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Gene information
General information
TC032221
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC001235,TC001236
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 13068615..13070815
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032221
    iBeetle screen iB_07897

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    tropharium structure irregular - Number of animals showing the phenotype: 4

    vitellarium size decreased - Number of animals showing the phenotype: 4
    egg chamber mostly not present - Number of animals showing the phenotype: 4

    mature egg not present - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032221 RA
      MTDEPEKDI NDVFSDILL SEDRIFEQS YNQGYELGK 
      GEENIEAYH LGYHRGAEL GAEIGYYTS ITSYYLSHK 
      SGDEKIVKE LDTLKEMLN SFPRENDPN VDILELIGK 
      IRAKFKKIC AVLKLQPSF PEQTIDSLL AFLEPLLGF 
      ANCHMVDFY TQNSYKKFV SPEIQNEIE QIGYENTIK 
      RIFLNEFDA TPHLKQFVE DSSKFTLKN CHVCLNLDS 
      FTQKLQSWG CDTLDTFKL EIFMNAKKS HEVEILSAV 
      AAALFRVSQ ASHVVDLGD GKGYLSSML ALQHQIPVV 
      GIDASNTNT CGAIKRATK LSKVWNGIP KAPHKSLPK 
      KTENFASPH VELYKQVTR FVDERFDLL GLVRDVFPN 
      VSHLGLVGL HTCGDLAAS SLKIFSRNE AVKSVCNVG 
      CCYHLLDES GFPLSRFLT DRGFVLGRS ARMIANQSV 
      ERVLQEGEL PNITIFYRA ILQVLLEEF CTDLPTKHV 
      GKFRKVPVN FLDYVRLAL KRIDVTLDL TDNEVGAIF 
      SRYEKRLNE LNVFYLLRC KLSPVVESL ILLDRLLFL 
      QEQGFENSF LVQFFDPVV SPRCYGIVA VKNAL
    CDS sequence
    • >TC032221 RA
      atgacagat gagcccgaa aaagacatt aacgatgtt 
      ttctccgac attctcctg agcgaggac cgcattttc 
      gagcaaagt tacaaccaa ggctacgaa ttaggaaaa 
      ggggaagaa aacatcgaa gcctaccac ttgggttat 
      catcgtggg gccgaatta ggtgcagaa atcggttat 
      tacaccagt attacgtca tattattta agccataaa 
      tcaggtgac gaaaaaatc gtcaaagaa ctcgatact 
      ttgaaggaa atgttaaat tcttttccc agagaaaat 
      gacccaaat gttgatata ttagaactc ataggcaaa 
      attcgagcg aaattcaaa aaaatctgc gcggttttg 
      aaactacaa ccctctttt cccgaacaa acaattgac 
      agtcttttg gcgtttctg gaaccattg ttgggtttt 
      gccaactgt cacatggta gatttttac acccagaat 
      tcctacaaa aaatttgta tctccggaa attcaaaac 
      gaaatcgaa caaattggt tacgaaaac acaatcaag 
      cgcattttc ctgaacgag tttgacgcg acccctcat 
      ttgaaacaa tttgtcgaa gacagctca aaatttacg 
      ttaaaaaac tgccacgtg tgtctcaat ttggactct 
      ttcactcaa aaactgcaa tcgtggggc tgtgatact 
      ttagatacg ttcaaactc gagattttc atgaatgcg 
      aaaaaatcg cacgaggtt gagatttta agcgccgtg 
      gccgcggcc ctattccgg gtctcgcaa gcgtctcat 
      gtggtggat ttgggcgat ggcaagggc tatctaagc 
      tcgatgtta gccctccaa caccaaatc ccggtcgtg 
      ggaatagac gcgtccaac actaacact tgtggggct 
      atcaagcgc gccaccaaa ctctccaaa gtgtggaac 
      ggcatcccc aaggcgccc cacaaatcg ctccctaaa 
      aaaaccgaa aatttcgcc agcccccac gttgaactt 
      tacaaacaa gtcacgcgg tttgttgac gaaaggttc 
      gacttgttg gggcttgtg cgggacgtt tttcccaac 
      gtgtctcac ctggggctc gtggggctc cacacgtgt 
      ggcgacttg gctgccagt tccttgaaa atttttagt 
      agaaatgag gcagtcaag tcggtctgt aacgtgggg 
      tgttgttat catttgctg gacgaatcg gggtttccc 
      ctaagtagg tttctcaca gatcggggg tttgttctt 
      ggacgaagc gcccgaatg attgcgaat caatcggtc 
      gagagggtt ttacaagag ggcgagttg ccaaacatt 
      acgattttt tatcgcgcg attttgcaa gtcttgctt 
      gaggagttt tgtacagat ttgcccacg aaacatgtc 
      gggaagttt cggaaagtg ccggttaat tttttggac 
      tacgtccga ctagcttta aaacgaatt gacgtcact 
      cttgacttg actgataac gaagttggg gctattttt 
      tcacgttat gagaaacga ctcaatgaa ttgaatgtt 
      ttttacttg ttacggtgc aagttatca ccggttgtt 
      gaaagtttg attttgctt gatcgcctc ttgttttta 
      caagagcaa ggttttgag aattcgttt ctggtccag 
      tttttcgat cctgttgtg tcaccccga tgttatggc 
      attgttgca gttaaaaat gcattgtaa
    mRNA sequence
    • >TC032221 RA
      agggtttaa aatcctaac cttttttta tggtgcaat 
      tttttgatt gaaactttg tgattattg taccgattt 
      tttgtaaat ctgcaatta tcatttgtg ttacacacg 
      tttcgcaag ttttgtgct tgataagcg ttatgcaag 
      accatgaca gatgagccc gaaaaagac attaacgat 
      gttttctcc gacattctc ctgagcgag gaccgcatt 
      ttcgagcaa agttacaac caaggctac gaattagga 
      aaaggggaa gaaaacatc gaagcctac cacttgggt 
      tatcatcgt ggggccgaa ttaggtgca gaaatcggt 
      tattacacc agtattacg tcatattat ttaagccat 
      aaatcaggt gacgaaaaa atcgtcaaa gaactcgat 
      actttgaag gaaatgtta aattctttt cccagagaa 
      aatgaccca aatgttgat atattagaa ctcataggc 
      aaaattcga gcgaaattc aaaaaaatc tgcgcggtt 
      ttgaaacta caaccctct tttcccgaa caaacaatt 
      gacagtctt ttggcgttt ctggaacca ttgttgggt 
      tttgccaac tgtcacatg gtagatttt tacacccag 
      aattcctac aaaaaattt gtatctccg gaaattcaa 
      aacgaaatc gaacaaatt ggttacgaa aacacaatc 
      aagcgcatt ttcctgaac gagtttgac gcgacccct 
      catttgaaa caatttgtc gaagacagc tcaaaattt 
      acgttaaaa aactgccac gtgtgtctc aatttggac 
      tctttcact caaaaactg caatcgtgg ggctgtgat 
      actttagat acgttcaaa ctcgagatt ttcatgaat 
      gcgaaaaaa tcgcacgag gttgagatt ttaagcgcc 
      gtggccgcg gccctattc cgggtctcg caagcgtct 
      catgtggtg gatttgggc gatggcaag ggctatcta 
      agctcgatg ttagccctc caacaccaa atcccggtc 
      gtgggaata gacgcgtcc aacactaac acttgtggg 
      gctatcaag cgcgccacc aaactctcc aaagtgtgg 
      aacggcatc cccaaggcg ccccacaaa tcgctccct 
      aaaaaaacc gaaaatttc gccagcccc cacgttgaa 
      ctttacaaa caagtcacg cggtttgtt gacgaaagg 
      ttcgacttg ttggggctt gtgcgggac gtttttccc 
      aacgtgtct cacctgggg ctcgtgggg ctccacacg 
      tgtggcgac ttggctgcc agttccttg aaaattttt 
      agtagaaat gaggcagtc aagtcggtc tgtaacgtg 
      gggtgttgt tatcatttg ctggacgaa tcggggttt 
      cccctaagt aggtttctc acagatcgg gggtttgtt 
      cttggacga agcgcccga atgattgcg aatcaatcg 
      gtcgagagg gttttacaa gagggcgag ttgccaaac 
      attacgatt ttttatcgc gcgattttg caagtcttg 
      cttgaggag ttttgtaca gatttgccc acgaaacat 
      gtcgggaag tttcggaaa gtgccggtt aattttttg 
      gactacgtc cgactagct ttaaaacga attgacgtc 
      actcttgac ttgactgat aacgaagtt ggggctatt 
      ttttcacgt tatgagaaa cgactcaat gaattgaat 
      gttttttac ttgttacgg tgcaagtta tcaccggtt 
      gttgaaagt ttgattttg cttgatcgc ctcttgttt 
      ttacaagag caaggtttt gagaattcg tttctggtc 
      cagtttttc gatcctgtt gtgtcaccc cgatgttat 
      ggcattgtt gcagttaaa aatgcattg taatggatt 
      gaataaaac acaatactg gtttcattc ttttattaa 
      tttgtttac aactaaacc catttagaa aaaaggtta 
      cacgattta agtacctac actgcaata tttcagtcg 
      tttctaaat taaagcgcg gctaattta tgactaagg 
      tttgcatat aaaaaatac atttcattt gtagatggc 
      aagccagta aaaaacaat tacgttaca tattttaca 
      ataaaaatt tccgtaaaa c
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY