Status
Please wait ...
Gene information
General information
TC003336 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 1429268..1433345
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC003336
    iBeetle screen iB_07939

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003336 RA
      MFYRRLKRI LPVVFALRQ RHLAFLVCA AANEVSESC 
DQRGDSLVK CTAKNQFVN LGNMRKGAE GFKLEVSDG 
LLLYYDRFS FYSQKVVMA LHEKNLAFD SKIVNLIKN 
EQYQPWYLF LNPRGEVPV LQDTGKIIP DSARIIDYL 
EDNFSNGDT PRLIPMDQG AEVRQRVTH FRHILQKAP 
AGMLTTGAM LNQHLLKNP KSPFFAPIR KALANAEKN 
SGKHLREYA EKNPEAKEI LLKKASVRE EQYKQLSDE 
KNFRDVLNQ VHTILDEIE AELEKHKGD KEDWWLCSD 
RFTIADVEL TILLIRISQ LGLEHIFWS SGKRPCIEK 
YYERVKARD SFKKTVPGT LVLLKTILM SQAPIVFGV 
VAAVALVVG GAIIAKKYI A
    CDS sequence
    • >TC003336 RA
      atgttttat cgacgatta aagcgtatc ctgccagta 
gtgtttgcg ctgcgtcaa cgacacttg gcgtttttg 
gtttgtgca gccgcgaac gaggttagc gagagttgt 
gatcagcgc ggcgatagt cttgtcaag tgcaccgcc 
aaaaatcag tttgtcaac ttgggaaat atgcggaaa 
ggcgccgag gggttcaag cttgaggtc tccgatggc 
ttgctgttg tactacgat cgcttctcg ttttattcc 
caaaaggtg gttatggct ctccacgag aagaacctc 
gcctttgat agcaaaata gtaaacttg atcaaaaac 
gagcaatat cagccttgg tacctcttc ctgaaccct 
cgaggcgaa gtccctgtc cttcaggac actggcaag 
ataattcca gactcggcc cgcattatc gactacttg 
gaggacaat ttcagcaat ggggacact cccagactt 
atccccatg gatcaggga gctgaagtc aggcagagg 
gtcacccat ttcagacac atcctgcag aaagcgcct 
gcaggaatg ctaacaact ggcgctatg ctcaaccaa 
cacctttta aaaaatccg aaaagtccg ttttttgca 
ccgattcga aaggcactt gccaatgct gaaaagaac 
agtgggaag catttgcgg gagtatgcg gagaagaat 
ccagaggcc aaagaaata ctgttgaag aaagccagt 
gttagggaa gaacagtac aaacaactg tctgatgag 
aagaatttc agggacgtc ctaaatcaa gtacatacg 
attctggat gagattgaa gcagagttg gagaagcac 
aagggtgat aaggaagac tggtggctg tgttctgat 
agatttaca atagccgat gttgagttg acgatttta 
ttgatcagg atcagtcag ttagggctt gaacacatc 
ttttggagt tcgggaaag aggccttgt attgaaaaa 
tactacgag agggtcaag gcaagggat tcgttcaag 
aagacagtt ccagggacc ttagtgttg ctaaaaaca 
atactcatg tcacaagca ccgatcgtt ttcggggtt 
gtggctgct gtggcgttg gtcgttgga ggggcgatc 
atcgcaaag aagtacatt gcttag
    mRNA sequence
    • >TC003336 RA
      cttcagaaa aaaaacacg aatgcgttt tcttgccga 
tttattgtg taaaaaaat gtttggaat tagaaatta 
cataacagc caaaataaa caaatgaca taatttgag 
tctgcgctg tggaggcgg ttagcagga atgtttggc 
aactttcct gcctatctg tacataaca atagcccca 
tttagccca ataaacctg tctcggtgc gtcacgtcg 
ccatactta aatcagaaa ttatagcaa atcgctccc 
aagccagcg ccctctcct ctgagaagg ctcgcttgc 
cggtagttg gagcgtgtt ttgcgtggc gccgcgacg 
cccccgacg cttccaaat cgaatggcg cgtaattga 
aatcctcaa tcagtaaaa tttgatccc agatcgcgt 
ttttgtgat ctagcgatt cgaaaacta gtgtgacgt 
cgcgatgtt ttatcgacg attaaagcg tatcctgcc 
agtagtgtt tgcgctgcg tcaacgaca cttggcgtt 
tttggtttg tgcagccgc gaacgaggt tagcgagag 
ttgtgatca gcgcggcga tagtcttgt caagtgcac 
cgccaaaaa tcagtttgt caacttggg aaatatgcg 
gaaaggcgc cgaggggtt caagcttga ggtctccga 
tggcttgct gttgtacta cgatcgctt ctcgtttta 
ttcccaaaa ggtggttat ggctctcca cgagaagaa 
cctcgcctt tgatagcaa aatagtaaa cttgatcaa 
aaacgagca atatcagcc ttggtacct cttcctgaa 
ccctcgagg cgaagtccc tgtccttca ggacactgg 
caagataat tccagactc ggcccgcat tatcgacta 
cttggagga caatttcag caatgggga cactcccag 
acttatccc catggatca gggagctga agtcaggca 
gagggtcac ccatttcag acacatcct gcagaaagc 
gcctgcagg aatgctaac aactggcgc tatgctcaa 
ccaacacct tttaaaaaa tccgaaaag tccgttttt 
tgcaccgat tcgaaaggc acttgccaa tgctgaaaa 
gaacagtgg gaagcattt gcgggagta tgcggagaa 
gaatccaga ggccaaaga aatactgtt gaagaaagc 
cagtgttag ggaagaaca gtacaaaca actgtctga 
tgagaagaa tttcaggga cgtcctaaa tcaagtaca 
tacgattct ggatgagat tgaagcaga gttggagaa 
gcacaaggg tgataagga agactggtg gctgtgttc 
tgatagatt tacaatagc cgatgttga gttgacgat 
tttattgat caggatcag tcagttagg gcttgaaca 
catcttttg gagttcggg aaagaggcc ttgtattga 
aaaatacta cgagagggt caaggcaag ggattcgtt 
caagaagac agttccagg gaccttagt gttgctaaa 
aacaatact catgtcaca agcaccgat cgttttcgg 
ggttgtggc tgctgtggc gttggtcgt tggaggggc 
gatcatcgc aaagaagta cattgctta gatgagtcg 
ctgtgtggg tcagtgatg cgcgtattt tgttaagtt 
tctgttgat aggtggtac caaatattt ttatattga 
gaggtagtc gcggttgat ttattattc tactttact 
cccgaaaat aaatttcat tgaactag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY