Status
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Gene information
General information
TC001275 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 13670039..13671658
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC001275
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_07902

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    Adult morphology
    abdominal odoriferous glands content decreased - Number of animals showing the phenotype: 4 (13 dpi)
    Days post injection


    abdominal odoriferous glands content fragmented - Number of animals showing the phenotype: 2 (13 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001275 RA
      MVVQKIDLL EPFDNVTRL LKILGLWYS PNETIVYKI 
YKNFVMATC FLYTLTCTV YGFKFMSFE TLEIAFGAV 
EGVLKSLMF RLKFQKIAE SWQQIRQQE FQPRNEHQR 
TVLKWYIEV TKSLFLVYF FGVYIGCIS ALTVSSWLR 
HKDFPTDHW FPFNYRRPF LYQYIYVHI TVGFYLTAF 
LNCASDSCF YLSLLHITA QCEILADTL KNVHDLHKL 
NAAKKNSGQ KGEDEVMNQ ILIECMKHY NLIKKYTSL 
VADCFKEII TLQFVPTIV MICIAMYKI STLEPSNTQ 
FWFFAFTEL GAITQIFIY CFVGNLVTS TSQKLFYAT 
FESQWYNAS QKFKKNLIT VMMAVQRPV IFYGWNIFA 
INYATFKSI VQTSWSMCV AFRSTQDL
    CDS sequence
    • >TC001275 RA
      atggtggtg cagaaaatt gacttactc gaacctttc 
gataatgtc acccgtctg ctcaagatt ttgggtttg 
tggtattcc ccaaacgag acaattgtg tacaaaatt 
tacaaaaat tttgtcatg gcaacgtgc tttttgtac 
actctaaca tgcaccgtt tacggattc aagttcatg 
tcttttgag actttggaa atagcattt ggggccgta 
gaaggagtt ctgaagagt ttaatgttc cgcctaaaa 
ttccaaaag atcgcagag tcgtggcag caaatccgc 
caacaagaa tttcagccc agaaacgaa catcaaaga 
actgtttta aaatggtac attgaagtg acaaaatcg 
cttttccta gtttacttt ttcggagtc tacatcggt 
tgcataagc gctctcact gtgtcgtct tggttgaga 
cacaaagat ttcccaaca gaccactgg tttccattt 
aattatcgc aggccgttc ttgtaccaa tatatttat 
gttcatata acagttggt ttctatctt actgctttc 
ttgaactgt gcctcagac tcttgtttt tacttgtca 
ttgctgcac attacagct cagtgtgaa attcttgcg 
gatactctt aaaaatgtc cacgacttg cacaaacta 
aacgcggcg aaaaaaaat tcgggacag aaaggtgag 
gacgaagtg atgaatcag attctaata gaatgcatg 
aaacattac aacctgatt aaaaaatac accagttta 
gtagcagac tgtttcaaa gaaattata actttgcag 
ttcgttcca acgattgta atgatttgc attgcaatg 
tataaaata tccacgctc gagccgtcg aacacgcag 
ttttggttt tttgcgttt acggaactc ggtgctata 
acccaaatt ttcatttac tgtttcgtt ggcaatctg 
gtcactagt acaagtcaa aaattattc tatgcaacg 
tttgaatcg cagtggtac aacgcgtct caaaaattc 
aagaaaaat ttaattacg gtgatgatg gcagttcag 
cgtcctgtt attttttat ggttggaat atttttgca 
atcaattat gcaactttc aaatcgatt gtccaaact 
tcatggagc atgtgtgtc gcttttaga agtacacaa 
gatctgtaa
    mRNA sequence
    • >TC001275 RA
      ccactttgt caaaaatgg tggtgcaga aaattgact 
tactcgaac ctttcgata atgtcaccc gtctgctca 
agattttgg gtttgtggt attccccaa acgagacaa 
ttgtgtaca aaatttaca aaaattttg tcatggcaa 
cgtgctttt tgtacactc taacatgca ccgtttacg 
gattcaagt tcatgtctt ttgagactt tggaaatag 
catttgggg ccgtagaag gagttctga agagtttaa 
tgttccgcc taaaattcc aaaagatcg cagagtcgt 
ggcagcaaa tccgccaac aagaatttc agcccagaa 
acgaacatc aaagaactg ttttaaaat ggtacattg 
aagtgacaa aatcgcttt tcctagttt actttttcg 
gagtctaca tcggttgca taagcgctc tcactgtgt 
cgtcttggt tgagacaca aagatttcc caacagacc 
actggtttc catttaatt atcgcaggc cgttcttgt 
accaatata tttatgttc atataacag ttggtttct 
atcttactg ctttcttga actgtgcct cagactctt 
gtttttact tgtcattgc tgcacatta cagctcagt 
gtgaaattc ttgcggata ctcttaaaa atgtccacg 
acttgcaca aactaaacg cggcgaaaa aaaattcgg 
gacagaaag gtgaggacg aagtgatga atcagattc 
taatagaat gcatgaaac attacaacc tgattaaaa 
aatacacca gtttagtag cagactgtt tcaaagaaa 
ttataactt tgcagttcg ttccaacga ttgtaatga 
tttgcattg caatgtata aaatatcca cgctcgagc 
cgtcgaaca cgcagtttt ggttttttg cgtttacgg 
aactcggtg ctataaccc aaattttca tttactgtt 
tcgttggca atctggtca ctagtacaa gtcaaaaat 
tattctatg caacgtttg aatcgcagt ggtacaacg 
cgtctcaaa aattcaaga aaaatttaa ttacggtga 
tgatggcag ttcagcgtc ctgttattt tttatggtt 
ggaatattt ttgcaatca attatgcaa ctttcaaat 
cgattgtcc aaacttcat ggagcatgt gtgtcgctt 
ttagaagta cacaagatc tgtaatctg tttttcagg 
tatcactta ttacacggt ctcactgct ttctacaca 
tattaatat aatttagaa tcaacctgt atatttttg 
agaaaatta aaaaattaa gtctttctt aggattctt 
aggaagttt gaattgtta agttgatgc cacgtttga 
atttcttgg tgtacggaa gagaagcgc ttttagagt 
tgtatattg accagaaag aatggcgat ttattatta 
tattcaata tatttttta ttaacta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY