Status
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Gene information
General information
TC032344
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC003069
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 2793501..2797936
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032344
    iBeetle screen iB_07944

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032344 RA
      MAEVHIIGQ ITEAKDFPK ENLFCKWYL QVGNNWKVI 
      SGKKEGQTQ VTSPQFGKV CKWSYPIDI HLATAGLQG 
      WPKIYLQVY HLDWLGRSH IFGYGFITV PTTPGTHIL 
      DCYTWRPFG TIKDRFVQY FLGGGYQIK NPDLIFSSG 
      ERYKLSTEA MGVVSFELC VVLRNFSNY GVEYDTSLE 
      NVTTIQSSE TNVKKGTTS PILSTTAVP TTTCKANSS 
      CFANSTNNS VTVPPTQQT TALVTTPIP ASFFHFDIK 
      TSERSSKNL CICDLTIDY CDLNCCCDK DCSLENKLV 
      FDHCEEDFR QYDTRYCQY VKHIYVNKT PHEFQINQN 
      GLFCIVRSN LPASYIVQR KQPLKSLQD AQFERQNKF 
      NWPKPTHED SQIFNYFNY TYGKPLLIL RNNTIENLQ 
      FLKSFVVTS CVVKEDIKY LIDVKSSCS HTNISADNY 
      YLKADTFFR NMTIIANPG LLNVSKHKN FLKNCTKDV 
      CLNVRPKIC NEDFEDCVQ ISPNDTRIK SSCKVNIAN 
      NELFCINLV QNVRYNFYH NGSSGLIKV ELYLILKSA 
      TFYYHQEME FSQTIEVNF IWWNHTLNY SSHLSGNPG 
      YLVGKPILI ANIVSINVP INNTNSTSN ATSYKQQLQ 
      LYRNPSDFT ENFLVLPFV NSEGVCVLS KHKYTPVEF 
      GYNVYLRCE INDYLWIKN LSAKNVCIA VQNTIFKYW 
      ALKVTNGTL SNRVIALFG NAYIYNIGD WLRPMFEND 
      LKDILNRTW GEFDRQNKT LKCGNITTL LITEIFHAR 
      VDFDDIINQ EKILATTFQ FDNFQNFTF ALNHTLQFY 
      IKLGAQVMF HDITTKKMR KLVDPPSLK IRLPHDFFY 
      PFVKIDNHG VRQALFMNN NSIDRICKN CGDIVNRYC 
      CRYQKRCCE YLNPSGCPT PASPEARIS CRTPQFCRS 
      STDCPMFKQ CCDTSICGA TCMKPTFSG YRY
    CDS sequence
    • >TC032344 RA
      atggcagaa gtgcacata attgggcaa ataactgaa 
      gccaaagat tttccaaag gagaatttg ttctgtaaa 
      tggtatctc caagtaggt aataactgg aaagttatt 
      tctgggaag aaggaaggt caaactcaa gtaacatca 
      cctcagttt ggtaaagtt tgcaagtgg tcttatcca 
      atagatatt cacctagcc actgctgga cttcaaggt 
      tggcctaag atttatttg caagtttat cacttagat 
      tggctaggc cggagtcac attttcggt tacggtttc 
      ataacagtt cctacaact cccgggacc catattttg 
      gactgctac acttggaga ccatttggt acaataaaa 
      gatcgtttt gtgcagtat tttctggga ggtgggtat 
      caaataaaa aatccagac ttgattttc tcttcgggg 
      gaacgttat aaactctct acggaggct atgggagtt 
      gtttcattt gaactgtgt gttgtactt agaaatttc 
      agcaattat ggagtggaa tatgacaca agtttagaa 
      aacgtgacc acaatccag tcaagtgaa acaaatgtt 
      aaaaagggc acaacgtca ccaattttg tccacaacc 
      gctgttcca acaactact tgtaaagcc aacagttct 
      tgttttgca aattcaact aataattca gtaactgtt 
      cctccaact caacaaaca acggcttta gtcacaacc 
      ccgattcct gcgagcttt tttcatttt gatataaaa 
      acatcggaa cgatcaagc aagaatttg tgtatttgt 
      gatttaact atcgactac tgtgatctg aattgctgt 
      tgcgataaa gactgttcc ttagaaaat aaacttgta 
      ttcgatcat tgtgaagaa gatttccgt caatatgat 
      actagatat tgccaatac gtaaaacat atttacgtt 
      aataagaca cctcatgag tttcaaatt aaccaaaac 
      ggactattt tgtattgtg cgtagtaat ttaccagca 
      agttacata gtccaaaga aagcaaccg ttgaagtca 
      ttacaagat gctcaattc gagagacaa aataaattt 
      aattggcca aagcctaca catgaagat agtcaaata 
      ttcaattat ttcaactat acttatgga aaaccttta 
      ttgatttta agaaataac acaattgaa aacttacaa 
      tttcttaag agttttgtg gtaactagt tgcgtggtc 
      aaagaagat ataaaatac ttaattgac gtcaagagc 
      agctgtagt catacgaac atatccgca gataattat 
      tatctcaaa gccgacaca ttttttaga aacatgaca 
      ataattgca aatcctggt ttgctaaat gtgtctaaa 
      cacaaaaat tttctcaag aattgtaca aaagatgtt 
      tgtctaaat gttcggcca aaaatatgc aacgaagac 
      tttgaagac tgcgtccaa atatcgcca aacgacacg 
      agaataaaa tcgagctgc aaagttaac attgcaaac 
      aacgaactg ttttgtatt aatttagta caaaacgtt 
      agatacaat ttttatcat aatggcagc agcggtttg 
      ataaaagtc gaactttac ttgattctg aaaagtgcc 
      actttttat taccaccag gaaatggag ttttcccaa 
      acgattgaa gttaatttt atttggtgg aaccatact 
      ttgaactat tcatcgcac cttagtgga aatccggga 
      tatttagtt ggcaagcca atcttaatc gcaaatatc 
      gtttctata aatgttcct ataaataat acgaactcg 
      actagcaat gcaacaagt tacaaacaa caattacag 
      ctatatcga aatccttcg gatttcacc gaaaatttc 
      ctcgtttta ccgtttgta aattcagag ggtgtgtgc 
      gttttgagc aaacacaaa tatactccg gttgagttc 
      ggttataac gtgtatcta agatgcgaa attaatgat 
      tatctttgg atcaaaaat ctctcggca aaaaatgta 
      tgcattgcc gtacagaac acaatattc aaatactgg 
      gccttaaaa gtaacaaat ggtacatta agtaatcga 
      gtgattgca ctgttcggt aacgcatat atttacaat 
      attggcgat tggttgaga ccaatgttt gaaaacgat 
      cttaaagac attctaaac cgaacatgg ggcgaattt 
      gaccgtcaa aataaaact ctcaaatgt ggtaacatc 
      acaacacta cttataacg gagattttt catgcgaga 
      gttgatttc gacgacatt atcaatcag gaaaagatt 
      ttagcgact acctttcag tttgataat tttcaaaac 
      tttactttt gctttaaac catacttta cagttttat 
      attaaacta ggtgcacaa gttatgttt cacgacatc 
      actactaag aaaatgagg aaactcgtt gatccaccc 
      agtctgaaa attcgcttg cctcatgat ttcttttat 
      cctttcgtg aaaatagat aatcatggt gttaggcaa 
      gcactattt atgaataat aacagtatt gacagaatt 
      tgcaaaaat tgtggtgat atcgtcaat agatattgc 
      tgcagatac cagaaacga tgttgtgaa tatttaaat 
      ccgtctggt tgtcctacc ccagcaagt ccagaagct 
      cggataagt tgtcgaacc ccccagttt tgtcggtca 
      agtactgat tgtcccatg tttaaacaa tgctgtgat 
      acgtccata tgtggagca acatgcatg aagccaaca 
      ttttctgga tacagatat tga
    mRNA sequence
    • >TC032344 RA
      cacaaaact gacccccgc ccttaaaat atttgaaaa 
      tcgttgcta ggcaaccgg aaaacagta ttgtaaaca 
      aaaatattg tttttatgt ttggcattt tcttcaggt 
      ggttctttt atttcagtt ttgtttgtt gtgcaaaca 
      gttatcatt tgatggtat tctggcctt atggcagaa 
      gtgcacata attgggcaa ataactgaa gccaaagat 
      tttccaaag gagaatttg ttctgtaaa tggtatctc 
      caagtaggt aataactgg aaagttatt tctgggaag 
      aaggaaggt caaactcaa gtaacatca cctcagttt 
      ggtaaagtt tgcaagtgg tcttatcca atagatatt 
      cacctagcc actgctgga cttcaaggt tggcctaag 
      atttatttg caagtttat cacttagat tggctaggc 
      cggagtcac attttcggt tacggtttc ataacagtt 
      cctacaact cccgggacc catattttg gactgctac 
      acttggaga ccatttggt acaataaaa gatcgtttt 
      gtgcagtat tttctggga ggtgggtat caaataaaa 
      aatccagac ttgattttc tcttcgggg gaacgttat 
      aaactctct acggaggct atgggagtt gtttcattt 
      gaactgtgt gttgtactt agaaatttc agcaattat 
      ggagtggaa tatgacaca agtttagaa aacgtgacc 
      acaatccag tcaagtgaa acaaatgtt aaaaagggc 
      acaacgtca ccaattttg tccacaacc gctgttcca 
      acaactact tgtaaagcc aacagttct tgttttgca 
      aattcaact aataattca gtaactgtt cctccaact 
      caacaaaca acggcttta gtcacaacc ccgattcct 
      gcgagcttt tttcatttt gatataaaa acatcggaa 
      cgatcaagc aagaatttg tgtatttgt gatttaact 
      atcgactac tgtgatctg aattgctgt tgcgataaa 
      gactgttcc ttagaaaat aaacttgta ttcgatcat 
      tgtgaagaa gatttccgt caatatgat actagatat 
      tgccaatac gtaaaacat atttacgtt aataagaca 
      cctcatgag tttcaaatt aaccaaaac ggactattt 
      tgtattgtg cgtagtaat ttaccagca agttacata 
      gtccaaaga aagcaaccg ttgaagtca ttacaagat 
      gctcaattc gagagacaa aataaattt aattggcca 
      aagcctaca catgaagat agtcaaata ttcaattat 
      ttcaactat acttatgga aaaccttta ttgatttta 
      agaaataac acaattgaa aacttacaa tttcttaag 
      agttttgtg gtaactagt tgcgtggtc aaagaagat 
      ataaaatac ttaattgac gtcaagagc agctgtagt 
      catacgaac atatccgca gataattat tatctcaaa 
      gccgacaca ttttttaga aacatgaca ataattgca 
      aatcctggt ttgctaaat gtgtctaaa cacaaaaat 
      tttctcaag aattgtaca aaagatgtt tgtctaaat 
      gttcggcca aaaatatgc aacgaagac tttgaagac 
      tgcgtccaa atatcgcca aacgacacg agaataaaa 
      tcgagctgc aaagttaac attgcaaac aacgaactg 
      ttttgtatt aatttagta caaaacgtt agatacaat 
      ttttatcat aatggcagc agcggtttg ataaaagtc 
      gaactttac ttgattctg aaaagtgcc actttttat 
      taccaccag gaaatggag ttttcccaa acgattgaa 
      gttaatttt atttggtgg aaccatact ttgaactat 
      tcatcgcac cttagtgga aatccggga tatttagtt 
      ggcaagcca atcttaatc gcaaatatc gtttctata 
      aatgttcct ataaataat acgaactcg actagcaat 
      gcaacaagt tacaaacaa caattacag ctatatcga 
      aatccttcg gatttcacc gaaaatttc ctcgtttta 
      ccgtttgta aattcagag ggtgtgtgc gttttgagc 
      aaacacaaa tatactccg gttgagttc ggttataac 
      gtgtatcta agatgcgaa attaatgat tatctttgg 
      atcaaaaat ctctcggca aaaaatgta tgcattgcc 
      gtacagaac acaatattc aaatactgg gccttaaaa 
      gtaacaaat ggtacatta agtaatcga gtgattgca 
      ctgttcggt aacgcatat atttacaat attggcgat 
      tggttgaga ccaatgttt gaaaacgat cttaaagac 
      attctaaac cgaacatgg ggcgaattt gaccgtcaa 
      aataaaact ctcaaatgt ggtaacatc acaacacta 
      cttataacg gagattttt catgcgaga gttgatttc 
      gacgacatt atcaatcag gaaaagatt ttagcgact 
      acctttcag tttgataat tttcaaaac tttactttt 
      gctttaaac catacttta cagttttat attaaacta 
      ggtgcacaa gttatgttt cacgacatc actactaag 
      aaaatgagg aaactcgtt gatccaccc agtctgaaa 
      attcgcttg cctcatgat ttcttttat cctttcgtg 
      aaaatagat aatcatggt gttaggcaa gcactattt 
      atgaataat aacagtatt gacagaatt tgcaaaaat 
      tgtggtgat atcgtcaat agatattgc tgcagatac 
      cagaaacga tgttgtgaa tatttaaat ccgtctggt 
      tgtcctacc ccagcaagt ccagaagct cggataagt 
      tgtcgaacc ccccagttt tgtcggtca agtactgat 
      tgtcccatg tttaaacaa tgctgtgat acgtccata 
      tgtggagca acatgcatg aagccaaca ttttctgga 
      tacagatat tgaaaaaag tggtttaaa ctccttttt 
      tttctataa ttcgtgtca gcatctgac ataaaaagc 
      caatttgta atacgtttt gataattga gttatgcta 
      taattaata gtgtcgtaa tcgtaaaac ataattgat 
      tattctaat gggagaagc agtaaaata tggcttgta 
      tccagagaa gttttatct cgaatctcg aaaataaat 
      catgaatta ataaattat aattgtttt a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY