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Gene information
General information
TC004470 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 4580551..4598738
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004470
    iBeetle screen iB_07824

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004470 RA
      MNTSSTENT SVTYAPHEA TYSITQVVF IGTIAGILS 
      VVTVVGNIM VMISFKIDK QLQTISNYF LFSLAVADF 
      AIGLISMPL FTVSTLLGY WPLGPLVCD TWLALDYLA 
      SNASVLNLL IISFDRYFS VTRPLTYRA KRTTNRAAI 
      MIACAWGIS LLLWPPWIY SWPYIEGER TVPENECYI 
      QFIETNHYI TFGTAIAAF YVPVTVMCF LYWRIWRET 
      EKRQKDLPN LQAGKKDSS KRSNSSDEA NNACSTVDL 
      EDWRRPRSE SSTGADTDS VYMHSTVCV DHHRRKRPR 
      KGGWLAGVW RIRAWCVAW WHSGREDSD TEEESPSEQ 
      GQGCITPVS LDTPLQSSV SRCTSLNVI RDPYVSTLS 
      TQRELPHAQ ANMTPTRLA PRDSRSLPT GNRLAGRSV 
      SSDSVYTIL IRLPGDPGE GTGCSEAAS IKMIPEDVR 
      TRSHPDVDY SMNTLHANH LNRRPSAMP DIRIPLNAK 
      IIPKQLAGK TLLANATKN QGKKKKKTQ EKKADKKAA 
      KTLSAILLT FIITWTPYN VLVLLKPVT ASSARIPPQ 
      LWDFFYYLC YINSTINPV CYALCNASF RRTYLRILQ 
      CKWHNRNRA AMNRGFYN
    CDS sequence
    • >TC004470 RA
      atgaacacg tcgtcgacg gagaacaca tctgtcacc 
      tacgctccg cacgaggcc acctattcc attacccaa 
      gtcgtcttt attggtacc atagccgga atcctgtct 
      gtggtaaca gtggtaggc aacataatg gtgatgatt 
      tccttcaag atcgacaag caactgcag acgataagc 
      aactacttc ctcttctcc ttggctgtg gccgatttt 
      gcgattggt ctaatttcc atgcctctg tttaccgtg 
      tccacactt ctgggctac tggcctctg ggacccctc 
      gtctgcgac acttggctg gctctcgat tacctcgct 
      agtaatgcc tcggtgctc aacttactc atcatcagc 
      tttgatcgc tatttttcg gtgaccaga cctctcact 
      taccgtgcc aaaaggacc accaacaga gccgccatc 
      atgatagca tgcgcttgg ggtattagt ttgcttttg 
      tggccaccc tggatctac agctggcca tatatcgaa 
      ggcgagcgg accgtacct gagaacgag tgctacatt 
      caattcatc gaaaccaac cactacatc actttcgga 
      acagcaatt gccgctttc tatgttccc gtcaccgtt 
      atgtgcttt ctatactgg cgtatatgg cgggaaacg 
      gaaaagagg caaaaggac ttacccaac ctgcaagcg 
      ggcaaaaaa gacagcagc aaacggagc aattccagt 
      gatgaagca aataatgcc tgttcgacg gtggattta 
      gaagactgg agaaggcct cgatccgag tcttcaacg 
      ggcgccgac acagattct gtttatatg cacagtaca 
      gtctgcgtc gatcatcat cgacggaaa agaccgaga 
      aaaggcggt tggttggcg ggagtgtgg aggataaga 
      gcgtggtgt gtggcgtgg tggcactcc ggacgggaa 
      gacagcgac acagaagaa gaaagtcca agtgaacag 
      ggccaaggc tgcatcact ccggtgtct ttggatacg 
      cctttgcaa agctcggtg tcgagatgc acttcgctg 
      aacgtcatc agggacccg tacgtcagc acgttgtcg 
      acccagcgc gaattaccg cacgctcag gccaatatg 
      accccaact cgtctcgcg ccgagggac tccagaagc 
      ttgccgacg ggcaaccgt ttggcaggt cgttcagtt 
      tcaagcgat agcgtttac acgatcctg atccgcttg 
      cccggcgat cccggcgaa ggcaccggc tgctcggaa 
      gcggcctcg atcaagatg atcccggag gacgtgcgc 
      acgcgatcc caccccgac gtggactac tcgatgaac 
      acgctgcac gccaaccac ctcaatcgg cgtccgtcg 
      gccatgccc gacatccgg ataccgctc aacgccaag 
      atcatcccc aagcagctg gccggcaag acgctgctg 
      gccaacgcc acgaaaaac caaggcaag aagaagaag 
      aagacgcag gagaagaag gccgacaag aaggcggcc 
      aagaccctg tctgcgatt ctgctcact tttatcatc 
      acctggacg ccgtacaac gtcttggtg ctgctgaag 
      ccggtgacg gcgtcgagt gcccgcatc ccgccccag 
      ctgtgggat ttcttttac tacctgtgc tacattaac 
      tctaccatc aacccggtg tgctacgct ctgtgcaac 
      gccagtttt cggcgaacg tacctcaga atcctccag 
      tgtaaatgg cacaatagg aaccgcgcc gccatgaat 
      cgaggcttt tacaattaa
    mRNA sequence
    • >TC004470 RA
      cagcttgca ggagcccca ggagtctgt gtgtgatgt 
      gcccgctga cgatgagac atggccaag cgagatgta 
      accgctgag aaagggcgg agcaaaatg cggcggcta 
      gcctgcagg tcctcgtga caacatgaa cacgtcgtc 
      gacggagaa cacatctgt cacctacgc tccgcacga 
      ggccaccta ttccattac ccaagtcgt ctttattgg 
      taccatagc cggaatcct gtctgtggt aacagtggt 
      aggcaacat aatggtgat gatttcctt caagatcga 
      caagcaact gcagacgat aagcaacta cttcctctt 
      ctccttggc tgtggccga ttttgcgat tggtctaat 
      ttccatgcc tctgtttac cgtgtccac acttctggg 
      ctactggcc tctgggacc cctcgtctg cgacacttg 
      gctggctct cgattacct cgctagtaa tgcctcggt 
      gctcaactt actcatcat cagctttga tcgctattt 
      ttcggtgac cagacctct cacttaccg tgccaaaag 
      gaccaccaa cagagccgc catcatgat agcatgcgc 
      ttggggtat tagtttgct tttgtggcc accctggat 
      ctacagctg gccatatat cgaaggcga gcggaccgt 
      acctgagaa cgagtgcta cattcaatt catcgaaac 
      caaccacta catcacttt cggaacagc aattgccgc 
      tttctatgt tcccgtcac cgttatgtg ctttctata 
      ctggcgtat atggcggga aacggaaaa gaggcaaaa 
      ggacttacc caacctgca agcgggcaa aaaagacag 
      cagcaaacg gagcaattc cagtgatga agcaaataa 
      tgcctgttc gacggtgga tttagaaga ctggagaag 
      gcctcgatc cgagtcttc aacgggcgc cgacacaga 
      ttctgttta tatgcacag tacagtctg cgtcgatca 
      tcatcgacg gaaaagacc gagaaaagg cggttggtt 
      ggcgggagt gtggaggat aagagcgtg gtgtgtggc 
      gtggtggca ctccggacg ggaagacag cgacacaga 
      agaagaaag tccaagtga acagggcca aggctgcat 
      cactccggt gtctttgga tacgccttt gcaaagctc 
      ggtgtcgag atgcacttc gctgaacgt catcaggga 
      cccgtacgt cagcacgtt gtcgaccca gcgcgaatt 
      accgcacgc tcaggccaa tatgacccc aactcgtct 
      cgcgccgag ggactccag aagcttgcc gacgggcaa 
      ccgtttggc aggtcgttc agtttcaag cgatagcgt 
      ttacacgat cctgatccg cttgcccgg cgatcccgg 
      cgaaggcac cggctgctc ggaagcggc ctcgatcaa 
      gatgatccc ggaggacgt gcgcacgcg atcccaccc 
      cgacgtgga ctactcgat gaacacgct gcacgccaa 
      ccacctcaa tcggcgtcc gtcggccat gcccgacat 
      ccggatacc gctcaacgc caagatcat ccccaagca 
      gctggccgg caagacgct gctggccaa cgccacgaa 
      aaaccaagg caagaagaa gaagaagac gcaggagaa 
      gaaggccga caagaaggc ggccaagac cctgtctgc 
      gattctgct cacttttat catcacctg gacgccgta 
      caacgtctt ggtgctgct gaagccggt gacggcgtc 
      gagtgcccg catcccgcc ccagctgtg ggatttctt 
      ttactacct gtgctacat taactctac catcaaccc 
      ggtgtgcta cgctctgtg caacgccag ttttcggcg 
      aacgtacct cagaatcct ccagtgtaa atggcacaa 
      taggaaccg cgccgccat gaatcgagg cttttacaa 
      ttaacgttg aggaactgc ggatgtact ttggccggt 
      aaaaggcca tttccgacg tttgtgcaa agtgggttt 
      tagccggca acaactccc cggactatt tctagatgt 
      ttttatatt tgtaattgt ataccgctc aaaccgtgt 
      tctaaaatc gtgcgtgag gactgaaat tgtagcttt 
      aagcttgga ccaaagtgg ggacgatcg cctacactc 
      aaacgagtg ttcacattt ggacaactt agcttcagg 
      cgaccgctc ccaccgtgg tgcaacctt aacaacaac 
      aaaaagata tttatacat aatatagag gctgtgtgt 
      tagatttag tcttatttg ttcgtggca agaccaatc 
      tttggacat ccatagcat taatgacgg gctgatgac 
      ggctgtctt ccaccggag aactggcag gaccaggaa 
      tcgtcgtca caacttcaa aacttagca agactttgt 
      cttttaccc aacgacggt ccagtaaaa ctgaaacga 
      agaaataat acacatcat tcccctgtc gaaaatcct 
      attttctgt gcctgctca ctctgtcaa ctgtattat 
      ttattcagt tcaggaaac agcaccagg acctgtctg 
      gagcttcgt agcgcctca atgtatagc gattatgtt 
      tagaagcaa taatcagcg aatatacaa tacaataat 
      tttcttccc atcccgatg atgaacaat gtgccaaat 
      tatcgcaac gatcataca taggtgtca actgtgaat 
      aaattccaa tttccattg caatattaa ttcgtgtta 
      tttattgga agaaacata tgtactttg acaatctct 
      atcatctcg atgttacgc tcccataca cctggatgt 
      tttcgcctg gcccttgtt tgtactacg acggaatta 
      tattaattc gtttgttat ttcgttcaa gaaatttag 
      tttaatcgt gtgatcatt tcatcaaat gactcctca 
      cgaagattt attaaatac aaacgccca gtcttgttt 
      tcatcgatc tttgctcca gtgccaacc tagaagtga 
      tatttgata caggtatta agtaagacg caaatttgc 
      tttgatccc attctctcg tatttaata aatcatcgt 
      gtagtaaga cacatgggg ttcccaaag tggcattgt 
      aaaggaggc aagggtcac cttggttcc ttgtctggg 
      acgatttta ataccgacc caatgggac tttcaatgt 
      aactatcga cgcacatta ggtacaatt tattataaa 
      ataaaacca caaggaacg agacttttg taatattta 
      ccattcccg atataaata gtggtttgt aaatgatgt 
      taataaata aaatgttgt tttatat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY