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Gene information
General information
TC003192 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 464206..465893
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003192
    iBeetle screen iB_07931

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003192 RA
      MFLVRNLKQ FSTFNKRCC VYFTKVSYS NAHSKFVTP 
      KSQAKQIGP LGWFLLVIP ASTFALGTW QVQRKKWKE 
      DLIAKLHNL TEADPVQLP TDLNELEKL EYRPVHVRG 
      EFLHDKELY LGPRTLILK GDSATKSQL MSTTTKQNQ 
      GFLVITPFK LADRNETIL INRGWVPSK CKNPATRDK 
      GQVKGVVDV VGIVRLQEN RPTFIPKNQ EGSNQWFYR 
      DLNQMAKVT GALPVLLEA TTDFDTSEG PIGGQTRVT 
      LRNEHLSYI LTWYSLSAA TSYLWYKQF LSRVK
    CDS sequence
    • >TC003192 RA
      atgttctta gtccgaaat ttaaaacag ttttcaacg 
      tttaataaa cgttgttgc gtgtatttc accaaagtt 
      tcgtattct aatgcacat tcaaagttt gtcacccca 
      aagagccaa gccaaacaa atcggccct ttgggttgg 
      ttcctcctg gtcatccca gcctcaact ttcgcactt 
      gggacatgg caagtgcaa cgtaaaaaa tggaaagag 
      gatttaatc gcaaaatta cataacttg actgaggct 
      gatcccgtg caattacca accgattta aatgagttg 
      gaaaagttg gaatatcgg ccggttcac gtccggggc 
      gagtttctc cacgacaaa gagttatat ttaggcccc 
      agaacttta attttaaaa ggcgattcg gcaactaaa 
      agtcaactc atgtcgacc acaacaaag caaaaccag 
      ggtttttta gtcattaca ccgtttaaa ttagctgat 
      cggaatgaa acgatttta ataaatcgg ggttgggta 
      ccttccaag tgcaaaaat ccagcgaca agggacaag 
      ggacaggtg aagggggtt gttgatgtt gttggtata 
      gtacgtctt caggaaaat cgccccact ttcatacca 
      aaaaaccag gaaggcagc aaccaatgg ttttatcgc 
      gatttgaat caaatggcc aaggttaca ggagcgctt 
      cctgtgctt ctggaagcc actactgat tttgatact 
      tctgagggc cccattggg gggcaaact cgggtcact 
      ttacgcaac gaacacttg tcatatatt ctcacgtgg 
      tatagttta agcgcagcg acgtcttac ctgtggtac 
      aaacaattc ttaagtcgg gttaaataa
    mRNA sequence
    • >TC003192 RA
      cccaacaaa aaatgttct tagtccgaa atttaaaac 
      agttttcaa cgtttaata aacgttgtt gcgtgtatt 
      tcaccaaag tttcgtatt ctaatgcac attcaaagt 
      ttgtcaccc caaagagcc aagccaaac aaatcggcc 
      ctttgggtt ggttcctcc tggtcatcc cagcctcaa 
      ctttcgcac ttgggacat ggcaagtgc aacgtaaaa 
      aatggaaag aggatttaa tcgcaaaat tacataact 
      tgactgagg ctgatcccg tgcaattac caaccgatt 
      taaatgagt tggaaaagt tggaatatc ggccggttc 
      acgtccggg gcgagtttc tccacgaca aagagttat 
      atttaggcc ccagaactt taattttaa aaggcgatt 
      cggcaacta aaagtcaac tcatgtcga ccacaacaa 
      agcaaaacc agggttttt tagtcatta caccgttta 
      aattagctg atcggaatg aaacgattt taataaatc 
      ggggttggg taccttcca agtgcaaaa atccagcga 
      caagggaca agggacagg tgaaggggg ttgttgatg 
      ttgttggta tagtacgtc ttcaggaaa atcgcccca 
      ctttcatac caaaaaacc aggaaggca gcaaccaat 
      ggttttatc gcgatttga atcaaatgg ccaaggtta 
      caggagcgc ttcctgtgc ttctggaag ccactactg 
      attttgata cttctgagg gccccattg gggggcaaa 
      ctcgggtca ctttacgca acgaacact tgtcatata 
      ttctcacgt ggtatagtt taagcgcag cgacgtctt 
      acctgtggt acaaacaat tcttaagtc gggttaaat 
      aaatgtgaa aaattcgct gataaaatt tattttcct 
      actttgtac catgtaaat aacagcttg taacacatg 
      aattttctc atataaatc tttcataaa aagaggaag 
      aaattcaac agtctatta attaaacgt gtggatatt 
      tgaaacatc tttggcaaa catttgtct agtcaacaa 
      tgtgtaaag cattcaatt ggacagaga gaaatttcg 
      agatgggac atgcagaga atctgtcgc cacagtcac 
      aacgtttat ttggcactt tgcacattt aatacttta 
      ggcgcaata atacaatgt ggcaacaaa tttcaggta 
      atcgtaaca gtgtgtttt aatagagca tgtttggtc 
      ctaagtgaa cgagaaaat agttaaaaa aaaacagag 
      agcaataaa ctttaccaa aaattgatt tcgataatc 
      cattacata ataaattcg aatatgtta aaataagaa 
      atagataca tcaatatta tttactaaa catatataa 
      aaatatata aaattataa aataacata tactttacg 
      ttagatttt ctcttttta tcgtacatg ctccaatgg 
      cagtcaatc gaaataaaa aattcagat atggggttt 
      tcaaattta cttactcta acttattcg tattacttc 
      ttcttcttg cccgaagaa ttgctttta aaattatta 
      ctaatacca tttgttgca tgcgggacg ttaccattg 
      gccattc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY