| General information | |||
| TC001108 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 11056172..11062496 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC001108 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_07878 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
mature egg not present - Number of animals showing the phenotype: 2
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC001108 RA
MDEEDELKP RNYQVNLME IAIRENTII YLPTGSGKT FIAIMVLKQ LCAPILRPY SDGGKISVI LVNSVALVD QHGKYVRDH ATFSVGTYT GEMNVDFWS EAEWEQQFN KYQVVIMTS QIMVNLINN RFIDLGKVN LMIFDECHH GVEDQPMRQ IMKHFHSCT DKPRVLGLT ATLLNGNCK LSKVMDEIR SLEVTFHSK VATVEGLDV VVGYSTNPQ ELFKVCQPG ALSLDAKQV LNNLRQLIN DLEHINIKD EQNSVNLLQ SETLKPLEP SDVLKSLRN LISDLMIHI EMLGAFGGH IACVAHMIQ IERIKKHCQ NHQLFIVLN YVMTIMGTT KLLLEETMA GYEPLEKIR KFSSDKVLK VFEILDEYK TKSDEELCC LVFTKRRFT AKVLHHIID KASQVDPKF YHIKSNFVV GNKNNPYND TRENLYITK KNREVLNSF VSKEINVLV SSNVLEEGV DIPKCTLVI KFDKSEDYR SYIQSKGRA RHIKSLYYT IVETTDVAK YDKKYSAFK EIENLVNDL LIGKNSERD HPNLSEIRN MYNEDKLEP YYVNGPNSA QVNMTSAVA LLCRYCSNL ASDKYTTYA PEWYYEEDS SSAKLRVVI FLPVVCPLI DPIVGPYMH NKKDAKRAA ALVACIKLH QCGELDNNL LPWKKQLDE ADVSYLFTH WPQEKESDA GNKKKKRLH DKEIAPSVK SAIQPDRVL YLHTININP QYKRSDDLK NAVTIYDLY KTPLKFGLL SPKPLPDLC KFPLFDSNG TLEIEIRNN VREVEFAAN EMKEMREFH FLVFNDLLE ILKEFLIFD NTGMNSEML LVVPVQDRC GDVCVDFRV IRDNKNLKN KLEPAATER INLNVTEET YLHKIVSPW YRSPPKMYV VTKVCPDKS ALSRFPNHE YPNFVSYYS EKHSLSILD PSQPLLLVK GLSERLNAF KPRGAGGKR KKEKMYEEL EEYLIPELV IKQEFPSCL WIQARFLPS ILSRLAYLL KLQQLQVDI ARGIGAKAE YLKDCPPLE LNLHLLHYE PNDPQLTQE SDKSTPLID NCLALECPK NLRTIQYNK DFAAKMLEA EYYWKTIEE PKDIERNIN VTVMDIEYY ETFISHQPS KTGRLLKND SPVKQQNVP AITYDCQFE AKQLQILDV QFDNQSPNL CQIYQALTA AEANDIVNL ERLETLGDS FLKFVASLY IIFKFPTYN EGKSTTLKG KLVSNKNLY YLGVRKNLG GILKNSDLS PSDWVPPCF CIPQTISKA IGNKEYSVV SLFNCCISP EEQVSGNLN RKTLSDMTT EEIAPDEEN SYGNMCNFL NKQYVGDKS IADSVEALL GAYFLSGGI QGGIKFMEW IGILPLSEQ IQRLIETTQ VDPVLNKKS TKTDVDFHM PQWREIEQR LGYTFTNRA FLLQALTHS SYSPNRITL SYERLEFLG DAVLDFLIT CYIFEHCGH LEPGQVTDL RSSLVNNNT FASLVVRCG FHKFLLMMN SNLQGHIDK FADYLASKN YVIDDEVLI LLEEDEMNI AEYVDVPKV LGDIFEALA GAIYLDSNK DLKTVWRVF YKIIWREID LFSKNVPKN VIRRLYECH TVYPPQFSK ALEVGNQKT MVSLDFMCE GRKKRVHGF GTNKILAKR AAAKIALRA LKL
- >TC001108 RA
atggacgaa gaagacgaa cttaaacct cgtaactac caagtcaat ttgatggaa attgccatt cgggaaaac acaattatt tatctaccg actggatcg ggtaaaact tttattgca ataatggtt ttgaagcag ttgtgcgcc cccatattg agaccctac agcgatggt ggaaagatt tcagttata ttggttaat tctgtggct ttggtggat caacatggt aagtatgta agagaccac gccacgttc agtgtggga acttacact ggagagatg aatgtggat ttttggagc gaggcagaa tgggaacag caatttaat aagtatcaa gttgttata atgaccagc caaatcatg gtaaatctc atcaacaac cgcttcata gatttggga aaagtaaac ttgatgatt ttcgatgaa tgccaccat ggtgtggaa gaccagcca atgcgccag ataatgaaa cacttccac agctgtacc gacaagcct cgagttctt ggtcttaca gccacgtta ttaaatggc aattgcaaa ttgtcgaaa gtcatggat gaaataagg tctttggag gtgaccttc cacagtaaa gtggccact gtggaagga ttagacgta gttgttgga tattcgacc aatccgcaa gaactattt aaagtttgc cagccgggc gctttgtcg cttgatgcc aaacaagta ctgaacaac ttgcgtcaa ctgataaat gatttggaa catattaat atcaaagat gagcaaaac agtgtgaat ttattacag tctgaaacc ttgaaaccg cttgagcca tccgatgtg ttgaaaagt ctgagaaac ttgatttca gacctaatg attcacatt gaaatgtta ggggcattc ggtggtcac attgcctgt gtggcgcat atgatccaa atagaacgt ataaaaaag cactgccag aaccaccaa ctcttcata gtgttgaat tacgtcatg accataatg gggacgaca aaactactt ctggaggaa accatggcc ggttatgag ccgctcgaa aaaattcga aaattctcc tctgacaaa gtactgaaa gtgtttgaa attttggac gaatacaaa accaaatcg gacgaggaa ctttgctgt ttagtcttc accaaaagg cggttcaca gccaaggtc ctccaccac attatcgac aaagctagc caagttgat cccaaattc tatcatatc aagtcaaat ttcgtcgtt ggaaataag aataatccc tacaacgac acccgagag aatttatat ataacaaaa aagaaccga gaggtgctc aacagcttt gtcagcaag gaaatcaac gttttggtc agctcgaac gtgttggag gagggagtg gacatacct aagtgcact ctcgtcatc aaattcgac aaatcggag gactaccgt tcgtatatc cagtcgaaa ggacgcgca agacatatc aaaagttta tactacacc attgtagaa acgactgac gtagcaaag tacgataaa aaatactcc gctttcaag gaaatcgaa aacctggtg aatgatctt ctcatcggc aaaaattct gaaagagat catccaaac ttgtcggaa atccgcaac atgtataat gaagacaag cttgagccc tactacgta aacggcccc aattcggca caagtaaac atgacctcg gccgtagct ctcttgtgc cgttactgc agtaattta gcaagtgac aaatacacc acttacgcc cctgagtgg tattatgaa gaggattct agcagcgca aaattgcgg gttgtgata tttttgccc gttgtgtgc cctcttatc gaccccatt gtcggccct tacatgcac aacaaaaaa gacgcaaaa agagcagcc gcactagtc gcatgtata aagctacac cagtgcggc gaacttgac aataatttg ttgccatgg aagaagcag ttggatgaa gccgatgtt tcctatctt ttcacgcac tggccacaa gaaaaggaa agcgacgcc ggaaataag aaaaagaag aggttgcac gataaagag atcgctccg agtgtgaag agtgcaatc cagccggac agagtcctc tatttgcac actattaac ataaatccg caatacaaa agaagcgac gatttgaaa aacgcggtc actatttac gacctgtac aaaacaccg ttaaagttt ggtttgtta tcgccgaaa ccgttgccc gatttgtgc aaatttccc ctctttgat tcaaatgga acgttggaa attgaaatt aggaacaat gttagagag gtggagttt gcagctaac gagatgaag gaaatgagg gagttccat tttttggta ttcaacgac cttttggag attctgaaa gagttttta attttcgac aacactggg atgaattcg gagatgttg cttgttgta ccggttcag gatcggtgt ggggatgtt tgcgtcgat tttcgagtg attcgagac aataaaaat ttgaaaaat aagctcgaa ccggcggct acggaacgc ataaatttg aacgtaact gaggaaact tacttacat aagattgtc tctccgtgg tatagatcg ccaccaaag atgtacgtt gtgacgaag gtttgtcct gataagagc gccctgagc cggtttcct aaccacgaa tatcccaat ttcgtgtcg tattactcc gaaaaacac tctttgtcg atattggac ccttcgcaa ccgctgctc ttggttaaa gggctttcc gaacggttg aacgctttc aaaccgaga ggtgccggt ggcaagaga aagaaggaa aagatgtat gaagaattg gaagaatat ctaatacca gagctggtg atcaagcaa gaatttccg tcgtgtttg tggatacaa gcgcgattt ttgccaagc attttatcc cgcttggct taccttcta aaactgcaa caactccaa gttgacata gctcgtggt attggggcc aaagccgaa tatttgaaa gactgtccg ccgctggag ttaaacctc cacttattg cattatgag cccaatgac ccgcaacta acgcaagaa tcagacaag tccactcct ttgattgac aattgcctt gcgttggag tgcccgaaa aatttgcga acgattcaa tacaataaa gattttgcg gcgaaaatg ctggaagcg gagtactac tggaagact attgaagaa cccaaagac atagagcgc aatattaac gtgactgtt atggatata gagtattac gagactttt atttcgcat cagccttcc aaaaccggg cgtttgttg aagaatgac agcccggtt aagcaacag aatgtgccc gctattacg tacgattgc caatttgaa gcgaaacaa ttgcaaatt ctggacgta caattcgac aatcagagc ccgaacttg tgccagatt tatcaagcc ttgacggca gctgaagcc aacgatatc gtcaacctc gaacggctt gaaaccctc ggcgactct ttcttaaaa ttcgtagct tccttgtac attattttc aaatttccg acctacaac gagggcaaa tcgaccact ctcaaaggg aagctggtc agcaacaag aatttgtac tacttggga gtgaggaaa aatctgggc ggtattttg aaaaacagc gacttgtcc ccgagcgat tgggtccct ccgtgcttt tgcatccca caaacaatc agcaaagca atcggcaat aaagagtat tcagtagtc tcgttattt aactgttgc atttcgccg gaggagcaa gtttcaggt aacttgaac cgaaagact ttgagcgat atgacaacg gaagaaatt gccccggat gaggaaaat tcgtacggc aatatgtgc aactttttg aacaaacaa tacgttggt gataagagc atagcggac tctgttgag gccctccta ggcgcctac tttctaagt ggcgggatt caaggcggg atcaaattc atggaatgg atcggcatt ttgcccctt tctgaacaa atccaacgc ctgatcgaa accactcaa gtcgacccc gtcctcaat aaaaaatcc acgaaaact gatgtcgat ttccacatg ccacagtgg cgcgaaatc gagcaacgt ctcggttat acgtttacc aaccgggcg tttttactc caagcttta actcattct tcctattcg ccgaatcgc ataacactg agctacgaa cggttggag tttcttggt gatgctgtg cttgatttc ctcatcacg tgttatatt ttcgaacat tgtgggcat ttggagccg ggtcaagtg accgatttg cggtcatcc cttgtcaac aataacacg ttcgcaagc cttgttgtc cgatgcggg tttcacaag tttttgctc atgatgaat tcgaatttg caaggtcat attgacaag tttgccgat taccttgct tcaaaaaat tacgtgatc gacgatgaa gttctgatt ttgttggag gaggatgag atgaatatc gccgagtac gtcgatgtg cctaaagtt ttgggtgat attttcgaa gcccttgct ggcgcaatt tatctagat agcaataag gatttgaag actgtttgg agggtgttt tacaagatt atctggcgc gaaattgat ctttttagc aagaatgtg ccaaaaaat gtcatcagg agattgtac gagtgccat actgtttac cctccgcaa tttagtaaa gcgttggaa gttggtaat caaaagact atggtttct ttagacttt atgtgtgaa ggtagaaag aaacgagtt catgggttt ggaacgaac aaaatcttg gctaaaagg gccgctgcc aaaattgct cttcgtgct ttaaagttg taa
- >TC001108 RA
tgaaatgtc agaaaaaaa ttagttttt tttaaagaa ttgtgttca ctcattcat ctccttatc atcttcatt gtgaacacg tatccaaaa aaatttttt tcgcccccg ctttaagaa ctttttatt aaaatggac gaagaagac gaacttaaa cctcgtaac taccaagtc aatttgatg gaaattgcc attcgggaa aacacaatt atttatcta ccgactgga tcgggtaaa acttttatt gcaataatg gttttgaag cagttgtgc gcccccata ttgagaccc tacagcgat ggtggaaag atttcagtt atattggtt aattctgtg gctttggtg gatcaacat ggtaagtat gtaagagac cacgccacg ttcagtgtg ggaacttac actggagag atgaatgtg gatttttgg agcgaggca gaatgggaa cagcaattt aataagtat caagttgtt ataatgacc agccaaatc atggtaaat ctcatcaac aaccgcttc atagatttg ggaaaagta aacttgatg attttcgat gaatgccac catggtgtg gaagaccag ccaatgcgc cagataatg aaacacttc cacagctgt accgacaag cctcgagtt cttggtctt acagccacg ttattaaat ggcaattgc aaattgtcg aaagtcatg gatgaaata aggtctttg gaggtgacc ttccacagt aaagtggcc actgtggaa ggattagac gtagttgtt ggatattcg accaatccg caagaacta tttaaagtt tgccagccg ggcgctttg tcgcttgat gccaaacaa gtactgaac aacttgcgt caactgata aatgatttg gaacatatt aatatcaaa gatgagcaa aacagtgtg aatttatta cagtctgaa accttgaaa ccgcttgag ccatccgat gtgttgaaa agtctgaga aacttgatt tcagaccta atgattcac attgaaatg ttaggggca ttcggtggt cacattgcc tgtgtggcg catatgatc caaatagaa cgtataaaa aagcactgc cagaaccac caactcttc atagtgttg aattacgtc atgaccata atggggacg acaaaacta cttctggag gaaaccatg gccggttat gagccgctc gaaaaaatt cgaaaattc tcctctgac aaagtactg aaagtgttt gaaattttg gacgaatac aaaaccaaa tcggacgag gaactttgc tgtttagtc ttcaccaaa aggcggttc acagccaag gtcctccac cacattatc gacaaagct agccaagtt gatcccaaa ttctatcat atcaagtca aatttcgtc gttggaaat aagaataat ccctacaac gacacccga gagaattta tatataaca aaaaagaac cgagaggtg ctcaacagc tttgtcagc aaggaaatc aacgttttg gtcagctcg aacgtgttg gaggaggga gtggacata cctaagtgc actctcgtc atcaaattc gacaaatcg gaggactac cgttcgtat atccagtcg aaaggacgc gcaagacat atcaaaagt ttatactac accattgta gaaacgact gacgtagca aagtacgat aaaaaatac tccgctttc aaggaaatc gaaaacctg gtgaatgat cttctcatc ggcaaaaat tctgaaaga gatcatcca aacttgtcg gaaatccgc aacatgtat aatgaagac aagcttgag ccctactac gtaaacggc cccaattcg gcacaagta aacatgacc tcggccgta gctctcttg tgccgttac tgcagtaat ttagcaagt gacaaatac accacttac gcccctgag tggtattat gaagaggat tctagcagc gcaaaattg cgggttgtg atatttttg cccgttgtg tgccctctt atcgacccc attgtcggc ccttacatg cacaacaaa aaagacgca aaaagagca gccgcacta gtcgcatgt ataaagcta caccagtgc ggcgaactt gacaataat ttgttgcca tggaagaag cagttggat gaagccgat gtttcctat cttttcacg cactggcca caagaaaag gaaagcgac gccggaaat aagaaaaag aagaggttg cacgataaa gagatcgct ccgagtgtg aagagtgca atccagccg gacagagtc ctctatttg cacactatt aacataaat ccgcaatac aaaagaagc gacgatttg aaaaacgcg gtcactatt tacgacctg tacaaaaca ccgttaaag tttggtttg ttatcgccg aaaccgttg cccgatttg tgcaaattt cccctcttt gattcaaat ggaacgttg gaaattgaa attaggaac aatgttaga gaggtggag tttgcagct aacgagatg aaggaaatg agggagttc cattttttg gtattcaac gaccttttg gagattctg aaagagttt ttaattttc gacaacact gggatgaat tcggagatg ttgcttgtt gtaccggtt caggatcgg tgtggggat gtttgcgtc gattttcga gtgattcga gacaataaa aatttgaaa aataagctc gaaccggcg gctacggaa cgcataaat ttgaacgta actgaggaa acttactta cataagatt gtctctccg tggtataga tcgccacca aagatgtac gttgtgacg aaggtttgt cctgataag agcgccctg agccggttt cctaaccac gaatatccc aatttcgtg tcgtattac tccgaaaaa cactctttg tcgatattg gacccttcg caaccgctg ctcttggtt aaagggctt tccgaacgg ttgaacgct ttcaaaccg agaggtgcc ggtggcaag agaaagaag gaaaagatg tatgaagaa ttggaagaa tatctaata ccagagctg gtgatcaag caagaattt ccgtcgtgt ttgtggata caagcgcga tttttgcca agcatttta tcccgcttg gcttacctt ctaaaactg caacaactc caagttgac atagctcgt ggtattggg gccaaagcc gaatatttg aaagactgt ccgccgctg gagttaaac ctccactta ttgcattat gagcccaat gacccgcaa ctaacgcaa gaatcagac aagtccact cctttgatt gacaattgc cttgcgttg gagtgcccg aaaaatttg cgaacgatt caatacaat aaagatttt gcggcgaaa atgctggaa gcggagtac tactggaag actattgaa gaacccaaa gacatagag cgcaatatt aacgtgact gttatggat atagagtat tacgagact tttatttcg catcagcct tccaaaacc gggcgtttg ttgaagaat gacagcccg gttaagcaa cagaatgtg cccgctatt acgtacgat tgccaattt gaagcgaaa caattgcaa attctggac gtacaattc gacaatcag agcccgaac ttgtgccag atttatcaa gccttgacg gcagctgaa gccaacgat atcgtcaac ctcgaacgg cttgaaacc ctcggcgac tctttctta aaattcgta gcttccttg tacattatt ttcaaattt ccgacctac aacgagggc aaatcgacc actctcaaa gggaagctg gtcagcaac aagaatttg tactacttg ggagtgagg aaaaatctg ggcggtatt ttgaaaaac agcgacttg tccccgagc gattgggtc cctccgtgc ttttgcatc ccacaaaca atcagcaaa gcaatcggc aataaagag tattcagta gtctcgtta tttaactgt tgcatttcg ccggaggag caagtttca ggtaacttg aaccgaaag actttgagc gatatgaca acggaagaa attgccccg gatgaggaa aattcgtac ggcaatatg tgcaacttt ttgaacaaa caatacgtt ggtgataag agcatagcg gactctgtt gaggccctc ctaggcgcc tactttcta agtggcggg attcaaggc gggatcaaa ttcatggaa tggatcggc attttgccc ctttctgaa caaatccaa cgcctgatc gaaaccact caagtcgac cccgtcctc aataaaaaa tccacgaaa actgatgtc gatttccac atgccacag tggcgcgaa atcgagcaa cgtctcggt tatacgttt accaaccgg gcgttttta ctccaagct ttaactcat tcttcctat tcgccgaat cgcataaca ctgagctac gaacggttg gagtttctt ggtgatgct gtgcttgat ttcctcatc acgtgttat attttcgaa cattgtggg catttggag ccgggtcaa gtgaccgat ttgcggtca tcccttgtc aacaataac acgttcgca agccttgtt gtccgatgc gggtttcac aagtttttg ctcatgatg aattcgaat ttgcaaggt catattgac aagtttgcc gattacctt gcttcaaaa aattacgtg atcgacgat gaagttctg attttgttg gaggaggat gagatgaat atcgccgag tacgtcgat gtgcctaaa gttttgggt gatattttc gaagccctt gctggcgca atttatcta gatagcaat aaggatttg aagactgtt tggagggtg ttttacaag attatctgg cgcgaaatt gatcttttt agcaagaat gtgccaaaa aatgtcatc aggagattg tacgagtgc catactgtt taccctccg caatttagt aaagcgttg gaagttggt aatcaaaag actatggtt tctttagac tttatgtgt gaaggtaga aagaaacga gttcatggg tttggaacg aacaaaatc ttggctaaa agggccgct gccaaaatt gctcttcgt gctttaaag ttgtaatta acgcacgaa gtcacaatg cgcttatat tgattcagt tactcgcta ggattgttt gaaaaatgc tgttttcca ttgttcaac tatataccg tcagttaaa ttcattcgc tttgatacc tatcacaaa aatggtgaa tttaactca cgctgataa ttaagtagg agaattttt aaattacag ccaaccttt aatttgtca ttaggtggt tccaaaaat aattcgttt tcaaattaa aactgtttc aagttaacc actcaaccg cattttaaa actgactgg ttaacataa aactgttta atttaaaaa ctgttgcga actttagtc tatacttta
