Status
Please wait ...
Gene information
General information
TC001276 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 13690669..13691741
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001276
    iBeetle screen iB_07903

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001276 RA
      MSRRRGRDY DHVDYTKLA PADKDNGFI 
      DAQFITPAQ KVPWKAICL ATMLLVVGT 
      FLLIFGSLV VSGHIILNY SDRMWPMII 
      LGLLMFIPG AYHVRIAYY AYKQVPGYS 
      FDDIPEFD
    • >TC001276 RB
      MNFKSLSPL TTEFDNGRT STSQISLLP 
      TMSLHSITE YLDDLKMSR RRGRDYDHV 
      DYTKLAPAD KDNGFIDAQ FITPAQKVP 
      WKAICLATM LLVVGTFLL IFGSLVVSG 
      HIILNYSDR MWPMIILGL LMFIPGAYH 
      VRIAYYAYK QVPGYSFDD IPEFD
    • >TC001276 RC
      MSLHSITEY LDDLKMSRR RGRDYDHVD 
      YTKLAPADK DNGFIDAQF ITPAQKVPW 
      KAICLATML LVVGTFLLI FGSLVVSGH 
      IILNYSDRM WPMIILGLL MFIPGAYHV 
      RIAYYAYKQ VPGYSFDDI PEFD
    CDS sequence
    • >TC001276 RA
      atgtccaga aggcggggt cgcgattac gaccacgtt 
      gattacaca aagctagca cctgctgat aaggacaat 
      ggctttatt gatgcacag ttcattaca cctgcccag 
      aaagtacca tggaaggcc atttgctta gccactatg 
      ttgctagtc gtgggcacg tttttgctc atttttggg 
      tcgctggtg gtcagtggg cacattatc ctgaactat 
      tcagacaga atgtggcca atgatcatc ctggggctg 
      ctgatgttc atcccgggg gcctaccat gtacgcatc 
      gcctactat gcctacaag caagtgcct ggctactct 
      tttgacgac atccccgag tttgattag
    • >TC001276 RB
      atgaatttt aaatctcta agccccctg acgaccgaa 
      ttcgataat ggtcgtaca agtaccagt cagatttct 
      ctattaccg accatgagt ttacattcc attacagaa 
      tatttggac gaccttaaa atgtccaga aggcggggt 
      cgcgattac gaccacgtt gattacaca aagctagca 
      cctgctgat aaggacaat ggctttatt gatgcacag 
      ttcattaca cctgcccag aaagtacca tggaaggcc 
      atttgctta gccactatg ttgctagtc gtgggcacg 
      tttttgctc atttttggg tcgctggtg gtcagtggg 
      cacattatc ctgaactat tcagacaga atgtggcca 
      atgatcatc ctggggctg ctgatgttc atcccgggg 
      gcctaccat gtacgcatc gcctactat gcctacaag 
      caagtgcct ggctactct tttgacgac atccccgag 
      tttgattag
    • >TC001276 RC
      atgagttta cattccatt acagaatat ttggacgac 
      cttaaaatg tccagaagg cggggtcgc gattacgac 
      cacgttgat tacacaaag ctagcacct gctgataag 
      gacaatggc tttattgat gcacagttc attacacct 
      gcccagaaa gtaccatgg aaggccatt tgcttagcc 
      actatgttg ctagtcgtg ggcacgttt ttgctcatt 
      tttgggtcg ctggtggtc agtgggcac attatcctg 
      aactattca gacagaatg tggccaatg atcatcctg 
      gggctgctg atgttcatc ccgggggcc taccatgta 
      cgcatcgcc tactatgcc tacaagcaa gtgcctggc 
      tactctttt gacgacatc cccgagttt gattag
    mRNA sequence
    • >TC001276 RA
      ttatgtaaa atgaaactt ctaaaataa cgtgtcagg 
      cggcaattt ttgttgtta cgtgcagtt gagtaattt 
      cgccccaaa tttgtctaa atcgagtgt taattttaa 
      aatttcaag gagaacgca tagtgagtc caaacgcaa 
      caaaatagc acccaaaaa aaaataaaa aagacagtt 
      tggggcgca aaaaggggc aaatgtttt ggatattaa 
      ttaaataaa gggagtgtt cattattga tccccgtca 
      tgaatttta aatctctaa gccccctga cgaccgaat 
      tcgataatg gtcgtacaa gtaccagtc agatttctc 
      tattaccga ccatgagtt tacattcca ttacaggtt 
      aactcaact tccctataa ttcgcctta tcagagggt 
      aaatacact aaaaatcaa cactctttc tttgcagaa 
      tatttggac gaccttaaa atgtccaga aggcggggt 
      cgcgattac gaccacgtt gattacaca aagctagca 
      cctgctgat aaggacaat ggctttatt gatgcacag 
      ttcattaca cctgcccag aaagtacca tggaaggcc 
      atttgctta gccactatg ttgctagtc gtgggcacg 
      tttttgctc atttttggg tcgctggtg gtcagtggg 
      cacattatc ctgaactat tcagacaga atgtggcca 
      atgatcatc ctggggctg ctgatgttc atcccgggg 
      gcctaccat gtacgcatc gcctactat gcctacaag 
      caagtgcct ggctactct tttgacgac atccccgag 
      tttgattag gggggcttt tctaattgt agggagaac 
      agtattgag agaatagat tcgtgtacc tgagttaat 
      ttttagata aggactaag caatcagct ttgcgatta 
      atgtgcctt ctcttgtcg ttaattaat taatgtatg 
      tgcccttga tcactgtat actttgtga ttttttttg 
      ttaacgaat acattactt aaatatttg gaataaatg 
      tttatctgt tta
    • >TC001276 RB
      ttatgtaaa atgaaactt ctaaaataa cgtgtcagg 
      cggcaattt ttgttgtta cgtgcagtt gagtaattt 
      cgccccaaa tttgtctaa atcgagtgt taattttaa 
      aatttcaag gagaacgca tagtgagtc caaacgcaa 
      caaaatagc acccaaaaa aaaataaaa aagacagtt 
      tggggcgca aaaaggggc aaatgtttt ggatattaa 
      ttaaataaa gggagtgtt cattattga tccccgtca 
      tgaatttta aatctctaa gccccctga cgaccgaat 
      tcgataatg gtcgtacaa gtaccagtc agatttctc 
      tattaccga ccatgagtt tacattcca ttacagaat 
      atttggacg accttaaaa tgtccagaa ggcggggtc 
      gcgattacg accacgttg attacacaa agctagcac 
      ctgctgata aggacaatg gctttattg atgcacagt 
      tcattacac ctgcccaga aagtaccat ggaaggcca 
      tttgcttag ccactatgt tgctagtcg tgggcacgt 
      ttttgctca tttttgggt cgctggtgg tcagtgggc 
      acattatcc tgaactatt cagacagaa tgtggccaa 
      tgatcatcc tggggctgc tgatgttca tcccggggg 
      cctaccatg tacgcatcg cctactatg cctacaagc 
      aagtgcctg gctactctt ttgacgaca tccccgagt 
      ttgattagg ggggctttt ctaattgta gggagaaca 
      gtattgaga gaatagatt cgtgtacct gagttaatt 
      tttagataa ggactaagc aatcagctt tgcgattaa 
      tgtgccttc tcttgtcgt taattaatt aatgtatgt 
      gcccttgat cactgtata ctttgtgat tttttttgt 
      taacgaata cattactta aatatttgg aataaatgt 
      ttatctgtt ta
    • >TC001276 RC
      ttatgtaaa atgaaactt ctaaaataa cgtgtcagg 
      cggcaattt ttgttgtta cgtgcagtt gagtaattt 
      cgccccaaa tttgtctaa atcgagtgt taattttaa 
      aatttcaag gagaacgca tagtgagtc caaacgcaa 
      caaaatagc acccaaaaa aaaataaaa aagacagtt 
      tggggcgca aaaaggggc aaatgtttt ggatgtatg 
      ttaaaggta cgtcgttag caccagcta aaccccttt 
      ctattattt tagattaat taaataaag ggagtgttc 
      attattgat ccccgtcat gaattttaa atctctaag 
      ccccctgac gaccgaatt cgataatgg tcgtacaag 
      taccagtca gatttctct attaccgac catgagttt 
      acattccat tacagaata tttggacga ccttaaaat 
      gtccagaag gcggggtcg cgattacga ccacgttga 
      ttacacaaa gctagcacc tgctgataa ggacaatgg 
      ctttattga tgcacagtt cattacacc tgcccagaa 
      agtaccatg gaaggccat ttgcttagc cactatgtt 
      gctagtcgt gggcacgtt tttgctcat ttttgggtc 
      gctggtggt cagtgggca cattatcct gaactattc 
      agacagaat gtggccaat gatcatcct ggggctgct 
      gatgttcat cccgggggc ctaccatgt acgcatcgc 
      ctactatgc ctacaagca agtgcctgg ctactcttt 
      tgacgacat ccccgagtt tgattaggg gggcttttc 
      taattgtag ggagaacag tattgagag aatagattc 
      gtgtacctg agttaattt ttagataag gactaagca 
      atcagcttt gcgattaat gtgccttct cttgtcgtt 
      aattaatta atgtatgtg cccttgatc actgtatac 
      tttgtgatt ttttttgtt aacgaatac attacttaa 
      atatttgga ataaatgtt tatctgttt a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY