General information | |||
TC001243 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 13153732..13155696 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC001243 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_07900 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
remarks: muscle pattern potentially obscured by segmentation defect,
larva everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%
head capsule size decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
head appendages partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC001243 RA
MDVNQRLSS FQEISKTLL SKIFSSVPR TKHIVIEPS VIRPLERVC SVSWLKGNG IEKIFKLEG KCPNFGNSP VFYMIFNQR ATFRRVVDQ IRSQIDQEN PVKNRFHVI IVPHYQRCF DDMLEALGL CSIIKLHCF LWFPVHLDT GILSLELPH IYKKLFLVH DYTALHPLA RALWNMFSV IGLPKFQIA VGEYSNKIL RQIDLLAAE KGETERLNP EVGALIIID RSVDYPSAL LTPGTYSAL LNEVYGVTT GMVECKESE DEVRLNPVV KKQPVKFTL DSNLDSIYS EIKNLYFTE VTSSLRTMT RKLKSEALE SRDMGLNEM RHFVQKLLQ PAQTRKKLI ANHLLAAET IANVLGPRY ETQREIEAN IIQNTNRAS NFSHLEESL CTEDDPCVT LRLFCLLCV TQKLTDSEI KNFWRKFLH QFGFASSSA MRYLINANF VPEAGVSGL PKIRFPKFT TKEFYANAN KFKQIPANP EFADLGHPG CASYVFGGI YIPLVTKIA GMIVSGTPI EEIRSKLEG LGMVTLRSG DAYPLERRT VLIYFVGGV TYAEIAACN LLETFTRAT IVVMSDRIV SGNDLMKQM LS
- >TC001243 RA
atggatgta aaccaaaga ttatcgtct tttcaagag atttccaag accctgctt tcgaaaatc ttcagttct gtccctcgg acgaaacat atcgttatc gaaccgagt gtgattcgc ccccttgag cgcgtttgt agcgtgagc tggctgaag ggaaacggc attgagaag atatttaaa ctggaaggg aaatgcccg aattttggc aacagcccc gttttttac atgattttc aatcaaagg gctacattc cgacgagtg gttgatcag attcggtca cagatcgac caagagaat ccagttaag aataggttt cacgtaatc attgtccca cactatcag cgttgtttt gacgacatg ttagaagca ctgggcttg tgttccatt atcaagtta cactgcttc ctttggttt cctgtccac ttggacacg gggatttta agtttggaa ttgccccac atttacaag aaactgttt ttggtgcat gactacacg gccttgcac cccttggcc agagcccta tggaacatg ttcagtgtg attggttta ccaaagttc cagatagct gtaggcgaa tattcgaat aaaattttg cgccagatt gacttgctc gccgccgag aagggcgaa accgagcga cttaatccc gaggtgggg gctttaatc ataattgac aggagcgta gattaccca agtgcctta ctaacacct ggtacctat tccgcttta ctaaacgaa gtgtacgga gttactact gggatggtc gagtgcaag gaaagcgag gatgaagtc cgccttaat ccagtcgta aaaaaacag ccggtgaaa ttcaccctc gatagcaat ctcgactca atttactcc gagataaaa aacctgtat ttcacggaa gttacgtca agtttgagg accatgacg cgcaaatta aaatccgag gcgctcgag tcgcgtgac atgggctta aacgaaatg cgccacttc gtgcaaaag ctcctccag cccgcacaa actcgtaaa aaactaatc gccaatcat ttgcttgcc gccgagacc atagccaat gtcctcggc ccgcgctac gaaacgcag cgcgagatc gaggcgaac atcatccag aacacgaac cgcgcctcc aacttctcc cacctggag gagagtctg tgcacggaa gacgacccg tgtgtcaca ctgcggctc ttttgtttg ctctgtgtc acgcagaaa ctcaccgac agcgaaatt aaaaacttc tggaggaag tttctacac cagttcggg ttcgcctcc agttcggct atgcgttat ttgatcaac gctaatttc gtgccggag gcgggggtt tcgggactg ccgaaaata cgcttcccg aagttcaca acgaaggag ttttacgcc aatgcgaat aagtttaaa cagattccg gctaatccg gagtttgcc gatttgggg catccgggg tgtgcgagt tatgtcttt ggggggatt tacataccg ttggttacg aaaatcgcg ggtatgatc gttagtggc actccgatt gaggagatt aggagtaaa ttggagggg cttgggatg gtcacgttg aggagtggt gacgcctat cctttggaa aggcggact gtgctcatt tatttcgtt ggaggggtc acgtatgcc gaaattgca gcttgtaat cttttggag actttcacg agggctaca attgtcgtt atgagcgac aggattgtc tcaggaaac gatttgatg aagcaaatg ctgtcttaa
- >TC001243 RA
tgataagtt atttactaa taatggatg taaaccaaa gattatcgt cttttcaag agatttcca agaccctgc tttcgaaaa tcttcagtt ctgtccctc ggacgaaac atatcgtta tcgaaccga gtgtgattc gcccccttg agcgcgttt gtagcgtga gctggctga agggaaacg gcattgaga agatattta aactggaag ggaaatgcc cgaattttg gcaacagcc ccgtttttt acatgattt tcaatcaaa gggctacat tccgacgag tggttgatc agattcggt cacagatcg accaagaga atccagtta agaataggt ttcacgtaa tcattgtcc cacactatc agcgttgtt ttgacgaca tgttagaag cactgggct tgtgttcca ttatcaagt tacactgct tcctttggt ttcctgtcc acttggaca cggggattt taagtttgg aattgcccc acatttaca agaaactgt ttttggtgc atgactaca cggccttgc accccttgg ccagagccc tatggaaca tgttcagtg tgattggtt taccaaagt tccagatag ctgtaggcg aatattcga ataaaattt tgcgccaga ttgacttgc tcgccgccg agaagggcg aaaccgagc gacttaatc ccgaggtgg gggctttaa tcataattg acaggagcg tagattacc caagtgcct tactaacac ctggtacct attccgctt tactaaacg aagtgtacg gagttacta ctgggatgg tcgagtgca aggaaagcg aggatgaag tccgcctta atccagtcg taaaaaaac agccggtga aattcaccc tcgatagca atctcgact caatttact ccgagataa aaaacctgt atttcacgg aagttacgt caagtttga ggaccatga cgcgcaaat taaaatccg aggcgctcg agtcgcgtg acatgggct taaacgaaa tgcgccact tcgtgcaaa agctcctcc agcccgcac aaactcgta aaaaactaa tcgccaatc atttgcttg ccgccgaga ccatagcca atgtcctcg gcccgcgct acgaaacgc agcgcgaga tcgaggcga acatcatcc agaacacga accgcgcct ccaacttct cccacctgg aggagagtc tgtgcacgg aagacgacc cgtgtgtca cactgcggc tcttttgtt tgctctgtg tcacgcaga aactcaccg acagcgaaa ttaaaaact tctggagga agtttctac accagttcg ggttcgcct ccagttcgg ctatgcgtt atttgatca acgctaatt tcgtgccgg aggcggggg tttcgggac tgccgaaaa tacgcttcc cgaagttca caacgaagg agttttacg ccaatgcga ataagttta aacagattc cggctaatc cggagtttg ccgatttgg ggcatccgg ggtgtgcga gttatgtct ttgggggga tttacatac cgttggtta cgaaaatcg cgggtatga tcgttagtg gcactccga ttgaggaga ttaggagta aattggagg ggcttggga tggtcacgt tgaggagtg gtgacgcct atcctttgg aaaggcgga ctgtgctca tttatttcg ttggagggg tcacgtatg ccgaaattg cagcttgta atcttttgg agactttca cgagggcta caattgtcg ttatgagcg acaggattg tctcaggaa acgatttga tgaagcaaa tgctgtctt aaaccattt tgtattatt ttttctaca aaaacagtc aaaaaatca cgtttttgt ttcaataac ttgtacgac tgttttgtt tgccaaatt ctatatttt gtaccgttg tttttatta aaataaacg aaaaaaagt tca
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
