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Gene information
General information
TC001202 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 12579938..12584395
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC001202
    iBeetle screen iB_07894

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection

    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 3
    mature egg not present - Number of animals showing the phenotype: 1
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001202 RA
      MKKVKNSTD IHQKQGLEQ SKKLYRSTT EQAKRLDDA 
RSCARTISD LFTPNLQIQ RRKFCEFCE RLIFSDPVL 
YGKKGEELL WRKGYYDVV STAKKLKKK EYTQNEICN 
LLAHVNSGI GFYHHMISK LQCEFNLNL THVIDFSMG 
GAFGKGDAR TECLEWANL SVHQCLIYL GDLSRYKLE 
IDPKIGATL AFRYYLQAI SFKPDHGMP HNQMGTLAM 
NQNKFLDAV YHYMRCLAC PVSFDGTTN NLHSLFDKN 
SKYLEQLPV EPNSDSNTQ DDHLKRFIS RFLLLIDIW 
YFNKKVAKV YSLCHQTYK DLEECLQLK TSNESCESP 
TGSETVETD SAEQLNNDM IFKMIVICL LCISKLQKT 
NQHQVSTAV AFTLAIYSQ LVQNVANHI QESVLNYPL 
PEVEVKIEQ NGVVKDLLK KSKKKRNAS QLRRRKALP 
VEIESDDSE REDDEVDSD SDSSDSFVS DSENILAES 
SDDETEVKK SDDSVNDDK IDVIKKIKR MDVNDMLEI 
ITEQGSLQA IKIINDWLM QDTEVIKSC GTSTRSLLR 
QMTNMLNLI NINIKSSKL SGIKLDVNN VIKNEEKIP 
LSEDIVLKG IDILEKAHK NMDWNYLHK HGMNTKEES 
VTRIVKFMA FGKFLTGIE ETGITFDER KNVFVCKCD 
ENDENKATN SVVEELEMQ ETGHVIGDK EGDKGQLMK 
MKHMGQLWL ASEVRALEN RVKGKASLS PYLVLDADA 
LIKYTFMVK HFVQSRKFI VLIPSAVVQ ALDDLKREQ 
SEARDAIRW LESQFHRGN RFFRAQRPQ ERAAIPFIK 
YPKKRDKEM STYIQIIEC CYYLAEQQK GAANLITLL 
IGNQNVLSN GENKEFSYV GLAQSAGIS IESITQFYS 
KWKKSYKTR R
    CDS sequence
    • >TC001202 RA
      atgaagaag gttaaaaat tctaccgat atccaccaa 
aagcagggc cttgagcag tctaaaaag ttgtacaga 
tctacaacc gagcaggca aaacgttta gatgacgct 
cgcagctgc gcccggacc atcagcgac ctattcacc 
cccaacttg caaatccaa cggcggaaa ttctgcgag 
ttctgcgaa cggttgatt ttttccgac cccgtcctg 
tatggaaaa aaaggagag gagttactg tggcgcaaa 
ggctattat gacgtcgtt tccaccgcg aaaaaactc 
aaaaagaag gaatacacc cagaacgaa atttgcaat 
ttactggca catgttaat tccggcatt ggcttctac 
caccacatg atatccaaa ctgcagtgc gaatttaat 
ttaaatctg actcatgtc atcgatttt tcaatgggg 
ggcgccttc ggcaagggc gacgccaga acggagtgt 
ctcgagtgg gcgaacctt tcagtacac caatgtttg 
atttattta ggcgatttg agtcgatac aaactcgag 
attgacccc aaaattggg gcgactcta gctttccgc 
tattaccta caagccatt tcgttcaaa cccgaccac 
ggcatgccc cacaatcaa atggggact ttagcaatg 
aaccagaac aaatttcta gacgcggtt taccactac 
atgcgttgc ctggcgtgt cctgtttct ttcgacggg 
acaacgaat aatttgcac agtcttttc gataaaaac 
tcgaaatat ttagagcaa ctcccggtg gagccaaac 
tctgattcg aacacgcaa gacgatcat ctcaaacga 
tttatttcg cggttcctc ctccttatc gatatctgg 
tactttaat aaaaaagtt gccaaagtt tacagtttg 
tgccaccaa acgtacaaa gatttagaa gagtgtttg 
caactgaag acaagcaac gaaagttgc gagtcgccg 
accgggagc gaaacggtt gaaaccgac tcagccgaa 
cagttgaac aatgacatg atttttaaa atgatcgtc 
atttgtctc ctatgtatc tccaaactg cagaaaaca 
aaccagcat caagtctca actgctgtt gccttcacc 
cttgccatt tattcccag ttggttcaa aacgtggcc 
aatcacatc caagaaagt gtccttaat tatcccttg 
ccggaggtg gaggttaaa attgagcag aacggggtt 
gttaaagat ctactgaag aagagtaaa aagaagcga 
aacgcgtct cagttgcgt cgcaggaaa gcacttcct 
gtcgaaatc gagtcggat gatagcgaa cgtgaagac 
gacgaagtc gattcggat tcggattcc tccgattcg 
tttgtctct gactcggag aatattttg gccgaatcg 
tccgacgat gagactgaa gttaaaaaa tcggacgat 
tcggtcaat gacgataaa atcgacgtg attaagaaa 
atcaaacgg atggacgtg aacgacatg ctcgagata 
ataacagag caaggctcc ctccaagcg atcaaaatc 
ataaacgac tggttgatg caagacacc gaagttata 
aaatcctgc ggtacaagt actcgcagt cttttacgt 
caaatgaca aacatgctg aacctgatt aatatcaat 
ataaaaagc agcaaacta tccggtatt aaattggac 
gtgaataat gttataaaa aacgaagag aaaatcccg 
ttgtcggaa gatattgtt ttgaaaggt attgacata 
ttggagaaa gctcacaag aatatggat tggaattat 
ttacataaa catggaatg aacacgaaa gaagagagc 
gtcacaagg atcgtaaaa tttatggcg tttggaaag 
tttctgacc ggaattgag gagaccggg atcactttt 
gacgaacgg aaaaatgtt tttgtttgt aaatgcgac 
gaaaacgac gagaataaa gcgaccaat tcggtcgtc 
gaggagttg gaaatgcaa gaaaccggc cacgtaatc 
ggtgataag gagggtgat aagggccaa cttatgaaa 
atgaaacat atggggcag ttgtggctg gcgtctgaa 
gtacgagcg ttggaaaat agagtgaaa ggaaaggct 
tcgctctcg ccctatttg gttttagac gctgacgcc 
ttaattaaa tatacattt atggtcaaa catttcgtg 
cagtcgagg aaatttatc gtcttaata ccatctgca 
gttgtacag gctttggac gatttaaaa cgggagcag 
agtgaagct cgtgacgct atccgttgg ttggagagt 
caatttcat cgcggaaac cgatttttt cgggctcaa 
cgaccccaa gaacgcgcg gcgattcct ttcatcaag 
tatccgaag aagcgagat aaggaaatg tcgacgtac 
atccagatc attgagtgt tgctactac ttggccgaa 
caacagaaa ggagccgct aatctaatt actttactt 
attgggaat cagaacgtt ttgtcgaac ggcgaaaat 
aaggaattt agttacgtg ggcttggcg cagtcggca 
gggatttcg atcgaatca attacgcag ttttatagt 
aaatggaag aagagttac aagacgagg agatga
    mRNA sequence
    • >TC001202 RA
      ccgcattaa aatatagtt tgggggcga attttggtt 
aaattgagt cgatggttt ggctgtttt acgcgacca 
aattttccg tgccgctag atggcggcg taaaatttc 
tcaaaatca tgtggcaat ggacatatt tgacgattt 
gataagaaa ttgtgatgg tgtcaaaac atatttgtg 
aaattaacc atgatagaa aaatgagtt gtgtggccg 
ttcggtatt tggaatcac taatcgagt tcaaaggaa 
gtttgttca attcttgac cggggtagc aacatgagt 
tgaaatgaa gaaggttaa aaattctac cgatatcca 
ccaaaagca gggccttga gcagtctaa aaagttgta 
cagatctac aaccgagca ggcaaaacg tttagatga 
cgctcgcag ctgcgcccg gaccatcag cgacctatt 
cacccccaa cttgcaaat ccaacggcg gaaattctg 
cgagttctg cgaacggtt gattttttc cgaccccgt 
cctgtatgg aaaaaaagg agaggagtt actgtggcg 
caaaggcta ttatgacgt cgtttccac cgcgaaaaa 
actcaaaaa gaaggaata cacccagaa cgaaatttg 
caatttact ggcacatgt taattccgg cattggctt 
ctaccacca catgatatc caaactgca gtgcgaatt 
taatttaaa tctgactca tgtcatcga tttttcaat 
ggggggcgc cttcggcaa gggcgacgc cagaacgga 
gtgtctcga gtgggcgaa cctttcagt acaccaatg 
tttgattta tttaggcga tttgagtcg atacaaact 
cgagattga ccccaaaat tggggcgac tctagcttt 
ccgctatta cctacaagc catttcgtt caaacccga 
ccacggcat gccccacaa tcaaatggg gactttagc 
aatgaacca gaacaaatt tctagacgc ggtttacca 
ctacatgcg ttgcctggc gtgtcctgt ttctttcga 
cgggacaac gaataattt gcacagtct tttcgataa 
aaactcgaa atatttaga gcaactccc ggtggagcc 
aaactctga ttcgaacac gcaagacga tcatctcaa 
acgatttat ttcgcggtt cctcctcct tatcgatat 
ctggtactt taataaaaa agttgccaa agtttacag 
tttgtgcca ccaaacgta caaagattt agaagagtg 
tttgcaact gaagacaag caacgaaag ttgcgagtc 
gccgaccgg gagcgaaac ggttgaaac cgactcagc 
cgaacagtt gaacaatga catgatttt taaaatgat 
cgtcatttg tctcctatg tatctccaa actgcagaa 
aacaaacca gcatcaagt ctcaactgc tgttgcctt 
cacccttgc catttattc ccagttggt tcaaaacgt 
ggccaatca catccaaga aagtgtcct taattatcc 
cttgccgga ggtggaggt taaaattga gcagaacgg 
ggttgttaa agatctact gaagaagag taaaaagaa 
gcgaaacgc gtctcagtt gcgtcgcag gaaagcact 
tcctgtcga aatcgagtc ggatgatag cgaacgtga 
agacgacga agtcgattc ggattcgga ttcctccga 
ttcgtttgt ctctgactc ggagaatat tttggccga 
atcgtccga cgatgagac tgaagttaa aaaatcgga 
cgattcggt caatgacga taaaatcga cgtgattaa 
gaaaatcaa acggatgga cgtgaacga catgctcga 
gataataac agagcaagg ctccctcca agcgatcaa 
aatcataaa cgactggtt gatgcaaga caccgaagt 
tataaaatc ctgcggtac aagtactcg cagtctttt 
acgtcaaat gacaaacat gctgaacct gattaatat 
caatataaa aagcagcaa actatccgg tattaaatt 
ggacgtgaa taatgttat aaaaaacga agagaaaat 
cccgttgtc ggaagatat tgttttgaa aggtattga 
catattgga gaaagctca caagaatat ggattggaa 
ttatttaca taaacatgg aatgaacac gaaagaaga 
gagcgtcac aaggatcgt aaaatttat ggcgtttgg 
aaagtttct gaccggaat tgaggagac cgggatcac 
ttttgacga acggaaaaa tgtttttgt ttgtaaatg 
cgacgaaaa cgacgagaa taaagcgac caattcggt 
cgtcgagga gttggaaat gcaagaaac cggccacgt 
aatcggtga taaggaggg tgataaggg ccaacttat 
gaaaatgaa acatatggg gcagttgtg gctggcgtc 
tgaagtacg agcgttgga aaatagagt gaaaggaaa 
ggcttcgct ctcgcccta tttggtttt agacgctga 
cgccttaat taaatatac atttatggt caaacattt 
cgtgcagtc gaggaaatt tatcgtctt aataccatc 
tgcagttgt acaggcttt ggacgattt aaaacggga 
gcagagtga agctcgtga cgctatccg ttggttgga 
gagtcaatt tcatcgcgg aaaccgatt ttttcgggc 
tcaacgacc ccaagaacg cgcggcgat tcctttcat 
caagtatcc gaagaagcg agataagga aatgtcgac 
gtacatcca gatcattga gtgttgcta ctacttggc 
cgaacaaca gaaaggagc cgctaatct aattacttt 
acttattgg gaatcagaa cgttttgtc gaacggcga 
aaataagga atttagtta cgtgggctt ggcgcagtc 
ggcagggat ttcgatcga atcaattac gcagtttta 
tagtaaatg gaagaagag ttacaagac gaggagatg 
aatggcgaa ttgagcggg atatgggat tatgagctc 
atcggatcg tgcgggtgc aggcggtgg ttgtgaaga 
attgttcat tttggaatt ttaaccgcg aactttatt 
catttcaac aggatttgt agttttact tgttttgtc 
agcaataaa ggcttattt tactg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY