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Gene information
General information
TC001243 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 13153732..13155696
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001243
    iBeetle screen iB_07900

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Adult morphology
    abdominal odoriferous glands colour lighter - Number of animals showing the phenotype: 4 (13 dpi)
    Days post injection



    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    larva everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    head capsule size decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    head appendages partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001243 RA
      MDVNQRLSS FQEISKTLL SKIFSSVPR TKHIVIEPS 
      VIRPLERVC SVSWLKGNG IEKIFKLEG KCPNFGNSP 
      VFYMIFNQR ATFRRVVDQ IRSQIDQEN PVKNRFHVI 
      IVPHYQRCF DDMLEALGL CSIIKLHCF LWFPVHLDT 
      GILSLELPH IYKKLFLVH DYTALHPLA RALWNMFSV 
      IGLPKFQIA VGEYSNKIL RQIDLLAAE KGETERLNP 
      EVGALIIID RSVDYPSAL LTPGTYSAL LNEVYGVTT 
      GMVECKESE DEVRLNPVV KKQPVKFTL DSNLDSIYS 
      EIKNLYFTE VTSSLRTMT RKLKSEALE SRDMGLNEM 
      RHFVQKLLQ PAQTRKKLI ANHLLAAET IANVLGPRY 
      ETQREIEAN IIQNTNRAS NFSHLEESL CTEDDPCVT 
      LRLFCLLCV TQKLTDSEI KNFWRKFLH QFGFASSSA 
      MRYLINANF VPEAGVSGL PKIRFPKFT TKEFYANAN 
      KFKQIPANP EFADLGHPG CASYVFGGI YIPLVTKIA 
      GMIVSGTPI EEIRSKLEG LGMVTLRSG DAYPLERRT 
      VLIYFVGGV TYAEIAACN LLETFTRAT IVVMSDRIV 
      SGNDLMKQM LS
    CDS sequence
    • >TC001243 RA
      atggatgta aaccaaaga ttatcgtct tttcaagag 
      atttccaag accctgctt tcgaaaatc ttcagttct 
      gtccctcgg acgaaacat atcgttatc gaaccgagt 
      gtgattcgc ccccttgag cgcgtttgt agcgtgagc 
      tggctgaag ggaaacggc attgagaag atatttaaa 
      ctggaaggg aaatgcccg aattttggc aacagcccc 
      gttttttac atgattttc aatcaaagg gctacattc 
      cgacgagtg gttgatcag attcggtca cagatcgac 
      caagagaat ccagttaag aataggttt cacgtaatc 
      attgtccca cactatcag cgttgtttt gacgacatg 
      ttagaagca ctgggcttg tgttccatt atcaagtta 
      cactgcttc ctttggttt cctgtccac ttggacacg 
      gggatttta agtttggaa ttgccccac atttacaag 
      aaactgttt ttggtgcat gactacacg gccttgcac 
      cccttggcc agagcccta tggaacatg ttcagtgtg 
      attggttta ccaaagttc cagatagct gtaggcgaa 
      tattcgaat aaaattttg cgccagatt gacttgctc 
      gccgccgag aagggcgaa accgagcga cttaatccc 
      gaggtgggg gctttaatc ataattgac aggagcgta 
      gattaccca agtgcctta ctaacacct ggtacctat 
      tccgcttta ctaaacgaa gtgtacgga gttactact 
      gggatggtc gagtgcaag gaaagcgag gatgaagtc 
      cgccttaat ccagtcgta aaaaaacag ccggtgaaa 
      ttcaccctc gatagcaat ctcgactca atttactcc 
      gagataaaa aacctgtat ttcacggaa gttacgtca 
      agtttgagg accatgacg cgcaaatta aaatccgag 
      gcgctcgag tcgcgtgac atgggctta aacgaaatg 
      cgccacttc gtgcaaaag ctcctccag cccgcacaa 
      actcgtaaa aaactaatc gccaatcat ttgcttgcc 
      gccgagacc atagccaat gtcctcggc ccgcgctac 
      gaaacgcag cgcgagatc gaggcgaac atcatccag 
      aacacgaac cgcgcctcc aacttctcc cacctggag 
      gagagtctg tgcacggaa gacgacccg tgtgtcaca 
      ctgcggctc ttttgtttg ctctgtgtc acgcagaaa 
      ctcaccgac agcgaaatt aaaaacttc tggaggaag 
      tttctacac cagttcggg ttcgcctcc agttcggct 
      atgcgttat ttgatcaac gctaatttc gtgccggag 
      gcgggggtt tcgggactg ccgaaaata cgcttcccg 
      aagttcaca acgaaggag ttttacgcc aatgcgaat 
      aagtttaaa cagattccg gctaatccg gagtttgcc 
      gatttgggg catccgggg tgtgcgagt tatgtcttt 
      ggggggatt tacataccg ttggttacg aaaatcgcg 
      ggtatgatc gttagtggc actccgatt gaggagatt 
      aggagtaaa ttggagggg cttgggatg gtcacgttg 
      aggagtggt gacgcctat cctttggaa aggcggact 
      gtgctcatt tatttcgtt ggaggggtc acgtatgcc 
      gaaattgca gcttgtaat cttttggag actttcacg 
      agggctaca attgtcgtt atgagcgac aggattgtc 
      tcaggaaac gatttgatg aagcaaatg ctgtcttaa
    mRNA sequence
    • >TC001243 RA
      tgataagtt atttactaa taatggatg taaaccaaa 
      gattatcgt cttttcaag agatttcca agaccctgc 
      tttcgaaaa tcttcagtt ctgtccctc ggacgaaac 
      atatcgtta tcgaaccga gtgtgattc gcccccttg 
      agcgcgttt gtagcgtga gctggctga agggaaacg 
      gcattgaga agatattta aactggaag ggaaatgcc 
      cgaattttg gcaacagcc ccgtttttt acatgattt 
      tcaatcaaa gggctacat tccgacgag tggttgatc 
      agattcggt cacagatcg accaagaga atccagtta 
      agaataggt ttcacgtaa tcattgtcc cacactatc 
      agcgttgtt ttgacgaca tgttagaag cactgggct 
      tgtgttcca ttatcaagt tacactgct tcctttggt 
      ttcctgtcc acttggaca cggggattt taagtttgg 
      aattgcccc acatttaca agaaactgt ttttggtgc 
      atgactaca cggccttgc accccttgg ccagagccc 
      tatggaaca tgttcagtg tgattggtt taccaaagt 
      tccagatag ctgtaggcg aatattcga ataaaattt 
      tgcgccaga ttgacttgc tcgccgccg agaagggcg 
      aaaccgagc gacttaatc ccgaggtgg gggctttaa 
      tcataattg acaggagcg tagattacc caagtgcct 
      tactaacac ctggtacct attccgctt tactaaacg 
      aagtgtacg gagttacta ctgggatgg tcgagtgca 
      aggaaagcg aggatgaag tccgcctta atccagtcg 
      taaaaaaac agccggtga aattcaccc tcgatagca 
      atctcgact caatttact ccgagataa aaaacctgt 
      atttcacgg aagttacgt caagtttga ggaccatga 
      cgcgcaaat taaaatccg aggcgctcg agtcgcgtg 
      acatgggct taaacgaaa tgcgccact tcgtgcaaa 
      agctcctcc agcccgcac aaactcgta aaaaactaa 
      tcgccaatc atttgcttg ccgccgaga ccatagcca 
      atgtcctcg gcccgcgct acgaaacgc agcgcgaga 
      tcgaggcga acatcatcc agaacacga accgcgcct 
      ccaacttct cccacctgg aggagagtc tgtgcacgg 
      aagacgacc cgtgtgtca cactgcggc tcttttgtt 
      tgctctgtg tcacgcaga aactcaccg acagcgaaa 
      ttaaaaact tctggagga agtttctac accagttcg 
      ggttcgcct ccagttcgg ctatgcgtt atttgatca 
      acgctaatt tcgtgccgg aggcggggg tttcgggac 
      tgccgaaaa tacgcttcc cgaagttca caacgaagg 
      agttttacg ccaatgcga ataagttta aacagattc 
      cggctaatc cggagtttg ccgatttgg ggcatccgg 
      ggtgtgcga gttatgtct ttgggggga tttacatac 
      cgttggtta cgaaaatcg cgggtatga tcgttagtg 
      gcactccga ttgaggaga ttaggagta aattggagg 
      ggcttggga tggtcacgt tgaggagtg gtgacgcct 
      atcctttgg aaaggcgga ctgtgctca tttatttcg 
      ttggagggg tcacgtatg ccgaaattg cagcttgta 
      atcttttgg agactttca cgagggcta caattgtcg 
      ttatgagcg acaggattg tctcaggaa acgatttga 
      tgaagcaaa tgctgtctt aaaccattt tgtattatt 
      ttttctaca aaaacagtc aaaaaatca cgtttttgt 
      ttcaataac ttgtacgac tgttttgtt tgccaaatt 
      ctatatttt gtaccgttg tttttatta aaataaacg 
      aaaaaaagt tca
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