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Gene information
General information
TC032050
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC000755 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 5910117..5912227
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC032050
    iBeetle screen iB_07841

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032050 RA
      MNFWTLVSL FILAQETLQ SCVISSKLI TIHAWHYRV 
QTTRTENSI QCVNETISS NLGAKVKPL AEKYSPKWL 
EIIDTNSPE IPSGVFSLV SALLDLRIN NASVQIIQP 
GAFNNLTNL TKLSLKCNR LTSVKKTYF ANLSMLEEL 
DLSYNNIAS VDSKSFVGL TELRMLSLQ NNFLTDLAP 
DLFKDQIKS LYSLDLSFN KFVTIKNGI VSNLKTLNT 
IYLNNNNLK ILSGCEFNN TKNLKYVYV NNNNLQALD 
KLNLPSEVT VFNASHNLI KSMSFWVHA VVYLLDMSS 
NGITSVQNL SFENATIKE MNLDQNYLG INPSYNHSN 
FFKSFLVLE TISLGYNNL QQLDRYAFA QTKVLTKLN 
LTGNYLTKG DFLNFLTKL EYLNLSFNK FDSNLNLTS 
LANLKELDL GSNNLAQIP FDFFAGLKH LQKLNLGNN 
KIAKLALGC FRDLQNLRV IDLSSNKIS SLDVGVFAG 
LLGLNELNL SYNELTQLN GAVFHNLRF LRALGLKKT 
KVSYTIVEN VLSQSLSLR NVDLAENNW SCSLLTKLI 
NKFRILKLA DAGDYNVTN IDGVACSYN DDDLADLVN 
LETRNFATF NAKLSTGNT LLILVLVVL VMHLAIIVL 
ICKSNRMIK IGQRPKSEQ EMQLVSDC
    CDS sequence
    • >TC032050 RA
      atgaacttt tggacgtta gtctcgtta tttatcctg 
gcacaggaa accctccag tcctgtgtt atctcaagc 
aaactcata actattcat gcctggcat taccgtgtg 
caaacaact agaaccgaa aatagcatt caatgtgtc 
aatgaaacc atttcttcc aacttgggc gcaaaagtg 
aaaccgctt gctgaaaaa tattcgcca aagtggtta 
gaaatcatc gacaccaac agtcctgaa ataccttca 
ggagttttc agcctggtt tcggctctt ttagactta 
cgaattaac aacgccagt gtccaaata atccaaccc 
ggggccttc aacaattta acaaatcta acgaaactt 
tcgctaaag tgcaaccga ctcacaagt gtgaagaaa 
acttatttt gcaaatttg tcgatgttg gaggaattg 
gatctaagt tataacaac attgcgagt gtagatagt 
aaaagtttt gtcggtttg acagaattg cgtatgctt 
tcgctacaa aataatttc cttactgat ttggcgcct 
gatctgttc aaggatcag atcaagtcg ttgtatagt 
ttagatttg tcgtttaac aagttcgta accatcaaa 
aatgggata gtttcgaat cttaaaacc ttaaacact 
atttattta aataacaat aacttaaag attttgtca 
ggatgcgaa tttaacaat acgaagaat ttgaaatat 
gtttacgtt aataataac aatctgcaa gcgctggat 
aaattaaat ttgccaagt gaagttaca gttttcaat 
gcaagccat aatttaatc aaatcgatg agcttttgg 
gtccatgcc gttgtctat ttgttagac atgagctca 
aatgggatc acctctgtg caaaattta tcatttgaa 
aatgcaacg attaaagaa atgaacctg gaccagaac 
tatttggga attaatcct agctacaac cactcaaac 
tttttcaaa agtttctta gtactagag actatctct 
ctcggttat aacaattta caacaattg gatcgttac 
gcattcgca caaactaaa gttctaacc aaactgaac 
ctcacagga aattattta accaaaggc gatttttta 
aattttcta acaaaattg gaatatttg aatctgagc 
ttcaacaag tttgattcc aacttaaat ttaaccagt 
ttggcaaac ctgaaagag ttggacctt ggttctaac 
aacttggct caaattccg tttgacttt ttcgcgggc 
ttaaagcat ttacagaaa ttaaactta ggtaacaat 
aaaatagca aaactcgct ttggggtgt ttcagagat 
ttgcaaaat ctgagagtg attgaccta tcgagtaat 
aaaatttcg tcgctggat gtgggtgtt tttgcggga 
ctgcttggt ttgaatgag ttgaattta tcatacaat 
gaattaact caattgaac ggtgcagtt tttcacaat 
ttgaggttt ctgcgagcg cttggtttg aaaaaaact 
aaagttagt tacacgatt gttgaaaat gtgctctcg 
cagtcactc tcattgagg aacgttgat ctggcagaa 
aataactgg tcttgttcg cttctcaca aagcttatt 
aataaattt agaatattg aaacttgca gatgcaggg 
gattataac gtaactaat atagatgga gttgcttgt 
agttataac gatgatgat ctagccgat ttagttaac 
ttggaaacg aggaacttt gcgactttt aatgcaaag 
ttaagtact gggaatact ttattaatt ttggtgttg 
gttgttttg gtaatgcac ttggcaatt attgtgcta 
atttgtaag agcaatagg atgataaag atcgggcag 
agaccgaaa agtgaacag gaaatgcaa ctcgtgtcc 
gattgctaa
    mRNA sequence
    • >TC032050 RA
      gaaaacgga aatgtgata acaattatc aaaaatgtg 
gacatcaag tccgggacc taaattagt ttgagttgg 
ttgactgta caccacaca caaactcaa cgattttaa 
aaatgaact tttggacgt tagtctcgt tatttatcc 
tggcacagg aaaccctcc agtcctgtg ttatctcaa 
gcaaactca taactattc atgcctggc attaccgtg 
tgcaaacaa ctagaaccg aaaatagca ttcaatgtg 
tcaatgaaa ccatttctt ccaacttgg gcgcaaaag 
tgaaaccgc ttgctgaaa aatattcgc caaagtggt 
tagaaatca tcgacacca acagtcctg aaatacctt 
caggagttt tcagcctgg tttcggctc ttttagact 
tacgaatta acaacgcca gtgtccaaa taatccaac 
ccggggcct tcaacaatt taacaaatc taacgaaac 
tttcgctaa agtgcaacc gactcacaa gtgtgaaga 
aaacttatt ttgcaaatt tgtcgatgt tggaggaat 
tggatctaa gttataaca acattgcga gtgtagata 
gtaaaagtt ttgtcggtt tgacagaat tgcgtatgc 
tttcgctac aaaataatt tccttactg atttggcgc 
ctgatctgt tcaaggatc agatcaagt cgttgtata 
gtttagatt tgtcgttta acaagttcg taaccatca 
aaaatggga tagtttcga atcttaaaa ccttaaaca 
ctatttatt taaataaca ataacttaa agattttgt 
caggatgcg aatttaaca atacgaaga atttgaaat 
atgtttacg ttaataata acaatctgc aagcgctgg 
ataaattaa atttgccaa gtgaagtta cagttttca 
atgcaagcc ataatttaa tcaaatcga tgagctttt 
gggtccatg ccgttgtct atttgttag acatgagct 
caaatggga tcacctctg tgcaaaatt tatcatttg 
aaaatgcaa cgattaaag aaatgaacc tggaccaga 
actatttgg gaattaatc ctagctaca accactcaa 
actttttca aaagtttct tagtactag agactatct 
ctctcggtt ataacaatt tacaacaat tggatcgtt 
acgcattcg cacaaacta aagttctaa ccaaactga 
acctcacag gaaattatt taaccaaag gcgattttt 
taaattttc taacaaaat tggaatatt tgaatctga 
gcttcaaca agtttgatt ccaacttaa atttaacca 
gtttggcaa acctgaaag agttggacc ttggttcta 
acaacttgg ctcaaattc cgtttgact ttttcgcgg 
gcttaaagc atttacaga aattaaact taggtaaca 
ataaaatag caaaactcg ctttggggt gtttcagag 
atttgcaaa atctgagag tgattgacc tatcgagta 
ataaaattt cgtcgctgg atgtgggtg tttttgcgg 
gactgcttg gtttgaatg agttgaatt tatcataca 
atgaattaa ctcaattga acggtgcag tttttcaca 
atttgaggt ttctgcgag cgcttggtt tgaaaaaaa 
ctaaagtta gttacacga ttgttgaaa atgtgctct 
cgcagtcac tctcattga ggaacgttg atctggcag 
aaaataact ggtcttgtt cgcttctca caaagctta 
ttaataaat ttagaatat tgaaacttg cagatgcag 
gggattata acgtaacta atatagatg gagttgctt 
gtagttata acgatgatg atctagccg atttagtta 
acttggaaa cgaggaact ttgcgactt ttaatgcaa 
agttaagta ctgggaata ctttattaa ttttggtgt 
tggttgttt tggtaatgc acttggcaa ttattgtgc 
taatttgta agagcaata ggatgataa agatcgggc 
agagaccga aaagtgaac aggaaatgc aactcgtgt 
ccgattgct aaacgtaaa tgtaaggaa ataaatatt 
gtaaagttg t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY