Status
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Gene information
General information
TC032153 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 10273915..10276151
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032153
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_07873

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032153 RA
      MDDSKLTVR ESSRIIKEK SLDYVPDPR GLGYEFYQA 
      MFLNQNAVA QIDAESGCA DSYGNLLER CIRTAIRLL 
      VAGITKRDI ICHCSSNHI NSCVPIICA FFLGVPVAP 
      VDPTLSYDE AFEILAQIR PRVIFVTPD VVDTIEMAL 
      GEINIECEI VLFGSSPDF VEFDSFLEP DEEESHFQP 
      LEISDIRET AVIAFTKGQ KDDYKGVCL SHYAIMMQC 
      LNTISCCNF GNTFCLSNR EPDKNVNGF ETALSFNTF 
      ARIAEIELL CCCTFTGIT KIINQKFDP TFAWRTIDK 
      HEVSFMTCT SFQAIQLYK IGKPEGVVG TTLKMLLTQ 
      GTSLSKASL YELRKLLPY CFITNCYGL TETTGRVTM 
      FAFTKPGDI RDQYENPTS CGKLVSGMT CKIVDPITE 
      KILGPNQAG ELRFKSDLM MNGYLKQDH SDLFDKDGW 
      VKTGDVALY DDKHRFFVL NRLKDVFKC YSWHIAPVI 
      IENVIKEHP AVKDAVVIG IFEDIKAWN QIPMAVVTL 
      NRGMRNRVT GKDIQMWAN SKLSKKQQL KAGVKIVKQ 
      IPITIAGEP KRQQVKDMV LCENL
    CDS sequence
    • >TC032153 RA
      atggacgac tccaaattg acagtcaga gaaagttcg 
      cggattatt aaagagaaa agccttgat tatgttcct 
      gatcctcga ggattagga tacgagttt taccaagcg 
      atgtttctt aatcaaaat gcagtggct cagattgac 
      gcagaaagt ggatgtgcg gattcatat ggcaatctg 
      ctcgagaga tgtatccgt actgcgata cgtctactt 
      gtcgcaggg attacgaaa cgcgatatc atatgccac 
      tgtagcagt aatcacatt aacagttgt gtcccgatt 
      atttgcgcc tttttcctg ggagtgccg gtggctccc 
      gtagatccc acactctcc tacgacgaa gctttcgaa 
      atccttgcc caaatcagg ccgagagtt attttcgtg 
      accccggac gtggttgat acaattgaa atggcgctt 
      ggtgaaatt aatattgaa tgcgagatt gtgctgttt 
      ggctcaagc ccggatttt gttgaattt gactcgttt 
      ctggagcca gacgaagaa gaaagccac tttcagccg 
      ttggagata tcggatatt agggaaacc gcggtcatt 
      gcattcacc aaaggacaa aaagatgat tacaaaggc 
      gtctgtttg agccactat gcaatcatg atgcagtgt 
      ttaaacaca atatcctgt tgcaatttc ggaaatacg 
      ttttgtttg tccaatcgc gagccggac aaaaacgta 
      aatgggttc gagacagct ttaagcttc aacacattt 
      gccagaatt gctgaaatt gagctcctc tgttgctgc 
      actttcacg gggattact aaaataata aaccaaaaa 
      tttgatccc acatttgcg tggcgcacc atcgacaaa 
      catgaagtg agtttcatg acttgtacg tcgtttcaa 
      gcaatccag ttgtacaaa attggaaag cctgaaggg 
      gtcgttggc acaactttg aaaatgctg ctaacacaa 
      gggacttct ttgtcaaaa gcaagtctg tacgaattg 
      cgaaaactt ctcccatat tgctttatt acaaattgt 
      tatggatta actgaaacc actggccgt gttacaatg 
      tttgcgttt acaaaaccg ggggatata agagatcaa 
      tacgaaaat cccacgtct tgtgggaag ctagtgtcg 
      ggaatgact tgcaaaatc gtagacccc attacagaa 
      aaaattttg ggtccaaat caagccggt gaactgcgc 
      tttaaaagc gatttaatg atgaacggt tatctcaaa 
      caggaccat tctgatctt ttcgataaa gacggttgg 
      gtgaaaact ggagatgtt gcgctttat gacgacaaa 
      catcgcttc tttgtgctg aatcgtctg aaagacgtg 
      tttaagtgt tacagttgg cacatcgcc cctgtgatt 
      attgaaaac gttattaag gaacatccc gctgttaaa 
      gacgctgtt gttatcgga atattcgaa gatattaaa 
      gcttggaac caaatacct atggcagtg gtgacgctg 
      aatagaggc atgcggaac agggtgacc ggcaaggac 
      atacaaatg tgggcaaac agcaaatta agcaaaaaa 
      caacaacta aaagcgggg gtgaaaatt gtcaaacaa 
      attccaatc acaatagcc ggagagccg aaaaggcaa 
      caagttaaa gatatggtg ttgtgtgaa aacttgtaa
    mRNA sequence
    • >TC032153 RA
      tgacaatgt cacgcctga gagcctcgt ggcgattat 
      tttaaattt tcagtttcg tgaacgcgt ttccaaaat 
      tgaaaagtg gggatggac gactccaaa ttgacagtc 
      agagaaagt tcgcggatt attaaagag aaaagcctt 
      gattatgtt cctgatcct cgaggatta ggatacgag 
      ttttaccaa gcgatgttt cttaatcaa aatgcagtg 
      gctcagatt gacgcagaa agtggatgt gcggattca 
      tatggcaat ctgctcgag agatgtatc cgtactgcg 
      atacgtcta cttgtcgca gggattacg aaacgcgat 
      atcatatgc cactgtagc agtaatcac attaacagt 
      tgtgtcccg attatttgc gcctttttc ctgggagtg 
      ccggtggct cccgtagat cccacactc tcctacgac 
      gaagctttc gaaatcctt gcccaaatc aggccgaga 
      gttattttc gtgaccccg gacgtggtt gatacaatt 
      gaaatggcg cttggtgaa attaatatt gaatgcgag 
      attgtgctg tttggctca agcccggat tttgttgaa 
      tttgactcg tttctggag ccagacgaa gaagaaagc 
      cactttcag ccgttggag atatcggat attagggaa 
      accgcggtc attgcattc accaaagga caaaaagat 
      gattacaaa ggcgtctgt ttgagccac tatgcaatc 
      atgatgcag tgtttaaac acaatatcc tgttgcaat 
      ttcggaaat acgttttgt ttgtccaat cgcgagccg 
      gacaaaaac gtaaatggg ttcgagaca gctttaagc 
      ttcaacaca tttgccaga attgctgaa attgagctc 
      ctctgttgc tgcactttc acggggatt actaaaata 
      ataaaccaa aaatttgat cccacattt gcgtggcgc 
      accatcgac aaacatgaa gtgagtttc atgacttgt 
      acgtcgttt caagcaatc cagttgtac aaaattgga 
      aagcctgaa ggggtcgtt ggcacaact ttgaaaatg 
      ctgctaaca caagggact tctttgtca aaagcaagt 
      ctgtacgaa ttgcgaaaa cttctccca tattgcttt 
      attacaaat tgttatgga ttaactgaa accactggc 
      cgtgttaca atgtttgcg tttacaaaa ccgggggat 
      ataagagat caatacgaa aatcccacg tcttgtggg 
      aagctagtg tcgggaatg acttgcaaa atcgtagac 
      cccattaca gaaaaaatt ttgggtcca aatcaagcc 
      ggtgaactg cgctttaaa agcgattta atgatgaac 
      ggttatctc aaacaggac cattctgat cttttcgat 
      aaagacggt tgggtgaaa actggagat gttgcgctt 
      tatgacgac aaacatcgc ttctttgtg ctgaatcgt 
      ctgaaagac gtgtttaag tgttacagt tggcacatc 
      gcccctgtg attattgaa aacgttatt aaggaacat 
      cccgctgtt aaagacgct gttgttatc ggaatattc 
      gaagatatt aaagcttgg aaccaaata cctatggca 
      gtggtgacg ctgaataga ggcatgcgg aacagggtg 
      accggcaag gacatacaa atgtgggca aacagcaaa 
      ttaagcaaa aaacaacaa ctaaaagcg ggggtgaaa 
      attgtcaaa caaattcca atcacaata gccggagag 
      ccgaaaagg caacaagtt aaagatatg gtgttgtgt 
      gaaaacttg taattgttt ttgtaatat agtacaata 
      tttgcgtct gtagttaat tttggtggc tcaaaatct 
      cgcaattaa tttcaagcg taaagggta aaacataat 
      taatcgttt agtgtgacg cactaatct gcaatgtcc 
      aactccgcc cacttctaa ctgtttgtc cattgcggc 
      aaacgttat caagataat ctccaagtg ggctacact 
      ttgatcaaa attggcttt aaaatcttg gtcttttca 
      gtctactaa atttgatta caaaat
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