General information | |||
TC032174
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 11152852..11175574 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
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TC032174 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_07879 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC032174 RA
MERMESLSD PALCLQDLV TGVTTSSAN SDMHGVHHL HDSVSSAVS VSSAITGIM SGASASVLG HHVTSHEPP HHHGVVPHT PSLHHEPLE KLKRVWAET GDFRDAHSG MSGSTMDHP QLPFPTAAR NSRTRDRKG SRSLSDPIK TESGVGVDS TDGDDGKND KKTKRQRRQ RTHFTSQQL QELEATFAR NRYPDMSTR EEIAMWTNL TEARVRVWF KNRRAKWRK RERNAMNAM NAAAEFKTG FGTQFNGLM PTFGDTDTL YSSYSYNNW ATKVPSPLG TKPFWSVVP PNHHQSPVN CFNAATSVA AASMSGAAG TMLPGGMSS GLTSTPTAV APQPCPYTT PANPYSMYH HRAATEPCS AMSSSIASL RLKAKQHTG FVGYSSVSP VRSSSAGLS ACQYAGSGI GVGERPG
- >TC032174 RA
atggagcgg atggaatcg ctgtctgat cccgcattg tgcctccaa gacctagtg acgggcgtg acaacatcc tcggcgaac tccgacatg cacggggtc caccacctg cacgactcc gtcagctcg gccgtttcg gtgagctca gcgatcacc ggaatcatg agcggggct tcggcctcc gtgctgggc caccacgtc acctcgcac gagccgccg caccaccac ggagtggtg ccccacacg ccgtccctc caccacgag cccttggag aagctgaaa cgtgtttgg gccgagacc ggagatttc cgcgacgcc cattccgga atgtcagga tctaccatg gaccaccct caactgcct tttcccacc gccgcgagg aacagtcga acgagagac cggaaaggc agcagatcg ttgtccgac cccatcaaa acagagtcg ggggtaggt gtggattcg acggatggc gacgatggg aaaaatgac aagaaaacg aaacggcag cggaggcaa agaacacac ttcactagt cagcagctg caagagctg gaagcgact tttgcccgg aatcggtat ccggacatg agtaccaga gaagagatt gcgatgtgg acgaatttg accgaagcg agagtgagg gtgtggttc aaaaaccgc cgcgccaag tggcggaaa cgcgaacgc aacgccatg aacgcaatg aacgccgcc gccgagttc aaaactggc ttcgggacg caattcaac ggcttgatg cccactttc ggcgacacc gacaccctc tactcctcc tacagctac aacaactgg gcgacgaag gtgcccagc cccctcggc accaagccc ttctggtcg gtggtgccg cccaaccac caccagagc cccgtgaac tgcttcaac gccgccacg tcggtggcg gcggcctcc atgtcgggg gcggcgggc accatgctc cccggcggc atgagctcg gggctgacc agcactccg acggccgtg gccccccag ccgtgcccc tacaccacc cccgccaac ccctactcc atgtaccac cacagggct gcgacggag ccctgcagc gccatgtcc tccagcata gcatcgctg aggctcaag gcgaagcag cacactggc tttgtgggg tattcgagt gttagtccg gtgaggtcg tcttcggca gggctgtca gcgtgccag tatgcggga agcgggata ggggtgggt gagaggcct ggctga
- >TC032174 RA
cgcgttggg aagtggccc agattacag agactttgc agtatcaag tcctaaccc gatatctgt acggccgct aagcgctta agacttttt aatactttc ataggattt cgtgaaaac cgccctgca tttgcagtg gcagtgatt tgtaagata ttaacggcg cggacaaac ggtttcatg acaaggatt ttcgagtgg atgatcgcg gatttattt atgtttttc cggtgtttt attgagatt ttcgggagt gctgaaagg actgtgata gatttgtga ttcgacgat ttcgtcctg gacatcgca cgaggattc taacaggtg gcacacatc agcggcgat gttatcgac ctagacgtc gtgcagtaa accctcgaa aaaataatt tgatcgcgc gcgcgtgca ttccaactt aaatcctgt aataactca aatggagcg gatggaatc gctgtctga tcccgcatt gtgcctcca agacctagt gacgggcgt gacaacatc ctcggcgaa ctccgacat gcacggggt ccaccacct gcacgactc cgtcagctc ggccgtttc ggtgagctc agcgatcac cggaatcat gagcggggc ttcggcctc cgtgctggg ccaccacgt cacctcgca cgagccgcc gcaccacca cggagtggt gccccacac gccgtccct ccaccacga gcccttgga gaagctgaa acgtgtttg ggccgagac cggagattt ccgcgacgc ccattccgg aatgtcagg atctaccat ggaccaccc tcaactgcc ttttcccac cgccgcgag gaacagtcg aacgagaga ccggaaagg cagcagatc gttgtccga ccccatcaa aacagagtc gggggtagg tgtggattc gacggatgg cgacgatgg gaaaaatga caagaaaac gaaacggca gcggaggca aagaacaca cttcactag tcagcagct gcaagagct ggaagcgac ttttgcccg gaatcggta tccggacat gagtaccag agaagagat tgcgatgtg gacgaattt gaccgaagc gagagtgag ggtgtggtt caaaaaccg ccgcgccaa gtggcggaa acgcgaacg caacgccat gaacgcaat gaacgccgc cgccgagtt caaaactgg cttcgggac gcaattcaa cggcttgat gcccacttt cggcgacac cgacaccct ctactcctc ctacagcta caacaactg ggcgacgaa ggtgcccag ccccctcgg caccaagcc cttctggtc ggtggtgcc gcccaacca ccaccagag ccccgtgaa ctgcttcaa cgccgccac gtcggtggc ggcggcctc catgtcggg ggcggcggg caccatgct ccccggcgg catgagctc ggggctgac cagcactcc gacggccgt ggcccccca gccgtgccc ctacaccac ccccgccaa cccctactc catgtacca ccacagggc tgcgacgga gccctgcag cgccatgtc ctccagcat agcatcgct gaggctcaa ggcgaagca gcacactgg ctttgtggg gtattcgag tgttagtcc ggtgaggtc gtcttcggc agggctgtc agcgtgcca gtatgcggg aagcgggat aggggtggg tgagaggcc tggctgaga gcacgatct ggccttggt ggcccaatt aaaacacga agaccaaga cgattgata agataagta cggttaaac aaacacgaa gttaggtgt ttgcgaatg ataaggggg taaaatggc aggcggatt ccattacgc ggatatttc gccaaagaa ttcgctaat ttgctaagg cgcaattaa ttcagaaca tattgtttt aaacgaggc agcaattta ataaagcgc gatacaatg aatcaaatt aaaattttc aaatgagtg caaatgtga aaaatttgc tttgaatat ttaagtacc aaaaaagac cgatttctt gaaaactaa ctgagaagt tcttcacta aatatgaaa gaccagtgg ttcgttggt tcttcagtt tgttaatta tctttacga tctttaagc tttaaaaat ttttttctt ttgctcttt taaaatatt tttaatgcc gctgtacct cgttttcag ttcgtttaa actacaagt tagaccatt cgaattttt gtgtttgtt actataaat aacgagttt tttttataa gtattttat tgttatact gcagttaga aaattgtta atcaacaag gtttttgtt accaatcat tgtagaaaa atttatttt ttatttttt tataatgta cataactcc tttctgcag taaagcatt cagctgcct cacaaaaat gagatcatt ttggtaatt ttattaaat aaagtataa ctttacac
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
