Status
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Gene information
General information
TC032174
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC001112
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 11152852..11175574
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032174
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_07879

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032174 RA
      MERMESLSD PALCLQDLV TGVTTSSAN SDMHGVHHL 
      HDSVSSAVS VSSAITGIM SGASASVLG HHVTSHEPP 
      HHHGVVPHT PSLHHEPLE KLKRVWAET GDFRDAHSG 
      MSGSTMDHP QLPFPTAAR NSRTRDRKG SRSLSDPIK 
      TESGVGVDS TDGDDGKND KKTKRQRRQ RTHFTSQQL 
      QELEATFAR NRYPDMSTR EEIAMWTNL TEARVRVWF 
      KNRRAKWRK RERNAMNAM NAAAEFKTG FGTQFNGLM 
      PTFGDTDTL YSSYSYNNW ATKVPSPLG TKPFWSVVP 
      PNHHQSPVN CFNAATSVA AASMSGAAG TMLPGGMSS 
      GLTSTPTAV APQPCPYTT PANPYSMYH HRAATEPCS 
      AMSSSIASL RLKAKQHTG FVGYSSVSP VRSSSAGLS 
      ACQYAGSGI GVGERPG
    CDS sequence
    • >TC032174 RA
      atggagcgg atggaatcg ctgtctgat cccgcattg 
      tgcctccaa gacctagtg acgggcgtg acaacatcc 
      tcggcgaac tccgacatg cacggggtc caccacctg 
      cacgactcc gtcagctcg gccgtttcg gtgagctca 
      gcgatcacc ggaatcatg agcggggct tcggcctcc 
      gtgctgggc caccacgtc acctcgcac gagccgccg 
      caccaccac ggagtggtg ccccacacg ccgtccctc 
      caccacgag cccttggag aagctgaaa cgtgtttgg 
      gccgagacc ggagatttc cgcgacgcc cattccgga 
      atgtcagga tctaccatg gaccaccct caactgcct 
      tttcccacc gccgcgagg aacagtcga acgagagac 
      cggaaaggc agcagatcg ttgtccgac cccatcaaa 
      acagagtcg ggggtaggt gtggattcg acggatggc 
      gacgatggg aaaaatgac aagaaaacg aaacggcag 
      cggaggcaa agaacacac ttcactagt cagcagctg 
      caagagctg gaagcgact tttgcccgg aatcggtat 
      ccggacatg agtaccaga gaagagatt gcgatgtgg 
      acgaatttg accgaagcg agagtgagg gtgtggttc 
      aaaaaccgc cgcgccaag tggcggaaa cgcgaacgc 
      aacgccatg aacgcaatg aacgccgcc gccgagttc 
      aaaactggc ttcgggacg caattcaac ggcttgatg 
      cccactttc ggcgacacc gacaccctc tactcctcc 
      tacagctac aacaactgg gcgacgaag gtgcccagc 
      cccctcggc accaagccc ttctggtcg gtggtgccg 
      cccaaccac caccagagc cccgtgaac tgcttcaac 
      gccgccacg tcggtggcg gcggcctcc atgtcgggg 
      gcggcgggc accatgctc cccggcggc atgagctcg 
      gggctgacc agcactccg acggccgtg gccccccag 
      ccgtgcccc tacaccacc cccgccaac ccctactcc 
      atgtaccac cacagggct gcgacggag ccctgcagc 
      gccatgtcc tccagcata gcatcgctg aggctcaag 
      gcgaagcag cacactggc tttgtgggg tattcgagt 
      gttagtccg gtgaggtcg tcttcggca gggctgtca 
      gcgtgccag tatgcggga agcgggata ggggtgggt 
      gagaggcct ggctga
    mRNA sequence
    • >TC032174 RA
      cgcgttggg aagtggccc agattacag agactttgc 
      agtatcaag tcctaaccc gatatctgt acggccgct 
      aagcgctta agacttttt aatactttc ataggattt 
      cgtgaaaac cgccctgca tttgcagtg gcagtgatt 
      tgtaagata ttaacggcg cggacaaac ggtttcatg 
      acaaggatt ttcgagtgg atgatcgcg gatttattt 
      atgtttttc cggtgtttt attgagatt ttcgggagt 
      gctgaaagg actgtgata gatttgtga ttcgacgat 
      ttcgtcctg gacatcgca cgaggattc taacaggtg 
      gcacacatc agcggcgat gttatcgac ctagacgtc 
      gtgcagtaa accctcgaa aaaataatt tgatcgcgc 
      gcgcgtgca ttccaactt aaatcctgt aataactca 
      aatggagcg gatggaatc gctgtctga tcccgcatt 
      gtgcctcca agacctagt gacgggcgt gacaacatc 
      ctcggcgaa ctccgacat gcacggggt ccaccacct 
      gcacgactc cgtcagctc ggccgtttc ggtgagctc 
      agcgatcac cggaatcat gagcggggc ttcggcctc 
      cgtgctggg ccaccacgt cacctcgca cgagccgcc 
      gcaccacca cggagtggt gccccacac gccgtccct 
      ccaccacga gcccttgga gaagctgaa acgtgtttg 
      ggccgagac cggagattt ccgcgacgc ccattccgg 
      aatgtcagg atctaccat ggaccaccc tcaactgcc 
      ttttcccac cgccgcgag gaacagtcg aacgagaga 
      ccggaaagg cagcagatc gttgtccga ccccatcaa 
      aacagagtc gggggtagg tgtggattc gacggatgg 
      cgacgatgg gaaaaatga caagaaaac gaaacggca 
      gcggaggca aagaacaca cttcactag tcagcagct 
      gcaagagct ggaagcgac ttttgcccg gaatcggta 
      tccggacat gagtaccag agaagagat tgcgatgtg 
      gacgaattt gaccgaagc gagagtgag ggtgtggtt 
      caaaaaccg ccgcgccaa gtggcggaa acgcgaacg 
      caacgccat gaacgcaat gaacgccgc cgccgagtt 
      caaaactgg cttcgggac gcaattcaa cggcttgat 
      gcccacttt cggcgacac cgacaccct ctactcctc 
      ctacagcta caacaactg ggcgacgaa ggtgcccag 
      ccccctcgg caccaagcc cttctggtc ggtggtgcc 
      gcccaacca ccaccagag ccccgtgaa ctgcttcaa 
      cgccgccac gtcggtggc ggcggcctc catgtcggg 
      ggcggcggg caccatgct ccccggcgg catgagctc 
      ggggctgac cagcactcc gacggccgt ggcccccca 
      gccgtgccc ctacaccac ccccgccaa cccctactc 
      catgtacca ccacagggc tgcgacgga gccctgcag 
      cgccatgtc ctccagcat agcatcgct gaggctcaa 
      ggcgaagca gcacactgg ctttgtggg gtattcgag 
      tgttagtcc ggtgaggtc gtcttcggc agggctgtc 
      agcgtgcca gtatgcggg aagcgggat aggggtggg 
      tgagaggcc tggctgaga gcacgatct ggccttggt 
      ggcccaatt aaaacacga agaccaaga cgattgata 
      agataagta cggttaaac aaacacgaa gttaggtgt 
      ttgcgaatg ataaggggg taaaatggc aggcggatt 
      ccattacgc ggatatttc gccaaagaa ttcgctaat 
      ttgctaagg cgcaattaa ttcagaaca tattgtttt 
      aaacgaggc agcaattta ataaagcgc gatacaatg 
      aatcaaatt aaaattttc aaatgagtg caaatgtga 
      aaaatttgc tttgaatat ttaagtacc aaaaaagac 
      cgatttctt gaaaactaa ctgagaagt tcttcacta 
      aatatgaaa gaccagtgg ttcgttggt tcttcagtt 
      tgttaatta tctttacga tctttaagc tttaaaaat 
      ttttttctt ttgctcttt taaaatatt tttaatgcc 
      gctgtacct cgttttcag ttcgtttaa actacaagt 
      tagaccatt cgaattttt gtgtttgtt actataaat 
      aacgagttt tttttataa gtattttat tgttatact 
      gcagttaga aaattgtta atcaacaag gtttttgtt 
      accaatcat tgtagaaaa atttatttt ttatttttt 
      tataatgta cataactcc tttctgcag taaagcatt 
      cagctgcct cacaaaaat gagatcatt ttggtaatt 
      ttattaaat aaagtataa ctttacac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY