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Gene information
General information
TC003173 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 714520..715678
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003173
    iBeetle screen iB_07932

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 6 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003173 RA
      MSTSGIKTE SNDSNDSKP VFKRKNRKQ LRQRRPSDG 
      SDREEDLEE VSTKLEEMK ELQNLRKRP HGVNALGLA 
      LGTKITIED ECISKDPFK VKSGGMVNM QALKSGKVK 
      QVDDAYDTG IGTQFSVET NKRDEDEEM MKFIEEELS 
      KKKRKVEPQ EQAEAENKS AYTSPEEAA LRAVPDHLR 
      ESSTKRSEE MLSNQMLNG IPEVDLGIE AKIKNIEAT 
      EEAKLRLLW EKQNKKDGP SPFVPTNMA VNFVQHNRF 
      NIDAEVAKK KAKLDDKVE EATEKPKKK DERATDDYH 
      YEKFKKQFR RY
    CDS sequence
    • >TC003173 RA
      atgtcaaca agtgggatt aaaaccgaa tctaacgat 
      tcaaatgac tcgaaaccc gtttttaaa cgtaaaaac 
      cgtaaacag ctgcgccaa aggcgcccc tcggatggt 
      agtgaccga gaggaggac ctggaggag gtcagcacc 
      aaactagaa gaaatgaag gagttacaa aacttgcgt 
      aagcgcccc catggagtc aacgccttg gggctagcc 
      ctaggtacc aaaatcacc atcgaggac gagtgcatt 
      tcaaaggac cctttcaag gtgaagtcc ggcggcatg 
      gtcaacatg caagccctt aaaagcggc aaagtcaag 
      caagtcgat gatgcgtac gacactggc atagggacc 
      cagttttcg gtcgagacg aataaacgt gacgaagac 
      gaggaaatg atgaagttt attgaagaa gagttgtcg 
      aagaagaag cgcaaggtc gagccccag gagcaggcc 
      gaggctgag aacaagtcg gcgtacacc agccccgag 
      gaggcggct ttgagggca gtcccggac catttgagg 
      gagtcgtcg acgaagagg tcggaggag atgttgtcc 
      aatcagatg ttgaatggg attccggaa gtggacctg 
      gggattgag gccaagatt aagaatatt gaagctacg 
      gaagaggcg aagttgagg ctgctgtgg gagaagcag 
      aataagaag gacgggcct tcgcctttt gtgccgacg 
      aatatggcg gtgaatttt gtgcagcac aatcgattt 
      aatatagac gcagaggtt gctaaaaag aaggccaag 
      ttggatgat aaagtggag gaggctacg gaaaagccg 
      aagaaaaag gatgagagg gccaccgat gattatcac 
      tacgaaaag ttcaaaaaa caatttagg agatattag
    mRNA sequence
    • >TC003173 RA
      aatggtgcc acacgaatt atgaattat tttgtgttt 
      tgtgagcca gtctcgagt gtcttgtgg tggttttgg 
      tcgtttttg gtgtagtta ttggggcgt cataacctc 
      aaattcgtc atgtcaaca agtgggatt aaaaccgaa 
      tctaacgat tcaaatgac tcgaaaccc gtttttaaa 
      cgtaaaaac cgtaaacag ctgcgccaa aggcgcccc 
      tcggatggt agtgaccga gaggaggac ctggaggag 
      gtcagcacc aaactagaa gaaatgaag gagttacaa 
      aacttgcgt aagcgcccc catggagtc aacgccttg 
      gggctagcc ctaggtacc aaaatcacc atcgaggac 
      gagtgcatt tcaaaggac cctttcaag gtgaagtcc 
      ggcggcatg gtcaacatg caagccctt aaaagcggc 
      aaagtcaag caagtcgat gatgcgtac gacactggc 
      atagggacc cagttttcg gtcgagacg aataaacgt 
      gacgaagac gaggaaatg atgaagttt attgaagaa 
      gagttgtcg aagaagaag cgcaaggtc gagccccag 
      gagcaggcc gaggctgag aacaagtcg gcgtacacc 
      agccccgag gaggcggct ttgagggca gtcccggac 
      catttgagg gagtcgtcg acgaagagg tcggaggag 
      atgttgtcc aatcagatg ttgaatggg attccggaa 
      gtggacctg gggattgag gccaagatt aagaatatt 
      gaagctacg gaagaggcg aagttgagg ctgctgtgg 
      gagaagcag aataagaag gacgggcct tcgcctttt 
      gtgccgacg aatatggcg gtgaatttt gtgcagcac 
      aatcgattt aatatagac gcagaggtt gctaaaaag 
      aaggccaag ttggatgat aaagtggag gaggctacg 
      gaaaagccg aagaaaaag gatgagagg gccaccgat 
      gattatcac tacgaaaag ttcaaaaaa caatttagg 
      agatattag ccttttgta gtgtttttt atttttgaa 
      taaattatt tattttgat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY