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Gene information
General information
TC001040 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 10114100..10115194
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001040
    iBeetle screen iB_07871

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Adult morphology
    abdominal odoriferous glands size decreased - Number of animals showing the phenotype: 3 (13 dpi)
    Days post injection



    Fertility
    mature egg not present - Number of animals showing the phenotype: 1
    previtellogenic egg chamber amount increased - Number of animals showing the phenotype: 4

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001040 RA
      MSVVTKTAL DTDVSILNH IKYEHFVAG ISGGVTSTL 
      ILHPLDVIK IRFAVHDGR LQTTPRYSG IWNAFTTIF 
      RQEGPRGLY RGVVPNVWG AGSSWGLYF LFYTTIKTK 
      IQKGNANTA LSPGQHLLA ASEAGVMTL FLTNPLWVV 
      KTRLCLQYG GSSQQYKGM VDALVKIYR ADGVRGYYK 
      GLVPGIFGV SHGAVQFMV YEQLKNEYT KHYNVPIST 
      KLDTVQYLS FAALSKFIA AGVTYPYQV VRARLQNQH 
      YSYKGSFDC ITQTWKYEG WRGFYKGLG TNLLRVTPA 
      TMITFVTYE NVSHFLMK
    CDS sequence
    • >TC001040 RA
      atgagcgtg gtgaccaaa acggccctc gacaccgac 
      gtcagcatc ctcaaccac atcaaatat gagcacttc 
      gtcgcgggg ataagtggc ggcgtcacc tcaaccctg 
      attctgcac ccccttgac gttatcaag attcggttc 
      gccgtccac gatggcagg ctccaaacc acgccaagg 
      tactcgggg atttggaac gcattcacg acgattttt 
      cgccaagag gggccgcgc gggctctac cgaggggtt 
      gtccccaac gtttggggg gctggcagc tcatggggg 
      ctctatttc ctgttttac acgactatc aagaccaaa 
      atacagaag gggaacgcc aatacggcc ttgagcccc 
      ggccaacat ctcctagcg gcgtcagag gccggcgtt 
      atgactttg tttctgacg aatccgttg tgggtcgta 
      aagacgagg ctgtgtttg caatatggg ggttctagt 
      cagcagtac aaggggatg gtcgatgct ttggttaag 
      atctatcgc gcggacggc gttaggggt tattacaaa 
      gggttggtt cctggaatt ttcggggtc tctcatggg 
      gctgtccag tttatggtg tacgagcag ttgaaaaat 
      gagtacacg aaacactac aatgtgccc atttcaacg 
      aagcttgac acagttcag tatctctca tttgcagct 
      ttgtctaaa tttatcgca gcgggggtg acttatcca 
      tatcaggtg gtaagggcg agattgcaa aatcaacat 
      tactcgtac aaagggtcg tttgactgt ataacacag 
      acgtggaaa tatgagggt tggagaggg ttttataaa 
      ggtttaggg acgaatttg ttaagggtt acccctgcc 
      actatgatc acgtttgtc acatacgaa aatgtgtct 
      cactttctc atgaaataa
    mRNA sequence
    • >TC001040 RA
      gcgtttatc acatgcgcg attgtaaca aaacgtcaa 
      taaattcag atgtgtccc taacctcca aattctaat 
      cagataagt taaaattgt gcaatgacc gctcgtaaa 
      aatgagcgt ggtgaccaa aacggccct cgacaccga 
      cgtcagcat cctcaacca catcaaata tgagcactt 
      cgtcgcggg gataagtgg cggcgtcac ctcaaccct 
      gattctgca cccccttga cgttatcaa gattcggtt 
      cgccgtcca cgatggcag gctccaaac cacgccaag 
      gtactcggg gatttggaa cgcattcac gacgatttt 
      tcgccaaga ggggccgcg cgggctcta ccgaggggt 
      tgtccccaa cgtttgggg ggctggcag ctcatgggg 
      gctctattt cctgtttta cacgactat caagaccaa 
      aatacagaa ggggaacgc caatacggc cttgagccc 
      cggccaaca tctcctagc ggcgtcaga ggccggcgt 
      tatgacttt gtttctgac gaatccgtt gtgggtcgt 
      aaagacgag gctgtgttt gcaatatgg gggttctag 
      tcagcagta caaggggat ggtcgatgc tttggttaa 
      gatctatcg cgcggacgg cgttagggg ttattacaa 
      agggttggt tcctggaat tttcggggt ctctcatgg 
      ggctgtcca gtttatggt gtacgagca gttgaaaaa 
      tgagtacac gaaacacta caatgtgcc catttcaac 
      gaagcttga cacagttca gtatctctc atttgcagc 
      tttgtctaa atttatcgc agcgggggt gacttatcc 
      atatcaggt ggtaagggc gagattgca aaatcaaca 
      ttactcgta caaagggtc gtttgactg tataacaca 
      gacgtggaa atatgaggg ttggagagg gttttataa 
      aggtttagg gacgaattt gttaagggt tacccctgc 
      cactatgat cacgtttgt cacatacga aaatgtgtc 
      tcactttct catgaaata attaaaatc atagttaat 
      tattctaga ggttgttgt tatttttta ttgttttag 
      gagttaggc ctgtta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY