| General information | |||
| TC032050
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 5910117..5912227 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC032050 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_07841 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC032050 RA
MNFWTLVSL FILAQETLQ SCVISSKLI TIHAWHYRV QTTRTENSI QCVNETISS NLGAKVKPL AEKYSPKWL EIIDTNSPE IPSGVFSLV SALLDLRIN NASVQIIQP GAFNNLTNL TKLSLKCNR LTSVKKTYF ANLSMLEEL DLSYNNIAS VDSKSFVGL TELRMLSLQ NNFLTDLAP DLFKDQIKS LYSLDLSFN KFVTIKNGI VSNLKTLNT IYLNNNNLK ILSGCEFNN TKNLKYVYV NNNNLQALD KLNLPSEVT VFNASHNLI KSMSFWVHA VVYLLDMSS NGITSVQNL SFENATIKE MNLDQNYLG INPSYNHSN FFKSFLVLE TISLGYNNL QQLDRYAFA QTKVLTKLN LTGNYLTKG DFLNFLTKL EYLNLSFNK FDSNLNLTS LANLKELDL GSNNLAQIP FDFFAGLKH LQKLNLGNN KIAKLALGC FRDLQNLRV IDLSSNKIS SLDVGVFAG LLGLNELNL SYNELTQLN GAVFHNLRF LRALGLKKT KVSYTIVEN VLSQSLSLR NVDLAENNW SCSLLTKLI NKFRILKLA DAGDYNVTN IDGVACSYN DDDLADLVN LETRNFATF NAKLSTGNT LLILVLVVL VMHLAIIVL ICKSNRMIK IGQRPKSEQ EMQLVSDC
- >TC032050 RA
atgaacttt tggacgtta gtctcgtta tttatcctg gcacaggaa accctccag tcctgtgtt atctcaagc aaactcata actattcat gcctggcat taccgtgtg caaacaact agaaccgaa aatagcatt caatgtgtc aatgaaacc atttcttcc aacttgggc gcaaaagtg aaaccgctt gctgaaaaa tattcgcca aagtggtta gaaatcatc gacaccaac agtcctgaa ataccttca ggagttttc agcctggtt tcggctctt ttagactta cgaattaac aacgccagt gtccaaata atccaaccc ggggccttc aacaattta acaaatcta acgaaactt tcgctaaag tgcaaccga ctcacaagt gtgaagaaa acttatttt gcaaatttg tcgatgttg gaggaattg gatctaagt tataacaac attgcgagt gtagatagt aaaagtttt gtcggtttg acagaattg cgtatgctt tcgctacaa aataatttc cttactgat ttggcgcct gatctgttc aaggatcag atcaagtcg ttgtatagt ttagatttg tcgtttaac aagttcgta accatcaaa aatgggata gtttcgaat cttaaaacc ttaaacact atttattta aataacaat aacttaaag attttgtca ggatgcgaa tttaacaat acgaagaat ttgaaatat gtttacgtt aataataac aatctgcaa gcgctggat aaattaaat ttgccaagt gaagttaca gttttcaat gcaagccat aatttaatc aaatcgatg agcttttgg gtccatgcc gttgtctat ttgttagac atgagctca aatgggatc acctctgtg caaaattta tcatttgaa aatgcaacg attaaagaa atgaacctg gaccagaac tatttggga attaatcct agctacaac cactcaaac tttttcaaa agtttctta gtactagag actatctct ctcggttat aacaattta caacaattg gatcgttac gcattcgca caaactaaa gttctaacc aaactgaac ctcacagga aattattta accaaaggc gatttttta aattttcta acaaaattg gaatatttg aatctgagc ttcaacaag tttgattcc aacttaaat ttaaccagt ttggcaaac ctgaaagag ttggacctt ggttctaac aacttggct caaattccg tttgacttt ttcgcgggc ttaaagcat ttacagaaa ttaaactta ggtaacaat aaaatagca aaactcgct ttggggtgt ttcagagat ttgcaaaat ctgagagtg attgaccta tcgagtaat aaaatttcg tcgctggat gtgggtgtt tttgcggga ctgcttggt ttgaatgag ttgaattta tcatacaat gaattaact caattgaac ggtgcagtt tttcacaat ttgaggttt ctgcgagcg cttggtttg aaaaaaact aaagttagt tacacgatt gttgaaaat gtgctctcg cagtcactc tcattgagg aacgttgat ctggcagaa aataactgg tcttgttcg cttctcaca aagcttatt aataaattt agaatattg aaacttgca gatgcaggg gattataac gtaactaat atagatgga gttgcttgt agttataac gatgatgat ctagccgat ttagttaac ttggaaacg aggaacttt gcgactttt aatgcaaag ttaagtact gggaatact ttattaatt ttggtgttg gttgttttg gtaatgcac ttggcaatt attgtgcta atttgtaag agcaatagg atgataaag atcgggcag agaccgaaa agtgaacag gaaatgcaa ctcgtgtcc gattgctaa
- >TC032050 RA
gaaaacgga aatgtgata acaattatc aaaaatgtg gacatcaag tccgggacc taaattagt ttgagttgg ttgactgta caccacaca caaactcaa cgattttaa aaatgaact tttggacgt tagtctcgt tatttatcc tggcacagg aaaccctcc agtcctgtg ttatctcaa gcaaactca taactattc atgcctggc attaccgtg tgcaaacaa ctagaaccg aaaatagca ttcaatgtg tcaatgaaa ccatttctt ccaacttgg gcgcaaaag tgaaaccgc ttgctgaaa aatattcgc caaagtggt tagaaatca tcgacacca acagtcctg aaatacctt caggagttt tcagcctgg tttcggctc ttttagact tacgaatta acaacgcca gtgtccaaa taatccaac ccggggcct tcaacaatt taacaaatc taacgaaac tttcgctaa agtgcaacc gactcacaa gtgtgaaga aaacttatt ttgcaaatt tgtcgatgt tggaggaat tggatctaa gttataaca acattgcga gtgtagata gtaaaagtt ttgtcggtt tgacagaat tgcgtatgc tttcgctac aaaataatt tccttactg atttggcgc ctgatctgt tcaaggatc agatcaagt cgttgtata gtttagatt tgtcgttta acaagttcg taaccatca aaaatggga tagtttcga atcttaaaa ccttaaaca ctatttatt taaataaca ataacttaa agattttgt caggatgcg aatttaaca atacgaaga atttgaaat atgtttacg ttaataata acaatctgc aagcgctgg ataaattaa atttgccaa gtgaagtta cagttttca atgcaagcc ataatttaa tcaaatcga tgagctttt gggtccatg ccgttgtct atttgttag acatgagct caaatggga tcacctctg tgcaaaatt tatcatttg aaaatgcaa cgattaaag aaatgaacc tggaccaga actatttgg gaattaatc ctagctaca accactcaa actttttca aaagtttct tagtactag agactatct ctctcggtt ataacaatt tacaacaat tggatcgtt acgcattcg cacaaacta aagttctaa ccaaactga acctcacag gaaattatt taaccaaag gcgattttt taaattttc taacaaaat tggaatatt tgaatctga gcttcaaca agtttgatt ccaacttaa atttaacca gtttggcaa acctgaaag agttggacc ttggttcta acaacttgg ctcaaattc cgtttgact ttttcgcgg gcttaaagc atttacaga aattaaact taggtaaca ataaaatag caaaactcg ctttggggt gtttcagag atttgcaaa atctgagag tgattgacc tatcgagta ataaaattt cgtcgctgg atgtgggtg tttttgcgg gactgcttg gtttgaatg agttgaatt tatcataca atgaattaa ctcaattga acggtgcag tttttcaca atttgaggt ttctgcgag cgcttggtt tgaaaaaaa ctaaagtta gttacacga ttgttgaaa atgtgctct cgcagtcac tctcattga ggaacgttg atctggcag aaaataact ggtcttgtt cgcttctca caaagctta ttaataaat ttagaatat tgaaacttg cagatgcag gggattata acgtaacta atatagatg gagttgctt gtagttata acgatgatg atctagccg atttagtta acttggaaa cgaggaact ttgcgactt ttaatgcaa agttaagta ctgggaata ctttattaa ttttggtgt tggttgttt tggtaatgc acttggcaa ttattgtgc taatttgta agagcaata ggatgataa agatcgggc agagaccga aaagtgaac aggaaatgc aactcgtgt ccgattgct aaacgtaaa tgtaaggaa ataaatatt gtaaagttg t
