Status
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Gene information
General information
TC032050
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC000755
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 5910117..5912227
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032050
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_07841

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032050 RA
      MNFWTLVSL FILAQETLQ SCVISSKLI TIHAWHYRV 
      QTTRTENSI QCVNETISS NLGAKVKPL AEKYSPKWL 
      EIIDTNSPE IPSGVFSLV SALLDLRIN NASVQIIQP 
      GAFNNLTNL TKLSLKCNR LTSVKKTYF ANLSMLEEL 
      DLSYNNIAS VDSKSFVGL TELRMLSLQ NNFLTDLAP 
      DLFKDQIKS LYSLDLSFN KFVTIKNGI VSNLKTLNT 
      IYLNNNNLK ILSGCEFNN TKNLKYVYV NNNNLQALD 
      KLNLPSEVT VFNASHNLI KSMSFWVHA VVYLLDMSS 
      NGITSVQNL SFENATIKE MNLDQNYLG INPSYNHSN 
      FFKSFLVLE TISLGYNNL QQLDRYAFA QTKVLTKLN 
      LTGNYLTKG DFLNFLTKL EYLNLSFNK FDSNLNLTS 
      LANLKELDL GSNNLAQIP FDFFAGLKH LQKLNLGNN 
      KIAKLALGC FRDLQNLRV IDLSSNKIS SLDVGVFAG 
      LLGLNELNL SYNELTQLN GAVFHNLRF LRALGLKKT 
      KVSYTIVEN VLSQSLSLR NVDLAENNW SCSLLTKLI 
      NKFRILKLA DAGDYNVTN IDGVACSYN DDDLADLVN 
      LETRNFATF NAKLSTGNT LLILVLVVL VMHLAIIVL 
      ICKSNRMIK IGQRPKSEQ EMQLVSDC
    CDS sequence
    • >TC032050 RA
      atgaacttt tggacgtta gtctcgtta tttatcctg 
      gcacaggaa accctccag tcctgtgtt atctcaagc 
      aaactcata actattcat gcctggcat taccgtgtg 
      caaacaact agaaccgaa aatagcatt caatgtgtc 
      aatgaaacc atttcttcc aacttgggc gcaaaagtg 
      aaaccgctt gctgaaaaa tattcgcca aagtggtta 
      gaaatcatc gacaccaac agtcctgaa ataccttca 
      ggagttttc agcctggtt tcggctctt ttagactta 
      cgaattaac aacgccagt gtccaaata atccaaccc 
      ggggccttc aacaattta acaaatcta acgaaactt 
      tcgctaaag tgcaaccga ctcacaagt gtgaagaaa 
      acttatttt gcaaatttg tcgatgttg gaggaattg 
      gatctaagt tataacaac attgcgagt gtagatagt 
      aaaagtttt gtcggtttg acagaattg cgtatgctt 
      tcgctacaa aataatttc cttactgat ttggcgcct 
      gatctgttc aaggatcag atcaagtcg ttgtatagt 
      ttagatttg tcgtttaac aagttcgta accatcaaa 
      aatgggata gtttcgaat cttaaaacc ttaaacact 
      atttattta aataacaat aacttaaag attttgtca 
      ggatgcgaa tttaacaat acgaagaat ttgaaatat 
      gtttacgtt aataataac aatctgcaa gcgctggat 
      aaattaaat ttgccaagt gaagttaca gttttcaat 
      gcaagccat aatttaatc aaatcgatg agcttttgg 
      gtccatgcc gttgtctat ttgttagac atgagctca 
      aatgggatc acctctgtg caaaattta tcatttgaa 
      aatgcaacg attaaagaa atgaacctg gaccagaac 
      tatttggga attaatcct agctacaac cactcaaac 
      tttttcaaa agtttctta gtactagag actatctct 
      ctcggttat aacaattta caacaattg gatcgttac 
      gcattcgca caaactaaa gttctaacc aaactgaac 
      ctcacagga aattattta accaaaggc gatttttta 
      aattttcta acaaaattg gaatatttg aatctgagc 
      ttcaacaag tttgattcc aacttaaat ttaaccagt 
      ttggcaaac ctgaaagag ttggacctt ggttctaac 
      aacttggct caaattccg tttgacttt ttcgcgggc 
      ttaaagcat ttacagaaa ttaaactta ggtaacaat 
      aaaatagca aaactcgct ttggggtgt ttcagagat 
      ttgcaaaat ctgagagtg attgaccta tcgagtaat 
      aaaatttcg tcgctggat gtgggtgtt tttgcggga 
      ctgcttggt ttgaatgag ttgaattta tcatacaat 
      gaattaact caattgaac ggtgcagtt tttcacaat 
      ttgaggttt ctgcgagcg cttggtttg aaaaaaact 
      aaagttagt tacacgatt gttgaaaat gtgctctcg 
      cagtcactc tcattgagg aacgttgat ctggcagaa 
      aataactgg tcttgttcg cttctcaca aagcttatt 
      aataaattt agaatattg aaacttgca gatgcaggg 
      gattataac gtaactaat atagatgga gttgcttgt 
      agttataac gatgatgat ctagccgat ttagttaac 
      ttggaaacg aggaacttt gcgactttt aatgcaaag 
      ttaagtact gggaatact ttattaatt ttggtgttg 
      gttgttttg gtaatgcac ttggcaatt attgtgcta 
      atttgtaag agcaatagg atgataaag atcgggcag 
      agaccgaaa agtgaacag gaaatgcaa ctcgtgtcc 
      gattgctaa
    mRNA sequence
    • >TC032050 RA
      gaaaacgga aatgtgata acaattatc aaaaatgtg 
      gacatcaag tccgggacc taaattagt ttgagttgg 
      ttgactgta caccacaca caaactcaa cgattttaa 
      aaatgaact tttggacgt tagtctcgt tatttatcc 
      tggcacagg aaaccctcc agtcctgtg ttatctcaa 
      gcaaactca taactattc atgcctggc attaccgtg 
      tgcaaacaa ctagaaccg aaaatagca ttcaatgtg 
      tcaatgaaa ccatttctt ccaacttgg gcgcaaaag 
      tgaaaccgc ttgctgaaa aatattcgc caaagtggt 
      tagaaatca tcgacacca acagtcctg aaatacctt 
      caggagttt tcagcctgg tttcggctc ttttagact 
      tacgaatta acaacgcca gtgtccaaa taatccaac 
      ccggggcct tcaacaatt taacaaatc taacgaaac 
      tttcgctaa agtgcaacc gactcacaa gtgtgaaga 
      aaacttatt ttgcaaatt tgtcgatgt tggaggaat 
      tggatctaa gttataaca acattgcga gtgtagata 
      gtaaaagtt ttgtcggtt tgacagaat tgcgtatgc 
      tttcgctac aaaataatt tccttactg atttggcgc 
      ctgatctgt tcaaggatc agatcaagt cgttgtata 
      gtttagatt tgtcgttta acaagttcg taaccatca 
      aaaatggga tagtttcga atcttaaaa ccttaaaca 
      ctatttatt taaataaca ataacttaa agattttgt 
      caggatgcg aatttaaca atacgaaga atttgaaat 
      atgtttacg ttaataata acaatctgc aagcgctgg 
      ataaattaa atttgccaa gtgaagtta cagttttca 
      atgcaagcc ataatttaa tcaaatcga tgagctttt 
      gggtccatg ccgttgtct atttgttag acatgagct 
      caaatggga tcacctctg tgcaaaatt tatcatttg 
      aaaatgcaa cgattaaag aaatgaacc tggaccaga 
      actatttgg gaattaatc ctagctaca accactcaa 
      actttttca aaagtttct tagtactag agactatct 
      ctctcggtt ataacaatt tacaacaat tggatcgtt 
      acgcattcg cacaaacta aagttctaa ccaaactga 
      acctcacag gaaattatt taaccaaag gcgattttt 
      taaattttc taacaaaat tggaatatt tgaatctga 
      gcttcaaca agtttgatt ccaacttaa atttaacca 
      gtttggcaa acctgaaag agttggacc ttggttcta 
      acaacttgg ctcaaattc cgtttgact ttttcgcgg 
      gcttaaagc atttacaga aattaaact taggtaaca 
      ataaaatag caaaactcg ctttggggt gtttcagag 
      atttgcaaa atctgagag tgattgacc tatcgagta 
      ataaaattt cgtcgctgg atgtgggtg tttttgcgg 
      gactgcttg gtttgaatg agttgaatt tatcataca 
      atgaattaa ctcaattga acggtgcag tttttcaca 
      atttgaggt ttctgcgag cgcttggtt tgaaaaaaa 
      ctaaagtta gttacacga ttgttgaaa atgtgctct 
      cgcagtcac tctcattga ggaacgttg atctggcag 
      aaaataact ggtcttgtt cgcttctca caaagctta 
      ttaataaat ttagaatat tgaaacttg cagatgcag 
      gggattata acgtaacta atatagatg gagttgctt 
      gtagttata acgatgatg atctagccg atttagtta 
      acttggaaa cgaggaact ttgcgactt ttaatgcaa 
      agttaagta ctgggaata ctttattaa ttttggtgt 
      tggttgttt tggtaatgc acttggcaa ttattgtgc 
      taatttgta agagcaata ggatgataa agatcgggc 
      agagaccga aaagtgaac aggaaatgc aactcgtgt 
      ccgattgct aaacgtaaa tgtaaggaa ataaatatt 
      gtaaagttg t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY