Status
Please wait ...
Gene information
General information
TC032153 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 10273915..10276151
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC032153
    iBeetle screen iB_07873

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032153 RA
      MDDSKLTVR ESSRIIKEK SLDYVPDPR GLGYEFYQA 
MFLNQNAVA QIDAESGCA DSYGNLLER CIRTAIRLL 
VAGITKRDI ICHCSSNHI NSCVPIICA FFLGVPVAP 
VDPTLSYDE AFEILAQIR PRVIFVTPD VVDTIEMAL 
GEINIECEI VLFGSSPDF VEFDSFLEP DEEESHFQP 
LEISDIRET AVIAFTKGQ KDDYKGVCL SHYAIMMQC 
LNTISCCNF GNTFCLSNR EPDKNVNGF ETALSFNTF 
ARIAEIELL CCCTFTGIT KIINQKFDP TFAWRTIDK 
HEVSFMTCT SFQAIQLYK IGKPEGVVG TTLKMLLTQ 
GTSLSKASL YELRKLLPY CFITNCYGL TETTGRVTM 
FAFTKPGDI RDQYENPTS CGKLVSGMT CKIVDPITE 
KILGPNQAG ELRFKSDLM MNGYLKQDH SDLFDKDGW 
VKTGDVALY DDKHRFFVL NRLKDVFKC YSWHIAPVI 
IENVIKEHP AVKDAVVIG IFEDIKAWN QIPMAVVTL 
NRGMRNRVT GKDIQMWAN SKLSKKQQL KAGVKIVKQ 
IPITIAGEP KRQQVKDMV LCENL
    CDS sequence
    • >TC032153 RA
      atggacgac tccaaattg acagtcaga gaaagttcg 
cggattatt aaagagaaa agccttgat tatgttcct 
gatcctcga ggattagga tacgagttt taccaagcg 
atgtttctt aatcaaaat gcagtggct cagattgac 
gcagaaagt ggatgtgcg gattcatat ggcaatctg 
ctcgagaga tgtatccgt actgcgata cgtctactt 
gtcgcaggg attacgaaa cgcgatatc atatgccac 
tgtagcagt aatcacatt aacagttgt gtcccgatt 
atttgcgcc tttttcctg ggagtgccg gtggctccc 
gtagatccc acactctcc tacgacgaa gctttcgaa 
atccttgcc caaatcagg ccgagagtt attttcgtg 
accccggac gtggttgat acaattgaa atggcgctt 
ggtgaaatt aatattgaa tgcgagatt gtgctgttt 
ggctcaagc ccggatttt gttgaattt gactcgttt 
ctggagcca gacgaagaa gaaagccac tttcagccg 
ttggagata tcggatatt agggaaacc gcggtcatt 
gcattcacc aaaggacaa aaagatgat tacaaaggc 
gtctgtttg agccactat gcaatcatg atgcagtgt 
ttaaacaca atatcctgt tgcaatttc ggaaatacg 
ttttgtttg tccaatcgc gagccggac aaaaacgta 
aatgggttc gagacagct ttaagcttc aacacattt 
gccagaatt gctgaaatt gagctcctc tgttgctgc 
actttcacg gggattact aaaataata aaccaaaaa 
tttgatccc acatttgcg tggcgcacc atcgacaaa 
catgaagtg agtttcatg acttgtacg tcgtttcaa 
gcaatccag ttgtacaaa attggaaag cctgaaggg 
gtcgttggc acaactttg aaaatgctg ctaacacaa 
gggacttct ttgtcaaaa gcaagtctg tacgaattg 
cgaaaactt ctcccatat tgctttatt acaaattgt 
tatggatta actgaaacc actggccgt gttacaatg 
tttgcgttt acaaaaccg ggggatata agagatcaa 
tacgaaaat cccacgtct tgtgggaag ctagtgtcg 
ggaatgact tgcaaaatc gtagacccc attacagaa 
aaaattttg ggtccaaat caagccggt gaactgcgc 
tttaaaagc gatttaatg atgaacggt tatctcaaa 
caggaccat tctgatctt ttcgataaa gacggttgg 
gtgaaaact ggagatgtt gcgctttat gacgacaaa 
catcgcttc tttgtgctg aatcgtctg aaagacgtg 
tttaagtgt tacagttgg cacatcgcc cctgtgatt 
attgaaaac gttattaag gaacatccc gctgttaaa 
gacgctgtt gttatcgga atattcgaa gatattaaa 
gcttggaac caaatacct atggcagtg gtgacgctg 
aatagaggc atgcggaac agggtgacc ggcaaggac 
atacaaatg tgggcaaac agcaaatta agcaaaaaa 
caacaacta aaagcgggg gtgaaaatt gtcaaacaa 
attccaatc acaatagcc ggagagccg aaaaggcaa 
caagttaaa gatatggtg ttgtgtgaa aacttgtaa
    mRNA sequence
    • >TC032153 RA
      tgacaatgt cacgcctga gagcctcgt ggcgattat 
tttaaattt tcagtttcg tgaacgcgt ttccaaaat 
tgaaaagtg gggatggac gactccaaa ttgacagtc 
agagaaagt tcgcggatt attaaagag aaaagcctt 
gattatgtt cctgatcct cgaggatta ggatacgag 
ttttaccaa gcgatgttt cttaatcaa aatgcagtg 
gctcagatt gacgcagaa agtggatgt gcggattca 
tatggcaat ctgctcgag agatgtatc cgtactgcg 
atacgtcta cttgtcgca gggattacg aaacgcgat 
atcatatgc cactgtagc agtaatcac attaacagt 
tgtgtcccg attatttgc gcctttttc ctgggagtg 
ccggtggct cccgtagat cccacactc tcctacgac 
gaagctttc gaaatcctt gcccaaatc aggccgaga 
gttattttc gtgaccccg gacgtggtt gatacaatt 
gaaatggcg cttggtgaa attaatatt gaatgcgag 
attgtgctg tttggctca agcccggat tttgttgaa 
tttgactcg tttctggag ccagacgaa gaagaaagc 
cactttcag ccgttggag atatcggat attagggaa 
accgcggtc attgcattc accaaagga caaaaagat 
gattacaaa ggcgtctgt ttgagccac tatgcaatc 
atgatgcag tgtttaaac acaatatcc tgttgcaat 
ttcggaaat acgttttgt ttgtccaat cgcgagccg 
gacaaaaac gtaaatggg ttcgagaca gctttaagc 
ttcaacaca tttgccaga attgctgaa attgagctc 
ctctgttgc tgcactttc acggggatt actaaaata 
ataaaccaa aaatttgat cccacattt gcgtggcgc 
accatcgac aaacatgaa gtgagtttc atgacttgt 
acgtcgttt caagcaatc cagttgtac aaaattgga 
aagcctgaa ggggtcgtt ggcacaact ttgaaaatg 
ctgctaaca caagggact tctttgtca aaagcaagt 
ctgtacgaa ttgcgaaaa cttctccca tattgcttt 
attacaaat tgttatgga ttaactgaa accactggc 
cgtgttaca atgtttgcg tttacaaaa ccgggggat 
ataagagat caatacgaa aatcccacg tcttgtggg 
aagctagtg tcgggaatg acttgcaaa atcgtagac 
cccattaca gaaaaaatt ttgggtcca aatcaagcc 
ggtgaactg cgctttaaa agcgattta atgatgaac 
ggttatctc aaacaggac cattctgat cttttcgat 
aaagacggt tgggtgaaa actggagat gttgcgctt 
tatgacgac aaacatcgc ttctttgtg ctgaatcgt 
ctgaaagac gtgtttaag tgttacagt tggcacatc 
gcccctgtg attattgaa aacgttatt aaggaacat 
cccgctgtt aaagacgct gttgttatc ggaatattc 
gaagatatt aaagcttgg aaccaaata cctatggca 
gtggtgacg ctgaataga ggcatgcgg aacagggtg 
accggcaag gacatacaa atgtgggca aacagcaaa 
ttaagcaaa aaacaacaa ctaaaagcg ggggtgaaa 
attgtcaaa caaattcca atcacaata gccggagag 
ccgaaaagg caacaagtt aaagatatg gtgttgtgt 
gaaaacttg taattgttt ttgtaatat agtacaata 
tttgcgtct gtagttaat tttggtggc tcaaaatct 
cgcaattaa tttcaagcg taaagggta aaacataat 
taatcgttt agtgtgacg cactaatct gcaatgtcc 
aactccgcc cacttctaa ctgtttgtc cattgcggc 
aaacgttat caagataat ctccaagtg ggctacact 
ttgatcaaa attggcttt aaaatcttg gtcttttca 
gtctactaa atttgatta caaaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY