaaaagtc ctccaagag aagaaaatc ctaaatctt aaaacagtg ctcacaata tccctccaa ataattgat gttttggaa tacatccac agtaaaggc tatatgcac tccgatatc aaggcatct aatattctt ctaagtaac caaaataag cccaaacca accgtctgt aaacgagtg tcaagcggc tgcacacga gtatcaagc ggctgcacg ttcaccagt cgtctccgt gcatgtcgc aaaaaacca accagaatc actagaaac ttaaggccg aacgctaaa gtcgccttt tatctcgac gacatacca aattttaat caaatcttc actaattgt gtgtatgaa aaaccaaca acaacacag gaccagatt tatctcctt gattacggt ctggcgtcc aaatttcta acatcaaac ggcgaacat aaaccgttt tgcgttgac gaacgtaaa gctcacgct gggactgtt ttattttgt tcgcgtgat gctcacaag ggggtgcat tcgcgacgc tcagatttg gagtctttg gggtacaat attatttat tggctgact gggactctg ccatgggct ggtgatgca gacaacccg gaaatcgta gccaagaag aagcagaga gctatggct gatgtggag tattttatg aactattgt ttcagtgaa tatcctaag tttctggaa gaatatttt aagtatatt cagaaactg gaatttcag gaaaagcct gactacagc tactgcaag aagctcttt agggagagt ttgaaaacg aacggttac aaagacgat tcgcgcttt gactttgac aatgtgcaa agaaacaaa ttattgaag ccgactaga atcaggaag agaactaag gaaaatatc agggcacgg aaatttatc cacacaata tcacgtcta cctctacaa tcaaacatc cccattaaa ccgctactg agaaagaaa gtgaaaaat aagaaagta aaacctaag gcaaattgg tttggggag ctcactgac cctgagata atactgaag caagctcgt aagaatagc gaaaatact gacgcaaac ttagctagt aacgttgcg gaattagat ataaatgcc ttgaaccca acgtacgct atgattgaa gtttataat aaatgtagg gagaggata gacgcatca cccacttac aagagtgac agttgtata gagaccgtg gatggttac acatctgcg atgatgtct gtgttagag aaaatgaaa aagaggaga gagcttgag ttggaagaa atggctgtg aatctcagt aatacaagg agtggcaga gttcagaaa agaattcgt aaacccaat aggaaatat gaatctcca gactggtcg cctcctcag acacgaacc tcgaaacca gtgataaag aaggatcaa gcacgtaag gtaaaaagt ggggttttg aggaggact tacagttta agaggttaa
- >TC003370 RA
aggcgtttc aattgagcg aaacgtgaa gatggccgt gaaaattgt cagtgacgt ttctttcac cttttgatt tttggcaaa ataacagtt tttaccact tttctaact tgttgtatg aaaagtaag tattgtttc ttattgttt acgaccggg acgtgccac ttgaccttg ctgttgtcg ttatgtaaa tgataaggt tattgggga aatgtcgtt agattttga aatcttgtt gttatttac ataaaaaaa aaacgtttc ttcttgaag ggtcctgtg tctgggtaa tcacatgaa ttaggcagg attgtttat agacttcac actttcgtt aaaataatg ccgagacgt gctaacaac agttcttct gaggatgat ccccccaaa aagaagact tgcagcaac tcctttgat ttaagacat ggggagtat ctaaaagac atcacgggg aagcagtgg aaattgggg aaacctgtg ggtgttggg ggctttggg gagatctac ctggcctct gatgtcctc accaaagag gtcaagact gattgtcag tacgtggct aaggtggaa agccacaaa aacgggccg ctttttgtc gaagtcaat tgttacctg aggattgct aaacgcgaa atgattgaa gagtggaag cgagcaaag aatataagt tccctcgga atgccccac tatgtggcg tcaggttcc cacgtccac aagggtgaa aagtatcgt ttcttaatc ctcccgaaa tttgacaga gacttggaa aaagtcctc caagagaag aaaatccta aatcttaaa acagtgctc acaatatcc ctccaaata attgatgtt ttggaatac atccacagt aaaggctat atgcactcc gatatcaag gcatctaat attcttcta agtaaccaa aataagccc aaaccaacc gtctgtaaa cgagtgtca agcggctgc acacgagta tcaagcggc tgcacgttc accagtcgt ctccgtgca tgtcgcaaa aaaccaacc agaatcact agaaactta aggccgaac gctaaagtc gccttttat ctcgacgac ataccaaat tttaatcaa atcttcact aattgtgtg tatgaaaaa ccaacaaca acacaggac cagatttat ctccttgat tacggtctg gcgtccaaa tttctaaca tcaaacggc gaacataaa ccgttttgc gttgacgaa cgtaaagct cacgctggg actgtttta ttttgttcg cgtgatgct cacaagggg gtgcattcg cgacgctca gatttggag tctttgggg tacaatatt atttattgg ctgactggg actctgcca tgggctggt gatgcagac aacccggaa atcgtagcc aagaagaag cagagagct atggctgat gtggagtat tttatgaac tattgtttc agtgaatat cctaagttt ctggaagaa tattttaag tatattcag aaactggaa tttcaggaa aagcctgac tacagctac tgcaagaag ctctttagg gagagtttg aaaacgaac ggttacaaa gacgattcg cgctttgac tttgacaat gtgcaaaga aacaaatta ttgaagccg actagaatc aggaagaga actaaggaa aatatcagg gcacggaaa tttatccac acaatatca cgtctacct ctacaatca aacatcccc attaaaccg ctactgaga aagaaagtg aaaaataag aaagtaaaa cctaaggca aattggttt ggggagctc actgaccct gagataata ctgaagcaa gctcgtaag aatagcgaa aatactgac gcaaactta gctagtaac gttgcggaa ttagatata aatgccttg aacccaacg tacgctatg attgaagtt tataataaa tgtagggag aggatagac gcatcaccc acttacaag agtgacagt tgtatagag accgtggat ggttacaca tctgcgatg atgtctgtg ttagagaaa atgaaaaag aggagagag cttgagttg gaagaaatg gctgtgaat ctcagtaat acaaggagt ggcagagtt cagaaaaga attcgtaaa cccaatagg aaatatgaa tctccagac tggtcgcct cctcagaca cgaacctcg aaaccagtg ataaagaag gatcaagca cgtaaggta aaaagtggg gttttgagg aggacttac agtttaaga ggttaagtt taactactc gtttttacg ttcgtgtat taactatta agactgcaa aatttgtga ctagaatac tgagttgtt tgtttcaaa aaatgtgca gtagatttg attgtaaat gaatagttt gttgattgt aataaataa acagttggc gctccaatc aattatcaa ctgaaatta tttctttaa ttgtgctcc atataagta cattagtat gattcgctt gtaccgcgc caattgttt cttaagtct gaagttata gttaattcg gcagttgtg tttgttctt gatttatat tatcgtttt aattcatct cagcgcaaa atagtgtaa aattcttgt ggtaaattt actgtgatt ttgacttat tgtgaaata aaacaat
