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Gene information
General information
TC001108 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 11056172..11062496
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001108
    iBeetle screen iB_07878

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    yolk late partiallyamount decreased - Number of animals showing the phenotype: 3

    mature egg not present - Number of animals showing the phenotype: 2
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001108 RA
      MDEEDELKP RNYQVNLME IAIRENTII YLPTGSGKT 
      FIAIMVLKQ LCAPILRPY SDGGKISVI LVNSVALVD 
      QHGKYVRDH ATFSVGTYT GEMNVDFWS EAEWEQQFN 
      KYQVVIMTS QIMVNLINN RFIDLGKVN LMIFDECHH 
      GVEDQPMRQ IMKHFHSCT DKPRVLGLT ATLLNGNCK 
      LSKVMDEIR SLEVTFHSK VATVEGLDV VVGYSTNPQ 
      ELFKVCQPG ALSLDAKQV LNNLRQLIN DLEHINIKD 
      EQNSVNLLQ SETLKPLEP SDVLKSLRN LISDLMIHI 
      EMLGAFGGH IACVAHMIQ IERIKKHCQ NHQLFIVLN 
      YVMTIMGTT KLLLEETMA GYEPLEKIR KFSSDKVLK 
      VFEILDEYK TKSDEELCC LVFTKRRFT AKVLHHIID 
      KASQVDPKF YHIKSNFVV GNKNNPYND TRENLYITK 
      KNREVLNSF VSKEINVLV SSNVLEEGV DIPKCTLVI 
      KFDKSEDYR SYIQSKGRA RHIKSLYYT IVETTDVAK 
      YDKKYSAFK EIENLVNDL LIGKNSERD HPNLSEIRN 
      MYNEDKLEP YYVNGPNSA QVNMTSAVA LLCRYCSNL 
      ASDKYTTYA PEWYYEEDS SSAKLRVVI FLPVVCPLI 
      DPIVGPYMH NKKDAKRAA ALVACIKLH QCGELDNNL 
      LPWKKQLDE ADVSYLFTH WPQEKESDA GNKKKKRLH 
      DKEIAPSVK SAIQPDRVL YLHTININP QYKRSDDLK 
      NAVTIYDLY KTPLKFGLL SPKPLPDLC KFPLFDSNG 
      TLEIEIRNN VREVEFAAN EMKEMREFH FLVFNDLLE 
      ILKEFLIFD NTGMNSEML LVVPVQDRC GDVCVDFRV 
      IRDNKNLKN KLEPAATER INLNVTEET YLHKIVSPW 
      YRSPPKMYV VTKVCPDKS ALSRFPNHE YPNFVSYYS 
      EKHSLSILD PSQPLLLVK GLSERLNAF KPRGAGGKR 
      KKEKMYEEL EEYLIPELV IKQEFPSCL WIQARFLPS 
      ILSRLAYLL KLQQLQVDI ARGIGAKAE YLKDCPPLE 
      LNLHLLHYE PNDPQLTQE SDKSTPLID NCLALECPK 
      NLRTIQYNK DFAAKMLEA EYYWKTIEE PKDIERNIN 
      VTVMDIEYY ETFISHQPS KTGRLLKND SPVKQQNVP 
      AITYDCQFE AKQLQILDV QFDNQSPNL CQIYQALTA 
      AEANDIVNL ERLETLGDS FLKFVASLY IIFKFPTYN 
      EGKSTTLKG KLVSNKNLY YLGVRKNLG GILKNSDLS 
      PSDWVPPCF CIPQTISKA IGNKEYSVV SLFNCCISP 
      EEQVSGNLN RKTLSDMTT EEIAPDEEN SYGNMCNFL 
      NKQYVGDKS IADSVEALL GAYFLSGGI QGGIKFMEW 
      IGILPLSEQ IQRLIETTQ VDPVLNKKS TKTDVDFHM 
      PQWREIEQR LGYTFTNRA FLLQALTHS SYSPNRITL 
      SYERLEFLG DAVLDFLIT CYIFEHCGH LEPGQVTDL 
      RSSLVNNNT FASLVVRCG FHKFLLMMN SNLQGHIDK 
      FADYLASKN YVIDDEVLI LLEEDEMNI AEYVDVPKV 
      LGDIFEALA GAIYLDSNK DLKTVWRVF YKIIWREID 
      LFSKNVPKN VIRRLYECH TVYPPQFSK ALEVGNQKT 
      MVSLDFMCE GRKKRVHGF GTNKILAKR AAAKIALRA 
      LKL
    CDS sequence
    • >TC001108 RA
      atggacgaa gaagacgaa cttaaacct cgtaactac 
      caagtcaat ttgatggaa attgccatt cgggaaaac 
      acaattatt tatctaccg actggatcg ggtaaaact 
      tttattgca ataatggtt ttgaagcag ttgtgcgcc 
      cccatattg agaccctac agcgatggt ggaaagatt 
      tcagttata ttggttaat tctgtggct ttggtggat 
      caacatggt aagtatgta agagaccac gccacgttc 
      agtgtggga acttacact ggagagatg aatgtggat 
      ttttggagc gaggcagaa tgggaacag caatttaat 
      aagtatcaa gttgttata atgaccagc caaatcatg 
      gtaaatctc atcaacaac cgcttcata gatttggga 
      aaagtaaac ttgatgatt ttcgatgaa tgccaccat 
      ggtgtggaa gaccagcca atgcgccag ataatgaaa 
      cacttccac agctgtacc gacaagcct cgagttctt 
      ggtcttaca gccacgtta ttaaatggc aattgcaaa 
      ttgtcgaaa gtcatggat gaaataagg tctttggag 
      gtgaccttc cacagtaaa gtggccact gtggaagga 
      ttagacgta gttgttgga tattcgacc aatccgcaa 
      gaactattt aaagtttgc cagccgggc gctttgtcg 
      cttgatgcc aaacaagta ctgaacaac ttgcgtcaa 
      ctgataaat gatttggaa catattaat atcaaagat 
      gagcaaaac agtgtgaat ttattacag tctgaaacc 
      ttgaaaccg cttgagcca tccgatgtg ttgaaaagt 
      ctgagaaac ttgatttca gacctaatg attcacatt 
      gaaatgtta ggggcattc ggtggtcac attgcctgt 
      gtggcgcat atgatccaa atagaacgt ataaaaaag 
      cactgccag aaccaccaa ctcttcata gtgttgaat 
      tacgtcatg accataatg gggacgaca aaactactt 
      ctggaggaa accatggcc ggttatgag ccgctcgaa 
      aaaattcga aaattctcc tctgacaaa gtactgaaa 
      gtgtttgaa attttggac gaatacaaa accaaatcg 
      gacgaggaa ctttgctgt ttagtcttc accaaaagg 
      cggttcaca gccaaggtc ctccaccac attatcgac 
      aaagctagc caagttgat cccaaattc tatcatatc 
      aagtcaaat ttcgtcgtt ggaaataag aataatccc 
      tacaacgac acccgagag aatttatat ataacaaaa 
      aagaaccga gaggtgctc aacagcttt gtcagcaag 
      gaaatcaac gttttggtc agctcgaac gtgttggag 
      gagggagtg gacatacct aagtgcact ctcgtcatc 
      aaattcgac aaatcggag gactaccgt tcgtatatc 
      cagtcgaaa ggacgcgca agacatatc aaaagttta 
      tactacacc attgtagaa acgactgac gtagcaaag 
      tacgataaa aaatactcc gctttcaag gaaatcgaa 
      aacctggtg aatgatctt ctcatcggc aaaaattct 
      gaaagagat catccaaac ttgtcggaa atccgcaac 
      atgtataat gaagacaag cttgagccc tactacgta 
      aacggcccc aattcggca caagtaaac atgacctcg 
      gccgtagct ctcttgtgc cgttactgc agtaattta 
      gcaagtgac aaatacacc acttacgcc cctgagtgg 
      tattatgaa gaggattct agcagcgca aaattgcgg 
      gttgtgata tttttgccc gttgtgtgc cctcttatc 
      gaccccatt gtcggccct tacatgcac aacaaaaaa 
      gacgcaaaa agagcagcc gcactagtc gcatgtata 
      aagctacac cagtgcggc gaacttgac aataatttg 
      ttgccatgg aagaagcag ttggatgaa gccgatgtt 
      tcctatctt ttcacgcac tggccacaa gaaaaggaa 
      agcgacgcc ggaaataag aaaaagaag aggttgcac 
      gataaagag atcgctccg agtgtgaag agtgcaatc 
      cagccggac agagtcctc tatttgcac actattaac 
      ataaatccg caatacaaa agaagcgac gatttgaaa 
      aacgcggtc actatttac gacctgtac aaaacaccg 
      ttaaagttt ggtttgtta tcgccgaaa ccgttgccc 
      gatttgtgc aaatttccc ctctttgat tcaaatgga 
      acgttggaa attgaaatt aggaacaat gttagagag 
      gtggagttt gcagctaac gagatgaag gaaatgagg 
      gagttccat tttttggta ttcaacgac cttttggag 
      attctgaaa gagttttta attttcgac aacactggg 
      atgaattcg gagatgttg cttgttgta ccggttcag 
      gatcggtgt ggggatgtt tgcgtcgat tttcgagtg 
      attcgagac aataaaaat ttgaaaaat aagctcgaa 
      ccggcggct acggaacgc ataaatttg aacgtaact 
      gaggaaact tacttacat aagattgtc tctccgtgg 
      tatagatcg ccaccaaag atgtacgtt gtgacgaag 
      gtttgtcct gataagagc gccctgagc cggtttcct 
      aaccacgaa tatcccaat ttcgtgtcg tattactcc 
      gaaaaacac tctttgtcg atattggac ccttcgcaa 
      ccgctgctc ttggttaaa gggctttcc gaacggttg 
      aacgctttc aaaccgaga ggtgccggt ggcaagaga 
      aagaaggaa aagatgtat gaagaattg gaagaatat 
      ctaatacca gagctggtg atcaagcaa gaatttccg 
      tcgtgtttg tggatacaa gcgcgattt ttgccaagc 
      attttatcc cgcttggct taccttcta aaactgcaa 
      caactccaa gttgacata gctcgtggt attggggcc 
      aaagccgaa tatttgaaa gactgtccg ccgctggag 
      ttaaacctc cacttattg cattatgag cccaatgac 
      ccgcaacta acgcaagaa tcagacaag tccactcct 
      ttgattgac aattgcctt gcgttggag tgcccgaaa 
      aatttgcga acgattcaa tacaataaa gattttgcg 
      gcgaaaatg ctggaagcg gagtactac tggaagact 
      attgaagaa cccaaagac atagagcgc aatattaac 
      gtgactgtt atggatata gagtattac gagactttt 
      atttcgcat cagccttcc aaaaccggg cgtttgttg 
      aagaatgac agcccggtt aagcaacag aatgtgccc 
      gctattacg tacgattgc caatttgaa gcgaaacaa 
      ttgcaaatt ctggacgta caattcgac aatcagagc 
      ccgaacttg tgccagatt tatcaagcc ttgacggca 
      gctgaagcc aacgatatc gtcaacctc gaacggctt 
      gaaaccctc ggcgactct ttcttaaaa ttcgtagct 
      tccttgtac attattttc aaatttccg acctacaac 
      gagggcaaa tcgaccact ctcaaaggg aagctggtc 
      agcaacaag aatttgtac tacttggga gtgaggaaa 
      aatctgggc ggtattttg aaaaacagc gacttgtcc 
      ccgagcgat tgggtccct ccgtgcttt tgcatccca 
      caaacaatc agcaaagca atcggcaat aaagagtat 
      tcagtagtc tcgttattt aactgttgc atttcgccg 
      gaggagcaa gtttcaggt aacttgaac cgaaagact 
      ttgagcgat atgacaacg gaagaaatt gccccggat 
      gaggaaaat tcgtacggc aatatgtgc aactttttg 
      aacaaacaa tacgttggt gataagagc atagcggac 
      tctgttgag gccctccta ggcgcctac tttctaagt 
      ggcgggatt caaggcggg atcaaattc atggaatgg 
      atcggcatt ttgcccctt tctgaacaa atccaacgc 
      ctgatcgaa accactcaa gtcgacccc gtcctcaat 
      aaaaaatcc acgaaaact gatgtcgat ttccacatg 
      ccacagtgg cgcgaaatc gagcaacgt ctcggttat 
      acgtttacc aaccgggcg tttttactc caagcttta 
      actcattct tcctattcg ccgaatcgc ataacactg 
      agctacgaa cggttggag tttcttggt gatgctgtg 
      cttgatttc ctcatcacg tgttatatt ttcgaacat 
      tgtgggcat ttggagccg ggtcaagtg accgatttg 
      cggtcatcc cttgtcaac aataacacg ttcgcaagc 
      cttgttgtc cgatgcggg tttcacaag tttttgctc 
      atgatgaat tcgaatttg caaggtcat attgacaag 
      tttgccgat taccttgct tcaaaaaat tacgtgatc 
      gacgatgaa gttctgatt ttgttggag gaggatgag 
      atgaatatc gccgagtac gtcgatgtg cctaaagtt 
      ttgggtgat attttcgaa gcccttgct ggcgcaatt 
      tatctagat agcaataag gatttgaag actgtttgg 
      agggtgttt tacaagatt atctggcgc gaaattgat 
      ctttttagc aagaatgtg ccaaaaaat gtcatcagg 
      agattgtac gagtgccat actgtttac cctccgcaa 
      tttagtaaa gcgttggaa gttggtaat caaaagact 
      atggtttct ttagacttt atgtgtgaa ggtagaaag 
      aaacgagtt catgggttt ggaacgaac aaaatcttg 
      gctaaaagg gccgctgcc aaaattgct cttcgtgct 
      ttaaagttg taa
    mRNA sequence
    • >TC001108 RA
      tgaaatgtc agaaaaaaa ttagttttt tttaaagaa 
      ttgtgttca ctcattcat ctccttatc atcttcatt 
      gtgaacacg tatccaaaa aaatttttt tcgcccccg 
      ctttaagaa ctttttatt aaaatggac gaagaagac 
      gaacttaaa cctcgtaac taccaagtc aatttgatg 
      gaaattgcc attcgggaa aacacaatt atttatcta 
      ccgactgga tcgggtaaa acttttatt gcaataatg 
      gttttgaag cagttgtgc gcccccata ttgagaccc 
      tacagcgat ggtggaaag atttcagtt atattggtt 
      aattctgtg gctttggtg gatcaacat ggtaagtat 
      gtaagagac cacgccacg ttcagtgtg ggaacttac 
      actggagag atgaatgtg gatttttgg agcgaggca 
      gaatgggaa cagcaattt aataagtat caagttgtt 
      ataatgacc agccaaatc atggtaaat ctcatcaac 
      aaccgcttc atagatttg ggaaaagta aacttgatg 
      attttcgat gaatgccac catggtgtg gaagaccag 
      ccaatgcgc cagataatg aaacacttc cacagctgt 
      accgacaag cctcgagtt cttggtctt acagccacg 
      ttattaaat ggcaattgc aaattgtcg aaagtcatg 
      gatgaaata aggtctttg gaggtgacc ttccacagt 
      aaagtggcc actgtggaa ggattagac gtagttgtt 
      ggatattcg accaatccg caagaacta tttaaagtt 
      tgccagccg ggcgctttg tcgcttgat gccaaacaa 
      gtactgaac aacttgcgt caactgata aatgatttg 
      gaacatatt aatatcaaa gatgagcaa aacagtgtg 
      aatttatta cagtctgaa accttgaaa ccgcttgag 
      ccatccgat gtgttgaaa agtctgaga aacttgatt 
      tcagaccta atgattcac attgaaatg ttaggggca 
      ttcggtggt cacattgcc tgtgtggcg catatgatc 
      caaatagaa cgtataaaa aagcactgc cagaaccac 
      caactcttc atagtgttg aattacgtc atgaccata 
      atggggacg acaaaacta cttctggag gaaaccatg 
      gccggttat gagccgctc gaaaaaatt cgaaaattc 
      tcctctgac aaagtactg aaagtgttt gaaattttg 
      gacgaatac aaaaccaaa tcggacgag gaactttgc 
      tgtttagtc ttcaccaaa aggcggttc acagccaag 
      gtcctccac cacattatc gacaaagct agccaagtt 
      gatcccaaa ttctatcat atcaagtca aatttcgtc 
      gttggaaat aagaataat ccctacaac gacacccga 
      gagaattta tatataaca aaaaagaac cgagaggtg 
      ctcaacagc tttgtcagc aaggaaatc aacgttttg 
      gtcagctcg aacgtgttg gaggaggga gtggacata 
      cctaagtgc actctcgtc atcaaattc gacaaatcg 
      gaggactac cgttcgtat atccagtcg aaaggacgc 
      gcaagacat atcaaaagt ttatactac accattgta 
      gaaacgact gacgtagca aagtacgat aaaaaatac 
      tccgctttc aaggaaatc gaaaacctg gtgaatgat 
      cttctcatc ggcaaaaat tctgaaaga gatcatcca 
      aacttgtcg gaaatccgc aacatgtat aatgaagac 
      aagcttgag ccctactac gtaaacggc cccaattcg 
      gcacaagta aacatgacc tcggccgta gctctcttg 
      tgccgttac tgcagtaat ttagcaagt gacaaatac 
      accacttac gcccctgag tggtattat gaagaggat 
      tctagcagc gcaaaattg cgggttgtg atatttttg 
      cccgttgtg tgccctctt atcgacccc attgtcggc 
      ccttacatg cacaacaaa aaagacgca aaaagagca 
      gccgcacta gtcgcatgt ataaagcta caccagtgc 
      ggcgaactt gacaataat ttgttgcca tggaagaag 
      cagttggat gaagccgat gtttcctat cttttcacg 
      cactggcca caagaaaag gaaagcgac gccggaaat 
      aagaaaaag aagaggttg cacgataaa gagatcgct 
      ccgagtgtg aagagtgca atccagccg gacagagtc 
      ctctatttg cacactatt aacataaat ccgcaatac 
      aaaagaagc gacgatttg aaaaacgcg gtcactatt 
      tacgacctg tacaaaaca ccgttaaag tttggtttg 
      ttatcgccg aaaccgttg cccgatttg tgcaaattt 
      cccctcttt gattcaaat ggaacgttg gaaattgaa 
      attaggaac aatgttaga gaggtggag tttgcagct 
      aacgagatg aaggaaatg agggagttc cattttttg 
      gtattcaac gaccttttg gagattctg aaagagttt 
      ttaattttc gacaacact gggatgaat tcggagatg 
      ttgcttgtt gtaccggtt caggatcgg tgtggggat 
      gtttgcgtc gattttcga gtgattcga gacaataaa 
      aatttgaaa aataagctc gaaccggcg gctacggaa 
      cgcataaat ttgaacgta actgaggaa acttactta 
      cataagatt gtctctccg tggtataga tcgccacca 
      aagatgtac gttgtgacg aaggtttgt cctgataag 
      agcgccctg agccggttt cctaaccac gaatatccc 
      aatttcgtg tcgtattac tccgaaaaa cactctttg 
      tcgatattg gacccttcg caaccgctg ctcttggtt 
      aaagggctt tccgaacgg ttgaacgct ttcaaaccg 
      agaggtgcc ggtggcaag agaaagaag gaaaagatg 
      tatgaagaa ttggaagaa tatctaata ccagagctg 
      gtgatcaag caagaattt ccgtcgtgt ttgtggata 
      caagcgcga tttttgcca agcatttta tcccgcttg 
      gcttacctt ctaaaactg caacaactc caagttgac 
      atagctcgt ggtattggg gccaaagcc gaatatttg 
      aaagactgt ccgccgctg gagttaaac ctccactta 
      ttgcattat gagcccaat gacccgcaa ctaacgcaa 
      gaatcagac aagtccact cctttgatt gacaattgc 
      cttgcgttg gagtgcccg aaaaatttg cgaacgatt 
      caatacaat aaagatttt gcggcgaaa atgctggaa 
      gcggagtac tactggaag actattgaa gaacccaaa 
      gacatagag cgcaatatt aacgtgact gttatggat 
      atagagtat tacgagact tttatttcg catcagcct 
      tccaaaacc gggcgtttg ttgaagaat gacagcccg 
      gttaagcaa cagaatgtg cccgctatt acgtacgat 
      tgccaattt gaagcgaaa caattgcaa attctggac 
      gtacaattc gacaatcag agcccgaac ttgtgccag 
      atttatcaa gccttgacg gcagctgaa gccaacgat 
      atcgtcaac ctcgaacgg cttgaaacc ctcggcgac 
      tctttctta aaattcgta gcttccttg tacattatt 
      ttcaaattt ccgacctac aacgagggc aaatcgacc 
      actctcaaa gggaagctg gtcagcaac aagaatttg 
      tactacttg ggagtgagg aaaaatctg ggcggtatt 
      ttgaaaaac agcgacttg tccccgagc gattgggtc 
      cctccgtgc ttttgcatc ccacaaaca atcagcaaa 
      gcaatcggc aataaagag tattcagta gtctcgtta 
      tttaactgt tgcatttcg ccggaggag caagtttca 
      ggtaacttg aaccgaaag actttgagc gatatgaca 
      acggaagaa attgccccg gatgaggaa aattcgtac 
      ggcaatatg tgcaacttt ttgaacaaa caatacgtt 
      ggtgataag agcatagcg gactctgtt gaggccctc 
      ctaggcgcc tactttcta agtggcggg attcaaggc 
      gggatcaaa ttcatggaa tggatcggc attttgccc 
      ctttctgaa caaatccaa cgcctgatc gaaaccact 
      caagtcgac cccgtcctc aataaaaaa tccacgaaa 
      actgatgtc gatttccac atgccacag tggcgcgaa 
      atcgagcaa cgtctcggt tatacgttt accaaccgg 
      gcgttttta ctccaagct ttaactcat tcttcctat 
      tcgccgaat cgcataaca ctgagctac gaacggttg 
      gagtttctt ggtgatgct gtgcttgat ttcctcatc 
      acgtgttat attttcgaa cattgtggg catttggag 
      ccgggtcaa gtgaccgat ttgcggtca tcccttgtc 
      aacaataac acgttcgca agccttgtt gtccgatgc 
      gggtttcac aagtttttg ctcatgatg aattcgaat 
      ttgcaaggt catattgac aagtttgcc gattacctt 
      gcttcaaaa aattacgtg atcgacgat gaagttctg 
      attttgttg gaggaggat gagatgaat atcgccgag 
      tacgtcgat gtgcctaaa gttttgggt gatattttc 
      gaagccctt gctggcgca atttatcta gatagcaat 
      aaggatttg aagactgtt tggagggtg ttttacaag 
      attatctgg cgcgaaatt gatcttttt agcaagaat 
      gtgccaaaa aatgtcatc aggagattg tacgagtgc 
      catactgtt taccctccg caatttagt aaagcgttg 
      gaagttggt aatcaaaag actatggtt tctttagac 
      tttatgtgt gaaggtaga aagaaacga gttcatggg 
      tttggaacg aacaaaatc ttggctaaa agggccgct 
      gccaaaatt gctcttcgt gctttaaag ttgtaatta 
      acgcacgaa gtcacaatg cgcttatat tgattcagt 
      tactcgcta ggattgttt gaaaaatgc tgttttcca 
      ttgttcaac tatataccg tcagttaaa ttcattcgc 
      tttgatacc tatcacaaa aatggtgaa tttaactca 
      cgctgataa ttaagtagg agaattttt aaattacag 
      ccaaccttt aatttgtca ttaggtggt tccaaaaat 
      aattcgttt tcaaattaa aactgtttc aagttaacc 
      actcaaccg cattttaaa actgactgg ttaacataa 
      aactgttta atttaaaaa ctgttgcga actttagtc 
      tatacttta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY