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Gene information
General information
TC011067 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 5562117..5597973
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011067
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_07888

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    larva mostly everted (inside-out) - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    antenna potentially fused - Penetrance (percentage of animals/eggs on slide): less than 30%

    labrum not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    head partially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    antenna present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    head fragmented - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    thorax fragmented - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    thorax everted (inside-out) - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen everted (inside-out) - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011067 RA
      MLLFILILV LPLLTTSRY HRFQYKPRN IQCHCVPQH 
      LCTDEDVTI NGHGLLDPR IGNMICSNP SEICCDSPP 
      KPESPEIPR CGFSATFKS RITSNTMAQ FGELPWNLI 
      IQESSGEDR NIYKCGGSL IHPRVALTA AHCVAPYSE 
      QPEKILVRA GEWNIDSRD EILPFQDNS VEEILIHYD 
      YSSLSLKND IAILILVED FVLRDNVKT LCLPSPDVK 
      VVENGCLAS GWGKNAHTK GRYSSTLKK VQVPIVPRS 
      HCRRTIAYH RASVRCVCV LLGASLCAV CDLNSDKPE 
      FGLNMRHLQ HSN
    CDS sequence
    • >TC011067 RA
      atgcttctc ttcatcctc atactcgta ctacccctt 
      ttgacaacc tctcgatat catcgtttt caatacaaa 
      ccacgaaat atccagtgc cattgtgtc ccgcaacat 
      ctttgtaca gacgaggac gtgacaatc aacggacat 
      ggattattg gatccccgg atcggcaac atgatttgc 
      tccaacccg tccgaaatc tgctgtgat tccccccca 
      aaaccggaa agccccgaa attccccga tgcggcttt 
      tccgccact ttcaaatcc cgaataacc tcaaacacg 
      atggcgcag ttcggggaa ttaccgtgg aatttaatc 
      attcaggag agttctgga gaagaccgc aacatttac 
      aaatgcggg gggtcccta atccacccc cgggtggcc 
      ctcacagcg gcccattgt gtggcccct tactcggaa 
      caaccggag aaaatcctg gtccgggcc ggggaatgg 
      aacatcgac tcccgggac gaaattctc cctttccag 
      gacaattcc gtcgaagaa atcctcatc cattacgac 
      tacagttct ctctcgttg aaaaacgac atcgccatt 
      ctgatcctg gtcgaggat ttcgtcctg agggacaac 
      gtcaagacc ttgtgtctc ccttcgcca gatgtcaaa 
      gtggtggaa aatgggtgt ttggcgagc gggtggggg 
      aaaaacgcc catacgaaa ggtcgctac tcctcgacc 
      ttgaaaaag gtccaagtg cccattgtt ccaaggagt 
      cattgccgc cgtaccatc gcctaccat cgtgccagt 
      gttcggtgt gtgtgtgtt ttgctgggc gccagcctt 
      tgtgcagtg tgcgattta aattcggat aaaccggaa 
      ttcgggtta aacatgcga catttgcaa cactcgaac 
      taa
    mRNA sequence
    • >TC011067 RA
      atcaagtcg tcaaaccgc aacaatgct tctcttcat 
      cctcatact cgtactacc ccttttgac aacctctcg 
      atatcatcg ttttcaata caaaccacg aaatatcca 
      gtgccattg tgtcccgca acatctttg tacagacga 
      ggacgtgac aatcaacgg acatggatt attggatcc 
      ccggatcgg caacatgat ttgctccaa cccgtccga 
      aatctgctg tgattcccc cccaaaacc ggaaagccc 
      cgaaattcc ccgatgcgg cttttccgc cactttcaa 
      atcccgaat aacctcaaa cacgatggc gcagttcgg 
      ggaattacc gtggaattt aatcattca ggagagttc 
      tggagaaga ccgcaacat ttacaaatg cggggggtc 
      cctaatcca cccccgggt ggccctcac agcggccca 
      ttgtgtggc cccttactc ggaacaacc ggagaaaat 
      cctggtccg ggccgggga atggaacat cgactcccg 
      ggacgaaat tctcccttt ccaggacaa ttccgtcga 
      agaaatcct catccatta cgactacag ttctctctc 
      gttgaaaaa cgacatcgc cattctgat cctggtcga 
      ggatttcgt cctgaggga caacgtcaa gaccttgtg 
      tctcccttc gccagatgt caaagtggt ggaaaatgg 
      gtgtttggc gagcgggtg ggggaaaaa cgcccatac 
      gaaaggtcg ctactcctc gaccttgaa aaaggtcca 
      agtgcccat tgttccaag gagtcattg ccgccgtac 
      catcgccta ccatcgtgc cagtgttcg gtgtgtgtg 
      tgttttgct gggcgccag cctttgtgc agtgtgcga 
      tttaaattc ggataaacc ggaattcgg gttaaacat 
      gcgacattt gcaacactc gaactaaac ccattgagc 
      tgaatttga aaaggtgag tttgtggtt gactgctcg 
      gccttggct tcgttttgc caaaaaatg ggcttgaca 
      aagccttgt cttgccaaa atttaaaaa tcgaaaata 
      cctaccaca ggataattt gcagatgtg tgtaattaa 
      agacgtgat attttgtaa taaaaacta atggttgct
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY