Status
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Gene information
General information
TC032020
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC004922,TC004923 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 4660690..4685834
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC032020
    iBeetle screen iB_07827

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032020 RA
      MLAKFWIQC LCSLTVFVQ CSWTAATVS QSTIHDQEY 
WYEQARIAL RKRLQYATD RRPHAKNVV LFVGDGMGV 
ATATAARIL RGQRLGKRG EDHELAWDT FPAVAFAKT 
YNMDAQIGE SSACATALM CGVKTNFET VGLDARGRF 
ENCFSSFSS RVSSLIDWA QESGKSTGI VTNTRITHA 
TPAALYGHS PSRYWEDDS KVPPASRKS CKDLARQLI 
ENDPGRNIN VLLGGGRRH WLPKVAHDP EQTKEEGRR 
LDGRNLIDD WVRDKKKRG LKAEYVWNK GQLDKINPN 
QVDYLLGLF AYSHMDFEA DRDAGPSGD PSLADMTRS 
ALSILLKNP KGFFLFVEG GRIDHAHHY NNAYRALDE 
TLAMETALL AALALINPT ETLIVVTSD HSHVLTMGG 
QATPRGHPI LGPDSKVSD IDGQPYTTI LYGNGPGYA 
TPRIVPMNT TTASEDRNQ VHGSAVPRQ WATHGGEDV 
PVYALGPLA TTLFTGTFD QSYIPHAIA YSACLAEHR 
LRCQGIDNY TAPQVTTCV APEVSSVSA AGAPVVIAS 
SVMADDGTR VKSGVGHMG ATQGVLLAV MLEFAVT
    CDS sequence
    • >TC032020 RA
      atgttggcc aagttttgg atacagtgt ttgtgcagt 
ttgacagta tttgtgcag tgttcttgg acggctgcc 
acagttagt caaagtacc attcatgac caggaatat 
tggtacgaa caagcacga atcgctttg cggaagaga 
ctgcaatat gctaccgac cgccggcca catgccaag 
aatgtggtg ttgtttgtg ggtgatggg atgggcgta 
gccacggcc actgccgcc aggatcctg agaggacaa 
cggctggga aaaagggga gaggaccac gagctcgcc 
tgggacacc ttcccggcc gtggccttc gctaagact 
tacaacatg gacgcccag ataggagag tcatcagca 
tgcgctacg gcgttgatg tgcggcgtt aaaacaaac 
tttgaaacg gtcggtttg gatgctcga ggtcgtttc 
gaaaactgt ttttctagc ttctcttca cgagtctca 
tcgctgata gattgggca caagagtca gggaaatcg 
acaggaatt gtgacaaac acccgaata actcacgcc 
acacccgca gctctgtat ggtcattct ccgtcacgc 
tattgggag gacgattcg aaagttccg ccagcgtct 
cgaaaatcc tgcaaggat ttagctcgg caactcatc 
gaaaatgat cctggacgg aacatcaac gttttgttg 
ggcggcgga aggcgacac tggttgcca aaagttgcc 
cacgatcca gaacaaact aaagaggaa ggtcggcgc 
ctcgatgga cgtaatctc atcgatgac tgggttcga 
gataagaaa aagcgtgga ctcaaagct gaatacgtc 
tggaataaa ggacagctc gataaaatt aaccccaat 
caagtcgac tacttgctc ggtttattc gcttattcg 
cacatggat tttgaagcc gatcgtgac gctggccct 
tctggggat ccctccctg gctgatatg acgcgttca 
gccctttcc atcctgttg aaaaatccg aaaggcttc 
ttcttgttc gtcgaagga ggacggatc gatcacgcc 
caccattac aacaacgct tacagggct ttagacgaa 
acgctggcg atggagacg gcccttctg gcggctctg 
gccctcata aacccaaca gagacgtta attgttgtt 
acttcggat cattcgcac gtcctcacg atggggggc 
caagccacg ccgagagga cacccgatc ctaggtcct 
gacagcaaa gtatccgac atcgacggc caaccctac 
accaccatc ctctacggc aacggcccc ggctacgcc 
acccctcgc atcgtgccc atgaacacc accaccgct 
tccgaagac cgcaaccag gtgcacggc tcggctgtg 
ccccggcaa tgggccacc cacggcggc gaggacgtg 
cccgtctat gcactaggt cccctggcc accaccctc 
ttcaccggg accttcgac cagagctac atcccgcac 
gccatcgct tacagcgcc tgtttggcc gaacatcgc 
ctgcgctgc cagggaatc gacaattac accgccccc 
caggtgacc acgtgcgtc gctccggaa gtgtcgagc 
gtttccgct gccggagct ccggtggtt atcgccagc 
agcgtgatg gccgatgat gggacgagg gttaagtcg 
ggggtgggg cacatgggg gccacccag ggggtgctc 
ctggcagtg atgctggag tttgctgtt acgtaa
    mRNA sequence
    • >TC032020 RA
      tcgaaagtg gaattttgt acaaaacac gcacgtagg 
aaggattta gtgaactaa tcccgcgac atcgtccca 
ctgcaccga atttggata gacaatgaa tggttgtca 
ttagttatc cccagaaga taacactaa tcaaatttt 
gggaggacc tgtttgcaa ccactagtg ttagatcag 
tgatgatca atttagtgt tctagtcgg agagttttt 
tgggacaat gttggccaa gttttggat acagtgttt 
gtgcagttt gacagtatt tgtgcagtg ttcttggac 
ggctgccac agttagtca aagtaccat tcatgacca 
ggaatattg gtacgaaca agcacgaat cgctttgcg 
gaagagact gcaatatgc taccgaccg ccggccaca 
tgccaagaa tgtggtgtt gtttgtggg tgatgggat 
gggcgtagc cacggccac tgccgccag gatcctgag 
aggacaacg gctgggaaa aaggggaga ggaccacga 
gctcgcctg ggacacctt cccggccgt ggccttcgc 
taagactta caacatgga cgcccagat aggagagtc 
atcagcatg cgctacggc gttgatgtg cggcgttaa 
aacaaactt tgaaacggt cggtttgga tgctcgagg 
tcgtttcga aaactgttt ttctagctt ctcttcacg 
agtctcatc gctgataga ttgggcaca agagtcagg 
gaaatcgac aggaattgt gacaaacac ccgaataac 
tcacgccac acccgcagc tctgtatgg tcattctcc 
gtcacgcta ttgggagga cgattcgaa agttccgcc 
agcgtctcg aaaatcctg caaggattt agctcggca 
actcatcga aaatgatcc tggacggaa catcaacgt 
tttgttggg cggcggaag gcgacactg gttgccaaa 
agttgccca cgatccaga acaaactaa agaggaagg 
tcggcgcct cgatggacg taatctcat cgatgactg 
ggttcgaga taagaaaaa gcgtggact caaagctga 
atacgtctg gaataaagg acagctcga taaaattaa 
ccccaatca agtcgacta cttgctcgg tttattcgc 
ttattcgca catggattt tgaagccga tcgtgacgc 
tggcccttc tggggatcc ctccctggc tgatatgac 
gcgttcagc cctttccat cctgttgaa aaatccgaa 
aggcttctt cttgttcgt cgaaggagg acggatcga 
tcacgccca ccattacaa caacgctta cagggcttt 
agacgaaac gctggcgat ggagacggc ccttctggc 
ggctctggc cctcataaa cccaacaga gacgttaat 
tgttgttac ttcggatca ttcgcacgt cctcacgat 
ggggggcca agccacgcc gagaggaca cccgatcct 
aggtcctga cagcaaagt atccgacat cgacggcca 
accctacac caccatcct ctacggcaa cggccccgg 
ctacgccac ccctcgcat cgtgcccat gaacaccac 
caccgcttc cgaagaccg caaccaggt gcacggctc 
ggctgtgcc ccggcaatg ggccaccca cggcggcga 
ggacgtgcc cgtctatgc actaggtcc cctggccac 
caccctctt caccgggac cttcgacca gagctacat 
cccgcacgc catcgctta cagcgcctg tttggccga 
acatcgcct gcgctgcca gggaatcga caattacac 
cgcccccca ggtgaccac gtgcgtcgc tccggaagt 
gtcgagcgt ttccgctgc cggagctcc ggtggttat 
cgccagcag cgtgatggc cgatgatgg gacgagggt 
taagtcggg ggtggggca catgggggc cacccaggg 
ggtgctcct ggcagtgat gctggagtt tgctgttac 
gtaaaagtg gcggttttt cagtgaact tgtgtacat 
attttcgag tggatggtg acgatgtgt agggagtga 
gctgagtca ggacaaagt taatgttca tctcaaatt 
gtatatttg cattcaaag taatattaa attaatata 
cgttaaagt tgcat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY