Status
Please wait ...
Gene information
General information
TC001200 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 12570598..12574522
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001200
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_07893

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    mature egg not present - Number of animals showing the phenotype: 4
    vitellogenic egg chamber amount decreased - Number of animals showing the phenotype: 3
    follicular epithelium potentially structure irregular - Number of animals showing the phenotype: 3

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001200 RA
      MSAEHLKNI KLLCARIFQ NQRDIDSVR ELRNVLNRA 
      SGTIVKTLE PIIFPVFFP ILKSISESK NNYKENEKQ 
      EIVETFRSI FEKVKIENL ALFFNIYGF LLFEIYDHK 
      THTGTTISE LLNYSLLVL STSEEYKLS IVQCMTSLA 
      QNLSSSIIE ELYVKQNAP KLCQMIYVM LEMAKNEQF 
      KTLRIASIN CIMAIAHVL DDEDFDDIV IRNQIAEVF 
      LFFLPGVAT QLKSVALID EKFGHKIPS VALTAWGRI 
      VTLLMQDYN AQDRKIDLG GITDTTPTL NKKWENEAE 
      LEDYLKKTE RSPQWYKTT DKKLQMIII EFAKLTHHC 
      HPNVRLELA QMAALLLDN CVHTMPTSS SHLIDSVVV 
      LSEDGDDSV SRRSKQILE ELSVKLSRS EFKTLLDDL 
      EESFYTVVA SIPRKFNAI DERVQIVAL NSLIGYITL 
      FGRHNLTRV LYSSVHLNQ LLSTLIHIS EFDKSGFSV 
      LEEYTTLDY ELSHDSKMS WKKFRHFKE TEILDKIAT 
      VCRLLAKYG ASFIVSDFL LDVIATNEE QRKEAIFLL 
      NEVITGLGI ENNSTLFKN IITTYTDCD NWYLPLSVM 
      VDEYGYEHT LSDVQNNII QACLLVEGV GKIALALGN 
      EFQQFLLKT LYLVLERAG SSHPIIRTA GCSALANIS 
      TACQYSTIT DLINNNIDY FSFHIERKL SKAKGDKGG 
      LEVLGVVIR YSSQNVLIS TCDVIQELL YHTYDSHRT 
      NLNSYLQVF KLFIMGLHR WLGIEMPEE AFKTKKQKQ 
      DEFEHFQVS NLEPPIEDF SDEIMNKTA EEMYKEDLE 
      KSKKELEDE INEQPEEYK KPEPPLHIK LTKAILSRC 
      LHFLPSKNQ PRQLLVLEI LKHGVIILK NWEDELLPI 
      VHQIWGPLV DRFKDFNEP LIINYSFQL LVVLAKLSK 
      EFIRSRTAK EVLPNILTI LDKLSVESY LKDKGSAYR 
      YSQNYKLQV VILENLARV VTDLDMSQQ DTEKAIDVV 
      VKYVSDKQP IPLQNFALA FFKTVITYD PKSVETKVK 
      SLQISPENA KNVAQLVNF LEDLNK
    CDS sequence
    • >TC001200 RA
      atgagtgct gagcatttg aaaaatatt aagttactt 
      tgtgcacga atttttcaa aatcaaagg gatattgat 
      agtgtgagg gaattacgt aatgttcta aatagggct 
      agtggcaca attgtgaaa actttggaa ccgatcata 
      ttccctgtg ttttttccg atactgaaa agtatttca 
      gaatcaaag aacaattat aaagaaaat gagaagcaa 
      gaaatcgtt gagactttt cggtccatt tttgaaaaa 
      gttaaaatc gaaaatttg gctttgttt ttcaatatt 
      tacggattc cttttattc gaaatctac gaccataaa 
      acacacaca ggtacgacc atatctgaa ctacttaat 
      tactctctc ttagtttta agcacaagt gaggagtac 
      aaattaagt atagtacaa tgtatgaca tcgttagcc 
      caaaactta agttcttca ataatagaa gaattgtac 
      gtgaagcaa aacgcccca aaactgtgc caaatgatt 
      tacgttatg ttagaaatg gcaaaaaat gagcagttc 
      aaaactttg cggattgcg tccataaat tgcataatg 
      gcaatcgcc cacgttctg gacgacgaa gatttcgac 
      gatatcgtc attaggaac caaatcgcc gaagttttt 
      ctctttttt ctgccaggc gtcgcaact cagcttaaa 
      tcagtcgcg ctaattgat gagaaattc ggtcacaaa 
      atcccttcg gtagccctc acagcttgg ggaaggatc 
      gtgactttg ctaatgcag gactacaat gcacaggac 
      cgtaaaatc gatctcgga ggaatcaca gacacaacc 
      cccactctt aacaaaaaa tgggaaaac gaggcggag 
      ttagaggac tatcttaaa aaaacggaa cgctcgcct 
      cagtggtac aaaaccact gataagaaa ctacaaatg 
      ataattatc gagtttgcg aagctgacc catcattgt 
      cacccaaat gttcggcta gaacttgca caaatggca 
      gctttgctt ttggacaat tgcgtccac actatgcca 
      acatcaagc agtcattta atcgatagt gttgttgtt 
      ctatcggaa gatggtgat gattcggtt tcgaggaga 
      agtaagcag attttggag gaactatca gtcaaactc 
      tcaaggagc gaatttaaa actttgctt gacgatttg 
      gaggagagt ttttacact gttgttgcc agtattcct 
      cgcaagttt aacgcaata gatgagagg gtacaaatc 
      gttgctttg aactcgctg atcggttac ataactctc 
      ttcggtcgg cataacttg actcgagtg ctctactca 
      tcggttcat ttgaaccaa ctcttgtca actcttatc 
      cacatatcg gagtttgac aaaagtggg ttcagtgtc 
      ttagaagag tatacaact ttagactat gagttaagt 
      cacgactcg aaaatgtcg tggaaaaaa ttccgacac 
      tttaaagaa accgaaatc ttggacaaa attgcaact 
      gtttgtcga cttttggcc aaatacggt gcctcattc 
      attgttagc gattttcta ttggacgtt atagccacc 
      aatgaggaa caacgaaaa gaggcgatt tttttacta 
      aatgaagtg ataacaggt ttaggaatt gaaaataat 
      tcaacttta ttcaaaaat ataatcaca acatacact 
      gattgtgac aattggtac ttacctttg tcggtaatg 
      gtggatgag tacggatac gaacacact ttaagtgac 
      gtacaaaat aatataatt caagcttgt ttattagtc 
      gagggggtt ggaaaaatc gccttggct ctagggaat 
      gagtttcag caattttta ttaaagact ttatatctg 
      gtgttagaa cgagcaggc agttcccac ccgattatc 
      cgaactgcg ggttgttcg gccctcgct aacattagt 
      acagcttgt cagtattcg acaataacc gacttaatt 
      aacaacaat attgactat ttctcgttc cacattgag 
      cgcaaattg agtaaagcc aagggtgat aagggcggc 
      ttggaggtg ctaggagtc gtgataaga tacagttct 
      caaaacgtt ttaatttca acctgtgat gttattcaa 
      gaattattg tatcacacg tacgacagc catcggacc 
      aatttaaat tcatacttg caagttttt aagttattt 
      ataatgggg ttacaccgg tggttaggc attgaaatg 
      cccgaggag gcatttaag acaaaaaag caaaaacag 
      gacgaattc gaacacttt caagtttcg aatctcgaa 
      ccacctatt gaggatttt tcggacgaa attatgaac 
      aaaacggcc gaagaaatg tacaaagaa gacttggag 
      aagagcaag aaagagttg gaggacgaa attaacgaa 
      caaccagaa gagtacaaa aagcccgag cccccactt 
      cacatcaaa ctaacaaaa gcgattttg agccgatgt 
      ttacacttc ctgccttcc aaaaaccaa ccccgacaa 
      ttacttgtt ttggaaatt ttgaaacac ggtgtaatt 
      attttgaaa aactgggag gatgagtta ctaccaatc 
      gtgcaccag atttggggg cctttagtc gaccgattt 
      aaggatttt aatgaaccg ttaatcatc aattacagt 
      tttcaactt cttgtcgtg cttgcgaaa ttatcgaaa 
      gagttcata cggtcccgg acggcaaag gaagtttta 
      ccaaacatt ttaacaatt cttgataaa ttatcggtc 
      gagagttat ttaaaagat aagggatcg gcctaccgt 
      tacagccaa aattacaaa ttacaagtg gtaattttg 
      gagaatttg gcccgagtt gtgaccgac ttggacatg 
      tctcaacag gacaccgaa aaagccatt gatgttgtt 
      gttaaatac gtcagtgat aagcaaccg attcctcta 
      cagaatttt gcactagct tttttcaaa acggtaata 
      acatatgac ccaaagtca gtcgagacg aaagtcaaa 
      tcgttgcaa atttcgccc gaaaatgcg aaaaacgta 
      gcacaactt gtgaatttt ttagaggat ttaaataaa 
      taa
    mRNA sequence
    • >TC001200 RA
      caccaaaac aacaattct atgactaac tgcaccttc 
      aaattgcaa aaattaggc aatttttgg acttaaaac 
      aaccaattt tgggcatat ttacacaat tcatgtgaa 
      attctctta atattttta ataataaca cggttttta 
      gtagcaatt tcaagttta ccgccgcat gtggcggct 
      gcggcaacg catgacggt aacagagac gcaaaaacg 
      cgattaaat caatatctg gcggtgatt taagggttt 
      tttataatg ccacgtacc tgaattcag tggagagtt 
      aatctgtta cttcgatac aaacaaagg ttatattgg 
      tactactgg ttacccaac gtggggaat tcaaaatga 
      gtgctgagc atttgaaaa atattaagt tactttgtg 
      cacgaattt ttcaaaatc aaagggata ttgatagtg 
      tgagggaat tacgtaatg ttctaaata gggctagtg 
      gcacaattg tgaaaactt tggaaccga tcatattcc 
      ctgtgtttt ttccgatac tgaaaagta tttcagaat 
      caaagaaca attataaag aaaatgaga agcaagaaa 
      tcgttgaga cttttcggt ccatttttg aaaaagtta 
      aaatcgaaa atttggctt tgtttttca atatttacg 
      gattccttt tattcgaaa tctacgacc ataaaacac 
      acacaggta cgaccatat ctgaactac ttaattact 
      ctctcttag ttttaagca caagtgagg agtacaaat 
      taagtatag tacaatgta tgacatcgt tagcccaaa 
      acttaagtt cttcaataa tagaagaat tgtacgtga 
      agcaaaacg ccccaaaac tgtgccaaa tgatttacg 
      ttatgttag aaatggcaa aaaatgagc agttcaaaa 
      ctttgcgga ttgcgtcca taaattgca taatggcaa 
      tcgcccacg ttctggacg acgaagatt tcgacgata 
      tcgtcatta ggaaccaaa tcgccgaag tttttctct 
      tttttctgc caggcgtcg caactcagc ttaaatcag 
      tcgcgctaa ttgatgaga aattcggtc acaaaatcc 
      cttcggtag ccctcacag cttggggaa ggatcgtga 
      ctttgctaa tgcaggact acaatgcac aggaccgta 
      aaatcgatc tcggaggaa tcacagaca caaccccca 
      ctcttaaca aaaaatggg aaaacgagg cggagttag 
      aggactatc ttaaaaaaa cggaacgct cgcctcagt 
      ggtacaaaa ccactgata agaaactac aaatgataa 
      ttatcgagt ttgcgaagc tgacccatc attgtcacc 
      caaatgttc ggctagaac ttgcacaaa tggcagctt 
      tgcttttgg acaattgcg tccacacta tgccaacat 
      caagcagtc atttaatcg atagtgttg ttgttctat 
      cggaagatg gtgatgatt cggtttcga ggagaagta 
      agcagattt tggaggaac tatcagtca aactctcaa 
      ggagcgaat ttaaaactt tgcttgacg atttggagg 
      agagttttt acactgttg ttgccagta ttcctcgca 
      agtttaacg caatagatg agagggtac aaatcgttg 
      ctttgaact cgctgatcg gttacataa ctctcttcg 
      gtcggcata acttgactc gagtgctct actcatcgg 
      ttcatttga accaactct tgtcaactc ttatccaca 
      tatcggagt ttgacaaaa gtgggttca gtgtcttag 
      aagagtata caactttag actatgagt taagtcacg 
      actcgaaaa tgtcgtgga aaaaattcc gacacttta 
      aagaaaccg aaatcttgg acaaaattg caactgttt 
      gtcgacttt tggccaaat acggtgcct cattcattg 
      ttagcgatt ttctattgg acgttatag ccaccaatg 
      aggaacaac gaaaagagg cgatttttt tactaaatg 
      aagtgataa caggtttag gaattgaaa ataattcaa 
      ctttattca aaaatataa tcacaacat acactgatt 
      gtgacaatt ggtacttac ctttgtcgg taatggtgg 
      atgagtacg gatacgaac acactttaa gtgacgtac 
      aaaataata taattcaag cttgtttat tagtcgagg 
      gggttggaa aaatcgcct tggctctag ggaatgagt 
      ttcagcaat ttttattaa agactttat atctggtgt 
      tagaacgag caggcagtt cccacccga ttatccgaa 
      ctgcgggtt gttcggccc tcgctaaca ttagtacag 
      cttgtcagt attcgacaa taaccgact taattaaca 
      acaatattg actatttct cgttccaca ttgagcgca 
      aattgagta aagccaagg gtgataagg gcggcttgg 
      aggtgctag gagtcgtga taagataca gttctcaaa 
      acgttttaa tttcaacct gtgatgtta ttcaagaat 
      tattgtatc acacgtacg acagccatc ggaccaatt 
      taaattcat acttgcaag tttttaagt tatttataa 
      tggggttac accggtggt taggcattg aaatgcccg 
      aggaggcat ttaagacaa aaaagcaaa aacaggacg 
      aattcgaac actttcaag tttcgaatc tcgaaccac 
      ctattgagg atttttcgg acgaaatta tgaacaaaa 
      cggccgaag aaatgtaca aagaagact tggagaaga 
      gcaagaaag agttggagg acgaaatta acgaacaac 
      cagaagagt acaaaaagc ccgagcccc cacttcaca 
      tcaaactaa caaaagcga ttttgagcc gatgtttac 
      acttcctgc cttccaaaa accaacccc gacaattac 
      ttgttttgg aaattttga aacacggtg taattattt 
      tgaaaaact gggaggatg agttactac caatcgtgc 
      accagattt gggggcctt tagtcgacc gatttaagg 
      attttaatg aaccgttaa tcatcaatt acagttttc 
      aacttcttg tcgtgcttg cgaaattat cgaaagagt 
      tcatacggt cccggacgg caaaggaag ttttaccaa 
      acattttaa caattcttg ataaattat cggtcgaga 
      gttatttaa aagataagg gatcggcct accgttaca 
      gccaaaatt acaaattac aagtggtaa ttttggaga 
      atttggccc gagttgtga ccgacttgg acatgtctc 
      aacaggaca ccgaaaaag ccattgatg ttgttgtta 
      aatacgtca gtgataagc aaccgattc ctctacaga 
      attttgcac tagcttttt tcaaaacgg taataacat 
      atgacccaa agtcagtcg agacgaaag tcaaatcgt 
      tgcaaattt cgcccgaaa atgcgaaaa acgtagcac 
      aacttgtga attttttag aggatttaa ataaataaa 
      acgtttaca aagatttgt tttatttgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY