Status
Please wait ...
Gene information
General information
TC003142 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 1207116..1210335
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003142
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_07935

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    barely above 50

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003142 RA
      MQNRNIILL VFIVIVLCF KVNQCTHLT GAFKSNEFF 
      KFLVKFSFQ KTDRHQADA THGYIFGNI TSDQNFPVP 
      VTFAVLQRS YFLEYYQNR VLYNKEEAC KRMFSKITT 
      HAYELQCNS KGQDYLRRI PCPKGKLCI DEVKDWLVV 
      KGHQFTYVI EDFNQPSSW YVSMVACYL NMTTCEWHH 
      YEPSKQYTI HYDLWLVNG NPNVSSYNT LTYQFSFDR 
      QNTLELYLL FWLCYMVLV PLQCHAVKT QKHPVTKLF 
      TASLLLDFI ALCLILIHT LKFALDGEG YPKLAMAGD 
      IFDILSRTS FMLLLLLLA KGWAVTRLE LSWKPLVFA 
      IWLCYGIVH ILLYVWNLT EVDIIEDID EYQTWPGWL 
      IIVFRSLIM VWFLYELRT TMLYEHNTQ KLNFLLHFG 
      ASSLVWFIY LPILALIAL HVSALWRFK LLLGITYSA 
      DCFAYCVMA HLLWPTRSE QYFLLKGPS SADIDELDE 
      FNEAPHIVN NSYSSLSTM SSIEICRFD EAPKNKAVS 
      A
    CDS sequence
    • >TC003142 RA
      atgcaaaac agaaacatc attttgttg gttttcatc 
      gtaatagtc ctgtgcttc aaagtaaat cagtgcacg 
      cacctgaca ggggcgttt aagagcaac gagtttttc 
      aaatttttg gttaaattc agctttcaa aaaacggac 
      agacaccaa gcagatgcg acgcatgga tacattttc 
      gggaacatt acgtccgac caaaacttc ccggtccct 
      gtcacgttt gcagtatta cagaggtcg tatttctta 
      gagtattac cagaacagg gttttgtac aacaaggag 
      gaggcgtgc aaacgcatg tttagcaag ataacgaca 
      catgcctac gagctacag tgcaactcc aagggacag 
      gactacttg cggaggatc ccttgcccg aagggaaaa 
      ctctgcatt gacgaggtt aaagattgg cttgtggtg 
      aaaggccac cagtttact tacgtgatc gaggatttt 
      aaccaacct tcgtcttgg tatgtttcc atggtggcc 
      tgttatctc aacatgacc acgtgtgag tggcaccat 
      tacgagcca tcaaagcaa tacaccata cattacgac 
      ctgtggcta gtgaatgga aacccaaac gtctcatct 
      tacaatact ttaacttac cagttttcg ttcgaccgc 
      cagaatacg ctagaactc taccttctt ttctggttg 
      tgttacatg gttttagtt cctttgcaa tgtcacgct 
      gttaaaacg caaaaacac ccagttacc aaacttttc 
      actgccagt ttgctctta gatttcata gctctgtgt 
      ttaattctc atccacacc ttgaaattt gcgcttgat 
      ggagaaggg tacccgaaa ctggccatg gctggggac 
      attttcgac attctttca aggacttcg tttatgctt 
      cttttgttg ctacttgcc aaagggtgg gcggtcacg 
      cgcctagaa ttgtcatgg aagcccctg gtttttgca 
      atttggttg tgttatggt atagtacac atacttctc 
      tatgtatgg aatttgact gaagtagac ataatagaa 
      gacattgac gaataccaa acatggcca ggttggctc 
      ataatagta tttaggtcc ttaataatg gtttggttt 
      ctgtacgaa ttgcgcaca acgatgttg tatgaacac 
      aacacgcaa aagttgaat tttttgcta cactttgga 
      gcgtctagt cttgtctgg ttcatatat ttgccaatc 
      ttagcttta atagcactc catgtgtca gctctgtgg 
      cgatttaag ctcctattg gggatcact tattcagcc 
      gactgcttt gcgtattgc gttatggcg catttgttg 
      tggcctacg cgctctgag cagtacttt ttgctgaaa 
      ggcccttcg tcggcggat atcgacgag ctggacgag 
      tttaacgaa gcgccgcac attgtgaac aattcctac 
      tcatcgcta agtacaatg agttcgata gagatttgc 
      cggtttgac gaagcacct aaaaataaa gccgtcagt 
      gcgtaa
    mRNA sequence
    • >TC003142 RA
      ttttcaaaa gaaaatgca aaacagaaa catcatttt 
      gttggtttt catcgtaat agtcctgtg cttcaaagt 
      aaatcagtg cacgcacct gacaggggc gtttaagag 
      caacgagtt tttcaaatt tttggttaa attcagctt 
      tcaaaaaac ggacagaca ccaagcaga tgcgacgca 
      tggatacat tttcgggaa cattacgtc cgaccaaaa 
      cttcccggt ccctgtcac gtttgcagt attacagag 
      gtcgtattt cttagagta ttaccagaa cagggtttt 
      gtacaacaa ggaggaggc gtgcaaacg catgtttag 
      caagataac gacacatgc ctacgagct acagtgcaa 
      ctccaaggg acaggacta cttgcggag gatcccttg 
      cccgaaggg aaaactctg cattgacga ggttaaaga 
      ttggcttgt ggtgaaagg ccaccagtt tacttacgt 
      gatcgagga ttttaacca accttcgtc ttggtatgt 
      ttccatggt ggcctgtta tctcaacat gaccacgtg 
      tgagtggca ccattacga gccatcaaa gcaatacac 
      catacatta cgacctgtg gctagtgaa tggaaaccc 
      aaacgtctc atcttacaa tactttaac ttaccagtt 
      ttcgttcga ccgccagaa tacgctaga actctacct 
      tcttttctg gttgtgtta catggtttt agttccttt 
      gcaatgtca cgctgttaa aacgcaaaa acacccagt 
      taccaaact tttcactgc cagtttgct cttagattt 
      catagctct gtgtttaat tctcatcca caccttgaa 
      atttgcgct tgatggaga agggtaccc gaaactggc 
      catggctgg ggacatttt cgacattct ttcaaggac 
      ttcgtttat gcttctttt gttgctact tgccaaagg 
      gtgggcggt cacgcgcct agaattgtc atggaagcc 
      cctggtttt tgcaatttg gttgtgtta tggtatagt 
      acacatact tctctatgt atggaattt gactgaagt 
      agacataat agaagacat tgacgaata ccaaacatg 
      gccaggttg gctcataat agtatttag gtccttaat 
      aatggtttg gtttctgta cgaattgcg cacaacgat 
      gttgtatga acacaacac gcaaaagtt gaatttttt 
      gctacactt tggagcgtc tagtcttgt ctggttcat 
      atatttgcc aatcttagc tttaatagc actccatgt 
      gtcagctct gtggcgatt taagctcct attggggat 
      cacttattc agccgactg ctttgcgta ttgcgttat 
      ggcgcattt gttgtggcc tacgcgctc tgagcagta 
      ctttttgct gaaaggccc ttcgtcggc ggatatcga 
      cgagctgga cgagtttaa cgaagcgcc gcacattgt 
      gaacaattc ctactcatc gctaagtac aatgagttc 
      gatagagat ttgccggtt tgacgaagc acctaaaaa 
      taaagccgt cagtgcgta aaacttgtg atttaattt 
      aaaatgtta gcacgacta acttggaaa tgattgtca 
      atttgtctg tacatagaa ttgttgaaa attttgacg 
      tgcagttac gtatgtgtg caaatattg atttatatt 
      tacatgttg ttgagcgtt tacaaatgt tttaaggat 
      cttatgatg aatatgatg attgccaaa gactgttgc 
      gttaaatat ttacaaaaa tttatcatc tcatgtaac 
      acctgtaca tagtcaaag ttaatgtgt ttttacatt 
      gtggatatt attttattg ctcaatctt tcgttttgt 
      gatgttaat taaggagta ggagttatg tgtatgttt 
      gtctgtaca cagttgtgc aaatattta catcctttt 
      ttccctgtt cacgatttt ttggaattg cgcttagag 
      ttgtgtgca gcacataat tttcactca ccgcggttg 
      taactagca atttctagt gccaagtgt ttttacaga 
      ttggatatt tacgtttta aattaccta ttagtatgt 
      aggtctttg tattatatt ttttgtact gccctgtga 
      aacaagtta ttttagtgt gtgatactt gcaatttcc 
      ttttactta gattgtcaa tttgaaaca tcgatcaat 
      ttagtgtaa agtattctt ataacacaa taaatgatt 
      acaataact gaagcattt ttggataaa aatgtgtgc 
      agaaaaaca attatttat tagtctcat aatacatat 
      tcccgtaac attattaat aataaaatt tgtattaac 
      tatgtgact aatccttgt tttctcttg tgttcatac 
      tctgacata cattttcta aaatcagaa acttgccgc 
      tggcatttc ctccaatct tgcgtttct gctatgcat 
      tcctagata atttaatct aagaataat tggaaacga 
      attaattag caatttaaa cctggactt tgtaatgat 
      gattaatac atccggttt tctttaaca tttcctcaa 
      caggatcag tgatttctt gtttgccat gtgcaagac 
      tcatggtgg agaagtttg cctgaattt tagcaaaaa 
      gtggaaata tttagtggc aaattaccc actagttcg 
      taattcact tatttattt gggttatta tgtctacaa 
      acattaaaa aatatatct ttttaaagg ttaaagtat 
      aaaaaccac agaataaac actgttgca ggttatgta 
      atacattca aacagaagg cgggaaggt caagcagat 
      tcaacatct gtgaaatct ccaaaaaag ttattaata 
      ttgtatggc tattgttac gtttaaaaa acaataaat 
      acaccgaaa aaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY