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Gene information
General information
TC001202 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 12579938..12584395
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001202
    iBeetle screen iB_07894

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 3
    mature egg not present - Number of animals showing the phenotype: 1
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001202 RA
      MKKVKNSTD IHQKQGLEQ SKKLYRSTT EQAKRLDDA 
      RSCARTISD LFTPNLQIQ RRKFCEFCE RLIFSDPVL 
      YGKKGEELL WRKGYYDVV STAKKLKKK EYTQNEICN 
      LLAHVNSGI GFYHHMISK LQCEFNLNL THVIDFSMG 
      GAFGKGDAR TECLEWANL SVHQCLIYL GDLSRYKLE 
      IDPKIGATL AFRYYLQAI SFKPDHGMP HNQMGTLAM 
      NQNKFLDAV YHYMRCLAC PVSFDGTTN NLHSLFDKN 
      SKYLEQLPV EPNSDSNTQ DDHLKRFIS RFLLLIDIW 
      YFNKKVAKV YSLCHQTYK DLEECLQLK TSNESCESP 
      TGSETVETD SAEQLNNDM IFKMIVICL LCISKLQKT 
      NQHQVSTAV AFTLAIYSQ LVQNVANHI QESVLNYPL 
      PEVEVKIEQ NGVVKDLLK KSKKKRNAS QLRRRKALP 
      VEIESDDSE REDDEVDSD SDSSDSFVS DSENILAES 
      SDDETEVKK SDDSVNDDK IDVIKKIKR MDVNDMLEI 
      ITEQGSLQA IKIINDWLM QDTEVIKSC GTSTRSLLR 
      QMTNMLNLI NINIKSSKL SGIKLDVNN VIKNEEKIP 
      LSEDIVLKG IDILEKAHK NMDWNYLHK HGMNTKEES 
      VTRIVKFMA FGKFLTGIE ETGITFDER KNVFVCKCD 
      ENDENKATN SVVEELEMQ ETGHVIGDK EGDKGQLMK 
      MKHMGQLWL ASEVRALEN RVKGKASLS PYLVLDADA 
      LIKYTFMVK HFVQSRKFI VLIPSAVVQ ALDDLKREQ 
      SEARDAIRW LESQFHRGN RFFRAQRPQ ERAAIPFIK 
      YPKKRDKEM STYIQIIEC CYYLAEQQK GAANLITLL 
      IGNQNVLSN GENKEFSYV GLAQSAGIS IESITQFYS 
      KWKKSYKTR R
    CDS sequence
    • >TC001202 RA
      atgaagaag gttaaaaat tctaccgat atccaccaa 
      aagcagggc cttgagcag tctaaaaag ttgtacaga 
      tctacaacc gagcaggca aaacgttta gatgacgct 
      cgcagctgc gcccggacc atcagcgac ctattcacc 
      cccaacttg caaatccaa cggcggaaa ttctgcgag 
      ttctgcgaa cggttgatt ttttccgac cccgtcctg 
      tatggaaaa aaaggagag gagttactg tggcgcaaa 
      ggctattat gacgtcgtt tccaccgcg aaaaaactc 
      aaaaagaag gaatacacc cagaacgaa atttgcaat 
      ttactggca catgttaat tccggcatt ggcttctac 
      caccacatg atatccaaa ctgcagtgc gaatttaat 
      ttaaatctg actcatgtc atcgatttt tcaatgggg 
      ggcgccttc ggcaagggc gacgccaga acggagtgt 
      ctcgagtgg gcgaacctt tcagtacac caatgtttg 
      atttattta ggcgatttg agtcgatac aaactcgag 
      attgacccc aaaattggg gcgactcta gctttccgc 
      tattaccta caagccatt tcgttcaaa cccgaccac 
      ggcatgccc cacaatcaa atggggact ttagcaatg 
      aaccagaac aaatttcta gacgcggtt taccactac 
      atgcgttgc ctggcgtgt cctgtttct ttcgacggg 
      acaacgaat aatttgcac agtcttttc gataaaaac 
      tcgaaatat ttagagcaa ctcccggtg gagccaaac 
      tctgattcg aacacgcaa gacgatcat ctcaaacga 
      tttatttcg cggttcctc ctccttatc gatatctgg 
      tactttaat aaaaaagtt gccaaagtt tacagtttg 
      tgccaccaa acgtacaaa gatttagaa gagtgtttg 
      caactgaag acaagcaac gaaagttgc gagtcgccg 
      accgggagc gaaacggtt gaaaccgac tcagccgaa 
      cagttgaac aatgacatg atttttaaa atgatcgtc 
      atttgtctc ctatgtatc tccaaactg cagaaaaca 
      aaccagcat caagtctca actgctgtt gccttcacc 
      cttgccatt tattcccag ttggttcaa aacgtggcc 
      aatcacatc caagaaagt gtccttaat tatcccttg 
      ccggaggtg gaggttaaa attgagcag aacggggtt 
      gttaaagat ctactgaag aagagtaaa aagaagcga 
      aacgcgtct cagttgcgt cgcaggaaa gcacttcct 
      gtcgaaatc gagtcggat gatagcgaa cgtgaagac 
      gacgaagtc gattcggat tcggattcc tccgattcg 
      tttgtctct gactcggag aatattttg gccgaatcg 
      tccgacgat gagactgaa gttaaaaaa tcggacgat 
      tcggtcaat gacgataaa atcgacgtg attaagaaa 
      atcaaacgg atggacgtg aacgacatg ctcgagata 
      ataacagag caaggctcc ctccaagcg atcaaaatc 
      ataaacgac tggttgatg caagacacc gaagttata 
      aaatcctgc ggtacaagt actcgcagt cttttacgt 
      caaatgaca aacatgctg aacctgatt aatatcaat 
      ataaaaagc agcaaacta tccggtatt aaattggac 
      gtgaataat gttataaaa aacgaagag aaaatcccg 
      ttgtcggaa gatattgtt ttgaaaggt attgacata 
      ttggagaaa gctcacaag aatatggat tggaattat 
      ttacataaa catggaatg aacacgaaa gaagagagc 
      gtcacaagg atcgtaaaa tttatggcg tttggaaag 
      tttctgacc ggaattgag gagaccggg atcactttt 
      gacgaacgg aaaaatgtt tttgtttgt aaatgcgac 
      gaaaacgac gagaataaa gcgaccaat tcggtcgtc 
      gaggagttg gaaatgcaa gaaaccggc cacgtaatc 
      ggtgataag gagggtgat aagggccaa cttatgaaa 
      atgaaacat atggggcag ttgtggctg gcgtctgaa 
      gtacgagcg ttggaaaat agagtgaaa ggaaaggct 
      tcgctctcg ccctatttg gttttagac gctgacgcc 
      ttaattaaa tatacattt atggtcaaa catttcgtg 
      cagtcgagg aaatttatc gtcttaata ccatctgca 
      gttgtacag gctttggac gatttaaaa cgggagcag 
      agtgaagct cgtgacgct atccgttgg ttggagagt 
      caatttcat cgcggaaac cgatttttt cgggctcaa 
      cgaccccaa gaacgcgcg gcgattcct ttcatcaag 
      tatccgaag aagcgagat aaggaaatg tcgacgtac 
      atccagatc attgagtgt tgctactac ttggccgaa 
      caacagaaa ggagccgct aatctaatt actttactt 
      attgggaat cagaacgtt ttgtcgaac ggcgaaaat 
      aaggaattt agttacgtg ggcttggcg cagtcggca 
      gggatttcg atcgaatca attacgcag ttttatagt 
      aaatggaag aagagttac aagacgagg agatga
    mRNA sequence
    • >TC001202 RA
      ccgcattaa aatatagtt tgggggcga attttggtt 
      aaattgagt cgatggttt ggctgtttt acgcgacca 
      aattttccg tgccgctag atggcggcg taaaatttc 
      tcaaaatca tgtggcaat ggacatatt tgacgattt 
      gataagaaa ttgtgatgg tgtcaaaac atatttgtg 
      aaattaacc atgatagaa aaatgagtt gtgtggccg 
      ttcggtatt tggaatcac taatcgagt tcaaaggaa 
      gtttgttca attcttgac cggggtagc aacatgagt 
      tgaaatgaa gaaggttaa aaattctac cgatatcca 
      ccaaaagca gggccttga gcagtctaa aaagttgta 
      cagatctac aaccgagca ggcaaaacg tttagatga 
      cgctcgcag ctgcgcccg gaccatcag cgacctatt 
      cacccccaa cttgcaaat ccaacggcg gaaattctg 
      cgagttctg cgaacggtt gattttttc cgaccccgt 
      cctgtatgg aaaaaaagg agaggagtt actgtggcg 
      caaaggcta ttatgacgt cgtttccac cgcgaaaaa 
      actcaaaaa gaaggaata cacccagaa cgaaatttg 
      caatttact ggcacatgt taattccgg cattggctt 
      ctaccacca catgatatc caaactgca gtgcgaatt 
      taatttaaa tctgactca tgtcatcga tttttcaat 
      ggggggcgc cttcggcaa gggcgacgc cagaacgga 
      gtgtctcga gtgggcgaa cctttcagt acaccaatg 
      tttgattta tttaggcga tttgagtcg atacaaact 
      cgagattga ccccaaaat tggggcgac tctagcttt 
      ccgctatta cctacaagc catttcgtt caaacccga 
      ccacggcat gccccacaa tcaaatggg gactttagc 
      aatgaacca gaacaaatt tctagacgc ggtttacca 
      ctacatgcg ttgcctggc gtgtcctgt ttctttcga 
      cgggacaac gaataattt gcacagtct tttcgataa 
      aaactcgaa atatttaga gcaactccc ggtggagcc 
      aaactctga ttcgaacac gcaagacga tcatctcaa 
      acgatttat ttcgcggtt cctcctcct tatcgatat 
      ctggtactt taataaaaa agttgccaa agtttacag 
      tttgtgcca ccaaacgta caaagattt agaagagtg 
      tttgcaact gaagacaag caacgaaag ttgcgagtc 
      gccgaccgg gagcgaaac ggttgaaac cgactcagc 
      cgaacagtt gaacaatga catgatttt taaaatgat 
      cgtcatttg tctcctatg tatctccaa actgcagaa 
      aacaaacca gcatcaagt ctcaactgc tgttgcctt 
      cacccttgc catttattc ccagttggt tcaaaacgt 
      ggccaatca catccaaga aagtgtcct taattatcc 
      cttgccgga ggtggaggt taaaattga gcagaacgg 
      ggttgttaa agatctact gaagaagag taaaaagaa 
      gcgaaacgc gtctcagtt gcgtcgcag gaaagcact 
      tcctgtcga aatcgagtc ggatgatag cgaacgtga 
      agacgacga agtcgattc ggattcgga ttcctccga 
      ttcgtttgt ctctgactc ggagaatat tttggccga 
      atcgtccga cgatgagac tgaagttaa aaaatcgga 
      cgattcggt caatgacga taaaatcga cgtgattaa 
      gaaaatcaa acggatgga cgtgaacga catgctcga 
      gataataac agagcaagg ctccctcca agcgatcaa 
      aatcataaa cgactggtt gatgcaaga caccgaagt 
      tataaaatc ctgcggtac aagtactcg cagtctttt 
      acgtcaaat gacaaacat gctgaacct gattaatat 
      caatataaa aagcagcaa actatccgg tattaaatt 
      ggacgtgaa taatgttat aaaaaacga agagaaaat 
      cccgttgtc ggaagatat tgttttgaa aggtattga 
      catattgga gaaagctca caagaatat ggattggaa 
      ttatttaca taaacatgg aatgaacac gaaagaaga 
      gagcgtcac aaggatcgt aaaatttat ggcgtttgg 
      aaagtttct gaccggaat tgaggagac cgggatcac 
      ttttgacga acggaaaaa tgtttttgt ttgtaaatg 
      cgacgaaaa cgacgagaa taaagcgac caattcggt 
      cgtcgagga gttggaaat gcaagaaac cggccacgt 
      aatcggtga taaggaggg tgataaggg ccaacttat 
      gaaaatgaa acatatggg gcagttgtg gctggcgtc 
      tgaagtacg agcgttgga aaatagagt gaaaggaaa 
      ggcttcgct ctcgcccta tttggtttt agacgctga 
      cgccttaat taaatatac atttatggt caaacattt 
      cgtgcagtc gaggaaatt tatcgtctt aataccatc 
      tgcagttgt acaggcttt ggacgattt aaaacggga 
      gcagagtga agctcgtga cgctatccg ttggttgga 
      gagtcaatt tcatcgcgg aaaccgatt ttttcgggc 
      tcaacgacc ccaagaacg cgcggcgat tcctttcat 
      caagtatcc gaagaagcg agataagga aatgtcgac 
      gtacatcca gatcattga gtgttgcta ctacttggc 
      cgaacaaca gaaaggagc cgctaatct aattacttt 
      acttattgg gaatcagaa cgttttgtc gaacggcga 
      aaataagga atttagtta cgtgggctt ggcgcagtc 
      ggcagggat ttcgatcga atcaattac gcagtttta 
      tagtaaatg gaagaagag ttacaagac gaggagatg 
      aatggcgaa ttgagcggg atatgggat tatgagctc 
      atcggatcg tgcgggtgc aggcggtgg ttgtgaaga 
      attgttcat tttggaatt ttaaccgcg aactttatt 
      catttcaac aggatttgt agttttact tgttttgtc 
      agcaataaa ggcttattt tactg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY