Status
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Gene information
General information
TC032124 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 9262201..9264526
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC032124
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_07862

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    Fertility
    mature egg not present - Number of animals showing the phenotype: 3
    vitellogenic egg chamber number decreased - Number of animals showing the phenotype: 4
    previtellogenic egg chamber not present - Number of animals showing the phenotype: 4
    tropharium structure irregular - Number of animals showing the phenotype: 4

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    larva segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head appendages unilateral mostlynot present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head capsule size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    prothorax potentially not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    urogomphi potentially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen tracheal opening size irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032124 RA
      MTHLVLVLV LVAAVRTGA CPVMCTCSR HLAVRCTQQ 
VPAEIFTNL TNLQELVIE NSDLGDLSN VSVADLELQ 
SMRLSQNRI VSLPELNVT NLRHLDLSG NLIETLGVE 
FRTLRSLEI LNLSANFLT VIDSDSFSG LESLKCLDL 
SRNNLTRLD DALFEPLQA LQYLNLSSN QLTILNERC 
FISLVKLQQ LDVSFNRLV RVELGSLQL PSLARLLLA 
GNSRLGASK QPMLVGTGQ RLQTVDASN IGLEQVPPA 
LTHSIRTLR LVGNSIKTV RCGDLDSYP LLQLLDFDS 
NGLEVIEDD ALGRLDSLT ILYLTNNNM SEIPKSLSE 
KLKVLHLEH NQIQQVRTR DLQGLTSLE VLLLNDNKI 
RLIEAEAFN QLTSLVTLD ISRNPIKIL EAGCLSGSM 
ALQVLRLSN IDVDSPPKE VSFPLSSTE HLITLDLSG 
SPGLARQLL VDTAALAAS RQLQELDLS FTDLEFIRS 
DLLHFLPQL RIFHIDGNR INCTQLEWL AAWMRRQDE 
PEYKRVTCV SPPELWGTL LIDLQPVEA TPNRPTTTQ 
TPTTQMEDY IDIESFLRE NKSEIFPIT NSTRGEMSM 
FSAGAQERL HPGLVLAAA MLGTALVLV LLGVRFAKR 
RRDALRLRQ EDLEVSSLP GVTELW
    CDS sequence
    • >TC032124 RA
      atgacgcac ctggtgcta gttcttgtg cttgttgcc 
gctgtccgg acgggtgcg tgtcccgtc atgtgcacg 
tgttcccga cacctcgcc gttcgctgc acccaacaa 
gtgcccgct gagattttc accaatctg accaacctc 
caagagctg gtgatcgag aacagcgac ctcggggat 
ttgtccaat gtgtcggtc gccgattta gaattacag 
tcgatgcga ctgtcgcag aatcgtatt gtgtctctc 
ccggaattg aacgtcacg aatttgaga cacttggac 
ttgtcgggg aatttgatc gagacgctc ggagtggag 
tttaggaca ttacggagt ctggagata ctcaatttg 
agtgcaaac tttttaaca gtgattgat tcggacagt 
tttagtgga cttgaatcc ttgaagtgt ttagatttg 
tctcggaat aatctgaca cggctcgat gacgcgctt 
tttgaaccg ttgcaagcg ttgcaatat ctcaaccta 
agctccaat caattgacg attcttaac gagcggtgt 
tttatcagt ttagtgaaa ctccaacaa cttgatgtt 
tctttcaat cgcttggtt cgggtcgaa ttggggagt 
ttgcaattg ccgagtctg gcgagactt ctcttagct 
ggtaattcg agactaggg gcttcgaaa caaccaatg 
cttgtcggg actggtcaa agactccaa actgttgat 
gcttcaaac atcgggctt gaacaagta cccccagct 
ttgacccat tcgattcga acgcttcgc ttagttggt 
aatagcata aagacggtc cggtgtggt gacctcgac 
tcttatccc ctgttacaa ttgctcgat ttcgattcg 
aacggcttg gaagtgatc gaagacgac gctttgggt 
cgtctcgac tccctcacg atcctctac ttgactaat 
aacaacatg agcgaaatc ccgaaaagt ctctcggag 
aagctcaaa gtccttcat ttggaacac aatcaaatc 
caacaagtg cgtactaga gacctccaa ggcttgacc 
tctctcgaa gtccttctc ctcaacgac aataaaatc 
cgtttgatc gaagcggaa gccttcaac cagttgacg 
tctcttgtc actcttgat atctcccgt aaccctatt 
aaaatcctc gaagccggt tgtttgtcc ggctcgatg 
gccctccaa gtcctccgt ttatccaac atcgatgtc 
gattcacca ccgaaagaa gtttccttc cccttatcg 
tcaaccgag cacctgatc acactcgat ctctccgga 
agcccggga cttgcccga caattgcta gtcgatact 
gcagctcta gccgcctcg cgacaatta caagagctt 
gatttgtct ttcaccgac ctcgagttc atccgctct 
gacttgctc cactttttg ccccaattg cggatcttc 
cacattgac ggcaaccga atcaactgc acgcagctc 
gagtggttg gcggcttgg atgcggcgc caggacgag 
cccgagtac aaaagagtg acatgtgtg agcccccct 
gagctgtgg gggaccctc ctcatcgac ctccaaccg 
gtggaagcg accccaaac cgccccaca accacacaa 
acacccacg acacagatg gaggactac atcgacata 
gagtcgttt ctccgggaa aacaaatcg gaaattttc 
ccaattact aactccacg cgtggagaa atgtccatg 
tttagtgct ggggcgcag gagaggctg cacccgggg 
ctggtcctg gccgctgcc atgctagga acagcccta 
gtgctggtt ctactgggg gtgaggttt gccaaacgg 
aggagggac gcattgagg ctcagacag gaggacctc 
gaggtcagc tcgctgcct ggcgttacg gaactatgg 
tga
    mRNA sequence
    • >TC032124 RA
      cgccagatg ccggccggt ccaccaacg ggcatgtga 
ccgcaaaga gatgacgca cctggtgct agttcttgt 
gcttgttgc cgctgtccg gacgggtgc gtgtcccgt 
catgtgcac gtgttcccg acacctcgc cgttcgctg 
cacccaaca agtgcccgc tgagatttt caccaatct 
gaccaacct ccaagagct ggtgatcga gaacagcga 
cctcgggga tttgtccaa tgtgtcggt cgccgattt 
agaattaca gtcgatgcg actgtcgca gaatcgtat 
tgtgtctct cccggaatt gaacgtcac gaatttgag 
acacttgga cttgtcggg gaatttgat cgagacgct 
cggagtgga gtttaggac attacggag tctggagat 
actcaattt gagtgcaaa ctttttaac agtgattga 
ttcggacag ttttagtgg acttgaatc cttgaagtg 
tttagattt gtctcggaa taatctgac acggctcga 
tgacgcgct ttttgaacc gttgcaagc gttgcaata 
tctcaacct aagctccaa tcaattgac gattcttaa 
cgagcggtg ttttatcag tttagtgaa actccaaca 
acttgatgt ttctttcaa tcgcttggt tcgggtcga 
attggggag tttgcaatt gccgagtct ggcgagact 
tctcttagc tggtaattc gagactagg ggcttcgaa 
acaaccaat gcttgtcgg gactggtca aagactcca 
aactgttga tgcttcaaa catcgggct tgaacaagt 
acccccagc tttgaccca ttcgattcg aacgcttcg 
cttagttgg taatagcat aaagacggt ccggtgtgg 
tgacctcga ctcttatcc cctgttaca attgctcga 
tttcgattc gaacggctt ggaagtgat cgaagacga 
cgctttggg tcgtctcga ctccctcac gatcctcta 
cttgactaa taacaacat gagcgaaat cccgaaaag 
tctctcgga gaagctcaa agtccttca tttggaaca 
caatcaaat ccaacaagt gcgtactag agacctcca 
aggcttgac ctctctcga agtccttct cctcaacga 
caataaaat ccgtttgat cgaagcgga agccttcaa 
ccagttgac gtctcttgt cactcttga tatctcccg 
taaccctat taaaatcct cgaagccgg ttgtttgtc 
cggctcgat ggccctcca agtcctccg tttatccaa 
catcgatgt cgattcacc accgaaaga agtttcctt 
ccccttatc gtcaaccga gcacctgat cacactcga 
tctctccgg aagcccggg acttgcccg acaattgct 
agtcgatac tgcagctct agccgcctc gcgacaatt 
acaagagct tgatttgtc tttcaccga cctcgagtt 
catccgctc tgacttgct ccacttttt gccccaatt 
gcggatctt ccacattga cggcaaccg aatcaactg 
cacgcagct cgagtggtt ggcggcttg gatgcggcg 
ccaggacga gcccgagta caaaagagt gacatgtgt 
gagcccccc tgagctgtg ggggaccct cctcatcga 
cctccaacc ggtggaagc gaccccaaa ccgccccac 
aaccacaca aacacccac gacacagat ggaggacta 
catcgacat agagtcgtt tctccggga aaacaaatc 
ggaaatttt cccaattac taactccac gcgtggaga 
aatgtccat gtttagtgc tggggcgca ggagaggct 
gcacccggg gctggtcct ggccgctgc catgctagg 
aacagccct agtgctggt tctactggg ggtgaggtt 
tgccaaacg gaggaggga cgcattgag gctcagaca 
ggaggacct cgaggtcag ctcgctgcc tggcgttac 
ggaactatg gtgacattt gagacaagt cgacgaaga 
gagaacaat gctctatga aactaacct aacttaagc 
gattgtaag tgcttgtga taaaagtga attatactt 
ttgtatgac tgagccatt tttttactt gagaattgt 
aagatagcc atattttag acgagattc aaacgggac 
gatttactg tgtctggcg ataattgtg tgatatttt 
aggtgatct ccgaggtgt gttttgcgt ttcttccga 
tctcaatgt tctgattat cggttttgc tacagattg 
tacatacct gtcatgttt atatttcag tgcgacttg 
ttctgtgta ttgtgatag tcccataaa tttaatgaa 
aaaatatag acgtattta aagt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY