Status
Please wait ...
Gene information
General information
TC000202 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 14821201..14822983
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000202
    iBeetle screen iB_07915

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Adult morphology
    abdominal odoriferous glands partially content lighter - Number of animals showing the phenotype: 4 (13 dpi)
    Days post injection



    abdominal odoriferous glands content fragmented - Number of animals showing the phenotype: 4 (13 dpi)
    Days post injection

    Comment: content looks compartimentet

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000202 RA
      MCRKYMHGS GLGPTSPKI KRFISLWLL FPVSLLLDV 
      LVIYDFHFL DNDIFKTAE LLESVSSFG QLPIRKFIL 
      TYHSKLIQN LLEDRKKFW SYEMFGETY GKFLRRKMV 
      LATRLIQTM IFFGASVAT LMFVSTLAD DRKTVPLEC 
      WIPEFKHST HVVLVMQFC SLCEIIYLV GAVDCLYVL 
      TCVDIKIQF LLLQKKLKT IQVGVKPME ECLNELTIC 
      VKHHNLLLR SHKSLNRIF SEYFFVQYF VSVLAACVQ 
      LYILMYITA SLEDIMKSI VYLSAVVFQ VAIFFMPAS 
      DIEEEAEQF AVEIYNVNW ECTSGTKFR KQLLFMLMK 
      AQKPLYMLG GGMIHANRN EYIVLFRLA FSISTLLGG 
      MNENGRTDK
    CDS sequence
    • >TC000202 RA
      atgtgtcga aaatatatg cacgggagc ggcttagga 
      cccacaagt ccaaaaatt aagcggttt atctcccta 
      tggttgttg tttcctgta agcttgctt ttggacgtt 
      ttggtgatt tatgatttt cattttctg gataatgat 
      attttcaaa acagcagag ttacttgaa tcggtctcc 
      tcttttgga caactccca attcgtaaa ttcattcta 
      acttatcat tcaaaatta atccaaaat ctgctcgag 
      gatcgaaaa aaattttgg tcgtacgaa atgtttggt 
      gaaacatat ggaaagttt cttcgccgc aaaatggtc 
      ctagctacg agactaatc caaacgatg atttttttt 
      ggggcttct gttgccacg ttaatgttt gtttcgact 
      ttagccgac gaccggaaa acagttcct ttggaatgt 
      tggattcct gagtttaaa cactcgact catgtggta 
      cttgtaatg cagttttgt tccttgtgt gagattatt 
      tatctcgtt ggtgccgtt gactgtttg tacgtttta 
      acgtgcgtt gatattaaa atacaattt ctgcttttg 
      caaaagaaa ctgaaaacg attcaagtt ggagttaaa 
      cccatggaa gagtgtttg aacgaattg accatttgc 
      gtgaaacat cacaatttg ctgttgagg agtcacaag 
      agtctgaac cggattttt tccgaatac ttttttgtc 
      cagtacttt gtgagcgtt ctagctgct tgtgtccaa 
      ctttacatc ctgatgtac ataactgcg agtctcgag 
      gatattatg aagagcatt gtttacttg tctgctgta 
      gttttccaa gttgcgatt tttttcatg cctgcgagc 
      gacatcgag gaagaggct gaacaattt gctgttgaa 
      atttataac gtcaattgg gaatgtacc agtggaaca 
      aaattccgt aaacaactc ttgtttatg ttgatgaag 
      gcccaaaaa cccttatac atgctgggc ggcggaatg 
      attcatgcc aaccggaac gaatatatt gtgttattc 
      agactcgct ttctccatt tctacgtta ttgggagga 
      atgaacgaa aatggaagg accgataaa taa
    mRNA sequence
    • >TC000202 RA
      tacgggcaa atggctcct aataagagt agtaagaaa 
      gactttctc gacatgtgt cgaaaatat atgcacggg 
      agcggctta ggacccaca agtccaaaa attaagcgg 
      tttatctcc ctatggttg ttgtttcct gtaagcttg 
      cttttggac gttttggtg atttatgat tttcatttt 
      ctggataat gatattttc aaaacagca gagttactt 
      gaatcggtc tcctctttt ggacaactc ccaattcgt 
      aaattcatt ctaacttat cattcaaaa ttaatccaa 
      aatctgctc gaggatcga aaaaaattt tggtcgtac 
      gaaatgttt ggtgaaaca tatggaaag tttcttcgc 
      cgcaaaatg gtcctagct acgagacta atccaaacg 
      atgattttt tttggggct tctgttgcc acgttaatg 
      tttgtttcg actttagcc gacgaccgg aaaacagtt 
      cctttggaa tgttggatt cctgagttt aaacactcg 
      actcatgtg gtacttgta atgcagttt tgttccttg 
      tgtgagatt atttatctc gttggtgcc gttgactgt 
      ttgtacgtt ttaacgtgc gttgatatt aaaatacaa 
      tttctgctt ttgcaaaag aaactgaaa acgattcaa 
      gttggagtt aaacccatg gaagagtgt ttgaacgaa 
      ttgaccatt tgcgtgaaa catcacaat ttgctgttg 
      aggagtcac aagagtctg aaccggatt ttttccgaa 
      tactttttt gtccagtac tttgtgagc gttctagct 
      gcttgtgtc caactttac atcctgatg tacataact 
      gcgagtctc gaggatatt atgaagagc attgtttac 
      ttgtctgct gtagttttc caagttgcg atttttttc 
      atgcctgcg agcgacatc gaggaagag gctgaacaa 
      tttgctgtt gaaatttat aacgtcaat tgggaatgt 
      accagtgga acaaaattc cgtaaacaa ctcttgttt 
      atgttgatg aaggcccaa aaaccctta tacatgctg 
      ggcggcgga atgattcat gccaaccgg aacgaatat 
      attgtgtta ttcagactc gctttctcc atttctacg 
      ttattggga ggaatgaac gaaaatgga aggaccgat 
      aaataatgc aagaggcga cactaaatt cgacattct 
      tttattaca ccacacgaa aatacagtt tacataagc 
      tt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY