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Gene information
General information
TC000701 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 7289177..7298368
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000701
    iBeetle screen iB_07852

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 6 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head split - Penetrance (percentage of animals/eggs on slide): less than 30%

    head capsule size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head appendages shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    legs podomer number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    legs size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000701 RA
      MAQTRIDVL LGVSTVLVL LLPHTVSAK LLESPKFSQ 
      PIPNNTVAV GREAILACV VENLGSYKV AWLRVDTQT 
      ILTIHNHVV TKNHRIGVT HSELKTWYL HIKEVGEND 
      RGWYMCQIN TDPMKSQIC YLDVVVSPD ILDHSTSAD 
      IVVDEGADV SLRCVAKGS PEPSILWKR EDGQLIPSR 
      VGGEVASTS GPTLNISKI KREHMGPYL CIASNGVPP 
      SVSKRIMVV VQFSPSVWI RYQLVGAYD DQQITLECH 
      SEAYPKSIN YWTRDNGEI IPHSVKYVP EIIEDGYKV 
      HMKLTINHL GPLDYGIYK CISKNSLGD MEGTINIYR 
      ISNPALKNS SFHVKSKGS KKVANDITY QIGSTEKDR 
      ADLLYLEDE FYSCAHQLD VSQLLTILW LRVLLYCIT 
      FI
    CDS sequence
    • >TC000701 RA
      atggcccag acacgcatc gatgtgcta ctcggagta 
      tccactgtt ttagtgtta ttgctcccg cacacggtc 
      tcagcgaaa cttttggag agccctaag tttagtcaa 
      cccattcct aataatacc gtcgccgta gggcgtgaa 
      gccatctta gcctgcgtt gttgagaat ctcggctct 
      tataaggta gcgtggcta agagtggac actcaaact 
      attcttacg attcataat cacgtcgtt acaaagaat 
      caccggatt ggagtaaca cacagtgaa ctgaaaacg 
      tggtatctt cacataaaa gaagtggga gagaatgac 
      aggggctgg tatatgtgc cagattaac accgacccg 
      atgaaaagt caaatttgt tacttggac gtcgtagtc 
      tcgcctgat attctggat cattcaacg agcgccgac 
      attgtcgtc gacgaaggg gccgacgta tcgctgcga 
      tgcgtcgct aaagggtcg ccagaaccc tcaattctg 
      tggaaacga gaagacgga caactcata ccgtcgcgt 
      gtggggggc gaggttgcg agtactagt ggccccaca 
      ctcaacata tcaaaaatt aaaagagag cacatggga 
      ccctacctt tgcatcgct tcgaacggc gttcctcct 
      tccgtcagc aaaagaata atggtggtg gtacagttt 
      tctccgtcg gtgtggatt cgctaccaa ttggtaggg 
      gcatatgac gatcaacag atcactctc gaatgccat 
      tccgaagca tatccgaaa tccatcaac tactggact 
      agggataat ggagaaatc attccacac agtgttaaa 
      tatgtgccc gaaattatc gaggatggg tataaagtg 
      catatgaaa ttgaccata aatcatttg ggaccacta 
      gactacgga atctataaa tgcatatca aagaattct 
      ttgggtgat atggaaggc acaataaat atttataga 
      atttctaac ccggcactg aaaaatagc tcgtttcat 
      gtcaagagc aaaggttcg aagaaagta gcgaatgat 
      ataacttat cagataggt tccacagag aaggaccgc 
      gccgacttg ctatatctg gaagatgaa ttttacagt 
      tgcgctcat caacttgat gtctcgcaa ctgcttaca 
      attctatgg ttacgagtg cttctatac tgcatcaca 
      ttcatttaa
    mRNA sequence
    • >TC000701 RA
      gcggcaaaa ttaaacgag cagttaagc aactccagc 
      taaaactaa aagtcgttc agctttaac aactcatag 
      ttttacgag caatgtcgg ggatttttc ggagcgtca 
      tcgagattt tatgtggcc atatagcta aattgtatt 
      aattggggg agcgacgcc aaaaattcg ccgaagaat 
      gcgctcatc taatagcag gaggataat ccgagtctg 
      cggcgcgaa tccaaatga gtgtgtaag ttattcggt 
      aagaaatca aaacataaa gttttgcgc aagcgtccg 
      taatatgta aaggacgag cgtcgggac ggcggcgtc 
      agtcgacat gctgcagga ggccaatgc tgttcccta 
      tggcccaga cacgcatcg atgtgctac tcggagtat 
      ccactgttt tagtgttat tgctcccgc acacggtct 
      cagcgaaac ttttggaga gccctaagt ttagtcaac 
      ccattccta ataataccg tcgccgtag ggcgtgaag 
      ccatcttag cctgcgttg ttgagaatc tcggctctt 
      ataaggtag cgtggctaa gagtggaca ctcaaacta 
      ttcttacga ttcataatc acgtcgtta caaagaatc 
      accggattg gagtaacac acagtgaac tgaaaacgt 
      ggtatcttc acataaaag aagtgggag agaatgaca 
      ggggctggt atatgtgcc agattaaca ccgacccga 
      tgaaaagtc aaatttgtt acttggacg tcgtagtct 
      cgcctgata ttctggatc attcaacga gcgccgaca 
      ttgtcgtcg acgaagggg ccgacgtat cgctgcgat 
      gcgtcgcta aagggtcgc cagaaccct caattctgt 
      ggaaacgag aagacggac aactcatac cgtcgcgtg 
      tggggggcg aggttgcga gtactagtg gccccacac 
      tcaacatat caaaaatta aaagagagc acatgggac 
      cctaccttt gcatcgctt cgaacggcg ttcctcctt 
      ccgtcagca aaagaataa tggtggtgg tacagtttt 
      ctccgtcgg tgtggattc gctaccaat tggtagggg 
      catatgacg atcaacaga tcactctcg aatgccatt 
      ccgaagcat atccgaaat ccatcaact actggacta 
      gggataatg gagaaatca ttccacaca gtgttaaat 
      atgtgcccg aaattatcg aggatgggt ataaagtgc 
      atatgaaat tgaccataa atcatttgg gaccactag 
      actacggaa tctataaat gcatatcaa agaattctt 
      tgggtgata tggaaggca caataaata tttatagaa 
      tttctaacc cggcactga aaaatagct cgtttcatg 
      tcaagagca aaggttcga agaaagtag cgaatgata 
      taacttatc agataggtt ccacagaga aggaccgcg 
      ccgacttgc tatatctgg aagatgaat tttacagtt 
      gcgctcatc aacttgatg tctcgcaac tgcttacaa 
      ttctatggt tacgagtgc ttctatact gcatcacat 
      tcatttaat gtggtgtgt ttcaataag aaagtttca 
      aaaggcgag atgtatgta gcgttgagt tatacagac 
      taacgttaa aaattaact tgctgaaag tttcatcgc 
      attacggac ttagatata agggctatt tttgtacgc 
      ttattttag cataggtgg taataaaat ttttataac 
      aac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY