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Gene information
General information
TC000525 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 10211972..10213759
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000525
    iBeetle screen iB_07872

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000525 RA
      MTILMDFDE IAPKNKSAS VDNLLNCDD VRISLASMS 
      SKLSEDSSF SHRIQSFAT NCSESDSLL PSLLTIEAL 
      RHYRTHHYK NFSQSQVEE HVYEPGLLA SSERVVRML 
      AKILRIGVG PDRSSTCYQ PTIFTHSSK MPFFFELFS 
      RTMWLLSKT RREMKASTL SDYEKVIHV LAKQVRVVL 
      EAKPMDFKK MTEKMVETS YNTVVEIWQ KEKEEELQN 
      VLDTNECIQ GLKKTFNKE NETNIYLQR INFIKNGTV 
      FPRFVDKKI NGNFYAKLS NNCRDLLIG DYNALTRTS 
      GRAFGYSLS DVTHLVEGK ACPHVANHS VKDPSLAFS 
      LIINNEFIN FKAADRKSA SYWTDALNL LLGKPNRSS 
      YYTNEMNEL TKMDLSPAI RSN
    CDS sequence
    • >TC000525 RA
      atgactatt ttgatggac tttgatgaa attgctccg 
      aaaaataaa agtgcgtcc gtggacaat ttactcaat 
      tgtgacgat gttagaatt tcactggct tccatgtcg 
      tctaaattg agtgaagat tcaagtttc tcgcatcga 
      attcaaagc tttgccaca aattgtagc gaaagtgac 
      agtctgttg ccgagtctt ttaaccatt gaggctctt 
      agacattac aggacccat cattacaaa aacttcagc 
      cagagccaa gtcgaagag cacgtatat gaacctgga 
      cttctggca tcaagcgag agagttgtg agaatgttg 
      gcaaaaatc ctccgcata ggcgtgggg ccggacaga 
      agcagtacc tgttaccag cctaccatt ttcacgcat 
      tcgtcaaaa atgccgttc ttttttgag cttttctcg 
      cgaacaatg tggctgctt tcaaagaca aggcgcgaa 
      atgaaagct tccacactc tccgactat gaaaaagtc 
      attcatgtg ttagccaaa caagtcaga gtcgtcctt 
      gaggcgaaa ccgatggat ttcaaaaaa atgaccgaa 
      aaaatggtt gaaacttcc tataacact gtagtcgaa 
      atatggcag aaagaaaaa gaggaggaa ttgcaaaac 
      gtgctggat actaatgag tgcattcaa ggcttgaag 
      aaaaccttt aataaagaa aatgaaaca aatatttac 
      ttgcagcga attaatttt ataaaaaat ggtacagtt 
      tttccaaga tttgtcgat aagaaaata aatgggaat 
      ttttacgcc aagctctct aacaactgc cgggattta 
      cttatcggt gattataac gcattaaca agaacttca 
      gggagagct tttgggtac tctctgagc gatgttacg 
      catttagtt gaagggaag gcctgtcca catgttgcc 
      aatcacagt gttaaagat ccttccctt gccttctcc 
      ttaataatt aacaacgaa tttataaat ttcaaagca 
      gcggaccga aaatcggcc agttattgg acagatgca 
      cttaacttg ctactggga aaacccaac cggagttcg 
      tattacacc aacgaaatg aacgaacta acaaaaatg 
      gacttgtcc cccgcaatc cgttcgaac tga
    mRNA sequence
    • >TC000525 RA
      tgcagatgc tctcaccac tcccatagc ggactctga 
      ttacttaag cgagttcga gccgctgga cagcgaagt 
      taaaaaaat gactatttt gatggactt tgatgaaat 
      tgctccgaa aaataaaag tgcgtccgt ggacaattt 
      actcaattg tgacgatgt tagaatttc actggcttc 
      catgtcgtc taaattgag tgaagattc aagtttctc 
      gcatcgaat tcaaagctt tgccacaaa ttgtagcga 
      aagtgacag tctgttgcc gagtctttt aaccattga 
      ggctcttag acattacag gacccatca ttacaaaaa 
      cttcagcca gagccaagt cgaagagca cgtatatga 
      acctggact tctggcatc aagcgagag agttgtgag 
      aatgttggc aaaaatcct ccgcatagg cgtggggcc 
      ggacagaag cagtacctg ttaccagcc taccatttt 
      cacgcattc gtcaaaaat gccgttctt ttttgagct 
      tttctcgcg aacaatgtg gctgctttc aaagacaag 
      gcgcgaaat gaaagcttc cacactctc cgactatga 
      aaaagtcat tcatgtgtt agccaaaca agtcagagt 
      cgtccttga ggcgaaacc gatggattt caaaaaaat 
      gaccgaaaa aatggttga aacttccta taacactgt 
      agtcgaaat atggcagaa agaaaaaga ggaggaatt 
      gcaaaacgt gctggatac taatgagtg cattcaagg 
      cttgaagaa aacctttaa taaagaaaa tgaaacaaa 
      tatttactt gcagcgaat taattttat aaaaaatgg 
      tacagtttt tccaagatt tgtcgataa gaaaataaa 
      tgggaattt ttacgccaa gctctctaa caactgccg 
      ggatttact tatcggtga ttataacgc attaacaag 
      aacttcagg gagagcttt tgggtactc tctgagcga 
      tgttacgca tttagttga agggaaggc ctgtccaca 
      tgttgccaa tcacagtgt taaagatcc ttcccttgc 
      cttctcctt aataattaa caacgaatt tataaattt 
      caaagcagc ggaccgaaa atcggccag ttattggac 
      agatgcact taacttgct actgggaaa acccaaccg 
      gagttcgta ttacaccaa cgaaatgaa cgaactaac 
      aaaaatgga cttgtcccc cgcaatccg ttcgaactg 
      aggccccca ataccccca aagccccaa atgtacccc 
      gaaaaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY