Status
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Gene information
General information
TC001033 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 9993610..10007042
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001033
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_07870

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    tropharium size decreased - Number of animals showing the phenotype: 2

    mature egg not present - Number of animals showing the phenotype: 4
    yolk mostly amount decreased - Number of animals showing the phenotype: 2

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature thinner - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature potentially partiallynot present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    legs partially number increased - Penetrance (percentage of animals/eggs on slide): less than 30%

    legs potentially fused - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001033 RA
      MQNVTQESS PDNPKIDKV NVQHEIPST PHSTSNSSP 
      AKSETETYS EHRRYMSDS SESEEDSVS EVESFMIDQ 
      EFEEDTQVV AAKYLLNPD EDRAVDPET QARLEALLQ 
      AAGISQLAA KDGKHLADP EVLRRLTSS VSCALDEAA 
      AALTRMRSD NPRTQPEKI SLVEACTDG DVGTVRKLL 
      TEGRSVHET SEEGESLLS LACSAGYYE LAQVLLAMH 
      ANVEDRGIK GECTPLMEA ASAGHLDIV KLLVKHGAD 
      VNAQSTSGN TPLMYGCAG GHTKVVEFL LEHGANVED 
      HNENGHTPL MEAASAGHV DLAKILLKR GAGINTHSN 
      EFKESALTL ACYKGHLEM VRFLLEAGA DQEHKTDEM 
      HTALMEASM DGHVEVARL LLDSGAQVN MPTDSFESP 
      LTLAACGGH VDLAMLLIE RGANIEEVN DEGYTPLME 
      AAREGHEEM VHLLLGQGA NINAQTDET QETALTLAC 
      CGGFTEVAD ILLKGGADI ELGASTPLM EAAQEGHLD 
      LVKFLLEND ANVHAQTQT GDTALTYAC ENGHTDVAD 
      LLLQYGADL EHESEGGRT PLMKACRAG HLCTVQFLI 
      SKGANVRRQ TTNNDHTPL SLACAGGHL AVVELLLSH 
      NADPCHKLK DNSTMLIEA AKGGHTNVV QLLLDYPHS 
      MQHMMINQH QQGATTATN LSMLPGQVG SPDSPITPT 
      VPPQPINFF QAPLGGALQ EVPEAVRIV SQDDSSPSL 
      DVSKQKSLL AKSRSTGAV FDGTLTSAE AQQVRTQPL 
      PLTDFEEDF LNKEDPNRG ATCLQSPLK LAVSGDTNT 
      FTQNQTQPA FYFTPLTTT VALPTASTT TTQPIKFQS 
      VTSHAEVSQ NTAISDRPK AKPVSKKEG KNVRKQQLQ 
      YQSNLYGQQ SQVFSPIQL QQQQVKSGE KTLNISEGL 
      NVQSLDLDS LDQESLVLK EESHENLLT ASILQQFQN 
      TSIRAAFVG SPQPKTRSD LYDHVQDGK VTKLELSEK 
      DFSTVDDVV QTLESFNTS DISDQSVNQ HNLQVQPYP 
      SAIDETMLA SFGGQQDLQ NQSDLSRVE YFAVPTNGT 
      QVPVHTLYE HYLTTAGMP NKEQSGAIY QAGFQAGIN 
      LMQQQLACP SASTSETTA TVVPTTSIP GTSAQPYIT 
      PQVPCSHVV STAPPQTGH HCQASCTQH QQLVQTHQQ 
      QSHQVQAAT QTTQAGDKK KTVDGMKVK KGRVIRHQN 
      QNVQGNNFH QNQNFQVGT NNAAINQYN GLAPQCQTS 
      LPCLDVDSK TDSNHDTAL TLACAGGHE DLVDLLINR 
      GADIEHRDK KGFTPLILA ATAGHEKVV EVLLNHNAD 
      IEAQSERTK DTPLSLACS GGRYEVVEL LLNRNANKE 
      HRNVSDYTP LSLAASGGY VNIIKLLLS HGAEINSRT 
      GSKLGISPL MLAAMNGHT AAVKLLLDM GSDINAQIE 
      TNRNTALTL ACFQGRHEV VSLLLDRKA NVEHRAKTG 
      LTPLMEAAS GGYVEVGRV LLDKGADVN ATPVPSSRD 
      TALTIAADK GHVRFVELL LCRGAQIEV KNKKGNSPL 
      WLAANGGHL AVVELLYNV NADIDSQDN RKVSCLMAA 
      FRKGHVKVV KWMVHHVTQ FPSDQEMIR YISTVNDKE 
      LLEKCQECV KIIRAAKET QAAKANKNA TILLEELDM 
      EKNREETKR LAAARRRER KKKKKLEKK EERRKLLEE 
      NKKNENYDE KDKKIVEDE KGEEEDNVQ DNIDPAIRN 
      GDSADKEEG DSGIDANSQ GSCSSNDIK PNDKRKDKK 
      KKKANSNSK TSSPSTKAG SSKSQNLTE PALKRNEKN 
      SENKKSAQQ FRKGLVFEA TKSPAERDD FEATGNENY 
      VSNKSKKTH NYDENQTKN GTSTSPKQS SKREEGWKE 
      VVRKSSVQT VSMSESGVK KVSVPLNAI SRVIGRGGS 
      NINAIRGAT GAHIEVEKQ SKGQGERII TIKGAPDVT 
      KQAHSLIAT LIKDPDVDI LQMLPKTNK TTSTTTTLW 
      DKTQIGTKK VAGKVLTVT QPSATTTTQ SKTIQTVAP 
      SVSSTSRSS TTTVKLRGA VPVITSRVT APRLVAQAE 
      KNAVAQIAA VRASAPHTT SSSTNRPTK LITTTATQT 
      FAAKLSETT ANQLSISVK GRNLGLPVT TAGPISPQA 
      IPHSSTNTV TSPKHNRPT PTASAPPNM QHYQSPGKG 
      QIFGSAPPS NVTVPVSEA ARSTTPLTH NIQDEGLQT 
      PSQYSLFTS DQPMWRREN ESQKNNYSS VASGANPQN 
      VIPAKFMDQ DPPQVVDVS KAPGYRGGT VCSPVSSKT 
      SSNSTTPPN ISASSSFQV YPEPAKTQP LPPIGSNIR 
      PQPTSQNDN FYSEFFKSS NIPHMLNDT QLLQSYPKN 
      LNYPQPNMP LASVSRLNP KAPDFSSTV YNAMPKQPQ 
      LYNGFIPSN NALYSGNKG VDSYHRSRW PLMQMQQPF 
      TQQQSELIS GMATGMTLQ TLARVTGSE VLENGPDLG 
      MVNHSPNMS PNLPGMQGE YCEDRKQPQ PIGTERRKY 
      PNIDGCWMY GNEKRWPNN EYGVGRGQV YSEELPQYV 
      DQFPPAVPL ENPHNFSNG PMPYMLQQQ PIGPRPTDF 
      IPVELKDLS WDGSRPNLN DIQEKSQHG WAGMSKWNQ
    CDS sequence
    • >TC001033 RA
      atgcagaat gtcacacaa gaatcgtcg ccagataat 
      ccgaaaata gataaagtg aacgtccag cacgaaata 
      ccgtcgact ccccattca acttccaac tcgagtccc 
      gcaaagtcc gagacggaa acgtattca gaacatcgc 
      cgatatatg tccgactcg tcggaaagc gaagaggac 
      agtgtctca gaggtggag tcatttatg attgatcag 
      gagttcgag gaggatact caagtcgtc gccgctaaa 
      tatcttcta aatccggac gaagatcgc gccgttgat 
      ccggaaact caagctcga cttgaggct ctccttcaa 
      gctgcagga atcagccaa ttagcagcc aaagatggt 
      aaacatcta gcagatccg gaagttttg cgacgttta 
      acatcgagc gtatcgtgt gcattggat gaagcagcc 
      gctgctctg actcgaatg cgttcggat aaccctcgc 
      acccagcca gagaaaatc tctttggtg gaagcttgc 
      accgacggt gatgtcgga actgttaga aagcttcta 
      acagaaggt agatccgta cacgaaacg tctgaagaa 
      ggagagagt ctcttatcg ctcgcgtgc agtgctgga 
      tattatgaa ctagcgcag gttttacta gcgatgcat 
      gcaaacgtc gaagaccga ggcatcaaa ggagaatgc 
      actcctttg atggaagcg gcatcagcc ggccatctc 
      gacatcgtc aaactccta gtgaaacac ggcgccgac 
      gtcaacgcg caatccacc tccggcaac accccactc 
      atgtacggc tgtgccggc ggtcacacg aaagtcgtc 
      gagttcctc ctggaacac ggcgctaac gtcgaagac 
      cacaatgaa aacgggcac acgcccctc atggaggct 
      gccagcgcc ggccacgtc gatctcgcg aaaattctc 
      ctgaagaga ggcgccgga atcaacact cattcgaac 
      gagtttaaa gaatcggcg ctgacgctt gcgtgttat 
      aaaggacat ttagaaatg gtgaggttt ttgttagag 
      gccggagcc gatcaagaa cacaaaacg gatgaaatg 
      cacaccgca ttgatggaa gcatcgatg gatgggcac 
      gtcgaggtg gctcggctc ttgctcgat tcaggggct 
      caggttaac atgccgacg gacagcttc gagtcaccg 
      ttaacgtta gcggcgtgt gggggccac gtggatttg 
      gccatgttg ctcatagag aggggggct aacatcgag 
      gaggtcaac gatgagggg tacacgccg ctgatggaa 
      gccgcgagg gagggccac gaggaaatg gtccatttg 
      ttgctggga caaggggcg aacatcaat gctcagact 
      gatgaaaca caggagaca gccttgact ttggcttgt 
      tgtgggggt ttcaccgag gtcgcggat atcctcttg 
      aaagggggc gccgatatc gagctaggg gcgtctacg 
      ccgctgatg gaagcggca caagagggc catttggac 
      ctggtgaag tttctgttg gagaacgac gccaatgtt 
      cacgctcag acacaaacc ggcgataca gcgttgaca 
      tacgcttgc gagaatggt cacaccgac gtcgccgac 
      ctcctcctg caatacggc gccgacttg gagcacgag 
      tctgaggga ggtcggacg ccgttgatg aaggcgtgt 
      cgtgccggt cacttgtgc actgtgcag tttttaata 
      tctaaaggt gcgaatgtg aggagacaa acgacgaat 
      aatgatcac acaccgttg tcgttggcg tgcgccggg 
      ggtcattta gctgtggtg gagctgttg ttgagtcat 
      aatgccgac ccgtgtcac aaattgaag gataattcc 
      accatgttg attgaagcg gccaaagga ggacatacg 
      aacgtagtg caattactt ttagattat ccgcattct 
      atgcagcat atgatgatc aatcaacat caacaaggg 
      gctacgacg gctaccaat ctgtccatg cttccagga 
      caagttggt agccctgat tcgccgatc acacccacc 
      gtgcccccg caacccatc aactttttc caagcacct 
      ctaggcggc gctctgcag gaagttccc gaagccgtt 
      cggatagtc tctcaggac gattcgagt ccgtcgctg 
      gacgtatcc aaacaaaaa tccctattg gcgaaaagt 
      cgttcaacc ggcgctgtc ttcgacggt accttaact 
      tcggctgaa gcccaacaa gttcgaaca caaccgtta 
      cctttgacc gatttcgaa gaggatttc ctcaacaaa 
      gaagatcca aacagagga gcgacgtgt ctacagtca 
      cctctcaaa ctagctgta agcggagat acgaataca 
      ttcactcaa aatcagacc caaccggcg ttttatttt 
      acgccgttg acgaccacc gtcgcgtta cccactgcc 
      agtacgacc accactcaa ccgatcaaa tttcaaagt 
      gttacgtca catgcagaa gttagtcag aacacggcg 
      atcagtgat cgaccgaag gcgaagccg gtctcaaaa 
      aaagaaggc aagaatgtc agaaaacaa caattacaa 
      taccaatcg aatttatac gggcaacaa agtcaggtg 
      ttcagtcca attcagttg caacagcaa caagtcaag 
      tctggtgaa aagacttta aatatttcg gaaggtctg 
      aatgtgcaa agtttagat ttggactct ttagatcag 
      gagtcatta gtgttgaaa gaggaaagt cacgagaat 
      cttttaacg gcttcgatt ttacaacag tttcaaaat 
      acgtcgatt agagcagct ttcgtcgga tcaccgcaa 
      ccgaaaacg cgttcagat ctctacgat cacgtacaa 
      gatggtaaa gtgacaaaa ttagaacta tccgagaaa 
      gatttttct acagtggat gatgtcgtt caaacgctt 
      gaatctttt aacacatct gatatcagc gaccaaagt 
      gtaaatcaa cataatctt caggtacaa ccctatcct 
      agcgctatc gacgaaaca atgctggcc tccttcggc 
      ggccaacag gacctgcaa aaccagagc gacttatcc 
      cgtgtggaa tacttcgct gtgcccacc aacggcacc 
      caagtccca gttcacacc ctttacgag cactacctg 
      accactgca ggtatgcca aacaaagaa caaagcggt 
      gctatctac caagccgga tttcaagct ggaattaat 
      ctaatgcag caacaatta gcatgtccg agtgcaagc 
      acgagcgag acaaccgcg actgtggta ccaacaacc 
      tcaataccg ggtacgtcc gcacagccc tatattaca 
      ccacaggtg ccctgcagt cacgtggtg agcactgcg 
      cccccacaa acaggacat cattgtcaa gcctcgtgc 
      acgcaacac caacaatta gttcagacg caccaacag 
      caaagtcat caagtgcaa gctgcaaca cagacgacc 
      caagccggc gataagaag aaaacagtc gatgggatg 
      aaagtgaag aagggacgg gtaatcagg catcagaat 
      cagaacgtg caagggaat aatttccac cagaatcag 
      aattttcaa gttgggaca aataacgca gctataaac 
      cagtacaac ggcttggcc ccccaatgt caaacctcc 
      ttgccatgc ctcgacgta gactccaaa accgacagc 
      aaccacgac accgctcta accctcgct tgtgccggc 
      ggccacgaa gacctggtc gacctcctc attaatcgt 
      ggagctgac atcgaacac cgcgacaag aagggattc 
      actccgttg atcctcgct gccaccgcc ggtcacgag 
      aaggtcgtt gaagtcctt ctcaatcat aacgccgac 
      atcgaggcg caatccgag cgaacaaaa gacacacct 
      ttgagtctc gcctgttcg ggaggacga tacgaagtc 
      gtcgaactc cttctcaat aggaacgcc aacaaggaa 
      cacagaaat gtctccgat tatactccg ttgagtttg 
      gccgcttca ggtggttat gtcaacatt ataaagttg 
      cttttgagt catggagct gagattaat tcacgcacg 
      gggagcaag ttgggaatt tcgcccctt atgctagcg 
      gcaatgaat ggacacacc gccgctgtg aaactgttg 
      ctggatatg ggaagtgat atcaatgct caaatcgag 
      accaatagg aacacggcg ctgactttg gcctgcttt 
      caagggagg cacgaagtg gtcagcttg ctgctagat 
      cggaaggct aatgttgaa cacagagct aagactggc 
      ctcacgccg ctcatggaa gctgcgagc ggaggttat 
      gtagaagtc ggccgagtc ctcctcgac aaaggcgcc 
      gatgtgaac gcgactcca gttccttcg tcacgtgac 
      accgctctc accatagcc gccgataaa gggcacgtc 
      cgtttcgta gaacttctt ctctgtaga ggcgctcaa 
      atcgaagtt aagaacaag aaaggcaat tctccgctt 
      tggttggcc gcaaacggt ggtcattta gccgtcgtt 
      gagttatta tacaatgtc aatgcagac attgactcg 
      caagacaat agaaaagtc tcctgcttg atggcggcg 
      tttcgaaaa ggtcacgtt aaagtggtt aagtggatg 
      gtacatcac gtgacacaa tttccgagc gatcaagaa 
      atgatccgc tatatttcg accgtcaac gataaagag 
      ttgttagag aaatgccaa gagtgtgtg aagattatt 
      cgagccgcc aaagagaca caggcggct aaggcgaat 
      aaaaatgcg acgattttg ttagaagag ttggatatg 
      gagaaaaat cgtgaggag acaaagaga ctcgctgct 
      gcgcgaaga agggaacga aagaagaaa aagaaactt 
      gaaaagaag gaagagagg cgtaagtta ctcgaggag 
      aacaaaaag aacgagaat tatgatgag aaagataag 
      aaaatcgtc gaggatgaa aaaggcgaa gaggaggat 
      aacgtccaa gataacatc gatccggca attcgtaac 
      ggcgattcc gccgataaa gaagagggt gatagtgga 
      atagatgcc aacagccaa ggcagttgc tcaagtaac 
      gatatcaaa ccgaacgat aagcgcaaa gacaaaaag 
      aaaaagaaa gctaattcg aattcgaaa accagcagt 
      ccttcgacc aaagccggt tcgagtaaa tcacaaaat 
      ctaaccgaa ccagctctc aaacgtaac gagaaaaat 
      tcagaaaac aaaaaatca gctcaacaa ttcagaaag 
      ggtcttgtt tttgaagcg acgaaaagt ccagctgaa 
      cgtgacgat ttcgaagct acaggaaac gaaaactac 
      gtttcaaac aagtcgaaa aagacacac aattatgat 
      gaaaatcaa actaaaaac ggcacatct accagtccg 
      aaacagtcg tcgaaacga gaggaaggc tggaaggaa 
      gttgtgagg aagtcgtca gtgcagacg gtttcgatg 
      tccgaatcc ggggttaag aaagtctca gtaccgtta 
      aatgcgatt tcgcgagtt atcggacga ggtgggagt 
      aatattaac gcaattagg ggcgctaca ggggcacac 
      atagaagtg gaaaagcag agtaaaggg caaggggaa 
      aggattatt acgattaag ggcgcaccg gatgttaca 
      aaacaagca cattcgtta attgcgacg ctgattaaa 
      gatcccgat gtagatatt ttgcaaatg cttccgaaa 
      acgaacaaa acaacgtcg actacgact acattgtgg 
      gacaagact caaatcggg acgaaaaaa gttgccggc 
      aaagtgttg accgtgact caaccatca gcaaccaca 
      acgacccaa tcaaaaacc atccaaact gtcgcacct 
      tcggtgagt tcaacgtcg cgttcgtct acgacaact 
      gtaaaactc cgcggagcc gtccctgta ataacatca 
      cgcgtcaca gccccacgt ctagtagcc caagccgag 
      aaaaacgcc gttgctcaa atcgcagca gtccgtgct 
      tcagcccct cacaccacc tcatcatca acgaatcgc 
      ccaactaaa ctaataaca acaacagcg acacaaacc 
      ttcgccgca aaactctcc gaaacaaca gccaatcaa 
      ctgtcgatt tcggtcaag ggtcgaaac ttggggctt 
      ccggtgacc accgccggt ccaatttcc ccacaagcg 
      atacctcac agttctact aacacagtc acctcaccc 
      aaacacaac cgaccgacc ccgacagct tcagcaccg 
      ccgaatatg caacactac caaagtccg ggaaaagga 
      caaattttc ggcagtgcc cccccgagt aatgtcaca 
      gttccggtt tcagaagcg gctcgaagt acaacaccc 
      ttgactcac aacatacaa gacgagggt ttgcaaact 
      ccttcgcaa tattcgtta tttacgtca gatcagccg 
      atgtggagg cgcgaaaac gaatctcag aagaacaat 
      tattcgtca gttgcgagc ggtgccaat ccacaaaac 
      gtaatcccg gccaaattc atggatcaa gacccacct 
      caagtggtg gacgtgtct aaagcacca ggctatcga 
      ggcggaacg gtctgttct cccgtttcc tccaaaacc 
      agtagcaac agtaccaca cctcctaac atctccgcc 
      agttcctcc tttcaagtg taccccgaa ccggccaaa 
      acccaaccc ctccccccc atcgggagc aacatccgc 
      ccgcaaccg acctcccaa aacgacaac ttctactcc 
      gaattcttc aaatcgagc aacattcct cacatgctc 
      aacgacact caactgcta caatcctac cctaaaaac 
      ctcaactac ccccaacct aacatgccc ctagcaagt 
      gtctcgcgt cttaatccc aaagcgccc gatttttcg 
      agcactgtg tacaacgcc atgcctaag caaccccaa 
      ctgtataac ggattcatc ccgagcaac aacgccctc 
      tattccggg aataaaggc gtggattcg taccatagg 
      tcgcgatgg cctctcatg cagatgcaa cagccgttc 
      actcaacaa cagagcgaa ttgatcagt gggatggcc 
      accgggatg acgttgcag accttggcg agggtcacc 
      gggagtgag gtcttggaa aatggaccc gatttgggg 
      atggtaaac cactcgccg aatatgtcg ccgaatttg 
      ccagggatg caaggggag tattgtgag gatcggaag 
      cagccacaa ccgatcggg acggaaagg aggaagtat 
      ccgaatatt gacgggtgc tggatgtac gggaacgag 
      aagaggtgg ccaaacaat gagtacgga gtggggaga 
      ggccaggtg tattccgaa gagttgccg cagtatgtt 
      gatcagttt ccgcctgcg gtgcctttg gagaacccc 
      cacaatttc tcgaacgga ccaatgccc tatatgttg 
      caacagcag ccgataggg ccgcgccca actgatttc 
      atcccggtg gaattgaaa gacttgtct tgggacggt 
      agccgaccg aatttgaac gatattcaa gagaaatcg 
      caacacggt tgggcggga atgtcgaaa tggaaccag 
      taa
    mRNA sequence
    • >TC001033 RA
      tctcgccat agtagaaaa tggcgaccg tgatcattt 
      tgatgtaac gaatgtact ttttgaagt gcgtgtagt 
      gaatctagt aaaataatc gcagcagtt tcgttgtgt 
      ttcgtttga atgaaaggc aaacatgca gaatgtcac 
      acaagaatc gtcgccaga taatccgaa aatagataa 
      agtgaacgt ccagcacga aataccgtc gactcccca 
      ttcaacttc caactcgag tcccgcaaa gtccgagac 
      ggaaacgta ttcagaaca tcgccgata tatgtccga 
      ctcgtcgga aagcgaaga ggacagtgt ctcagaggt 
      ggagtcatt tatgattga tcaggagtt cgaggagga 
      tactcaagt cgtcgccgc taaatatct tctaaatcc 
      ggacgaaga tcgcgccgt tgatccgga aactcaagc 
      tcgacttga ggctctcct tcaagctgc aggaatcag 
      ccaattagc agccaaaga tggtaaaca tctagcaga 
      tccggaagt tttgcgacg tttaacatc gagcgtatc 
      gtgtgcatt ggatgaagc agccgctgc tctgactcg 
      aatgcgttc ggataaccc tcgcaccca gccagagaa 
      aatctcttt ggtggaagc ttgcaccga cggtgatgt 
      cggaactgt tagaaagct tctaacaga aggtagatc 
      cgtacacga aacgtctga agaaggaga gagtctctt 
      atcgctcgc gtgcagtgc tggatatta tgaactagc 
      gcaggtttt actagcgat gcatgcaaa cgtcgaaga 
      ccgaggcat caaaggaga atgcactcc tttgatgga 
      agcggcatc agccggcca tctcgacat cgtcaaact 
      cctagtgaa acacggcgc cgacgtcaa cgcgcaatc 
      cacctccgg caacacccc actcatgta cggctgtgc 
      cggcggtca cacgaaagt cgtcgagtt cctcctgga 
      acacggcgc taacgtcga agaccacaa tgaaaacgg 
      gcacacgcc cctcatgga ggctgccag cgccggcca 
      cgtcgatct cgcgaaaat tctcctgaa gagaggcgc 
      cggaatcaa cactcattc gaacgagtt taaagaatc 
      ggcgctgac gcttgcgtg ttataaagg acatttaga 
      aatggtgag gtttttgtt agaggccgg agccgatca 
      agaacacaa aacggatga aatgcacac cgcattgat 
      ggaagcatc gatggatgg gcacgtcga ggtggctcg 
      gctcttgct cgattcagg ggctcaggt taacatgcc 
      gacggacag cttcgagtc accgttaac gttagcggc 
      gtgtggggg ccacgtgga tttggccat gttgctcat 
      agagagggg ggctaacat cgaggaggt caacgatga 
      ggggtacac gccgctgat ggaagccgc gagggaggg 
      ccacgagga aatggtcca tttgttgct gggacaagg 
      ggcgaacat caatgctca gactgatga aacacagga 
      gacagcctt gactttggc ttgttgtgg gggtttcac 
      cgaggtcgc ggatatcct cttgaaagg gggcgccga 
      tatcgagct aggggcgtc tacgccgct gatggaagc 
      ggcacaaga gggccattt ggacctggt gaagtttct 
      gttggagaa cgacgccaa tgttcacgc tcagacaca 
      aaccggcga tacagcgtt gacatacgc ttgcgagaa 
      tggtcacac cgacgtcgc cgacctcct cctgcaata 
      cggcgccga cttggagca cgagtctga gggaggtcg 
      gacgccgtt gatgaaggc gtgtcgtgc cggtcactt 
      gtgcactgt gcagttttt aatatctaa aggtgcgaa 
      tgtgaggag acaaacgac gaataatga tcacacacc 
      gttgtcgtt ggcgtgcgc cgggggtca tttagctgt 
      ggtggagct gttgttgag tcataatgc cgacccgtg 
      tcacaaatt gaaggataa ttccaccat gttgattga 
      agcggccaa aggaggaca tacgaacgt agtgcaatt 
      acttttaga ttatccgca ttctatgca gcatatgat 
      gatcaatca acatcaaca aggggctac gacggctac 
      caatctgtc catgcttcc aggacaagt tggtagccc 
      tgattcgcc gatcacacc caccgtgcc cccgcaacc 
      catcaactt tttccaagc acctctagg cggcgctct 
      gcaggaagt tcccgaagc cgttcggat agtctctca 
      ggacgattc gagtccgtc gctggacgt atccaaaca 
      aaaatccct attggcgaa aagtcgttc aaccggcgc 
      tgtcttcga cggtacctt aacttcggc tgaagccca 
      acaagttcg aacacaacc gttaccttt gaccgattt 
      cgaagagga tttcctcaa caaagaaga tccaaacag 
      aggagcgac gtgtctaca gtcacctct caaactagc 
      tgtaagcgg agatacgaa tacattcac tcaaaatca 
      gacccaacc ggcgtttta ttttacgcc gttgacgac 
      caccgtcgc gttacccac tgccagtac gaccaccac 
      tcaaccgat caaatttca aagtgttac gtcacatgc 
      agaagttag tcagaacac ggcgatcag tgatcgacc 
      gaaggcgaa gccggtctc aaaaaaaga aggcaagaa 
      tgtcagaaa acaacaatt acaatacca atcgaattt 
      atacgggca acaaagtca ggtgttcag tccaattca 
      gttgcaaca gcaacaagt caagtctgg tgaaaagac 
      tttaaatat ttcggaagg tctgaatgt gcaaagttt 
      agatttgga ctctttaga tcaggagtc attagtgtt 
      gaaagagga aagtcacga gaatctttt aacggcttc 
      gattttaca acagtttca aaatacgtc gattagagc 
      agctttcgt cggatcacc gcaaccgaa aacgcgttc 
      agatctcta cgatcacgt acaagatgg taaagtgac 
      aaaattaga actatccga gaaagattt ttctacagt 
      ggatgatgt cgttcaaac gcttgaatc ttttaacac 
      atctgatat cagcgacca aagtgtaaa tcaacataa 
      tcttcaggt acaacccta tcctagcgc tatcgacga 
      aacaatgct ggcctcctt cggcggcca acaggacct 
      gcaaaacca gagcgactt atcccgtgt ggaatactt 
      cgctgtgcc caccaacgg cacccaagt cccagttca 
      cacccttta cgagcacta cctgaccac tgcaggtat 
      gccaaacaa agaacaaag cggtgctat ctaccaagc 
      cggatttca agctggaat taatctaat gcagcaaca 
      attagcatg tccgagtgc aagcacgag cgagacaac 
      cgcgactgt ggtaccaac aacctcaat accgggtac 
      gtccgcaca gccctatat tacaccaca ggtgccctg 
      cagtcacgt ggtgagcac tgcgccccc acaaacagg 
      acatcattg tcaagcctc gtgcacgca acaccaaca 
      attagttca gacgcacca acagcaaag tcatcaagt 
      gcaagctgc aacacagac gacccaagc cggcgataa 
      gaagaaaac agtcgatgg gatgaaagt gaagaaggg 
      acgggtaat caggcatca gaatcagaa cgtgcaagg 
      gaataattt ccaccagaa tcagaattt tcaagttgg 
      gacaaataa cgcagctat aaaccagta caacggctt 
      ggcccccca atgtcaaac ctccttgcc atgcctcga 
      cgtagactc caaaaccga cagcaacca cgacaccgc 
      tctaaccct cgcttgtgc cggcggcca cgaagacct 
      ggtcgacct cctcattaa tcgtggagc tgacatcga 
      acaccgcga caagaaggg attcactcc gttgatcct 
      cgctgccac cgccggtca cgagaaggt cgttgaagt 
      ccttctcaa tcataacgc cgacatcga ggcgcaatc 
      cgagcgaac aaaagacac acctttgag tctcgcctg 
      ttcgggagg acgatacga agtcgtcga actccttct 
      caataggaa cgccaacaa ggaacacag aaatgtctc 
      cgattatac tccgttgag tttggccgc ttcaggtgg 
      ttatgtcaa cattataaa gttgctttt gagtcatgg 
      agctgagat taattcacg cacggggag caagttggg 
      aatttcgcc ccttatgct agcggcaat gaatggaca 
      caccgccgc tgtgaaact gttgctgga tatgggaag 
      tgatatcaa tgctcaaat cgagaccaa taggaacac 
      ggcgctgac tttggcctg ctttcaagg gaggcacga 
      agtggtcag cttgctgct agatcggaa ggctaatgt 
      tgaacacag agctaagac tggcctcac gccgctcat 
      ggaagctgc gagcggagg ttatgtaga agtcggccg 
      agtcctcct cgacaaagg cgccgatgt gaacgcgac 
      tccagttcc ttcgtcacg tgacaccgc tctcaccat 
      agccgccga taaagggca cgtccgttt cgtagaact 
      tcttctctg tagaggcgc tcaaatcga agttaagaa 
      caagaaagg caattctcc gctttggtt ggccgcaaa 
      cggtggtca tttagccgt cgttgagtt attatacaa 
      tgtcaatgc agacattga ctcgcaaga caatagaaa 
      agtctcctg cttgatggc ggcgtttcg aaaaggtca 
      cgttaaagt ggttaagtg gatggtaca tcacgtgac 
      acaatttcc gagcgatca agaaatgat ccgctatat 
      ttcgaccgt caacgataa agagttgtt agagaaatg 
      ccaagagtg tgtgaagat tattcgagc cgccaaaga 
      gacacaggc ggctaaggc gaataaaaa tgcgacgat 
      tttgttaga agagttgga tatggagaa aaatcgtga 
      ggagacaaa gagactcgc tgctgcgcg aagaaggga 
      acgaaagaa gaaaaagaa acttgaaaa gaaggaaga 
      gaggcgtaa gttactcga ggagaacaa aaagaacga 
      gaattatga tgagaaaga taagaaaat cgtcgagga 
      tgaaaaagg cgaagagga ggataacgt ccaagataa 
      catcgatcc ggcaattcg taacggcga ttccgccga 
      taaagaaga gggtgatag tggaataga tgccaacag 
      ccaaggcag ttgctcaag taacgatat caaaccgaa 
      cgataagcg caaagacaa aaagaaaaa gaaagctaa 
      ttcgaattc gaaaaccag cagtccttc gaccaaagc 
      cggttcgag taaatcaca aaatctaac cgaaccagc 
      tctcaaacg taacgagaa aaattcaga aaacaaaaa 
      atcagctca acaattcag aaagggtct tgtttttga 
      agcgacgaa aagtccagc tgaacgtga cgatttcga 
      agctacagg aaacgaaaa ctacgtttc aaacaagtc 
      gaaaaagac acacaatta tgatgaaaa tcaaactaa 
      aaacggcac atctaccag tccgaaaca gtcgtcgaa 
      acgagagga aggctggaa ggaagttgt gaggaagtc 
      gtcagtgca gacggtttc gatgtccga atccggggt 
      taagaaagt ctcagtacc gttaaatgc gatttcgcg 
      agttatcgg acgaggtgg gagtaatat taacgcaat 
      taggggcgc tacaggggc acacataga agtggaaaa 
      gcagagtaa agggcaagg ggaaaggat tattacgat 
      taagggcgc accggatgt tacaaaaca agcacattc 
      gttaattgc gacgctgat taaagatcc cgatgtaga 
      tattttgca aatgcttcc gaaaacgaa caaaacaac 
      gtcgactac gactacatt gtgggacaa gactcaaat 
      cgggacgaa aaaagttgc cggcaaagt gttgaccgt 
      gactcaacc atcagcaac cacaacgac ccaatcaaa 
      aaccatcca aactgtcgc accttcggt gagttcaac 
      gtcgcgttc gtctacgac aactgtaaa actccgcgg 
      agccgtccc tgtaataac atcacgcgt cacagcccc 
      acgtctagt agcccaagc cgagaaaaa cgccgttgc 
      tcaaatcgc agcagtccg tgcttcagc ccctcacac 
      cacctcatc atcaacgaa tcgcccaac taaactaat 
      aacaacaac agcgacaca aaccttcgc cgcaaaact 
      ctccgaaac aacagccaa tcaactgtc gatttcggt 
      caagggtcg aaacttggg gcttccggt gaccaccgc 
      cggtccaat ttccccaca agcgatacc tcacagttc 
      tactaacac agtcacctc acccaaaca caaccgacc 
      gaccccgac agcttcagc accgccgaa tatgcaaca 
      ctaccaaag tccgggaaa aggacaaat tttcggcag 
      tgccccccc gagtaatgt cacagttcc ggtttcaga 
      agcggctcg aagtacaac acccttgac tcacaacat 
      acaagacga gggtttgca aactccttc gcaatattc 
      gttatttac gtcagatca gccgatgtg gaggcgcga 
      aaacgaatc tcagaagaa caattattc gtcagttgc 
      gagcggtgc caatccaca aaacgtaat cccggccaa 
      attcatgga tcaagaccc acctcaagt ggtggacgt 
      gtctaaagc accaggcta tcgaggcgg aacggtctg 
      ttctcccgt ttcctccaa aaccagtag caacagtac 
      cacacctcc taacatctc cgccagttc ctcctttca 
      agtgtaccc cgaaccggc caaaaccca acccctccc 
      ccccatcgg gagcaacat ccgcccgca accgacctc 
      ccaaaacga caacttcta ctccgaatt cttcaaatc 
      gagcaacat tcctcacat gctcaacga cactcaact 
      gctacaatc ctaccctaa aaacctcaa ctaccccca 
      acctaacat gcccctagc aagtgtctc gcgtcttaa 
      tcccaaagc gcccgattt ttcgagcac tgtgtacaa 
      cgccatgcc taagcaacc ccaactgta taacggatt 
      catcccgag caacaacgc cctctattc cgggaataa 
      aggcgtgga ttcgtacca taggtcgcg atggcctct 
      catgcagat gcaacagcc gttcactca acaacagag 
      cgaattgat cagtgggat ggccaccgg gatgacgtt 
      gcagacctt ggcgagggt caccgggag tgaggtctt 
      ggaaaatgg acccgattt ggggatggt aaaccactc 
      gccgaatat gtcgccgaa tttgccagg gatgcaagg 
      ggagtattg tgaggatcg gaagcagcc acaaccgat 
      cgggacgga aaggaggaa gtatccgaa tattgacgg 
      gtgctggat gtacgggaa cgagaagag gtggccaaa 
      caatgagta cggagtggg gagaggcca ggtgtattc 
      cgaagagtt gccgcagta tgttgatca gtttccgcc 
      tgcggtgcc tttggagaa cccccacaa tttctcgaa 
      cggaccaat gccctatat gttgcaaca gcagccgat 
      agggccgcg cccaactga tttcatccc ggtggaatt 
      gaaagactt gtcttggga cggtagccg accgaattt 
      gaacgatat tcaagagaa atcgcaaca cggttgggc 
      gggaatgtc gaaatggaa ccagtaatc actgactat 
      gacccaaac agaactata agcattttt gacaaagtc 
      catcgtgcg aaatttagc ttacatctt tgtgatctt 
      tgggataca agttttaca gtagaacct agtactagt 
      ttttgttta tggtatctc tcttgaagt acactcacg 
      tcatgtgca agtgtctgg ttaggaggc ccattatac 
      aggccacga gcccccttg cctgcattc ggtgtatat 
      aaggaaaaa acctattca gatcattat tctaattgt 
      agtatgttg tttgcatgt cccattatt tggttttta 
      actgcttat agtttacga aataaactg ttttacttg 
      attatcttt tgtagccga tttggatca tgaatgata 
      cagcttcac tagtggctc caaaaacaa gccgattgt 
      cgagctgaa gttagaata tataattat aacataaca 
      ctatgtata atgtagtag ttgcgcaat ggtgtcgag 
      ggattggcg atagggaca agtgttaac ttgtgaata 
      atttgttct ctattcgaa tatttactt tctttttct 
      tacatgtgt aagcgcagt gtttgtatg cctcacgtc 
      tgggctaca gcccggcac aagcactcg gtgggtatc 
      gctaactcc cacgaaact gtagggtta ttattatac 
      ggatcatta tgtaatatt gcttgtaca tgaagactt 
      aaatatatc tttatgcac tgt
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