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Gene information
General information
TC001349 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 14928013..14929626
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC001349
    iBeetle screen iB_07916

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001349 RA
      MLEANKKIF GSISQQFPK LETFVFFDG SIRHTQVCQ 
PNGALRDKC LLTNYSQYM HKFRSTCSE AISSCFYNN 
EEFPCCDEF LPIDTYHGP CFGFNSLVS GKYTSREDL 
PFKITRDSN FGYGNLSIE LYHDGFIVY GLSQDSVPT 
KESSSEFVL LDSRTSSGI VAKTAYKAF LSVKETFTD 
EAARLMKIA NRGCRFYDE NNLQHAKLY STSACVNEC 
LVEAQREIC NCTYHILPH NDQVKDCDF EGLLCTIDN 
KKVKEKTTS CSCTSSCSD VEVVNIGFT KTPIHSSQF 
RNDKSQFQL VVLLVCLWV QVCSQWSRL FIIFSCVRL 
ATNT
    CDS sequence
    • >TC001349 RA
      atgcttgaa gctaacaag aaaattttc ggttccata 
tctcaacag ttccccaaa ctcgaaacc ttcgttttc 
ttcgacggt tccataaga cacacgcaa gtttgccaa 
ccaaatggt gctctacgt gacaaatgc ttgctaaca 
aattactcg caatacatg cataagttc cgcagcact 
tgttcggaa gcaattagt tcgtgcttt tacaacaac 
gaagaattt ccctgctgt gacgaattc ttgcccatt 
gacacttac cacgggcct tgctttggc ttcaattct 
ctggtttca ggaaaatac acaagtcgg gaagacctc 
cctttcaaa ataacgcgc gactcaaat ttcggttac 
ggcaactta tcaattgag ctgtaccat gatgggttc 
attgtgtat gggttgagc caagactcc gtccctact 
aaggaaagt tccagcgag tttgtgttg ctagattca 
cgcacaagc tcgggaatt gttgctaaa acagcctac 
aaggcgttt ttatcagtt aaagaaact ttcacggac 
gaagccgcc aggttgatg aaaatcgca aatcgtggt 
tgtcgattt tacgatgaa aataacttg caacatgca 
aaactgtac agtacttca gcttgcgtg aacgaatgt 
cttgttgaa gcccaaagg gagatttgc aattgcact 
tatcacatt ttaccacac aatgatcaa gtcaaagac 
tgtgatttt gaggggctt ttgtgcaca attgataac 
aagaaagtt aaggagaaa acaacttct tgcagttgt 
acgagttct tgctctgac gttgaagtt gtcaatatt 
ggctttacc aaaactcca atacactcg agtcagttc 
cgaaatgac aaaagccag tttcagttg gtggttctg 
ttggtttgt ttgtgggtg caagtttgc tcacagtgg 
tcgagatta tttattatt ttttcgtgc gtccgcttg 
gcgacaaat acataa
    mRNA sequence
    • >TC001349 RA
      actggactg aaagatttt tttggacaa tatgtgtcc 
taacttctt gggccgcat cgattatgc taatgttta 
acgcactag accagtatc aaaaccaaa aaatatcct 
tttcagtgg acacatctt acttaaatt ggcaaacaa 
actttcctt caatcgtaa tttgcgaac gatcgaatc 
caaaaaaaa tgcttgaag ctaacaaga aaattttcg 
gttccatat ctcaacagt tccccaaac tcgaaacct 
tcgttttct tcgacggtt ccataagac acacgcaag 
tttgccaac caaatggtg ctctacgtg acaaatgct 
tgctaacaa attactcgc aatacatgc ataagttcc 
gcagcactt gttcggaag caattagtt cgtgctttt 
acaacaacg aagaatttc cctgctgtg acgaattct 
tgcccattg acacttacc acgggcctt gctttggct 
tcaattctc tggtttcag gaaaataca caagtcggg 
aagacctcc ctttcaaaa taacgcgcg actcaaatt 
tcggttacg gcaacttat caattgagc tgtaccatg 
atgggttca ttgtgtatg ggttgagcc aagactccg 
tccctacta aggaaagtt ccagcgagt ttgtgttgc 
tagattcac gcacaagct cgggaattg ttgctaaaa 
cagcctaca aggcgtttt tatcagtta aagaaactt 
tcacggacg aagccgcca ggttgatga aaatcgcaa 
atcgtggtt gtcgatttt acgatgaaa ataacttgc 
aacatgcaa aactgtaca gtacttcag cttgcgtga 
acgaatgtc ttgttgaag cccaaaggg agatttgca 
attgcactt atcacattt taccacaca atgatcaag 
tcaaagact gtgattttg aggggcttt tgtgcacaa 
ttgataaca agaaagtta aggagaaaa caacttctt 
gcagttgta cgagttctt gctctgacg ttgaagttg 
tcaatattg gctttacca aaactccaa tacactcga 
gtcagttcc gaaatgaca aaagccagt ttcagttgg 
tggttctgt tggtttgtt tgtgggtgc aagtttgct 
cacagtggt cgagattat ttattattt tttcgtgcg 
tccgcttgg cgacaaata cataaaaca tgaaaggaa 
caaagaaat aaaaaaatc agagattg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY