Status
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Gene information
General information
TC004975 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 5404747..5407697
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004975
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_07833

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004975 RA
      MSAKKVKRS SNKPKTRKE IRKEQRKSK KSRRHEYYT 
      NRKKPGQFV RINPQMAAE IYGEEATKP AKESNQGKQ 
      KNQVKTKME AENVRDKEK KQQSKLQKD MSKQRNKQL 
      VEANLEEDR TIRRLEKQL KLNKRKSKS VPASFSRDG 
      LDYLLEVCD PENLKNTAL AEQQLAEVN DEFEEDLVL 
      MTEDKPSKI RKQTDDYLN SSEDEIEEE ENFSESETE 
      EIPDMKKRK IDEINDDKS EDSESFEEE ENSDEESPE 
      KTNDDGTWE DIYGRLRAK DGSVVTSQK YVPPGARTL 
      LENGPEDKM RSQKLERLK RQLKGLINR LAESNMHSI 
      ATQIEELYM SNSRNDMNN TITNLVTEA LVSNILAPE 
      RLLMEHILL ITILHANVG TEVGAHFLQ TIVKMFDDY 
      LKKSNVEDK KLDNVVVIL AQLYNFKLF DCKLIYEIL 
      DTLAENFEE KHVECILHV LRNVGFNLR KDNPIALKN 
      LILSLQKRA SNASNELKD NSRVKFMLD ILLAIKNNN 
      VSKIPNYDT TYSEHLKKI MKGFIRKGN YVTQLNISL 
      EDLLKAEEK GKWWVVGSA WVGNTDKTP ENVKETPNS 
      FSQKLLELA TKQRMNTDA RKNIFCVIM SAEDYLDAF 
      EKLLRLSLK NQQEREIIH VILHCCLQE KAYNPYYAT 
      LAQKFCEYD RRFQMTIKY SIWDKLKAL KDHSATQIS 
      HLAKLLSHL FIEKGLPIS TLKVVQFSE LDKVTLRFV 
      RQILLGILL HEDLDVCKG VFEKVGFSD KLKMFRESL 
      RLFLHHFLL KNLESHNVE EEKKELLQQ RVKIVEKLL 
      VGREIRL
    CDS sequence
    • >TC004975 RA
      atgtcagct aagaaggta aaaaggtcg tcaaataaa 
      ccaaaaacg aggaaagaa atccgcaaa gaacaaaga 
      aaaagtaaa aaaagtcga cgtcatgag tattataca 
      aataggaaa aaaccgggc caatttgtg cgaattaat 
      ccacaaatg gctgcagaa atatacggt gaggaagct 
      acaaaacca gcaaaagaa tctaatcaa ggaaaacag 
      aaaaatcaa gtaaaaaca aagatggag gccgaaaat 
      gtccgagat aaggagaaa aaacaacaa agtaaatta 
      caaaaagac atgagcaaa caaagaaat aaacaatta 
      gtggaagct aatttagag gaagaccgc acaatacga 
      cgattggaa aaacagttg aagttgaac aaaagaaaa 
      tcaaaatcg gtgcctgct tcgttcagc agagacggc 
      ttggactat ttgttggaa gtttgtgat ccagaaaat 
      ttgaaaaat acggcactt gctgaacaa caattagct 
      gaagtaaat gatgagttt gaggaagat ttggtttta 
      atgacagaa gataaacct tctaaaatt cggaaacaa 
      accgacgat tatctaaac tcatctgaa gatgaaatt 
      gaagaagag gaaaatttt agtgaaagt gaaactgaa 
      gaaattcca gatatgaag aagcgaaaa attgatgaa 
      attaatgac gataaaagt gaagattca gaaagtttt 
      gaagaagag gaaaactca gacgaggaa tcccctgaa 
      aaaacaaat gacgatgga acgtgggaa gatatttac 
      ggccgtttg agggccaaa gatggctct gttgtaacg 
      agtcaaaaa tacgttcca cctggtgca agaacgttg 
      ctagaaaat ggaccagaa gataaaatg aggagccaa 
      aaactggag agattaaaa aggcaactc aagggtttg 
      ataaatcga ttggctgag agtaacatg cacagtatt 
      gccacacaa attgaagag ctgtatatg agcaatagc 
      agaaacgac atgaataat acaatcact aatttagtg 
      actgaagct ttagtgtca aatattcta gcaccagag 
      agactttta atggaacac attttacta atcacgatt 
      ttacacgcg aatgttggg acggaagtt ggtgctcat 
      tttttgcaa acaatcgta aagatgttt gacgattat 
      ttgaaaaag tcaaatgtt gaagataaa aaactggac 
      aatgttgtt gtaattttg gcacaactc tacaatttc 
      aaattgttt gattgcaaa ttaatttac gagattctt 
      gatactttg gctgaaaat tttgaggaa aaacacgtc 
      gagtgcatt ttacatgtt ttaagaaat gtcggtttt 
      aatttgcga aaagataat ccaattgcg cttaaaaat 
      ctaattttg agtctccaa aaacgggcg tctaatgcc 
      tccaatgag ctaaaggat aattctaga gtaaagttc 
      atgttggat attctacta gcaataaaa aataacaac 
      gtttcaaaa atcccaaat tatgacaca acttactcc 
      gaacatttg aaaaaaatc atgaaaggt tttatccga 
      aaaggcaat tatgtcacc caacttaac atttctctg 
      gaagattta cttaaagct gaggaaaag ggcaaatgg 
      tgggttgtg ggttcggca tgggttggc aacactgat 
      aaaacccca gaaaatgtt aaggaaact ccaaattca 
      ttttcacaa aaactatta gaattggca acaaaacag 
      agaatgaat acagacgct cgcaaaaat attttctgt 
      gttattatg tcagcggag gattattta gacgcgttt 
      gaaaaactc ctccgtctt tcgttgaaa aatcagcaa 
      gaacgcgaa attatccac gtgatttta cactgttgc 
      ttgcaggaa aaagcgtac aatccgtat tacgcgact 
      ttggcgcaa aaattctgc gagtatgac cggagattt 
      cagatgacg ataaaatat tcaatttgg gacaaacta 
      aaggccctt aaggaccat tccgcgacc caaatctca 
      cacttggcc aagcttctg agtcatctg ttcatagaa 
      aaaggtctc ccgatatcg acattgaag gtcgtccag 
      ttcagtgaa ttggataaa gtcactttg cgatttgtt 
      agacagatt ctgttaggg attttatta catgaggat 
      ttggatgtt tgtaaagga gtttttgag aaagtggga 
      ttttctgac aagttgaaa atgttcagg gagagttta 
      agactgttt ctgcaccat tttttgttg aaaaatctt 
      gaatcgcat aatgtcgaa gaggagaag aaagaattg 
      ttacaacag agggtcaaa atcgtggaa aaactctta 
      gttgggaga gaaatcaga ttgtaa
    mRNA sequence
    • >TC004975 RA
      gttgtcaaa gattccata accttgctt ttgcacgtt 
      ttgtatatt tttcttact tttcacgaa tgtgaccaa 
      ttttccgct taattttta ctatgtcag ctaagaagg 
      taaaaaggt cgtcaaata aaccaaaaa cgaggaaag 
      aaatccgca aagaacaaa gaaaaagta aaaaaagtc 
      gacgtcatg agtattata caaatagga aaaaaccgg 
      gccaatttg tgcgaatta atccacaaa tggctgcag 
      aaatatacg gtgaggaag ctacaaaac cagcaaaag 
      aatctaatc aaggaaaac agaaaaatc aagtaaaaa 
      caaagatgg aggccgaaa atgtccgag ataaggaga 
      aaaaacaac aaagtaaat tacaaaaag acatgagca 
      aacaaagaa ataaacaat tagtggaag ctaatttag 
      aggaagacc gcacaatac gacgattgg aaaaacagt 
      tgaagttga acaaaagaa aatcaaaat cggtgcctg 
      cttcgttca gcagagacg gcttggact atttgttgg 
      aagtttgtg atccagaaa atttgaaaa atacggcac 
      ttgctgaac aacaattag ctgaagtaa atgatgagt 
      ttgaggaag atttggttt taatgacag aagataaac 
      cttctaaaa ttcggaaac aaaccgacg attatctaa 
      actcatctg aagatgaaa ttgaagaag aggaaaatt 
      ttagtgaaa gtgaaactg aagaaattc cagatatga 
      agaagcgaa aaattgatg aaattaatg acgataaaa 
      gtgaagatt cagaaagtt ttgaagaag aggaaaact 
      cagacgagg aatcccctg aaaaaacaa atgacgatg 
      gaacgtggg aagatattt acggccgtt tgagggcca 
      aagatggct ctgttgtaa cgagtcaaa aatacgttc 
      cacctggtg caagaacgt tgctagaaa atggaccag 
      aagataaaa tgaggagcc aaaaactgg agagattaa 
      aaaggcaac tcaagggtt tgataaatc gattggctg 
      agagtaaca tgcacagta ttgccacac aaattgaag 
      agctgtata tgagcaata gcagaaacg acatgaata 
      atacaatca ctaatttag tgactgaag ctttagtgt 
      caaatattc tagcaccag agagacttt taatggaac 
      acattttac taatcacga ttttacacg cgaatgttg 
      ggacggaag ttggtgctc attttttgc aaacaatcg 
      taaagatgt ttgacgatt atttgaaaa agtcaaatg 
      ttgaagata aaaaactgg acaatgttg ttgtaattt 
      tggcacaac tctacaatt tcaaattgt ttgattgca 
      aattaattt acgagattc ttgatactt tggctgaaa 
      attttgagg aaaaacacg tcgagtgca ttttacatg 
      ttttaagaa atgtcggtt ttaatttgc gaaaagata 
      atccaattg cgcttaaaa atctaattt tgagtctcc 
      aaaaacggg cgtctaatg cctccaatg agctaaagg 
      ataattcta gagtaaagt tcatgttgg atattctac 
      tagcaataa aaaataaca acgtttcaa aaatcccaa 
      attatgaca caacttact ccgaacatt tgaaaaaaa 
      tcatgaaag gttttatcc gaaaaggca attatgtca 
      cccaactta acatttctc tggaagatt tacttaaag 
      ctgaggaaa agggcaaat ggtgggttg tgggttcgg 
      catgggttg gcaacactg ataaaaccc cagaaaatg 
      ttaaggaaa ctccaaatt cattttcac aaaaactat 
      tagaattgg caacaaaac agagaatga atacagacg 
      ctcgcaaaa atattttct gtgttatta tgtcagcgg 
      aggattatt tagacgcgt ttgaaaaac tcctccgtc 
      tttcgttga aaaatcagc aagaacgcg aaattatcc 
      acgtgattt tacactgtt gcttgcagg aaaaagcgt 
      acaatccgt attacgcga ctttggcgc aaaaattct 
      gcgagtatg accggagat ttcagatga cgataaaat 
      attcaattt gggacaaac taaaggccc ttaaggacc 
      attccgcga cccaaatct cacacttgg ccaagcttc 
      tgagtcatc tgttcatag aaaaaggtc tcccgatat 
      cgacattga aggtcgtcc agttcagtg aattggata 
      aagtcactt tgcgatttg ttagacaga ttctgttag 
      ggattttat tacatgagg atttggatg tttgtaaag 
      gagtttttg agaaagtgg gattttctg acaagttga 
      aaatgttca gggagagtt taagactgt ttctgcacc 
      attttttgt tgaaaaatc ttgaatcgc ataatgtcg 
      aagaggaga agaaagaat tgttacaac agagggtca 
      aaatcgtgg aaaaactct tagttggga gagaaatca 
      gattgtaat tactgataa acacacgtg aaataaaat 
      attttttac aat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY