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Gene information
General information
TC005009 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 662188..685552
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005009
    iBeetle screen iB_07815

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    previtellogenic egg chamber number increased - Number of animals showing the phenotype: 2

    previtellogenic egg chamber stage sequence irregular - Number of animals showing the phenotype: 2
    mature egg not present - Number of animals showing the phenotype: 2
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 prothorax musculature specific subset oblique posterior number decreased - Penetrance (percentage of animals/eggs on slide): more than 80%

    abdomen musculature random rounded - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005009 RA
      MSDDSDFEP RNKRRRNIR CLNDSGSSS ASSSVVTTT 
      RKGIRRRFV EDSSDSDSE SSIEARPKR RVIPRVTSD 
      PESDSSGSV IHRPKRTLR VVSDSSDDS DHGFEGSSS 
      SQWETDVSD VETPAKIQD TGVDSDSSD GQSEKCPIC 
      LISFTNQEI GTPESCDHL FCVDCIQEW SKNVNTCPV 
      DRQEFRLIL VRKNLNGSI YREIQVQPP APQNEVDIL 
      EDPTFCEIC GSSDREERM LLCDGCDLG FHMECLTPP 
      LADIPPGAW FCNDCTPED LVDAEIELY ELQLLLDDA 
      NEVGRPRSS RRTTTTSRL VPRTRQSER VRQRIANNR 
      NNNEASDER PQPVSSTSR PVRRVKKKK RRKKKPRYR 
      VVYQIDSTT GETIAVKKR RKKRRTKPV PKKTTVKKR 
      LAQQLGICA PRNVPQNLP DVRTQPQTN SLQTQRYHA 
      GIPTLNIFG NRDELDFVV SEEDDDFGD GGVVLRRGI 
      TRRRTRIKS ITVPNSVSS STDVLGSIL DVQSRFHSK 
      HTKFSLGQD GKFTMENTK DRLKDLNNW NQVVRQRPI 
      SQETSDQPT SSGHRSSSP EHNNTAPTS TVENSNVSA 
      ISSGNIQEQ YNNPQDYTQ SNLNTIYDV TMEEKRVDK 
      KKKEDASED NTPRSGTPA SESEIDIYS DIETVTTSK 
      VDEADFKAP SPLPATPNT GTDDDNNDS EPDMVIDTD 
      KPEETTVTS TVDSNSEIT PTAQDVSSS SEIQSQAIQ 
      SSNLLFDDK KYEEEKCVD DDDSEDGCP NFSIYSKET 
      ITMAKTITE DAKPEENLE NDQVGEVSS SPATKTKEP 
      VKKSTGLYS DSEDESIVK KENSFGITD LRNMTEDIS 
      EEERSYTPC LDERPHHCI EGLDTEMIS DEDRNDFDE 
      SHDLKTASD GDALEINAK ESELDFTRP EDYEEGEIV 
      DKSKVKKET KEPVKKKDE TDKSGNKEN ENQNVKQPE 
      FKKLSKSNK ERNYRDKDR ERSKDKDSK KDKKKEKRK 
      EIERYDVRA LIAEKPRRL ITKDKFGRD VRRSPSGSV 
      TKTPPRRTK SPSPKVRRS VSKARSTSR ERSRKKARK 
      RRSVSRSKS RSRSKTKKR RRSASRERR RKSRSKGKS 
      KKHRSRSRH RTRSPTPKR TKEWSRRHS RDWTPSLSR 
      SRTPEPRHL TPSWTPPRI MDNTVKPHN LTVILTNDA 
      NKKKKEKKR KSEKKTKEN ERAKKRKRN ERTPPPSKE 
      VFASGDNIL VSVSFNKDN ETRDVSTKK KREVVEEVK 
      KVKKDKTKK KKKNNRDLA GVKPVAIID LERSPFKEL 
      TPSPKDIIV LSDSDNEEA RNKLQKTIC DSSQQVTSP 
      VNNYTTGPK TPPEPQVKF TLTSKQTQM RAISNPLHD 
      PEDIEPTGD ETQENVEHK GPNTPPEPP NSPPSSPDA 
      YDPFEPTKS RSPTPEVAP SSNALNIDN NRDESITII 
      STEVEKTDQ TPDQKSHTP PLADVQPAD SQSSIQVTP 
      ESSLSKSPE RPVTNATQP VAQTIPFSS SVPTSLMTS 
      TPVNSLAPP RINILSTTL LAPVSIPQR NVLPNANAT 
      SKPARKSSN RNGSDVADT NLGFDSPYS PGSSDYEDL 
      FEPPVETAP KTVTKTTKN VKSPSKVQN AFDALFGTS 
      PFNKVKKQD SKKFSKKAP PAAVPSGGK GTKQVGVKI 
      DEDNLKILD DLPNSAVEM QVKDKFLKK LNRQERVVE 
      EVKLVLKPH YNKKHINKE EYKDILRRA VPKICHNKT 
      GEINPTKIK NLIEAYVKK VRHSKKVTS SSSSVNPQK 
      A
    CDS sequence
    • >TC005009 RA
      atgagtgat gatagtgat ttcgaacct cgaaataag 
      cgcagacga aatattcgt tgtttaaac gacagtgga 
      agcagttct gcaagtagt tcagtagtt acaacaaca 
      cgaaaaggc atcagacga cgatttgtt gaagacagc 
      tcggactca gacagtgag agttcgatt gaagcccgc 
      cctaaacga agagttatt cctagagta acaagtgac 
      ccagaatcg gacagttca ggctctgtt atccatcgt 
      cctaaacgg acgcttcga gtggtcagc gattcgagt 
      gacgactcg gaccatggc tttgaaggc agttcgagc 
      agccaatgg gaaacagac gtttcagac gtcgaaact 
      ccagctaaa attcaagac actggagtc gattccgac 
      tcatccgat ggccaatca gaaaaatgc ccaatttgt 
      ctcatctcg ttcaccaac caagaaatc ggaacgcct 
      gaatcatgc gaccacttg ttctgcgtt gattgcatc 
      caagagtgg tccaaaaac gtcaacact tgcccagtg 
      gatagacaa gagtttcgc ctcatttta gtgcgaaaa 
      aatttaaac gggagcatt tatcgcgaa atacaagtg 
      caaccaccg gctccacag aatgaggtt gacattctt 
      gaagatccg actttttgc gagatctgt ggttcgagt 
      gatagggaa gagaggatg cttctgtgt gatggatgc 
      gacttgggg tttcatatg gagtgtttg acgccacct 
      cttgcggat attccacca ggtgcatgg ttttgtaat 
      gattgtaca ccggaggat ttggttgat gctgaaatc 
      gagttgtat gagcttcag ttgcttcta gatgatgct 
      aatgaagtt gggaggccg agaagtagc cgcaggacg 
      acaacaaca tcgagatta gtacctcgg actcgccaa 
      agtgaaagg gttcgccaa cgaatagca aataaccga 
      aacaacaat gaagcatct gatgaacgc cctcaacct 
      gtttcaagt acatcacgt cctgtccga agagtaaag 
      aagaagaaa cggaggaag aagaagcca cgttatcgt 
      gtcgtttat cagatagat agtaccact ggtgaaaca 
      atcgctgtg aaaaaacgc cgaaagaaa cgacgaaca 
      aaaccagtc ccaaaaaaa accactgta aaaaaacga 
      ttagcccaa caattgggt atttgtgca ccgagaaac 
      gtcccacag aacttaccc gacgtgaga acacaacca 
      caaacaaac agcttacaa acacaacgc taccatgca 
      ggaatacca acgttaaat atttttgga aatcgggac 
      gaattggat tttgtggta tctgaagaa gatgatgat 
      tttggtgat ggtggtgtt gtgcttaga cgtggaata 
      acacgtcga agaacacgt attaaaagt ataactgtt 
      ccgaattcg gtttcaagc agtaccgat gttttgggg 
      agcatttta gacgtccaa tcacgattc cattcgaaa 
      catacgaag ttttccctt ggtcaggat ggtaaattt 
      acgatggaa aacacaaaa gatagattg aaggattta 
      aataattgg aatcaagtc gtaaggcaa cgaccgatt 
      tcgcaggaa actagcgac caacctaca tcaagtggg 
      cacaggagt tccagtcct gagcataac aacacagct 
      ccgacatct accgttgaa aatagcaac gtttccgcc 
      atttcgtct ggaaacatc caggaacag tacaataac 
      ccacaggac tacacacag agcaatctc aatactatt 
      tatgatgtg acaatggag gagaaaaga gtagataag 
      aaaaaaaag gaagatgca tctgaagac aatacacca 
      aggagtggg acaccggca agtgaatcg gaaattgac 
      atttacagt gatattgaa actgtgact actagtaaa 
      gttgacgaa gctgatttc aaagcacca tcacctcta 
