| General information | |||
| TC032020
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 4660690..4685834 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC032020 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_07827 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC032020 RA
MLAKFWIQC LCSLTVFVQ CSWTAATVS QSTIHDQEY WYEQARIAL RKRLQYATD RRPHAKNVV LFVGDGMGV ATATAARIL RGQRLGKRG EDHELAWDT FPAVAFAKT YNMDAQIGE SSACATALM CGVKTNFET VGLDARGRF ENCFSSFSS RVSSLIDWA QESGKSTGI VTNTRITHA TPAALYGHS PSRYWEDDS KVPPASRKS CKDLARQLI ENDPGRNIN VLLGGGRRH WLPKVAHDP EQTKEEGRR LDGRNLIDD WVRDKKKRG LKAEYVWNK GQLDKINPN QVDYLLGLF AYSHMDFEA DRDAGPSGD PSLADMTRS ALSILLKNP KGFFLFVEG GRIDHAHHY NNAYRALDE TLAMETALL AALALINPT ETLIVVTSD HSHVLTMGG QATPRGHPI LGPDSKVSD IDGQPYTTI LYGNGPGYA TPRIVPMNT TTASEDRNQ VHGSAVPRQ WATHGGEDV PVYALGPLA TTLFTGTFD QSYIPHAIA YSACLAEHR LRCQGIDNY TAPQVTTCV APEVSSVSA AGAPVVIAS SVMADDGTR VKSGVGHMG ATQGVLLAV MLEFAVT
- >TC032020 RA
atgttggcc aagttttgg atacagtgt ttgtgcagt ttgacagta tttgtgcag tgttcttgg acggctgcc acagttagt caaagtacc attcatgac caggaatat tggtacgaa caagcacga atcgctttg cggaagaga ctgcaatat gctaccgac cgccggcca catgccaag aatgtggtg ttgtttgtg ggtgatggg atgggcgta gccacggcc actgccgcc aggatcctg agaggacaa cggctggga aaaagggga gaggaccac gagctcgcc tgggacacc ttcccggcc gtggccttc gctaagact tacaacatg gacgcccag ataggagag tcatcagca tgcgctacg gcgttgatg tgcggcgtt aaaacaaac tttgaaacg gtcggtttg gatgctcga ggtcgtttc gaaaactgt ttttctagc ttctcttca cgagtctca tcgctgata gattgggca caagagtca gggaaatcg acaggaatt gtgacaaac acccgaata actcacgcc acacccgca gctctgtat ggtcattct ccgtcacgc tattgggag gacgattcg aaagttccg ccagcgtct cgaaaatcc tgcaaggat ttagctcgg caactcatc gaaaatgat cctggacgg aacatcaac gttttgttg ggcggcgga aggcgacac tggttgcca aaagttgcc cacgatcca gaacaaact aaagaggaa ggtcggcgc ctcgatgga cgtaatctc atcgatgac tgggttcga gataagaaa aagcgtgga ctcaaagct gaatacgtc tggaataaa ggacagctc gataaaatt aaccccaat caagtcgac tacttgctc ggtttattc gcttattcg cacatggat tttgaagcc gatcgtgac gctggccct tctggggat ccctccctg gctgatatg acgcgttca gccctttcc atcctgttg aaaaatccg aaaggcttc ttcttgttc gtcgaagga ggacggatc gatcacgcc caccattac aacaacgct tacagggct ttagacgaa acgctggcg atggagacg gcccttctg gcggctctg gccctcata aacccaaca gagacgtta attgttgtt acttcggat cattcgcac gtcctcacg atggggggc caagccacg ccgagagga cacccgatc ctaggtcct gacagcaaa gtatccgac atcgacggc caaccctac accaccatc ctctacggc aacggcccc ggctacgcc acccctcgc atcgtgccc atgaacacc accaccgct tccgaagac cgcaaccag gtgcacggc tcggctgtg ccccggcaa tgggccacc cacggcggc gaggacgtg cccgtctat gcactaggt cccctggcc accaccctc ttcaccggg accttcgac cagagctac atcccgcac gccatcgct tacagcgcc tgtttggcc gaacatcgc ctgcgctgc cagggaatc gacaattac accgccccc caggtgacc acgtgcgtc gctccggaa gtgtcgagc gtttccgct gccggagct ccggtggtt atcgccagc agcgtgatg gccgatgat gggacgagg gttaagtcg ggggtgggg cacatgggg gccacccag ggggtgctc ctggcagtg atgctggag tttgctgtt acgtaa
- >TC032020 RA
tcgaaagtg gaattttgt acaaaacac gcacgtagg aaggattta gtgaactaa tcccgcgac atcgtccca ctgcaccga atttggata gacaatgaa tggttgtca ttagttatc cccagaaga taacactaa tcaaatttt gggaggacc tgtttgcaa ccactagtg ttagatcag tgatgatca atttagtgt tctagtcgg agagttttt tgggacaat gttggccaa gttttggat acagtgttt gtgcagttt gacagtatt tgtgcagtg ttcttggac ggctgccac agttagtca aagtaccat tcatgacca ggaatattg gtacgaaca agcacgaat cgctttgcg gaagagact gcaatatgc taccgaccg ccggccaca tgccaagaa tgtggtgtt gtttgtggg tgatgggat gggcgtagc cacggccac tgccgccag gatcctgag aggacaacg gctgggaaa aaggggaga ggaccacga gctcgcctg ggacacctt cccggccgt ggccttcgc taagactta caacatgga cgcccagat aggagagtc atcagcatg cgctacggc gttgatgtg cggcgttaa aacaaactt tgaaacggt cggtttgga tgctcgagg tcgtttcga aaactgttt ttctagctt ctcttcacg agtctcatc gctgataga ttgggcaca agagtcagg gaaatcgac aggaattgt gacaaacac ccgaataac tcacgccac acccgcagc tctgtatgg tcattctcc gtcacgcta ttgggagga cgattcgaa agttccgcc agcgtctcg aaaatcctg caaggattt agctcggca actcatcga aaatgatcc tggacggaa catcaacgt tttgttggg cggcggaag gcgacactg gttgccaaa agttgccca cgatccaga acaaactaa agaggaagg tcggcgcct cgatggacg taatctcat cgatgactg ggttcgaga taagaaaaa gcgtggact caaagctga atacgtctg gaataaagg acagctcga taaaattaa ccccaatca agtcgacta cttgctcgg tttattcgc ttattcgca catggattt tgaagccga tcgtgacgc tggcccttc tggggatcc ctccctggc tgatatgac gcgttcagc cctttccat cctgttgaa aaatccgaa aggcttctt cttgttcgt cgaaggagg acggatcga tcacgccca ccattacaa caacgctta cagggcttt agacgaaac gctggcgat ggagacggc ccttctggc ggctctggc cctcataaa cccaacaga gacgttaat tgttgttac ttcggatca ttcgcacgt cctcacgat ggggggcca agccacgcc gagaggaca cccgatcct aggtcctga cagcaaagt atccgacat cgacggcca accctacac caccatcct ctacggcaa cggccccgg ctacgccac ccctcgcat cgtgcccat gaacaccac caccgcttc cgaagaccg caaccaggt gcacggctc ggctgtgcc ccggcaatg ggccaccca cggcggcga ggacgtgcc cgtctatgc actaggtcc cctggccac caccctctt caccgggac cttcgacca gagctacat cccgcacgc catcgctta cagcgcctg tttggccga acatcgcct gcgctgcca gggaatcga caattacac cgcccccca ggtgaccac gtgcgtcgc tccggaagt gtcgagcgt ttccgctgc cggagctcc ggtggttat cgccagcag cgtgatggc cgatgatgg gacgagggt taagtcggg ggtggggca catgggggc cacccaggg ggtgctcct ggcagtgat gctggagtt tgctgttac gtaaaagtg gcggttttt cagtgaact tgtgtacat attttcgag tggatggtg acgatgtgt agggagtga gctgagtca ggacaaagt taatgttca tctcaaatt gtatatttg cattcaaag taatattaa attaatata cgttaaagt tgcat
