| General information | |||
| TC001202 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 12579938..12584395 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC001202 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Reference | * {{ blurReferenceResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_07894 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
mature egg not present - Number of animals showing the phenotype: 1
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC001202 RA
MKKVKNSTD IHQKQGLEQ SKKLYRSTT EQAKRLDDA RSCARTISD LFTPNLQIQ RRKFCEFCE RLIFSDPVL YGKKGEELL WRKGYYDVV STAKKLKKK EYTQNEICN LLAHVNSGI GFYHHMISK LQCEFNLNL THVIDFSMG GAFGKGDAR TECLEWANL SVHQCLIYL GDLSRYKLE IDPKIGATL AFRYYLQAI SFKPDHGMP HNQMGTLAM NQNKFLDAV YHYMRCLAC PVSFDGTTN NLHSLFDKN SKYLEQLPV EPNSDSNTQ DDHLKRFIS RFLLLIDIW YFNKKVAKV YSLCHQTYK DLEECLQLK TSNESCESP TGSETVETD SAEQLNNDM IFKMIVICL LCISKLQKT NQHQVSTAV AFTLAIYSQ LVQNVANHI QESVLNYPL PEVEVKIEQ NGVVKDLLK KSKKKRNAS QLRRRKALP VEIESDDSE REDDEVDSD SDSSDSFVS DSENILAES SDDETEVKK SDDSVNDDK IDVIKKIKR MDVNDMLEI ITEQGSLQA IKIINDWLM QDTEVIKSC GTSTRSLLR QMTNMLNLI NINIKSSKL SGIKLDVNN VIKNEEKIP LSEDIVLKG IDILEKAHK NMDWNYLHK HGMNTKEES VTRIVKFMA FGKFLTGIE ETGITFDER KNVFVCKCD ENDENKATN SVVEELEMQ ETGHVIGDK EGDKGQLMK MKHMGQLWL ASEVRALEN RVKGKASLS PYLVLDADA LIKYTFMVK HFVQSRKFI VLIPSAVVQ ALDDLKREQ SEARDAIRW LESQFHRGN RFFRAQRPQ ERAAIPFIK YPKKRDKEM STYIQIIEC CYYLAEQQK GAANLITLL IGNQNVLSN GENKEFSYV GLAQSAGIS IESITQFYS KWKKSYKTR R
- >TC001202 RA
atgaagaag gttaaaaat tctaccgat atccaccaa aagcagggc cttgagcag tctaaaaag ttgtacaga tctacaacc gagcaggca aaacgttta gatgacgct cgcagctgc gcccggacc atcagcgac ctattcacc cccaacttg caaatccaa cggcggaaa ttctgcgag ttctgcgaa cggttgatt ttttccgac cccgtcctg tatggaaaa aaaggagag gagttactg tggcgcaaa ggctattat gacgtcgtt tccaccgcg aaaaaactc aaaaagaag gaatacacc cagaacgaa atttgcaat ttactggca catgttaat tccggcatt ggcttctac caccacatg atatccaaa ctgcagtgc gaatttaat ttaaatctg actcatgtc atcgatttt tcaatgggg ggcgccttc ggcaagggc gacgccaga acggagtgt ctcgagtgg gcgaacctt tcagtacac caatgtttg atttattta ggcgatttg agtcgatac aaactcgag attgacccc aaaattggg gcgactcta gctttccgc tattaccta caagccatt tcgttcaaa cccgaccac ggcatgccc cacaatcaa atggggact ttagcaatg aaccagaac aaatttcta gacgcggtt taccactac atgcgttgc ctggcgtgt cctgtttct ttcgacggg acaacgaat aatttgcac agtcttttc gataaaaac tcgaaatat ttagagcaa ctcccggtg gagccaaac tctgattcg aacacgcaa gacgatcat ctcaaacga tttatttcg cggttcctc ctccttatc gatatctgg tactttaat aaaaaagtt gccaaagtt tacagtttg tgccaccaa acgtacaaa gatttagaa gagtgtttg caactgaag acaagcaac gaaagttgc gagtcgccg accgggagc gaaacggtt gaaaccgac tcagccgaa cagttgaac aatgacatg atttttaaa atgatcgtc atttgtctc ctatgtatc tccaaactg cagaaaaca aaccagcat caagtctca actgctgtt gccttcacc cttgccatt tattcccag ttggttcaa aacgtggcc aatcacatc caagaaagt gtccttaat tatcccttg ccggaggtg gaggttaaa attgagcag aacggggtt gttaaagat ctactgaag aagagtaaa aagaagcga aacgcgtct cagttgcgt cgcaggaaa gcacttcct gtcgaaatc gagtcggat gatagcgaa cgtgaagac gacgaagtc gattcggat tcggattcc tccgattcg tttgtctct gactcggag aatattttg gccgaatcg tccgacgat gagactgaa gttaaaaaa tcggacgat tcggtcaat gacgataaa atcgacgtg attaagaaa atcaaacgg atggacgtg aacgacatg ctcgagata ataacagag caaggctcc ctccaagcg atcaaaatc ataaacgac tggttgatg caagacacc gaagttata aaatcctgc ggtacaagt actcgcagt cttttacgt caaatgaca aacatgctg aacctgatt aatatcaat ataaaaagc agcaaacta tccggtatt aaattggac gtgaataat gttataaaa aacgaagag aaaatcccg ttgtcggaa gatattgtt ttgaaaggt attgacata ttggagaaa gctcacaag aatatggat tggaattat ttacataaa catggaatg aacacgaaa gaagagagc gtcacaagg atcgtaaaa tttatggcg tttggaaag tttctgacc ggaattgag gagaccggg atcactttt gacgaacgg aaaaatgtt tttgtttgt aaatgcgac gaaaacgac gagaataaa gcgaccaat tcggtcgtc gaggagttg gaaatgcaa gaaaccggc cacgtaatc ggtgataag gagggtgat aagggccaa cttatgaaa atgaaacat atggggcag ttgtggctg gcgtctgaa gtacgagcg ttggaaaat agagtgaaa ggaaaggct tcgctctcg ccctatttg gttttagac gctgacgcc ttaattaaa tatacattt atggtcaaa catttcgtg cagtcgagg aaatttatc gtcttaata ccatctgca gttgtacag gctttggac gatttaaaa cgggagcag agtgaagct cgtgacgct atccgttgg ttggagagt caatttcat cgcggaaac cgatttttt cgggctcaa cgaccccaa gaacgcgcg gcgattcct ttcatcaag tatccgaag aagcgagat aaggaaatg tcgacgtac atccagatc attgagtgt tgctactac ttggccgaa caacagaaa ggagccgct aatctaatt actttactt attgggaat cagaacgtt ttgtcgaac ggcgaaaat aaggaattt agttacgtg ggcttggcg cagtcggca gggatttcg atcgaatca attacgcag ttttatagt aaatggaag aagagttac aagacgagg agatga
- >TC001202 RA
ccgcattaa aatatagtt tgggggcga attttggtt aaattgagt cgatggttt ggctgtttt acgcgacca aattttccg tgccgctag atggcggcg taaaatttc tcaaaatca tgtggcaat ggacatatt tgacgattt gataagaaa ttgtgatgg tgtcaaaac atatttgtg aaattaacc atgatagaa aaatgagtt gtgtggccg ttcggtatt tggaatcac taatcgagt tcaaaggaa gtttgttca attcttgac cggggtagc aacatgagt tgaaatgaa gaaggttaa aaattctac cgatatcca ccaaaagca gggccttga gcagtctaa aaagttgta cagatctac aaccgagca ggcaaaacg tttagatga cgctcgcag ctgcgcccg gaccatcag cgacctatt cacccccaa cttgcaaat ccaacggcg gaaattctg cgagttctg cgaacggtt gattttttc cgaccccgt cctgtatgg aaaaaaagg agaggagtt actgtggcg caaaggcta ttatgacgt cgtttccac cgcgaaaaa actcaaaaa gaaggaata cacccagaa cgaaatttg caatttact ggcacatgt taattccgg cattggctt ctaccacca catgatatc caaactgca gtgcgaatt taatttaaa tctgactca tgtcatcga tttttcaat ggggggcgc cttcggcaa gggcgacgc cagaacgga gtgtctcga gtgggcgaa cctttcagt acaccaatg tttgattta tttaggcga tttgagtcg atacaaact cgagattga ccccaaaat tggggcgac tctagcttt ccgctatta cctacaagc catttcgtt caaacccga ccacggcat gccccacaa tcaaatggg gactttagc aatgaacca gaacaaatt tctagacgc ggtttacca ctacatgcg ttgcctggc gtgtcctgt ttctttcga cgggacaac gaataattt gcacagtct tttcgataa aaactcgaa atatttaga gcaactccc ggtggagcc aaactctga ttcgaacac gcaagacga tcatctcaa acgatttat ttcgcggtt cctcctcct tatcgatat ctggtactt taataaaaa agttgccaa agtttacag tttgtgcca ccaaacgta caaagattt agaagagtg tttgcaact gaagacaag caacgaaag ttgcgagtc gccgaccgg gagcgaaac ggttgaaac cgactcagc cgaacagtt gaacaatga catgatttt taaaatgat cgtcatttg tctcctatg tatctccaa actgcagaa aacaaacca gcatcaagt ctcaactgc tgttgcctt cacccttgc catttattc ccagttggt tcaaaacgt ggccaatca catccaaga aagtgtcct taattatcc cttgccgga ggtggaggt taaaattga gcagaacgg ggttgttaa agatctact gaagaagag taaaaagaa gcgaaacgc gtctcagtt gcgtcgcag gaaagcact tcctgtcga aatcgagtc ggatgatag cgaacgtga agacgacga agtcgattc ggattcgga ttcctccga ttcgtttgt ctctgactc ggagaatat tttggccga atcgtccga cgatgagac tgaagttaa aaaatcgga cgattcggt caatgacga taaaatcga cgtgattaa gaaaatcaa acggatgga cgtgaacga catgctcga gataataac agagcaagg ctccctcca agcgatcaa aatcataaa cgactggtt gatgcaaga caccgaagt tataaaatc ctgcggtac aagtactcg cagtctttt acgtcaaat gacaaacat gctgaacct gattaatat caatataaa aagcagcaa actatccgg tattaaatt ggacgtgaa taatgttat aaaaaacga agagaaaat cccgttgtc ggaagatat tgttttgaa aggtattga catattgga gaaagctca caagaatat ggattggaa ttatttaca taaacatgg aatgaacac gaaagaaga gagcgtcac aaggatcgt aaaatttat ggcgtttgg aaagtttct gaccggaat tgaggagac cgggatcac ttttgacga acggaaaaa tgtttttgt ttgtaaatg cgacgaaaa cgacgagaa taaagcgac caattcggt cgtcgagga gttggaaat gcaagaaac cggccacgt aatcggtga taaggaggg tgataaggg ccaacttat gaaaatgaa acatatggg gcagttgtg gctggcgtc tgaagtacg agcgttgga aaatagagt gaaaggaaa ggcttcgct ctcgcccta tttggtttt agacgctga cgccttaat taaatatac atttatggt caaacattt cgtgcagtc gaggaaatt tatcgtctt aataccatc tgcagttgt acaggcttt ggacgattt aaaacggga gcagagtga agctcgtga cgctatccg ttggttgga gagtcaatt tcatcgcgg aaaccgatt ttttcgggc tcaacgacc ccaagaacg cgcggcgat tcctttcat caagtatcc gaagaagcg agataagga aatgtcgac gtacatcca gatcattga gtgttgcta ctacttggc cgaacaaca gaaaggagc cgctaatct aattacttt acttattgg gaatcagaa cgttttgtc gaacggcga aaataagga atttagtta cgtgggctt ggcgcagtc ggcagggat ttcgatcga atcaattac gcagtttta tagtaaatg gaagaagag ttacaagac gaggagatg aatggcgaa ttgagcggg atatgggat tatgagctc atcggatcg tgcgggtgc aggcggtgg ttgtgaaga attgttcat tttggaatt ttaaccgcg aactttatt catttcaac aggatttgt agttttact tgttttgtc agcaataaa ggcttattt tactg
