Status
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Gene information
General information
TC032020
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC004922,TC004923
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 4660690..4685834
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032020
    iBeetle screen iB_07827

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032020 RA
      MLAKFWIQC LCSLTVFVQ CSWTAATVS QSTIHDQEY 
      WYEQARIAL RKRLQYATD RRPHAKNVV LFVGDGMGV 
      ATATAARIL RGQRLGKRG EDHELAWDT FPAVAFAKT 
      YNMDAQIGE SSACATALM CGVKTNFET VGLDARGRF 
      ENCFSSFSS RVSSLIDWA QESGKSTGI VTNTRITHA 
      TPAALYGHS PSRYWEDDS KVPPASRKS CKDLARQLI 
      ENDPGRNIN VLLGGGRRH WLPKVAHDP EQTKEEGRR 
      LDGRNLIDD WVRDKKKRG LKAEYVWNK GQLDKINPN 
      QVDYLLGLF AYSHMDFEA DRDAGPSGD PSLADMTRS 
      ALSILLKNP KGFFLFVEG GRIDHAHHY NNAYRALDE 
      TLAMETALL AALALINPT ETLIVVTSD HSHVLTMGG 
      QATPRGHPI LGPDSKVSD IDGQPYTTI LYGNGPGYA 
      TPRIVPMNT TTASEDRNQ VHGSAVPRQ WATHGGEDV 
      PVYALGPLA TTLFTGTFD QSYIPHAIA YSACLAEHR 
      LRCQGIDNY TAPQVTTCV APEVSSVSA AGAPVVIAS 
      SVMADDGTR VKSGVGHMG ATQGVLLAV MLEFAVT
    CDS sequence
    • >TC032020 RA
      atgttggcc aagttttgg atacagtgt ttgtgcagt 
      ttgacagta tttgtgcag tgttcttgg acggctgcc 
      acagttagt caaagtacc attcatgac caggaatat 
      tggtacgaa caagcacga atcgctttg cggaagaga 
      ctgcaatat gctaccgac cgccggcca catgccaag 
      aatgtggtg ttgtttgtg ggtgatggg atgggcgta 
      gccacggcc actgccgcc aggatcctg agaggacaa 
      cggctggga aaaagggga gaggaccac gagctcgcc 
      tgggacacc ttcccggcc gtggccttc gctaagact 
      tacaacatg gacgcccag ataggagag tcatcagca 
      tgcgctacg gcgttgatg tgcggcgtt aaaacaaac 
      tttgaaacg gtcggtttg gatgctcga ggtcgtttc 
      gaaaactgt ttttctagc ttctcttca cgagtctca 
      tcgctgata gattgggca caagagtca gggaaatcg 
      acaggaatt gtgacaaac acccgaata actcacgcc 
      acacccgca gctctgtat ggtcattct ccgtcacgc 
      tattgggag gacgattcg aaagttccg ccagcgtct 
      cgaaaatcc tgcaaggat ttagctcgg caactcatc 
      gaaaatgat cctggacgg aacatcaac gttttgttg 
      ggcggcgga aggcgacac tggttgcca aaagttgcc 
      cacgatcca gaacaaact aaagaggaa ggtcggcgc 
      ctcgatgga cgtaatctc atcgatgac tgggttcga 
      gataagaaa aagcgtgga ctcaaagct gaatacgtc 
      tggaataaa ggacagctc gataaaatt aaccccaat 
      caagtcgac tacttgctc ggtttattc gcttattcg 
      cacatggat tttgaagcc gatcgtgac gctggccct 
      tctggggat ccctccctg gctgatatg acgcgttca 
      gccctttcc atcctgttg aaaaatccg aaaggcttc 
      ttcttgttc gtcgaagga ggacggatc gatcacgcc 
      caccattac aacaacgct tacagggct ttagacgaa 
      acgctggcg atggagacg gcccttctg gcggctctg 
      gccctcata aacccaaca gagacgtta attgttgtt 
      acttcggat cattcgcac gtcctcacg atggggggc 
      caagccacg ccgagagga cacccgatc ctaggtcct 
      gacagcaaa gtatccgac atcgacggc caaccctac 
      accaccatc ctctacggc aacggcccc ggctacgcc 
      acccctcgc atcgtgccc atgaacacc accaccgct 
      tccgaagac cgcaaccag gtgcacggc tcggctgtg 
      ccccggcaa tgggccacc cacggcggc gaggacgtg 
      cccgtctat gcactaggt cccctggcc accaccctc 
      ttcaccggg accttcgac cagagctac atcccgcac 
      gccatcgct tacagcgcc tgtttggcc gaacatcgc 
      ctgcgctgc cagggaatc gacaattac accgccccc 
      caggtgacc acgtgcgtc gctccggaa gtgtcgagc 
      gtttccgct gccggagct ccggtggtt atcgccagc 
      agcgtgatg gccgatgat gggacgagg gttaagtcg 
      ggggtgggg cacatgggg gccacccag ggggtgctc 
      ctggcagtg atgctggag tttgctgtt acgtaa
    mRNA sequence
    • >TC032020 RA
      tcgaaagtg gaattttgt acaaaacac gcacgtagg 
      aaggattta gtgaactaa tcccgcgac atcgtccca 
      ctgcaccga atttggata gacaatgaa tggttgtca 
      ttagttatc cccagaaga taacactaa tcaaatttt 
      gggaggacc tgtttgcaa ccactagtg ttagatcag 
      tgatgatca atttagtgt tctagtcgg agagttttt 
      tgggacaat gttggccaa gttttggat acagtgttt 
      gtgcagttt gacagtatt tgtgcagtg ttcttggac 
      ggctgccac agttagtca aagtaccat tcatgacca 
      ggaatattg gtacgaaca agcacgaat cgctttgcg 
      gaagagact gcaatatgc taccgaccg ccggccaca 
      tgccaagaa tgtggtgtt gtttgtggg tgatgggat 
      gggcgtagc cacggccac tgccgccag gatcctgag 
      aggacaacg gctgggaaa aaggggaga ggaccacga 
      gctcgcctg ggacacctt cccggccgt ggccttcgc 
      taagactta caacatgga cgcccagat aggagagtc 
      atcagcatg cgctacggc gttgatgtg cggcgttaa 
      aacaaactt tgaaacggt cggtttgga tgctcgagg 
      tcgtttcga aaactgttt ttctagctt ctcttcacg 
      agtctcatc gctgataga ttgggcaca agagtcagg 
      gaaatcgac aggaattgt gacaaacac ccgaataac 
      tcacgccac acccgcagc tctgtatgg tcattctcc 
      gtcacgcta ttgggagga cgattcgaa agttccgcc 
      agcgtctcg aaaatcctg caaggattt agctcggca 
      actcatcga aaatgatcc tggacggaa catcaacgt 
      tttgttggg cggcggaag gcgacactg gttgccaaa 
      agttgccca cgatccaga acaaactaa agaggaagg 
      tcggcgcct cgatggacg taatctcat cgatgactg 
      ggttcgaga taagaaaaa gcgtggact caaagctga 
      atacgtctg gaataaagg acagctcga taaaattaa 
      ccccaatca agtcgacta cttgctcgg tttattcgc 
      ttattcgca catggattt tgaagccga tcgtgacgc 
      tggcccttc tggggatcc ctccctggc tgatatgac 
      gcgttcagc cctttccat cctgttgaa aaatccgaa 
      aggcttctt cttgttcgt cgaaggagg acggatcga 
      tcacgccca ccattacaa caacgctta cagggcttt 
      agacgaaac gctggcgat ggagacggc ccttctggc 
      ggctctggc cctcataaa cccaacaga gacgttaat 
      tgttgttac ttcggatca ttcgcacgt cctcacgat 
      ggggggcca agccacgcc gagaggaca cccgatcct 
      aggtcctga cagcaaagt atccgacat cgacggcca 
      accctacac caccatcct ctacggcaa cggccccgg 
      ctacgccac ccctcgcat cgtgcccat gaacaccac 
      caccgcttc cgaagaccg caaccaggt gcacggctc 
      ggctgtgcc ccggcaatg ggccaccca cggcggcga 
      ggacgtgcc cgtctatgc actaggtcc cctggccac 
      caccctctt caccgggac cttcgacca gagctacat 
      cccgcacgc catcgctta cagcgcctg tttggccga 
      acatcgcct gcgctgcca gggaatcga caattacac 
      cgcccccca ggtgaccac gtgcgtcgc tccggaagt 
      gtcgagcgt ttccgctgc cggagctcc ggtggttat 
      cgccagcag cgtgatggc cgatgatgg gacgagggt 
      taagtcggg ggtggggca catgggggc cacccaggg 
      ggtgctcct ggcagtgat gctggagtt tgctgttac 
      gtaaaagtg gcggttttt cagtgaact tgtgtacat 
      attttcgag tggatggtg acgatgtgt agggagtga 
      gctgagtca ggacaaagt taatgttca tctcaaatt 
      gtatatttg cattcaaag taatattaa attaatata 
      cgttaaagt tgcat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY