Status
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Gene information
General information
TC003215 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 69067..75484
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003215
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_07925

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Adult morphology
    abdominal odoriferous glands content split - Number of animals showing the phenotype: 2 (13 dpi)
    Days post injection



    abdominal odoriferous glands partially colour lighter - Number of animals showing the phenotype: 2 (13 dpi)
    Days post injection


    abdominal odoriferous glands size decreased - Number of animals showing the phenotype: 2 (13 dpi)
    Days post injection



    abdominal odoriferous glands content decreased - Number of animals showing the phenotype: 2 (13 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003215 RA
      MSHPVPAPR SNAPDVKKP VPTPRKSTK LKPEPPEEH 
      TNTFSRRVR SLSNASRQI AEDIGELVQ DKKKAMIAG 
      TRQSVRRIT KRFNSYHSE SPQDPEPAE DDFGDTLDI 
      FNSISFQSP LSSTSAIYN NVEAEEFNA PPPTHPPPP 
      LPQECIYDA PASISGGST SNSEQSNLG NYPPNYESV 
      FPVYSNSDS DLSRSGSWR FYDPVNKVE NIYNDLDVT 
      VQRTECDVN SNVSEFSTE VRNSLYENH TIAARVQAE 
      KKCRESVIM QFDPLRTGS TVGEDVCFP QSVLPSEDI 
      KMLEEYFQG DLYGNVSIT RTLDEWSIS NDSEPEEFI 
      TPPMPPVRI DSLPEEEQS PREKSRSQW FTSSDSVKE 
      NKEEPKKFG WLKQIKGAL EKAPEVVKG VRNKDTVVQ 
      RPELEIKSF VHKKGMLYK VQSGPVEDL FGEYSGRWC 
      ILQNSNFIC YSDSTCDTV KEHFPAESI LSVQILQDP 
      KFKYKYDND DLHCFELNT TGKSRGGHI YGTRDISER 
      RVWMQSIAE SLTKKFPVK ITTDYTRMG WAYLREGVG 
      GRWVGAWLM LSHRTLYYA VDNQPTKTM DLRKVRCMV 
      LQVYQENDN NPRTNDKGP NIVVDTPGV TLYLRMWTS 
      RETKVWCHI VKLAAHNNG AHLDQQQLT KNDIPVLVE 
      KCINFIYTH GSMTEGIYR RPGTNTAVA EILAKFRKD 
      AWAVQLTID KYTEHDVAT ALKRFIRDI PEPVLTNSQ 
      RQYLHQVSL VKNKDERIR MYKAALDQL PPISYKTAK 
      KLLGHLHFI ASQSKKNLM NVDNLAAVW GPSLMHQEE 
      KDNNSVGNT KHVQNTVVV RQLLELYRD IFPEDPAEI 
      EKEKVMLQV LEKHTKALQ GAANNAKKA GDFRVWIYL 
      HNKEGQPMN VAIGPNKTA YEVCVELSP KINMPVHEL 
      VLEEVVLND RLQRPVHFS EKVLDIVLR WSYWEESDR 
      KDNHLVLLP IAKYSDFIH DKSTPVSAE LKFSDNRSR 
      NFKLYTFEF SQAKLNCFK DKTCEEKLF TYNIEDIMW 
      YLGHESKKN PQSRWTITF IETKMHPQR TKNCPYFGN 
      VLVWGDPQT RAKWLGAML KARYRESLI QPAMHINLM 
      VN
    CDS sequence
    • >TC003215 RA
      atgagccat ccggtacca gcccccagg tccaatgcg 
      cccgatgtc aaaaagcca gtccccaca ccacgcaaa 
      agcacaaaa ctaaaacct gaaccccct gaagagcac 
      accaacacc ttctccaga agggttcgc tctttgagc 
      aatgcttca cgacaaatc gccgaagat atcggcgaa 
      ttggtccag gataagaag aaagctatg attgcggga 
      actagacaa agtgttcgg agaatcacc aagcgtttt 
      aattcgtac cactcagag tccccacag gaccctgag 
      cccgctgaa gatgatttt ggcgatacc ctcgatatt 
      ttcaactcg atcagtttc caatcgccg ctcagttca 
      acttccgcc atttacaat aatgtggag gcggaggag 
      ttcaatgcg ccgcctccg acacatcca ccgcctcca 
      ttgccccag gagtgcatc tacgatgcc ccagcttcc 
      atttctggg ggcagtaca agcaattcc gaacagtca 
      aatctaggg aattatcca ccgaactat gaaagtgtt 
      tttcccgtt tattcaaac agtgattcg gatttatcc 
      agatccggt tcgtggaga ttctacgat ccggtcaat 
      aaagtcgaa aatatttac aatgatttg gatgtgaca 
      gttcaaagg actgaatgc gatgttaat tcgaatgtg 
      agcgaattt tccacggaa gtacgaaat tcgttgtac 
      gaaaatcat acaatagcg gcaagagta caagcagag 
      aagaaatgt cgagagagt gttataatg cagtttgat 
      ccgttgagg actggtagc actgttggt gaagatgtg 
      tgtttcccc caatccgtt cttccatcc gaagacatc 
      aaaatgctc gaggaatat tttcaaggc gatctttat 
      ggcaatgtt tccatcacg cgaactttg gatgaatgg 
      agcatttca aatgattcg gaaccggaa gaatttatc 
      acacctcca atgcctcca gttcgtatc gattctctc 
      ccagaagaa gaacaatca cctcgagaa aaatcgcga 
      agtcaatgg tttactagt tccgattct gttaaagaa 
      aataaggaa gaaccgaaa aaattcgga tggttgaaa 
      cagatcaag ggggcactt gaaaaagct cccgaagtg 
      gtcaaaggt gtgagaaat aaagataca gtggtccag 
      agacccgaa ttagaaatt aaaagtttt gtacacaag 
      aaaggaatg ttgtataag gtgcaaagt ggacctgtt 
      gaagatttg ttcggggag tacagtgga agatggtgc 
      attttacag aattcaaat tttatttgc tactcggat 
      agcacttgt gatacagtc aaggagcat tttccggcc 
      gagagcatt ctaagtgtg caaattttg caagatcct 
      aaatttaaa tacaaatac gacaacgac gaccttcac 
      tgtttcgaa ctaaacacg acgggaaaa agtcgcgga 
      ggtcacatc tatggaaca cgcgacatt tctgaacga 
      cgcgtttgg atgcaatcc attgccgaa agtctaaca 
      aagaaattc cctgtgaaa atcactacc gattacaca 
      cgtatgggc tgggcatac cttcgggaa ggtgttggt 
      ggacgatgg gtgggcgct tggcttatg ctatcccac 
      aggactttg tactatgcc gttgataat caaccgact 
      aaaacgatg gatttgaga aaagtacga tgtatggta 
      ttacaagta taccaagag aatgataac aatccaagg 
      actaatgat aaagggccg aatattgtg gtggataca 
      cctggtgta acgctttat ttacgcatg tggacgtct 
      agggaaaca aaggtttgg tgccatatt gttaagctg 
      gcagcacac aacaatggt gctcatttg gatcagcaa 
      cagttgacg aaaaatgat attccagtt ttagtggaa 
      aagtgcatc aattttatt tacactcac ggaagcatg 
      actgaggga atttacaga cgtccaggc actaacact 
      gcagttgca gaaattttg gcgaaattc cgtaaagac 
      gcttgggca gtacaatta acaattgat aaatataca 
      gaacatgat gttgcgact gctttaaag cgattcatc 
      agagacata cctgaacca gttctcaca aacagccaa 
      cggcaatat ttacatcaa gtttcattg gtgaagaat 
      aaagacgaa aggataaga atgtacaaa gcagctctc 
      gatcagctc ccgcctatt tcatacaaa acggcgaaa 
      aaactcctc ggacatctc cactttatt gcgtcacaa 
      agcaaaaaa aatctgatg aatgtcgat aatttggct 
      gcagtttgg ggaccgagt ttaatgcat caagaagaa 
      aaggataat aattcggtt ggaaacacc aaacacgtt 
      caaaacact gtagttgta cgacagttg ttggaatta 
      tatagggat atttttcca gaggatccc gccgaaatt 
      gaaaaggaa aaagtcatg ttacaagtg ttggaaaag 
      cacactaag gcactacaa ggtgcagct aataatgca 
      aaaaaagct ggcgatttt cgggtgtgg atttatttg 
      cataataag gaaggacag ccaatgaat gttgcaatt 
      ggcccaaat aagactgcg tatgaagtt tgcgtggag 
      ctaagtccg aaaatcaat atgccggta cacgaattg 
      gttttggag gaagttgtt ttgaacgat agattacaa 
      cgacctgtg catttttcc gaaaaggtt ttggatatc 
      gtccttcgg tggagttat tgggaggaa tccgatcga 
      aaagacaat catttagtc cttcttcct atagcaaaa 
      tattccgac tttattcac gacaaatcg acaccagtt 
      tcagcagaa ctcaaattt tccgataac agatcaagg 
      aatttcaag ttgtacacg tttgaattt tcccaagcc 
      aagcttaac tgttttaag gataaaacg tgtgaggag 
      aaactcttc acttacaac attgaagac atcatgtgg 
      tatttaggt cacgaatct aaaaaaaat ccacaatcg 
      cggtggaca atcacattt atcgaaact aaaatgcat 
      ccacaacgt actaaaaac tgtccttat ttcggtaat 
      gtgttggtt tggggtgat ccacaaacc agagccaaa 
      tggttgggt gcaatgttg aaagcgaga tatagggag 
      agtttgata cagccagct atgcatatc aatttaatg 
      gttaattga
    mRNA sequence
    • >TC003215 RA
      aaagtgatt atgcgtcac aaaaacaga attccttca 
      gttgatcgt ccaccagca gcactgcca acagtgata 
      gtgtcctca aatctcaaa aaaatgccg ttttgttaa 
      tttattgga attttcaac gtgtcgtga cattcagtg 
      attgctttt ccataattg cgataatga taaggatat 
      gtcgtttcg tgcgatgaa acaacagag tcaacaatt 
      taataaaaa tgagccatc cggtaccag cccccaggt 
      ccaatgcgc ccgatgtca aaaagccag tccccacac 
      cacgcaaaa gcacaaaac taaaacctg aaccccctg 
      aagagcaca ccaacacct tctccagaa gggttcgct 
      ctttgagca atgcttcac gacaaatcg ccgaagata 
      tcggcgaat tggtccagg ataagaaga aagctatga 
      ttgcgggaa ctagacaaa gtgttcgga gaatcacca 
      agcgtttta attcgtacc actcagagt ccccacagg 
      accctgagc ccgctgaag atgattttg gcgataccc 
      tcgatattt tcaactcga tcagtttcc aatcgccgc 
      tcagttcaa cttccgcca tttacaata atgtggagg 
      cggaggagt tcaatgcgc cgcctccga cacatccac 
      cgcctccat tgccccagg agtgcatct acgatgccc 
      cagcttcca tttctgggg gcagtacaa gcaattccg 
      aacagtcaa atctaggga attatccac cgaactatg 
      aaagtgttt ttcccgttt attcaaaca gtgattcgg 
      atttatcca gatccggtt cgtggagat tctacgatc 
      cggtcaata aagtcgaaa atatttaca atgatttgg 
      atgtgacag ttcaaagga ctgaatgcg atgttaatt 
      cgaatgtga gcgaatttt ccacggaag tacgaaatt 
      cgttgtacg aaaatcata caatagcgg caagagtac 
      aagcagaga agaaatgtc gagagagtg ttataatgc 
      agtttgatc cgttgagga ctggtagca ctgttggtg 
      aagatgtgt gtttccccc aatccgttc ttccatccg 
      aagacatca aaatgctcg aggaatatt ttcaaggcg 
      atctttatg gcaatgttt ccatcacgc gaactttgg 
      atgaatgga gcatttcaa atgattcgg aaccggaag 
      aatttatca cacctccaa tgcctccag ttcgtatcg 
      attctctcc cagaagaag aacaatcac ctcgagaaa 
      aatcgcgaa gtcaatggt ttactagtt ccgattctg 
      ttaaagaaa ataaggaag aaccgaaaa aattcggat 
      ggttgaaac agatcaagg gggcacttg aaaaagctc 
      ccgaagtgg tcaaaggtg tgagaaata aagatacag 
      tggtccaga gacccgaat tagaaatta aaagttttg 
      tacacaaga aaggaatgt tgtataagg tgcaaagtg 
      gacctgttg aagatttgt tcggggagt acagtggaa 
      gatggtgca ttttacaga attcaaatt ttatttgct 
      actcggata gcacttgtg atacagtca aggagcatt 
      ttccggccg agagcattc taagtgtgc aaattttgc 
      aagatccta aatttaaat acaaatacg acaacgacg 
      accttcact gtttcgaac taaacacga cgggaaaaa 
      gtcgcggag gtcacatct atggaacac gcgacattt 
      ctgaacgac gcgtttgga tgcaatcca ttgccgaaa 
      gtctaacaa agaaattcc ctgtgaaaa tcactaccg 
      attacacac gtatgggct gggcatacc ttcgggaag 
      gtgttggtg gacgatggg tgggcgctt ggcttatgc 
      tatcccaca ggactttgt actatgccg ttgataatc 
      aaccgacta aaacgatgg atttgagaa aagtacgat 
      gtatggtat tacaagtat accaagaga atgataaca 
      atccaagga ctaatgata aagggccga atattgtgg 
      tggatacac ctggtgtaa cgctttatt tacgcatgt 
      ggacgtcta gggaaacaa aggtttggt gccatattg 
      ttaagctgg cagcacaca acaatggtg ctcatttgg 
      atcagcaac agttgacga aaaatgata ttccagttt 
      tagtggaaa agtgcatca attttattt acactcacg 
      gaagcatga ctgagggaa tttacagac gtccaggca 
      ctaacactg cagttgcag aaattttgg cgaaattcc 
      gtaaagacg cttgggcag tacaattaa caattgata 
      aatatacag aacatgatg ttgcgactg ctttaaagc 
      gattcatca gagacatac ctgaaccag ttctcacaa 
      acagccaac ggcaatatt tacatcaag tttcattgg 
      tgaagaata aagacgaaa ggataagaa tgtacaaag 
      cagctctcg atcagctcc cgcctattt catacaaaa 
      cggcgaaaa aactcctcg gacatctcc actttattg 
      cgtcacaaa gcaaaaaaa atctgatga atgtcgata 
      atttggctg cagtttggg gaccgagtt taatgcatc 
      aagaagaaa aggataata attcggttg gaaacacca 
      aacacgttc aaaacactg tagttgtac gacagttgt 
      tggaattat atagggata tttttccag aggatcccg 
      ccgaaattg aaaaggaaa aagtcatgt tacaagtgt 
      tggaaaagc acactaagg cactacaag gtgcagcta 
      ataatgcaa aaaaagctg gcgattttc gggtgtgga 
      tttatttgc ataataagg aaggacagc caatgaatg 
      ttgcaattg gcccaaata agactgcgt atgaagttt 
      gcgtggagc taagtccga aaatcaata tgccggtac 
      acgaattgg ttttggagg aagttgttt tgaacgata 
      gattacaac gacctgtgc atttttccg aaaaggttt 
      tggatatcg tccttcggt ggagttatt gggaggaat 
      ccgatcgaa aagacaatc atttagtcc ttcttccta 
      tagcaaaat attccgact ttattcacg acaaatcga 
      caccagttt cagcagaac tcaaatttt ccgataaca 
      gatcaagga atttcaagt tgtacacgt ttgaatttt 
      cccaagcca agcttaact gttttaagg ataaaacgt 
      gtgaggaga aactcttca cttacaaca ttgaagaca 
      tcatgtggt atttaggtc acgaatcta aaaaaaatc 
      cacaatcgc ggtggacaa tcacattta tcgaaacta 
      aaatgcatc cacaacgta ctaaaaact gtccttatt 
      tcggtaatg tgttggttt ggggtgatc cacaaacca 
      gagccaaat ggttgggtg caatgttga aagcgagat 
      atagggaga gtttgatac agccagcta tgcatatca 
      atttaatgg ttaattgag ttaccaaat ttacttatt 
      ttttactta aaacgcacc tttttatgt acgtagcta 
      ataatgatt tctgtgtat ttcttttat ttgcgtgac 
      agtaagtgg caaaatttt aacggttaa aaaggcgaa 
      acgatttta tttatgcgg ctgtgaagt tttacaaaa 
      gttttaaca aaaaatttc cgtaatttt atttattgc 
      attttatta tgactaccg aatttacaa tctgtgatt 
      atgtggaaa agcaattac agaaaccag tgttgcgta 
      aaattgtcg tcattttaa ttccttgta atgatttaa 
      tgaggagaa ataatatta aagtcttcc aaaaaagcc 
      agcaaaact cgtttcaga ttgttatga gcacgtgag 
      ttcaacact tgattgctt ataagaata tgaaactga 
      cgtttttag gttttttat ctgcaatat acattatac 
      ataatcgct ttgattttt tttattcta taagtagtt 
      aattttttg gtgctcaag ccaaacgta tcgtataat 
      tttaattta gattttgca ataattgtg tacaaactt 
      tgtagccat tcctgttcg tagtcttgc cattgtgcc 
      aagtctgcc aactttaat aaaaaaagc acacacaat 
      tgtgatcat gtaaaacat ccactaatg tgaaaaatc 
      gatat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY