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Gene information
General information
TC032037
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC000769
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 5708643..5712146
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032037
    iBeetle screen iB_07838

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032037 RA
      MGSQQESEV KNIITGLLT SNPLRCTIQ KLCKDFYET 
      VGYNVPFRK LGFTNVEDY LHSIPDTVQ VFGHGPSAE 
      VQGVFKPKS AHINLMVVK QKNVPKTNR KQIVPKRKS 
      LPRVDDGYR SRVPPGIQN NLRKLILRF PDGIWCTKL 
      PEVYKKMFN VDLNYQIFH YRSLIEMCT DLTEIFHYT 
      QISSDDYML YDRNSVAPS LNQNDTVLQ LSDKDNLAK 
      AADWSLGSA LIPSNIVPL SEEIPRYFP NTTTQGEYL 
      EVTIGEIYD INNFWVYLH DGQLKKLMT DLQEHFQSQ 
      RNRYVISEL FLQKGLYCV VLYSDFYHR GVIMDLWDT 
      EPPSVKVLF IDYGSIGEI PCHHIWYLP KEFSKVPCQ 
      AIKASLYNI KSELDKNQC TRKFEHLIS NKQLIAEVL 
      KIDKKACVR LYLKLLVTF EL
    CDS sequence
    • >TC032037 RA
      atggggtcg cagcaagaa tcagaagtc aaaaacata 
      attacgggg ttactgact tcgaacccg ttacgatgt 
      actattcaa aaactatgc aaagatttc tacgaaact 
      gtgggatac aatgttccg ttcaggaaa cttggtttc 
      acaaatgtt gaggattat ttacattca attccagat 
      acggttcaa gtttttggg catggacct tctgccgaa 
      gttcaaggc gtttttaag cctaagtca gcccatata 
      aacctaatg gtggtgaaa caaaaaaat gttcccaaa 
      actaacaga aagcaaata gttcccaaa agaaagtct 
      cttcctcga gttgatgac ggatacaga tctagagta 
      ccacccggt atacaaaac aacttaaga aaactcatt 
      ttacgattt cccgatggt atttggtgt acaaagtta 
      ccagaagtg tataaaaaa atgtttaac gtggacctt 
      aattaccaa atatttcac tacaggtct ttaattgaa 
      atgtgcacc gatttaacg gaaattttt cactatact 
      caaatttcc tcagatgat tatatgcta tatgatagg 
      aattcggta gcaccttca ttgaatcaa aatgacaca 
      gttttgcag ttatcagac aaagacaac ttagcaaaa 
      gcggcagat tggtcactt ggctcagct ttaattcct 
      tcaaatatt gtgccttta tcggaggaa atccctcgt 
      tattttccc aacactaca acacaaggc gaatatctc 
      gaagttacg ataggagaa atatatgac attaataat 
      ttttgggta tatttacat gacggtcag ctaaagaaa 
      ctcatgact gatttacag gaacatttt caaagtcaa 
      cgaaatcgc tacgttatc tctgaatta tttttacag 
      aaaggattg tattgtgtt gttttatat agtgatttt 
      taccacaga ggtgttatt atggatttg tgggacacg 
      gagccaccg tctgtcaaa gtgcttttt attgattat 
      ggtagtatc ggggaaata ccttgtcat cacatttgg 
      taccttccc aaggagttc agtaaagta ccatgtcag 
      gcaataaaa gcaagtctg tacaacata aaatcagaa 
      ttggacaaa aatcaatgt actagaaaa tttgagcat 
      cttattagt aacaaacaa ttaatagcc gaagtttta 
      aaaattgat aaaaaggct tgtgtgaga ttatattta 
      aaactttta gtaacattt gaattgtag
    mRNA sequence
    • >TC032037 RA
      catataacc tttcttttt gtggtagaa aagaaattt 
      ttaattcaa atttttgtg cttagtttt ataggaaca 
      ataagtttt gtatcatgt ttacgatta gtttagctt 
      aaaatgggg tcgcagcaa gaatcagaa gtcaaaaac 
      ataattacg gggttactg acttcgaac ccgttacga 
      tgtactatt caaaaacta tgcaaagat ttctacgaa 
      actgtggga tacaatgtt ccgttcagg aaacttggt 
      ttcacaaat gttgaggat tatttacat tcaattcca 
      gatacggtt caagttttt gggcatgga ccttctgcc 
      gaagttcaa ggcgttttt aagcctaag tcagcccat 
      ataaaccta atggtggtg aaacaaaaa aatgttccc 
      aaaactaac agaaagcaa atagttccc aaaagaaag 
      tctcttcct cgagttgat gacggatac agatctaga 
      gtaccaccc ggtatacaa aacaactta agaaaactc 
      attttacga tttcccgat ggtatttgg tgtacaaag 
      ttaccagaa gtgtataaa aaaatgttt aacgtggac 
      cttaattac caaatattt cactacagg tctttaatt 
      gaaatgtgc accgattta acggaaatt tttcactat 
      actcaaatt tcctcagat gattatatg ctatatgat 
      aggaattcg gtagcacct tcattgaat caaaatgac 
      acagttttg cagttatca gacaaagac aacttagca 
      aaagcggca gattggtca cttggctca gctttaatt 
      ccttcaaat attgtgcct ttatcggag gaaatccct 
      cgttatttt cccaacact acaacacaa ggcgaatat 
      ctcgaagtt acgatagga gaaatatat gacattaat 
      aatttttgg gtatattta catgacggt cagctaaag 
      aaactcatg actgattta caggaacat tttcaaagt 
      caacgaaat cgctacgtt atctctgaa ttattttta 
      cagaaagga ttgtattgt gttgtttta tatagtgat 
      ttttaccac agaggtgtt attatggat ttgtgggac 
      acggagcca ccgtctgtc aaagtgctt tttattgat 
      tatggtagt atcggggaa ataccttgt catcacatt 
      tggtacctt cccaaggag ttcagtaaa gtaccatgt 
      caggcaata aaagcaagt ctgtacaac ataaaatca 
      gaattggac aaaaatcaa tgtactaga aaatttgag 
      catcttatt agtaacaaa caattaata gccgaagtt 
      ttaaaaatt gataaaaag gcttgtgtg agattatat 
      ttaaaactt ttagtaaca tttgaattg taggagttg 
      cttttggaa atttatttg gctgataca tcagatgaa 
      gaaaacata ttttacatt aatgatcga cttgttgac 
      gagggatat gcgagttat gtcacatga cccgtttgc 
      gtttcgatt ttttacgag ttgtactat tctgtttga 
      aaataaaag tattatttt att
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY