| General information | |||
| TC003215 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 69067..75484 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC003215 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_07925 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
abdominal odoriferous glands partially colour lighter - Number of animals showing the phenotype: 2 (13 dpi)
abdominal odoriferous glands size decreased - Number of animals showing the phenotype: 2 (13 dpi)
abdominal odoriferous glands content decreased - Number of animals showing the phenotype: 2 (13 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC003215 RA
MSHPVPAPR SNAPDVKKP VPTPRKSTK LKPEPPEEH TNTFSRRVR SLSNASRQI AEDIGELVQ DKKKAMIAG TRQSVRRIT KRFNSYHSE SPQDPEPAE DDFGDTLDI FNSISFQSP LSSTSAIYN NVEAEEFNA PPPTHPPPP LPQECIYDA PASISGGST SNSEQSNLG NYPPNYESV FPVYSNSDS DLSRSGSWR FYDPVNKVE NIYNDLDVT VQRTECDVN SNVSEFSTE VRNSLYENH TIAARVQAE KKCRESVIM QFDPLRTGS TVGEDVCFP QSVLPSEDI KMLEEYFQG DLYGNVSIT RTLDEWSIS NDSEPEEFI TPPMPPVRI DSLPEEEQS PREKSRSQW FTSSDSVKE NKEEPKKFG WLKQIKGAL EKAPEVVKG VRNKDTVVQ RPELEIKSF VHKKGMLYK VQSGPVEDL FGEYSGRWC ILQNSNFIC YSDSTCDTV KEHFPAESI LSVQILQDP KFKYKYDND DLHCFELNT TGKSRGGHI YGTRDISER RVWMQSIAE SLTKKFPVK ITTDYTRMG WAYLREGVG GRWVGAWLM LSHRTLYYA VDNQPTKTM DLRKVRCMV LQVYQENDN NPRTNDKGP NIVVDTPGV TLYLRMWTS RETKVWCHI VKLAAHNNG AHLDQQQLT KNDIPVLVE KCINFIYTH GSMTEGIYR RPGTNTAVA EILAKFRKD AWAVQLTID KYTEHDVAT ALKRFIRDI PEPVLTNSQ RQYLHQVSL VKNKDERIR MYKAALDQL PPISYKTAK KLLGHLHFI ASQSKKNLM NVDNLAAVW GPSLMHQEE KDNNSVGNT KHVQNTVVV RQLLELYRD IFPEDPAEI EKEKVMLQV LEKHTKALQ GAANNAKKA GDFRVWIYL HNKEGQPMN VAIGPNKTA YEVCVELSP KINMPVHEL VLEEVVLND RLQRPVHFS EKVLDIVLR WSYWEESDR KDNHLVLLP IAKYSDFIH DKSTPVSAE LKFSDNRSR NFKLYTFEF SQAKLNCFK DKTCEEKLF TYNIEDIMW YLGHESKKN PQSRWTITF IETKMHPQR TKNCPYFGN VLVWGDPQT RAKWLGAML KARYRESLI QPAMHINLM VN
- >TC003215 RA
atgagccat ccggtacca gcccccagg tccaatgcg cccgatgtc aaaaagcca gtccccaca ccacgcaaa agcacaaaa ctaaaacct gaaccccct gaagagcac accaacacc ttctccaga agggttcgc tctttgagc aatgcttca cgacaaatc gccgaagat atcggcgaa ttggtccag gataagaag aaagctatg attgcggga actagacaa agtgttcgg agaatcacc aagcgtttt aattcgtac cactcagag tccccacag gaccctgag cccgctgaa gatgatttt ggcgatacc ctcgatatt ttcaactcg atcagtttc caatcgccg ctcagttca acttccgcc atttacaat aatgtggag gcggaggag ttcaatgcg ccgcctccg acacatcca ccgcctcca ttgccccag gagtgcatc tacgatgcc ccagcttcc atttctggg ggcagtaca agcaattcc gaacagtca aatctaggg aattatcca ccgaactat gaaagtgtt tttcccgtt tattcaaac agtgattcg gatttatcc agatccggt tcgtggaga ttctacgat ccggtcaat aaagtcgaa aatatttac aatgatttg gatgtgaca gttcaaagg actgaatgc gatgttaat tcgaatgtg agcgaattt tccacggaa gtacgaaat tcgttgtac gaaaatcat acaatagcg gcaagagta caagcagag aagaaatgt cgagagagt gttataatg cagtttgat ccgttgagg actggtagc actgttggt gaagatgtg tgtttcccc caatccgtt cttccatcc gaagacatc aaaatgctc gaggaatat tttcaaggc gatctttat ggcaatgtt tccatcacg cgaactttg gatgaatgg agcatttca aatgattcg gaaccggaa gaatttatc acacctcca atgcctcca gttcgtatc gattctctc ccagaagaa gaacaatca cctcgagaa aaatcgcga agtcaatgg tttactagt tccgattct gttaaagaa aataaggaa gaaccgaaa aaattcgga tggttgaaa cagatcaag ggggcactt gaaaaagct cccgaagtg gtcaaaggt gtgagaaat aaagataca gtggtccag agacccgaa ttagaaatt aaaagtttt gtacacaag aaaggaatg ttgtataag gtgcaaagt ggacctgtt gaagatttg ttcggggag tacagtgga agatggtgc attttacag aattcaaat tttatttgc tactcggat agcacttgt gatacagtc aaggagcat tttccggcc gagagcatt ctaagtgtg caaattttg caagatcct aaatttaaa tacaaatac gacaacgac gaccttcac tgtttcgaa ctaaacacg acgggaaaa agtcgcgga ggtcacatc tatggaaca cgcgacatt tctgaacga cgcgtttgg atgcaatcc attgccgaa agtctaaca aagaaattc cctgtgaaa atcactacc gattacaca cgtatgggc tgggcatac cttcgggaa ggtgttggt ggacgatgg gtgggcgct tggcttatg ctatcccac aggactttg tactatgcc gttgataat caaccgact aaaacgatg gatttgaga aaagtacga tgtatggta ttacaagta taccaagag aatgataac aatccaagg actaatgat aaagggccg aatattgtg gtggataca cctggtgta acgctttat ttacgcatg tggacgtct agggaaaca aaggtttgg tgccatatt gttaagctg gcagcacac aacaatggt gctcatttg gatcagcaa cagttgacg aaaaatgat attccagtt ttagtggaa aagtgcatc aattttatt tacactcac ggaagcatg actgaggga atttacaga cgtccaggc actaacact gcagttgca gaaattttg gcgaaattc cgtaaagac gcttgggca gtacaatta acaattgat aaatataca gaacatgat gttgcgact gctttaaag cgattcatc agagacata cctgaacca gttctcaca aacagccaa cggcaatat ttacatcaa gtttcattg gtgaagaat aaagacgaa aggataaga atgtacaaa gcagctctc gatcagctc ccgcctatt tcatacaaa acggcgaaa aaactcctc ggacatctc cactttatt gcgtcacaa agcaaaaaa aatctgatg aatgtcgat aatttggct gcagtttgg ggaccgagt ttaatgcat caagaagaa aaggataat aattcggtt ggaaacacc aaacacgtt caaaacact gtagttgta cgacagttg ttggaatta tatagggat atttttcca gaggatccc gccgaaatt gaaaaggaa aaagtcatg ttacaagtg ttggaaaag cacactaag gcactacaa ggtgcagct aataatgca aaaaaagct ggcgatttt cgggtgtgg atttatttg cataataag gaaggacag ccaatgaat gttgcaatt ggcccaaat aagactgcg tatgaagtt tgcgtggag ctaagtccg aaaatcaat atgccggta cacgaattg gttttggag gaagttgtt ttgaacgat agattacaa cgacctgtg catttttcc gaaaaggtt ttggatatc gtccttcgg tggagttat tgggaggaa tccgatcga aaagacaat catttagtc cttcttcct atagcaaaa tattccgac tttattcac gacaaatcg acaccagtt tcagcagaa ctcaaattt tccgataac agatcaagg aatttcaag ttgtacacg tttgaattt tcccaagcc aagcttaac tgttttaag gataaaacg tgtgaggag aaactcttc acttacaac attgaagac atcatgtgg tatttaggt cacgaatct aaaaaaaat ccacaatcg cggtggaca atcacattt atcgaaact aaaatgcat ccacaacgt actaaaaac tgtccttat ttcggtaat gtgttggtt tggggtgat ccacaaacc agagccaaa tggttgggt gcaatgttg aaagcgaga tatagggag agtttgata cagccagct atgcatatc aatttaatg gttaattga
- >TC003215 RA
aaagtgatt atgcgtcac aaaaacaga attccttca gttgatcgt ccaccagca gcactgcca acagtgata gtgtcctca aatctcaaa aaaatgccg ttttgttaa tttattgga attttcaac gtgtcgtga cattcagtg attgctttt ccataattg cgataatga taaggatat gtcgtttcg tgcgatgaa acaacagag tcaacaatt taataaaaa tgagccatc cggtaccag cccccaggt ccaatgcgc ccgatgtca aaaagccag tccccacac cacgcaaaa gcacaaaac taaaacctg aaccccctg aagagcaca ccaacacct tctccagaa gggttcgct ctttgagca atgcttcac gacaaatcg ccgaagata tcggcgaat tggtccagg ataagaaga aagctatga ttgcgggaa ctagacaaa gtgttcgga gaatcacca agcgtttta attcgtacc actcagagt ccccacagg accctgagc ccgctgaag atgattttg gcgataccc tcgatattt tcaactcga tcagtttcc aatcgccgc tcagttcaa cttccgcca tttacaata atgtggagg cggaggagt tcaatgcgc cgcctccga cacatccac cgcctccat tgccccagg agtgcatct acgatgccc cagcttcca tttctgggg gcagtacaa gcaattccg aacagtcaa atctaggga attatccac cgaactatg aaagtgttt ttcccgttt attcaaaca gtgattcgg atttatcca gatccggtt cgtggagat tctacgatc cggtcaata aagtcgaaa atatttaca atgatttgg atgtgacag ttcaaagga ctgaatgcg atgttaatt cgaatgtga gcgaatttt ccacggaag tacgaaatt cgttgtacg aaaatcata caatagcgg caagagtac aagcagaga agaaatgtc gagagagtg ttataatgc agtttgatc cgttgagga ctggtagca ctgttggtg aagatgtgt gtttccccc aatccgttc ttccatccg aagacatca aaatgctcg aggaatatt ttcaaggcg atctttatg gcaatgttt ccatcacgc gaactttgg atgaatgga gcatttcaa atgattcgg aaccggaag aatttatca cacctccaa tgcctccag ttcgtatcg attctctcc cagaagaag aacaatcac ctcgagaaa aatcgcgaa gtcaatggt ttactagtt ccgattctg ttaaagaaa ataaggaag aaccgaaaa aattcggat ggttgaaac agatcaagg gggcacttg aaaaagctc ccgaagtgg tcaaaggtg tgagaaata aagatacag tggtccaga gacccgaat tagaaatta aaagttttg tacacaaga aaggaatgt tgtataagg tgcaaagtg gacctgttg aagatttgt tcggggagt acagtggaa gatggtgca ttttacaga attcaaatt ttatttgct actcggata gcacttgtg atacagtca aggagcatt ttccggccg agagcattc taagtgtgc aaattttgc aagatccta aatttaaat acaaatacg acaacgacg accttcact gtttcgaac taaacacga cgggaaaaa gtcgcggag gtcacatct atggaacac gcgacattt ctgaacgac gcgtttgga tgcaatcca ttgccgaaa gtctaacaa agaaattcc ctgtgaaaa tcactaccg attacacac gtatgggct gggcatacc ttcgggaag gtgttggtg gacgatggg tgggcgctt ggcttatgc tatcccaca ggactttgt actatgccg ttgataatc aaccgacta aaacgatgg atttgagaa aagtacgat gtatggtat tacaagtat accaagaga atgataaca atccaagga ctaatgata aagggccga atattgtgg tggatacac ctggtgtaa cgctttatt tacgcatgt ggacgtcta gggaaacaa aggtttggt gccatattg ttaagctgg cagcacaca acaatggtg ctcatttgg atcagcaac agttgacga aaaatgata ttccagttt tagtggaaa agtgcatca attttattt acactcacg gaagcatga ctgagggaa tttacagac gtccaggca ctaacactg cagttgcag aaattttgg cgaaattcc gtaaagacg cttgggcag tacaattaa caattgata aatatacag aacatgatg ttgcgactg ctttaaagc gattcatca gagacatac ctgaaccag ttctcacaa acagccaac ggcaatatt tacatcaag tttcattgg tgaagaata aagacgaaa ggataagaa tgtacaaag cagctctcg atcagctcc cgcctattt catacaaaa cggcgaaaa aactcctcg gacatctcc actttattg cgtcacaaa gcaaaaaaa atctgatga atgtcgata atttggctg cagtttggg gaccgagtt taatgcatc aagaagaaa aggataata attcggttg gaaacacca aacacgttc aaaacactg tagttgtac gacagttgt tggaattat atagggata tttttccag aggatcccg ccgaaattg aaaaggaaa aagtcatgt tacaagtgt tggaaaagc acactaagg cactacaag gtgcagcta ataatgcaa aaaaagctg gcgattttc gggtgtgga tttatttgc ataataagg aaggacagc caatgaatg ttgcaattg gcccaaata agactgcgt atgaagttt gcgtggagc taagtccga aaatcaata tgccggtac acgaattgg ttttggagg aagttgttt tgaacgata gattacaac gacctgtgc atttttccg aaaaggttt tggatatcg tccttcggt ggagttatt gggaggaat ccgatcgaa aagacaatc atttagtcc ttcttccta tagcaaaat attccgact ttattcacg acaaatcga caccagttt cagcagaac tcaaatttt ccgataaca gatcaagga atttcaagt tgtacacgt ttgaatttt cccaagcca agcttaact gttttaagg ataaaacgt gtgaggaga aactcttca cttacaaca ttgaagaca tcatgtggt atttaggtc acgaatcta aaaaaaatc cacaatcgc ggtggacaa tcacattta tcgaaacta aaatgcatc cacaacgta ctaaaaact gtccttatt tcggtaatg tgttggttt ggggtgatc cacaaacca gagccaaat ggttgggtg caatgttga aagcgagat atagggaga gtttgatac agccagcta tgcatatca atttaatgg ttaattgag ttaccaaat ttacttatt ttttactta aaacgcacc tttttatgt acgtagcta ataatgatt tctgtgtat ttcttttat ttgcgtgac agtaagtgg caaaatttt aacggttaa aaaggcgaa acgatttta tttatgcgg ctgtgaagt tttacaaaa gttttaaca aaaaatttc cgtaatttt atttattgc attttatta tgactaccg aatttacaa tctgtgatt atgtggaaa agcaattac agaaaccag tgttgcgta aaattgtcg tcattttaa ttccttgta atgatttaa tgaggagaa ataatatta aagtcttcc aaaaaagcc agcaaaact cgtttcaga ttgttatga gcacgtgag ttcaacact tgattgctt ataagaata tgaaactga cgtttttag gttttttat ctgcaatat acattatac ataatcgct ttgattttt tttattcta taagtagtt aattttttg gtgctcaag ccaaacgta tcgtataat tttaattta gattttgca ataattgtg tacaaactt tgtagccat tcctgttcg tagtcttgc cattgtgcc aagtctgcc aactttaat aaaaaaagc acacacaat tgtgatcat gtaaaacat ccactaatg tgaaaaatc gatat