      cctgcaaca cctaacacc ggaaccgat gatgataat 
      aatgatagt gaaccagac atggttata gatacggat 
      aaacctgaa gaaacaaca gttacctca acggttgac 
      tctaactca gaaataaca ccaacggca caagacgta 
      tcttcatcg tccgaaatc cagtcacaa gcaatccag 
      tcgtcgaac ctacttttc gacgataaa aaatacgaa 
      gaagagaag tgtgttgat gatgatgat tcggaagat 
      ggatgtcct aattttagt atatattcc aaagaaaca 
      ataactatg gctaaaact ataacagag gacgcaaaa 
      ccggaggaa aacctcgag aatgatcaa gtgggtgaa 
      gtgagttct tcaccggcc accaaaaca aaagaacct 
      gtaaaaaaa agtacaggt ctatatagt gattctgag 
      gatgagtca atagttaag aaggaaaat tcgtttgga 
      atcaccgat ttacgcaac atgactgaa gacatcagt 
      gaggaggaa cgcagttat acgccgtgt cttgacgaa 
      agaccgcat cattgtatc gaaggtttg gatacggaa 
      atgatatca gatgaagac cggaacgat tttgatgaa 
      tctcacgat ttaaaaacg gcctctgac ggcgatgcg 
      cttgaaatc aatgcgaaa gaatcggaa ctagatttt 
      acccgacct gaggattac gaagaaggt gaaatcgtc 
      gataaatca aaggttaag aaagaaaca aaagaaccg 
      gtgaaaaag aaggacgaa acggacaaa tccggaaat 
      aaggaaaac gaaaatcag aatgtcaaa caacctgag 
      tttaaaaaa ctgagtaaa agtaacaag gagcgcaac 
      tatcgcgat aaagaccgc gaacggagt aaggacaaa 
      gactcgaaa aaagataaa aagaaggag aaacgtaag 
      gaaatcgag cggtatgat gttagggcg ttaatagcc 
      gagaagccg cgacgtttg attacgaaa gataagttt 
      gggcgtgat gttagaagg agtccgtcg gggtctgtg 
      acgaagacg ccgcctaga cgtacgaag tcgccgtcg 
      cctaaagtt cgtcggtcc gtttctaaa gcgaggtct 
      acgtcgcgt gaaagaagt cgcaagaag gcgaggaaa 
      cggcggtcg gtgtcgagg tccaagtca cggtcgagg 
      tctaaaacg aagaagaga cggcggtcg gcaagcagg 
      gagaggagg aggaaatca cgatcgaaa ggtaaaagc 
      aaaaaacac cgaagtcgc agtagacac cgtaccagg 
      tctcccaca cccaaacga acaaaagaa tggtctcga 
      agacattca agagattgg acaccttca ttatcacga 
      tcgcgaact cctgaaccg agacactta accccatca 
      tggacaccc ccaagaatt atggataat accgtaaaa 
      ccacacaac ttaactgtg attttaacc aacgatgcc 
      aataagaaa aagaaggaa aagaaacgc aaatctgag 
      aaaaagaca aaggaaaat gaacgtgca aaaaaacgg 
      aaacgtaac gaacgtaca ccgccacct tccaaagaa 
      gtgtttgct tcaggcgat aatattcta gttagtgtg 
      agtttcaac aaagataac gaaacacga gacgtttcg 
      acgaagaag aaacgcgaa gttgtcgaa gaagttaag 
      aaggttaaa aaagacaaa acgaagaag aaaaagaag 
      aataatcgt gatttggct ggagttaaa ccggtggcg 
      attattgat ttggaaaga tcaccgttt aaagaattg 
      acaccttca ccgaaagac attatcgtg ttgagcgat 
      agtgataat gaagaagct aggaacaag ttacagaaa 
      acaatttgt gattcgtca caacaagta accagtccg 
      gttaataat tacacaact ggtcccaaa acaccacca 
      gaacctcaa gttaagttc actttaacc tccaaacaa 
      acacagatg cgtgcaatt tcgaatccg ttacatgac 
      cctgaagat atcgaacca acgggtgac gaaacccag 
      gaaaatgtg gaacataaa ggtcccaac acacctcca 
      gaacctcca aattcacct ccaagttct ccagatgcc 
      tacgatccg ttcgaacca acaaaatca cgatcacca 
      acacctgaa gtagctcca agcagcaat gcgttaaat 
      attgacaat aatcgtgac gaaagtatt accattatt 
      agtacagag gtggaaaaa acggatcaa acgcctgat 
      caaaaatca catacgcca cctttggcc gatgtacaa 
      ccagccgac tcgcagagt agcatccaa gtcactccc 
      gaatcaagc ttgagtaaa tcccccgaa cgtcccgta 
      acaaatgcc acacaacca gttgctcaa acaattccg 
      ttttcttcg tcagtaccg acgtcgtta atgacttcg 
      acacctgtt aatagttta gcacctcca aggattaat 
      attttgagt acgaccctt ttggcgccg gtgtccatc 
      ccgcaaagg aatgttttg ccaaatgcc aatgcaaca 
      tcgaaacct gcaaggaag tcgagtaat cggaacgga 
      agtgatgtt gctgatacg aatttaggg tttgattcg 
      ccctattcg ccaggatct agtgattat gaggatttg 
      tttgaaccg ccagttgaa acagcaccg aagactgtg 
      acgaaaacg acgaaaaat gttaaatca ccgtcaaag 
      gtgcagaat gcgtttgat gcacttttt ggaacatcg 
      ccgtttaat aaagttaag aaacaggac agtaagaag 
      tttagcaag aaggcgccg cctgctgct gttccgagt 
      gggggaaaa ggtactaaa caagtcgga gttaaaata 
      gatgaagac aacttgaag attctcgat gatctgccg 
      aattccgcc gtcgaaatg caagtaaag gacaagttt 
      ttgaagaaa ctgaaccga caagagcga gtcgttgaa 
      gaggttaaa ttggtctta aagccgcat tataacaag 
      aaacatata aataaggag gagtacaag gatatctta 
      agacgggct gttccaaag atttgtcac aacaaaacc 
      ggcgaaata aatccgaca aaaatcaag aatctgatt 
      gaagcatat gtgaaaaaa gtgagacat agtaaaaag 
      gtgacctcc agtagtagt tccgtcaat ccccagaaa 
      gcttaa
    mRNA sequence
    • >TC005009 RA
      ctacggtac agtggctac gactttttg ctttttctg 
      tcattggaa atcttgttt gttatgagt agaaacagt 
      ttaaactga aattcgcta aaataaatc gatagataa 
      gttaatctg gaaatctgt aagtaattg aaaccggtc 
      gcttcacct tattatgta ttattgtaa cttggattt 
      gaaaaatga gtgatgata gtgatttcg aacctcgaa 
      ataagcgca gacgaaata ttcgttgtt taaacgaca 
      gtggaagca gttctgcaa gtagttcag tagttacaa 
      caacacgaa aaggcatca gacgacgat ttgttgaag 
      acagctcgg actcagaca gtgagagtt cgattgaag 
      cccgcccta aacgaagag ttattccta gagtaacaa 
      gtgacccag aatcggaca gttcaggct ctgttatcc 
      atcgtccta aacggacgc ttcgagtgg tcagcgatt 
      cgagtgacg actcggacc atggctttg aaggcagtt 
      cgagcagcc aatgggaaa cagacgttt cagacgtcg 
      aaactccag ctaaaattc aagacactg gagtcgatt 
      ccgactcat ccgatggcc aatcagaaa aatgcccaa 
      tttgtctca tctcgttca ccaaccaag aaatcggaa 
      cgcctgaat catgcgacc acttgttct gcgttgatt 
      gcatccaag agtggtcca aaaacgtca acacttgcc 
      cagtggata gacaagagt ttcgcctca ttttagtgc 
      gaaaaaatt taaacggga gcatttatc gcgaaatac 
      aagtgcaac caccggctc cacagaatg aggttgaca 
      ttcttgaag atccgactt tttgcgaga tctgtggtt 
      cgagtgata gggaagaga ggatgcttc tgtgtgatg 
      gatgcgact tggggtttc atatggagt gtttgacgc 
      cacctcttg cggatattc caccaggtg catggtttt 
      gtaatgatt gtacaccgg aggatttgg ttgatgctg 
      aaatcgagt tgtatgagc ttcagttgc ttctagatg 
      atgctaatg aagttggga ggccgagaa gtagccgca 
      ggacgacaa caacatcga gattagtac ctcggactc 
      gccaaagtg aaagggttc gccaacgaa tagcaaata 
      accgaaaca acaatgaag catctgatg aacgccctc 
      aacctgttt caagtacat cacgtcctg tccgaagag 
      taaagaaga agaaacgga ggaagaaga agccacgtt 
      atcgtgtcg tttatcaga tagatagta ccactggtg 
      aaacaatcg ctgtgaaaa aacgccgaa agaaacgac 
      gaacaaaac cagtcccaa aaaaaacca ctgtaaaaa 
      aacgattag cccaacaat tgggtattt gtgcaccga 
      gaaacgtcc cacagaact tacccgacg tgagaacac 
      aaccacaaa caaacagct tacaaacac aacgctacc 
      atgcaggaa taccaacgt taaatattt ttggaaatc 
      gggacgaat tggattttg tggtatctg aagaagatg 
      atgattttg gtgatggtg gtgttgtgc ttagacgtg 
      gaataacac gtcgaagaa cacgtatta aaagtataa 
      ctgttccga attcggttt caagcagta ccgatgttt 
      tggggagca ttttagacg tccaatcac gattccatt 
      cgaaacata cgaagtttt cccttggtc aggatggta 
      aatttacga tggaaaaca caaaagata gattgaagg 
      atttaaata attggaatc aagtcgtaa ggcaacgac 
      cgatttcgc aggaaacta gcgaccaac ctacatcaa 
      gtgggcaca ggagttcca gtcctgagc ataacaaca 
      cagctccga catctaccg ttgaaaata gcaacgttt 
      ccgccattt cgtctggaa acatccagg aacagtaca 
      ataacccac aggactaca cacagagca atctcaata 
      ctatttatg atgtgacaa tggaggaga aaagagtag 
      ataagaaaa aaaaggaag atgcatctg aagacaata 
      caccaagga gtgggacac cggcaagtg aatcggaaa 
      ttgacattt acagtgata ttgaaactg tgactacta 
      gtaaagttg acgaagctg atttcaaag caccatcac 
      ctctacctg caacaccta acaccggaa ccgatgatg 
      ataataatg atagtgaac cagacatgg ttatagata 
      cggataaac ctgaagaaa caacagtta cctcaacgg 
      ttgactcta actcagaaa taacaccaa cggcacaag 
      acgtatctt catcgtccg aaatccagt cacaagcaa 
      tccagtcgt cgaacctac ttttcgacg ataaaaaat 
      acgaagaag agaagtgtg ttgatgatg atgattcgg 
      aagatggat gtcctaatt ttagtatat attccaaag 
      aaacaataa ctatggcta aaactataa cagaggacg 
      caaaaccgg aggaaaacc tcgagaatg atcaagtgg 
      gtgaagtga gttcttcac cggccacca aaacaaaag 
      aacctgtaa aaaaaagta caggtctat atagtgatt 
      ctgaggatg agtcaatag ttaagaagg aaaattcgt 
      ttggaatca ccgatttac gcaacatga ctgaagaca 
      tcagtgagg aggaacgca gttatacgc cgtgtcttg 
      acgaaagac cgcatcatt gtatcgaag gtttggata 
      cggaaatga tatcagatg aagaccgga acgattttg 
      atgaatctc acgatttaa aaacggcct ctgacggcg 
      atgcgcttg aaatcaatg cgaaagaat cggaactag 
      attttaccc gacctgagg attacgaag aaggtgaaa 
      tcgtcgata aatcaaagg ttaagaaag aaacaaaag 
      aaccggtga aaaagaagg acgaaacgg acaaatccg 
      gaaataagg aaaacgaaa atcagaatg tcaaacaac 
      ctgagttta aaaaactga gtaaaagta acaaggagc 
      gcaactatc gcgataaag accgcgaac ggagtaagg 
      acaaagact cgaaaaaag ataaaaaga aggagaaac 
      gtaaggaaa tcgagcggt atgatgtta gggcgttaa 
      tagccgaga agccgcgac gtttgatta cgaaagata 
      agtttgggc gtgatgtta gaaggagtc cgtcggggt 
      ctgtgacga agacgccgc ctagacgta cgaagtcgc 
      cgtcgccta aagttcgtc ggtccgttt ctaaagcga 
      ggtctacgt cgcgtgaaa gaagtcgca agaaggcga 
      ggaaacggc ggtcggtgt cgaggtcca agtcacggt 
      cgaggtcta aaacgaaga agagacggc ggtcggcaa 
      gcagggaga ggaggagga aatcacgat cgaaaggta 
      aaagcaaaa aacaccgaa gtcgcagta gacaccgta 
      ccaggtctc ccacaccca aacgaacaa aagaatggt 
      ctcgaagac attcaagag attggacac cttcattat 
      cacgatcgc gaactcctg aaccgagac acttaaccc 
      catcatgga cacccccaa gaattatgg ataataccg 
      taaaaccac acaacttaa ctgtgattt taaccaacg 
      atgccaata agaaaaaga aggaaaaga aacgcaaat 
      ctgagaaaa agacaaagg aaaatgaac gtgcaaaaa 
      aacggaaac gtaacgaac gtacaccgc caccttcca 
      aagaagtgt ttgcttcag gcgataata ttctagtta 
      gtgtgagtt tcaacaaag ataacgaaa cacgagacg 
      tttcgacga agaagaaac gcgaagttg tcgaagaag 
      ttaagaagg ttaaaaaag acaaaacga agaagaaaa 
      agaagaata atcgtgatt tggctggag ttaaaccgg 
      tggcgatta ttgatttgg aaagatcac cgtttaaag 
      aattgacac cttcaccga aagacatta tcgtgttga 
      gcgatagtg ataatgaag aagctagga acaagttac 
      agaaaacaa tttgtgatt cgtcacaac aagtaacca 
      gtccggtta ataattaca caactggtc ccaaaacac 
      caccagaac ctcaagtta agttcactt taacctcca 
      aacaaacac agatgcgtg caatttcga atccgttac 
      atgaccctg aagatatcg aaccaacgg gtgacgaaa 
      cccaggaaa atgtggaac ataaaggtc ccaacacac 
      ctccagaac ctccaaatt cacctccaa gttctccag 
      atgcctacg atccgttcg aaccaacaa aatcacgat 
      caccaacac ctgaagtag ctccaagca gcaatgcgt 
      taaatattg acaataatc gtgacgaaa gtattacca 
      ttattagta cagaggtgg aaaaaacgg atcaaacgc 
      ctgatcaaa aatcacata cgccacctt tggccgatg 
      tacaaccag ccgactcgc agagtagca tccaagtca 
      ctcccgaat caagcttga gtaaatccc ccgaacgtc 
      ccgtaacaa atgccacac aaccagttg ctcaaacaa 
      ttccgtttt cttcgtcag taccgacgt cgttaatga 
      cttcgacac ctgttaata gtttagcac ctccaagga 
      ttaatattt tgagtacga cccttttgg cgccggtgt 
      ccatcccgc aaaggaatg ttttgccaa atgccaatg 
      caacatcga aacctgcaa ggaagtcga gtaatcgga 
      acggaagtg atgttgctg atacgaatt tagggtttg 
      attcgccct attcgccag gatctagtg attatgagg 
      atttgtttg aaccgccag ttgaaacag caccgaaga 
      ctgtgacga aaacgacga aaaatgtta aatcaccgt 
      caaaggtgc agaatgcgt ttgatgcac tttttggaa 
      catcgccgt ttaataaag ttaagaaac aggacagta 
      agaagttta gcaagaagg cgccgcctg ctgctgttc 
      cgagtgggg gaaaaggta ctaaacaag tcggagtta 
      aaatagatg aagacaact tgaagattc tcgatgatc 
      tgccgaatt ccgccgtcg aaatgcaag taaaggaca 
      agtttttga agaaactga accgacaag agcgagtcg 
      ttgaagagg ttaaattgg tcttaaagc cgcattata 
      acaagaaac atataaata aggaggagt acaaggata 
      tcttaagac gggctgttc caaagattt gtcacaaca 
      aaaccggcg aaataaatc cgacaaaaa tcaagaatc 
      tgattgaag catatgtga aaaaagtga gacatagta 
      aaaaggtga cctccagta gtagttccg tcaatcccc 
      agaaagctt aatgaacga ctcttgtgt acatattat 
      tttaagtta gtgattatt ttttaaaag tagactaaa 
      gaaataagt taatttatg tgaatcagc tgaatgaat 
      cttgtggac tttttcaat gtattgaaa ttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY