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Gene information
General information
TC003247 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 308189..309903
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003247
    iBeetle screen iB_07929

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 4
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 20 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003247 RA
      MVYIHFPAN FTEEELMLQ AKYQKLKKK KKALQALKA 
      PKPEPEKPI TPKRPAEAR DAREVAKKL IKSGAIPAI 
      SKQQKQPEQ GFKRPRGLE RKLISDKIV SGYQPFSAV 
      QMDDGPDNP ENKPPRIKN LYDTFANAR DREERGLTE 
      KQREKPDKP RQGNTIYVS GYKITEEFL KKHFSTIGN 
      IVNISMEIE KNRGFVTFD KVEAAERAI AEMDGSMVS 
      GIQLKVTLA RRQPQIEPI NDASSSAAW ATLASSHSQ 
      KGNHRDKRN LVSYDDMFD
    CDS sequence
    • >TC003247 RA
      atggtttac attcatttt ccggcaaat ttcactgaa 
      gaggagctc atgctccaa gccaaatac cagaaactt 
      aagaaaaag aaaaaggct ttgcaagcc ctgaaagcc 
      cccaaaccc gagcccgaa aagcccatt acgcctaaa 
      cggccagcg gaggctcga gatgctcgt gaggttgcg 
      aaaaaactt atcaaatcc ggtgccatt ccggccatt 
      tccaaacaa caaaaacag ccagaacag ggctttaaa 
      cggcctaga ggcttggaa agaaaactg atttcggat 
      aaaattgtg agtggttat cagcctttt tcggctgtg 
      cagatggac gatgggcct gataacccc gaaaataag 
      ccgccaagg attaagaat ttgtatgat acttttgct 
      aatgcgcgt gatagggaa gagaggggt ttgactgag 
      aaacagagg gaaaagccg gataaaccc aggcaaggg 
      aatacgatt tatgtgtca gggtataag ataacggag 
      gagtttttg aagaagcat ttttcaacg attggaaat 
      attgtgaat atttcaatg gagattgag aaaaatcgc 
      gggtttgtc acgtttgat aaggtggaa gccgccgaa 
      cgggccata gctgagatg gatgggagt atggtttcg 
      gggattcag ctcaaagtc actctagcg agacgacag 
      ccccaaata gagccaata aatgacgca tcatcgagc 
      gcagcgtgg gccactcta gcgtcaagt cattctcaa 
      aaaggcaat cacagggat aagagaaat ttagtgtcg 
      tatgatgat atgtttgac taa
    mRNA sequence
    • >TC003247 RA
      ttcagtttg caaattgga caagttttt ttttgatta 
      aacgatggt ttacattca ttttccggc aaatttcac 
      tgaagagga gctcatgct ccaagccaa ataccagaa 
      acttaagaa aaagaaaaa ggctttgca agccctgaa 
      agcccccaa acccgagcc cgaaaagcc cattacgcc 
      taaacggcc agcggaggc tcgagatgc tcgtgaggt 
      tgcgaaaaa acttatcaa atccggtgc cattccggc 
      catttccaa acaacaaaa acagccaga acagggctt 
      taaacggcc tagaggctt ggaaagaaa actgatttc 
      ggataaaat tgtgagtgg ttatcagcc tttttcggc 
      tgtgcagat ggacgatgg gcctgataa ccccgaaaa 
      taagccgcc aaggattaa gaatttgta tgatacttt 
      tgctaatgc gcgtgatag ggaagagag gggtttgac 
      tgagaaaca gagggaaaa gccggataa acccaggca 
      agggaatac gatttatgt gtcagggta taagataac 
      ggaggagtt tttgaagaa gcatttttc aacgattgg 
      aaatattgt gaatatttc aatggagat tgagaaaaa 
      tcgcgggtt tgtcacgtt tgataaggt ggaagccgc 
      cgaacgggc catagctga gatggatgg gagtatggt 
      ttcggggat tcagctcaa agtcactct agcgagacg 
      acagcccca aatagagcc aataaatga cgcatcatc 
      gagcgcagc gtgggccac tctagcgtc aagtcattc 
      tcaaaaagg caatcacag ggataagag aaatttagt 
      gtcgtatga tgatatgtt tgactaaac ttacgtaag 
      gtgatttat taagaaaaa acccgctta attataata 
      gtattaaca ataaaaaat t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY