Status
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Gene information
General information
TC016199 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 4517463..4520267
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC016199
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_07940

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC016199 RA
      MPLPFDPSS NLTKSFNET RIQVTKNQR HDFYFWEEK 
      RGRFGTLFD QEIKNATEN MTGDFIATY NETFTYYAT 
      DSNATYNQL CAYDALDTD RINYCTLYD KICAHGNPD 
      GNTTNCHCR RRGEISTHF CELKCDNWT ETNSTTCNE 
      CEEIFHAYK DPTIRLWFN YHTREISVG LGSYLRLLP 
      ISESQAIVY CFTDADHST HDITYRYNL SRPSDLKYK 
      TATYNLEPV NDSIGHYWC QGFKFDLGP VSSPQIIAE 
      GKLHRESFT MNVTVREDE RTYPLTIHE KTEQELEDI 
      INNKSLIQE IKFLRLYER QTGHNSTNV TLSYRLYLN 
      ATNNTMMDV LDQVTTIFN QSDAPNIKN FTLSPSYCL 
      PDTTITNGI TLTWGKTLL GSTTVPNEL CLQNNTKPV 
      TRTCQGEYL YGTYWSEVK GTCVNNSGE LFGSPHTIQ 
      LHNLTYSHC EPNEKINQL NEMLQESKT PITIDAHFM 
      AKIFENVTS SQTLQVTQV LETASKIMN MPRDVLQEA 
      QTMLNSTDM ILYSLDTII AGYNLTDDY FSLTSGQIY 
      TQISNISSN ITGAAFYET KTGRRLQPL RNQTTPDYN 
      DTTLKLCAY LTPKTIAHN RNAKLVITV FLTDSLFNP 
      QKTTTGYTT SVYVTPNGV DTWVEEPVV MIRPFRKYR 
      AVLMCNYWS YGRNQLWYN LKGSWEHED KTPACTLGQ 
      MCPCPFVRM NPFGILVGD EDDVILSIV TATGCALSA 
      LGSLAVIVT AVVYKQWRE KSGTKILLN FVLTNLAQN 
      IFLGVSSGV NSYTQITTC VTIGALLHY SVCSQFAWM 
      LIIGYLQYM RYVEVFYVV HNNFVIWAS VVAWILPIV 
      PVITVLQMN DAYYLQDNF CYLTNRPLF YGVYVPVSI 
      TLTSGPVKG QYLVA
    CDS sequence
    • >TC016199 RA
      atgccacta ccattcgac ccatcatcc aatttaaca 
      aaatccttc aacgaaacc agaatccaa gtaacaaaa 
      aaccaaaga cacgacttc tacttctgg gaggaaaaa 
      cgcggcaga tttggcaca ctctttgac caagaaata 
      aaaaatgca accgaaaat atgaccggt gacttcata 
      gccacctac aacgaaact tttacatac tatgcaacc 
      gacagtaac gccacctat aaccagtta tgcgcctac 
      gacgcgctt gacactgac cgcataaac tactgcaca 
      ctgtatgac aaaatttgt gcacacgga aaccctgat 
      ggaaacaca actaactgt cattgcaga cgtagaggt 
      gaaattagt acgcatttt tgcgaatta aagtgtgac 
      aattggacg gaaacaaat agtacgacg tgtaacgaa 
      tgtgaggaa atttttcac gcttacaaa gatcccaca 
      attcggttg tggttcaac taccacacg agagaaata 
      tcagtgggg cttggatcc tacttgaga ctacttccg 
      atcagtgaa agccaagcg attgtctat tgtttcaca 
      gatgctgat cactccact catgacatt acttacagg 
      tataactta agtcgcccc agtgacttg aaatataaa 
      acggccacc tataatcta gagcctgtt aatgactca 
      attggacac tactggtgt caagggttc aaatttgat 
      ttgggtcct gtatctagt ccacaaatt atcgccgag 
      ggaaaactc cacagagag tcattcaca atgaatgtg 
      actgtacgt gaagacgaa aggacttat cctttgaca 
      atacacgaa aaaacagaa caagaactt gaagacatc 
      atcaataat aagagtttg attcaagaa attaaattt 
      ttgcgcttg tatgagcgt caaactggc cacaactcc 
      accaatgtc acactgagt tatcggctg tacttgaac 
      gcaaccaac aatacaatg atggacgtc cttgatcaa 
      gtgaccaca attttcaac caatcagat gcccccaac 
      atcaaaaac ttcacactt tcgcccagc tattgtctc 
      cctgatacg accataacc aatggtata accctcaca 
      tgggggaaa accctgctg ggaagcacc acagtcccg 
      aatgaactg tgcctccaa aacaacaca aaaccagtg 
      acacgtaca tgccaaggc gaatacctt tatggcact 
      tattggtcg gaggttaaa ggcacgtgt gtcaacaac 
      agcggcgaa ttattcggt tcacctcac actatacaa 
      ttacacaac ctcacatat tcacactgt gaaccaaac 
      gaaaaaatc aaccaattg aatgaaatg ctccaagag 
      agcaaaaca ccaataaca atcgatgcg cacttcatg 
      gcgaaaatt ttcgaaaac gtgacttcg tcccaaacc 
      ctccaagtg acccaagtc ttggaaacg gccagtaaa 
      ataatgaac atgccacgt gacgtgcta caagaagcc 
      caaacaatg ctcaactca accgacatg atcctatac 
      agtcttgat acaataatc gccggttat aacctaacc 
      gatgactat ttcagccta acctctggc caaatctac 
      acccaaatc agcaacatc tcgagcaac ataaccggc 
      gccgccttc tacgaaacc aaaaccggg cgtcggtta 
      caaccgctc cgtaaccaa accacgccc gactacaac 
      gacaccacc ttgaaactc tgcgcctat ctaacgccc 
      aaaaccatc gcccacaac cgtaacgcc aagttggtt 
      ataaccgtc ttcctaacc gactcactc ttcaacccc 
      caaaaaacc acaaccggt tacacgacc agtgtttac 
      gtcacacca aatggggtt gatacgtgg gttgaggaa 
      ccggtcgtg atgattcgg ccgtttcgg aaatacagg 
      gccgtactt atgtgtaac tactggtcg tatgggcgc 
      aaccaattg tggtacaat ttgaagggt tcgtgggaa 
      catgaggat aaaacgccg gcttgcact ttgggccaa 
      atgtgtccc tgtccgttc gttcgcatg aacccgttt 
      gggattttg gtgggggat gaggatgac gttattttg 
      agtattgtt acggcgacc ggttgcgcc ctttcggcg 
      ctgggcagt ttggcggtg attgtaacc gcagttgtg 
      tataaacag tggcgcgaa aaatccggc actaaaata 
      ctcctcaat tttgtgtta acaaacttg gcacagaat 
      attttcctt ggggtgagt agtggggtt aatagttac 
      acgcaaatc accacgtgt gttacaata ggggcgtta 
      ctacactac tcggtttgt tcgcagttt gcgtggatg 
      ctgatcatt ggctacttg cagtacatg cgctacgtt 
      gaggtgttt tacgtcgtg cacaacaat ttcgtaatt 
      tgggcgtcg gttgtagcc tggattttg ccaatagtt 
      ccggttata accgtttta caaatgaac gacgcctac 
      tatctccaa gacaatttc tgctacttg accaacaga 
      ccgctcttt tacggtgtt tatgtcccg gtttccatc 
      actttgact tccggaccg gttaaggga caatatctc 
      gtggcttaa
    mRNA sequence
    • >TC016199 RA
      tgctacatc tacaaccga acctgtccc tacccaatg 
      ccactacca ttcgaccca tcatccaat ttaacaaaa 
      tccttcaac gaaaccaga atccaagta acaaaaaac 
      caaagacac gacttctac ttctgggag gaaaaacgc 
      ggcagattt ggcacactc tttgaccaa gaaataaaa 
      aatgcaacc gaaaatatg accggtgac ttcatagcc 
      acctacaac gaaactttt acatactat gcaaccgac 
      agtaacgcc acctataac cagttatgc gcctacgac 
      gcgcttgac actgaccgc ataaactac tgcacactg 
      tatgacaaa atttgtgca cacggaaac cctgatgga 
      aacacaact aactgtcat tgcagacgt agaggtgaa 
      attagtacg catttttgc gaattaaag tgtgacaat 
      tggacggaa acaaatagt acgacgtgt aacgaatgt 
      gaggaaatt tttcacgct tacaaagat cccacaatt 
      cggttgtgg ttcaactac cacacgaga gaaatatca 
      gtggggctt ggatcctac ttgagacta cttccgatc 
      agtgaaagc caagcgatt gtctattgt ttcacagat 
      gctgatcac tccactcat gacattact tacaggtat 
      aacttaagt cgccccagt gacttgaaa tataaaacg 
      gccacctat aatctagag cctgttaat gactcaatt 
      ggacactac tggtgtcaa gggttcaaa tttgatttg 
      ggtcctgta tctagtcca caaattatc gccgaggga 
      aaactccac agagagtca ttcacaatg aatgtgact 
      gtacgtgaa gacgaaagg acttatcct ttgacaata 
      cacgaaaaa acagaacaa gaacttgaa gacatcatc 
      aataataag agtttgatt caagaaatt aaatttttg 
      cgcttgtat gagcgtcaa actggccac aactccacc 
      aatgtcaca ctgagttat cggctgtac ttgaacgca 
      accaacaat acaatgatg gacgtcctt gatcaagtg 
      accacaatt ttcaaccaa tcagatgcc cccaacatc 
      aaaaacttc acactttcg cccagctat tgtctccct 
      gatacgacc ataaccaat ggtataacc ctcacatgg 
      gggaaaacc ctgctggga agcaccaca gtcccgaat 
      gaactgtgc ctccaaaac aacacaaaa ccagtgaca 
      cgtacatgc caaggcgaa tacctttat ggcacttat 
      tggtcggag gttaaaggc acgtgtgtc aacaacagc 
      ggcgaatta ttcggttca cctcacact atacaatta 
      cacaacctc acatattca cactgtgaa ccaaacgaa 
      aaaatcaac caattgaat gaaatgctc caagagagc 
      aaaacacca ataacaatc gatgcgcac ttcatggcg 
      aaaattttc gaaaacgtg acttcgtcc caaaccctc 
      caagtgacc caagtcttg gaaacggcc agtaaaata 
      atgaacatg ccacgtgac gtgctacaa gaagcccaa 
      acaatgctc aactcaacc gacatgatc ctatacagt 
      cttgataca ataatcgcc ggttataac ctaaccgat 
      gactatttc agcctaacc tctggccaa atctacacc 
      caaatcagc aacatctcg agcaacata accggcgcc 
      gccttctac gaaaccaaa accgggcgt cggttacaa 
      ccgctccgt aaccaaacc acgcccgac tacaacgac 
      accaccttg aaactctgc gcctatcta acgcccaaa 
      accatcgcc cacaaccgt aacgccaag ttggttata 
      accgtcttc ctaaccgac tcactcttc aacccccaa 
      aaaaccaca accggttac acgaccagt gtttacgtc 
      acaccaaat ggggttgat acgtgggtt gaggaaccg 
      gtcgtgatg attcggccg tttcggaaa tacagggcc 
      gtacttatg tgtaactac tggtcgtat gggcgcaac 
      caattgtgg tacaatttg aagggttcg tgggaacat 
      gaggataaa acgccggct tgcactttg ggccaaatg 
      tgtccctgt ccgttcgtt cgcatgaac ccgtttggg 
      attttggtg ggggatgag gatgacgtt attttgagt 
      attgttacg gcgaccggt tgcgccctt tcggcgctg 
      ggcagtttg gcggtgatt gtaaccgca gttgtgtat 
      aaacagtgg cgcgaaaaa tccggcact aaaatactc 
      ctcaatttt gtgttaaca aacttggca cagaatatt 
      ttccttggg gtgagtagt ggggttaat agttacacg 
      caaatcacc acgtgtgtt acaataggg gcgttacta 
      cactactcg gtttgttcg cagtttgcg tggatgctg 
      atcattggc tacttgcag tacatgcgc tacgttgag 
      gtgttttac gtcgtgcac aacaatttc gtaatttgg 
      gcgtcggtt gtagcctgg attttgcca atagttccg 
      gttataacc gttttacaa atgaacgac gcctactat 
      ctccaagac aatttctgc tacttgacc aacagaccg 
      ctcttttac ggtgtttat gtcccggtt tccatcact 
      ttgacttcc ggaccggtt aagggacaa tatctcgtg 
      gcttaattt acgtctcgt cattttact gttcttcct 
      catgggttt gtactgggt gtttggggt gtcggcacg 
      tttgacgca a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY