Status
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Gene information
General information
TC001376 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 15175891..15178794
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001376
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_07918

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Adult morphology
    abdominal odoriferous glands content fragmented - Number of animals showing the phenotype: 1 (13 dpi)
    Days post injection



    abdominal odoriferous glands potentially content not present - Number of animals showing the phenotype: 3 (13 dpi)
    Days post injection

    Comment: Stink glands were not visible

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001376 RA
      MNGTVWQFG VIQTGCKIC EKRAINSFE TRLVLTFEE 
      KNTSLYLLV YHPEGVKKS KEGKYVIFC LTNSGKYEV 
      KERAKISQV YENFDLSLL VYKVKLGKF LGEYWCKGY 
      TPSLSVLNS NKVIAFRKK EGNEYSLRF TTLVTSDLN 
      KFISKTLQK LKTNLGSEI RLMEIFNYN SHSNFLDVL 
      VHVTTTQKR EIEPEFRDL WRKICNTSV DVKFFRNCE 
      FCLSEKKIN LTWPLTKRN NAIVPEEFC LLEDSIPLT 
      RRCNGDFLY GAVWEKTPV QKCAKTEPE SPTTKKLKE 
      FAKNSSSVQ NLWNIVKNA TLGVLDIYY LDLILKKIR 
      QNSVLAFNI VDKLMATDR HILQKSQHI LNLTDATLE 
      LVDEILASE SFNPLEVQL YRKNNLLVH ISNPFLTNI 
      SGILLYNNR QVVNFQRGV NFTAMEERD LDLGVHLPE 
      QLLEQIRNE TQNVSEVFV IIMVFFNDS LFLDDDFYH 
      SDSFVISVT IPGHGAYLQ TPITIAFRP KITPELPPC 
      CGFWDIGKS SNWKKGQWS EIGGQFSGK LKDLQVCSY 
      SHLTHFALL LMSDKTHLD EDDVIIANE DYNDYILEI 
      ITIIGCSLS LFGIFNVFL TAMCYKQWY HKTGTKILL 
      NLCLAILLE SICIQLSEI KPLKTHQNL CKIIGMILH 
      YVVLSKFTW MLIYALLQY YRFVKVIGV IPQKLVLKS 
      LILGWGVPL VLVFLVGLT NDQSYVSES FELCYPRGL 
      LLCFGVLLP ICVIVVANI SIFCVIMYN VSNTVAGNR 
      KLLKPRLYL ALLLFSVLG IPWVFGLVA ELLNGSVLK 
      IVLFYVFCV TATLQGFVL FLFYVVLNR ETRYFWVTK 
      LIKK
    CDS sequence
    • >TC001376 RA
      atgaatgga acggtttgg cagtttgga gtgattcaa 
      acagggtgc aaaatttgc gaaaaacgg gccattaac 
      tcgtttgaa acgagactt gttttgact tttgaggaa 
      aaaaacacc agtctttat ttactagtt taccacccc 
      gaaggggtc aaaaaatcc aaagagggc aaatacgtg 
      attttttgc ctcactaat tctgggaaa tatgaagtg 
      aaagagcga gcaaaaatt agtcaagtg tacgaaaat 
      ttcgatctc tcgttactt gtgtataag gtaaagttg 
      gggaagttt ttgggggag tattggtgc aagggttac 
      accccaagt ctgtcagtt ttaaactcg aataaagtc 
      attgcgttt cggaaaaaa gaaggtaac gaatattca 
      ctcagattc acgactttg gtcacgtct gacttgaac 
      aaattcatt tcgaaaaca ctccagaaa ctaaaaacg 
      aacttgggt tctgagatt cgtttgatg gagattttc 
      aattacaac agtcactct aactttctt gatgtccta 
      gtccatgtc acaacaacc caaaaacgt gaaatcgaa 
      ccagaattt cgtgactta tggcgaaaa atatgcaac 
      acaagtgtt gacgttaaa ttcttccga aattgtgaa 
      ttctgtcta agtgaaaaa aaaatcaat ctcacttgg 
      cccctcacc aaacgtaac aatgccata gtcccagag 
      gaattttgc cttctggag gactccata cctttgact 
      cgacgttgc aacggtgat ttcctctat ggagcagtg 
      tgggaaaaa accccagtc cagaaatgc gcaaaaacc 
      gaacccgaa tccccaaca accaagaaa ttgaaagaa 
      ttcgccaaa aatagctct tcggtgcaa aatctctgg 
      aacattgtc aagaacgca acacttggg gttttggac 
      atttattac ctggatttg attttgaaa aaaatccgc 
      caaaacagc gtcttggct ttcaacatt gtggataaa 
      ctaatggca accgatcgc cacattttg caaaaatcg 
      caacacatt ttaaatttg actgatgcc actttggag 
      ctagttgac gaaattctg gcaagtgag agtttcaac 
      cctctggaa gtgcaactg taccggaag aacaacctc 
      ctagtgcac atttcaaac ccgtttttg accaatatt 
      tcgggaatt ttattgtac aataaccgc caagttgtg 
      aatttccaa cgtggggta aacttcacg gccatggag 
      gagagggac ttggacctg ggggtacac ctcccggag 
      caactcctg gagcagatc cgcaacgaa acgcaaaat 
      gtgtccgaa gttttcgtc ataattatg gtttttttc 
      aacgatagt ttattcctg gatgatgat ttttatcat 
      tcggacagc ttcgtcatc agtgtgact attccaggt 
      catggtgcc tatttacaa actcctatc actatagcg 
      tttcgccct aaaataacc cccgaattg ccgccttgt 
      tgtggtttt tgggacatt ggtaaaagt tctaattgg 
      aaaaaagga caatggtcc gaaattgga ggccaattt 
      tctggcaaa ttgaaagat ttgcaagtt tgttcctac 
      tcacatttg actcacttt gccctactt ttaatgagc 
      gacaagacg caccttgac gaagacgac gtcattatt 
      gctaatgaa gattacaac gattatatt ttagaaatt 
      ataacaata attggatgc tcgttatca ctttttggc 
      atttttaat gttttcctc acagcaatg tgctacaaa 
      cagtggtac cataaaact ggcactaaa attttacta 
      aatttatgt ttggcaatc ctacttgaa tcaatctgt 
      atccaatta tccgaaatc aaaccatta aaaacgcac 
      caaaacttg tgcaaaata attggaatg attctacat 
      tatgtcgta ttgagcaaa ttcacctgg atgttaatt 
      tatgcattg ttacaatac taccgcttc gttaaagtg 
      attggagtc attccgcag aaattggtc ctaaaatcg 
      ttgattctg ggttggggg gttccccta gttttggtg 
      tttctggtc ggtttaaca aatgatcaa agttacgtt 
      tcggaatcg tttgaattg tgttatcct cgcggtttg 
      ttgctttgt ttcggtgtt ttgctcccg atttgtgtg 
      atagttgtg gcaaatatt tcgattttt tgtgtcatt 
      atgtacaat gttagtaac acggttgct ggtaatcgc 
      aaattgttg aaaccgcgg ttgtattta gctttgttg 
      ttgttttcg gttcttggg attccctgg gtttttggg 
      cttgttgcc gagcttttg aatggttca gttttgaaa 
      atcgttctt ttttacgtg ttttgtgtc actgcgact 
      ttgcaaggt tttgtgcta tttttattt tatgttgtt 
      ttgaatagg gagactcgt tatttttgg gtgacaaag 
      ttaataaaa aaataa
    mRNA sequence
    • >TC001376 RA
      tatgaccac aaaataatc acaagaatt tgtatcaat 
      gggctatgg caccctcca aacccaccc ttgtgccac 
      caaaacaat tcaataatt ttccataaa aacgcacca 
      ctgtataat tgtcccaaa aactgtcgt tcggtcaat 
      ccatttaag tgtttttgc aaatcaaca acttgtgat 
      aatttggcg gttgttggc acccttcag gaaaagaaa 
      actgtggag ttgttggta ggggatgaa gtgtgtcaa 
      ttgggccaa gtcgtccct ggaattccc tcatttatg 
      aatggaacg gtttggcag tttggagtg attcaaaca 
      gggtgcaaa atttgcgaa aaacgggcc attaactcg 
      tttgaaacg agacttgtt ttgactttt gaggaaaaa 
      aacaccagt ctttattta ctagtttac caccccgaa 
      ggggtcaaa aaatccaaa gagggcaaa tacgtgatt 
      ttttgcctc actaattct gggaaatat gaagtgaaa 
      gagcgagca aaaattagt caagtgtac gaaaatttc 
      gatctctcg ttacttgtg tataaggta aagttgggg 
      aagtttttg ggggagtat tggtgcaag ggttacacc 
      ccaagtctg tcagtttta aactcgaat aaagtcatt 
      gcgtttcgg aaaaaagaa ggtaacgaa tattcactc 
      agattcacg actttggtc acgtctgac ttgaacaaa 
      ttcatttcg aaaacactc cagaaacta aaaacgaac 
      ttgggttct gagattcgt ttgatggag attttcaat 
      tacaacagt cactctaac tttcttgat gtcctagtc 
      catgtcaca acaacccaa aaacgtgaa atcgaacca 
      gaatttcgt gacttatgg cgaaaaata tgcaacaca 
      agtgttgac gttaaattc ttccgaaat tgtgaattc 
      tgtctaagt gaaaaaaaa atcaatctc acttggccc 
      ctcaccaaa cgtaacaat gccatagtc ccagaggaa 
      ttttgcctt ctggaggac tccatacct ttgactcga 
      cgttgcaac ggtgatttc ctctatgga gcagtgtgg 
      gaaaaaacc ccagtccag aaatgcgca aaaaccgaa 
      cccgaatcc ccaacaacc aagaaattg aaagaattc 
      gccaaaaat agctcttcg gtgcaaaat ctctggaac 
      attgtcaag aacgcaaca cttggggtt ttggacatt 
      tattacctg gatttgatt ttgaaaaaa atccgccaa 
      aacagcgtc ttggctttc aacattgtg gataaacta 
      atggcaacc gatcgccac attttgcaa aaatcgcaa 
      cacatttta aatttgact gatgccact ttggagcta 
      gttgacgaa attctggca agtgagagt ttcaaccct 
      ctggaagtg caactgtac cggaagaac aacctccta 
      gtgcacatt tcaaacccg tttttgacc aatatttcg 
      ggaatttta ttgtacaat aaccgccaa gttgtgaat 
      ttccaacgt ggggtaaac ttcacggcc atggaggag 
      agggacttg gacctgggg gtacacctc ccggagcaa 
      ctcctggag cagatccgc aacgaaacg caaaatgtg 
      tccgaagtt ttcgtcata attatggtt tttttcaac 
      gatagttta ttcctggat gatgatttt tatcattcg 
      gacagcttc gtcatcagt gtgactatt ccaggtcat 
      ggtgcctat ttacaaact cctatcact atagcgttt 
      cgccctaaa ataaccccc gaattgccg ccttgttgt 
      ggtttttgg gacattggt aaaagttct aattggaaa 
      aaaggacaa tggtccgaa attggaggc caattttct 
      ggcaaattg aaagatttg caagtttgt tcctactca 
      catttgact cactttgcc ctactttta atgagcgac 
      aagacgcac cttgacgaa gacgacgtc attattgct 
      aatgaagat tacaacgat tatatttta gaaattata 
      acaataatt ggatgctcg ttatcactt tttggcatt 
      tttaatgtt ttcctcaca gcaatgtgc tacaaacag 
      tggtaccat aaaactggc actaaaatt ttactaaat 
      ttatgtttg gcaatccta cttgaatca atctgtatc 
      caattatcc gaaatcaaa ccattaaaa acgcaccaa 
      aacttgtgc aaaataatt ggaatgatt ctacattat 
      gtcgtattg agcaaattc acctggatg ttaatttat 
      gcattgtta caatactac cgcttcgtt aaagtgatt 
      ggagtcatt ccgcagaaa ttggtccta aaatcgttg 
      attctgggt tggggggtt cccctagtt ttggtgttt 
      ctggtcggt ttaacaaat gatcaaagt tacgtttcg 
      gaatcgttt gaattgtgt tatcctcgc ggtttgttg 
      ctttgtttc ggtgttttg ctcccgatt tgtgtgata 
      gttgtggca aatatttcg attttttgt gtcattatg 
      tacaatgtt agtaacacg gttgctggt aatcgcaaa 
      ttgttgaaa ccgcggttg tatttagct ttgttgttg 
      ttttcggtt cttgggatt ccctgggtt tttgggctt 
      gttgccgag cttttgaat ggttcagtt ttgaaaatc 
      gttcttttt tacgtgttt tgtgtcact gcgactttg 
      caaggtttt gtgctattt ttattttat gttgttttg 
      aatagggag actcgttat ttttgggtg acaaagtta 
      ataaaaaaa taaaattga cacttactt gttttaatt 
      tgttaaaac caagagctc caacacata tgtccctct 
      tgctgcact ataagtgtt gttttgcat tcctttaaa 
      attgattaa atgatataa aagtct
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY