| General information | |||
| TC005009 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 662188..685552 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC005009 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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| Publish name | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_07815 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
previtellogenic egg chamber stage sequence irregular - Number of animals showing the phenotype: 2
mature egg not present - Number of animals showing the phenotype: 2
abdomen musculature random rounded - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC005009 RA
MSDDSDFEP RNKRRRNIR CLNDSGSSS ASSSVVTTT RKGIRRRFV EDSSDSDSE SSIEARPKR RVIPRVTSD PESDSSGSV IHRPKRTLR VVSDSSDDS DHGFEGSSS SQWETDVSD VETPAKIQD TGVDSDSSD GQSEKCPIC LISFTNQEI GTPESCDHL FCVDCIQEW SKNVNTCPV DRQEFRLIL VRKNLNGSI YREIQVQPP APQNEVDIL EDPTFCEIC GSSDREERM LLCDGCDLG FHMECLTPP LADIPPGAW FCNDCTPED LVDAEIELY ELQLLLDDA NEVGRPRSS RRTTTTSRL VPRTRQSER VRQRIANNR NNNEASDER PQPVSSTSR PVRRVKKKK RRKKKPRYR VVYQIDSTT GETIAVKKR RKKRRTKPV PKKTTVKKR LAQQLGICA PRNVPQNLP DVRTQPQTN SLQTQRYHA GIPTLNIFG NRDELDFVV SEEDDDFGD GGVVLRRGI TRRRTRIKS ITVPNSVSS STDVLGSIL DVQSRFHSK HTKFSLGQD GKFTMENTK DRLKDLNNW NQVVRQRPI SQETSDQPT SSGHRSSSP EHNNTAPTS TVENSNVSA ISSGNIQEQ YNNPQDYTQ SNLNTIYDV TMEEKRVDK KKKEDASED NTPRSGTPA SESEIDIYS DIETVTTSK VDEADFKAP SPLPATPNT GTDDDNNDS EPDMVIDTD KPEETTVTS TVDSNSEIT PTAQDVSSS SEIQSQAIQ SSNLLFDDK KYEEEKCVD DDDSEDGCP NFSIYSKET ITMAKTITE DAKPEENLE NDQVGEVSS SPATKTKEP VKKSTGLYS DSEDESIVK KENSFGITD LRNMTEDIS EEERSYTPC LDERPHHCI EGLDTEMIS DEDRNDFDE SHDLKTASD GDALEINAK ESELDFTRP EDYEEGEIV DKSKVKKET KEPVKKKDE TDKSGNKEN ENQNVKQPE FKKLSKSNK ERNYRDKDR ERSKDKDSK KDKKKEKRK EIERYDVRA LIAEKPRRL ITKDKFGRD VRRSPSGSV TKTPPRRTK SPSPKVRRS VSKARSTSR ERSRKKARK RRSVSRSKS RSRSKTKKR RRSASRERR RKSRSKGKS KKHRSRSRH RTRSPTPKR TKEWSRRHS RDWTPSLSR SRTPEPRHL TPSWTPPRI MDNTVKPHN LTVILTNDA NKKKKEKKR KSEKKTKEN ERAKKRKRN ERTPPPSKE VFASGDNIL VSVSFNKDN ETRDVSTKK KREVVEEVK KVKKDKTKK KKKNNRDLA GVKPVAIID LERSPFKEL TPSPKDIIV LSDSDNEEA RNKLQKTIC DSSQQVTSP VNNYTTGPK TPPEPQVKF TLTSKQTQM RAISNPLHD PEDIEPTGD ETQENVEHK GPNTPPEPP NSPPSSPDA YDPFEPTKS RSPTPEVAP SSNALNIDN NRDESITII STEVEKTDQ TPDQKSHTP PLADVQPAD SQSSIQVTP ESSLSKSPE RPVTNATQP VAQTIPFSS SVPTSLMTS TPVNSLAPP RINILSTTL LAPVSIPQR NVLPNANAT SKPARKSSN RNGSDVADT NLGFDSPYS PGSSDYEDL FEPPVETAP KTVTKTTKN VKSPSKVQN AFDALFGTS PFNKVKKQD SKKFSKKAP PAAVPSGGK GTKQVGVKI DEDNLKILD DLPNSAVEM QVKDKFLKK LNRQERVVE EVKLVLKPH YNKKHINKE EYKDILRRA VPKICHNKT GEINPTKIK NLIEAYVKK VRHSKKVTS SSSSVNPQK A
- >TC005009 RA
atgagtgat gatagtgat ttcgaacct cgaaataag cgcagacga aatattcgt tgtttaaac gacagtgga agcagttct gcaagtagt tcagtagtt acaacaaca cgaaaaggc atcagacga cgatttgtt gaagacagc tcggactca gacagtgag agttcgatt gaagcccgc cctaaacga agagttatt cctagagta acaagtgac ccagaatcg gacagttca ggctctgtt atccatcgt cctaaacgg acgcttcga gtggtcagc gattcgagt gacgactcg gaccatggc tttgaaggc agttcgagc agccaatgg gaaacagac gtttcagac gtcgaaact ccagctaaa attcaagac actggagtc gattccgac tcatccgat ggccaatca gaaaaatgc ccaatttgt ctcatctcg ttcaccaac caagaaatc ggaacgcct gaatcatgc gaccacttg ttctgcgtt gattgcatc caagagtgg tccaaaaac gtcaacact tgcccagtg gatagacaa gagtttcgc ctcatttta gtgcgaaaa aatttaaac gggagcatt tatcgcgaa atacaagtg caaccaccg gctccacag aatgaggtt gacattctt gaagatccg actttttgc gagatctgt ggttcgagt gatagggaa gagaggatg cttctgtgt gatggatgc gacttgggg tttcatatg gagtgtttg acgccacct cttgcggat attccacca ggtgcatgg ttttgtaat gattgtaca ccggaggat ttggttgat gctgaaatc gagttgtat gagcttcag ttgcttcta gatgatgct aatgaagtt gggaggccg agaagtagc cgcaggacg acaacaaca tcgagatta gtacctcgg actcgccaa agtgaaagg gttcgccaa cgaatagca aataaccga aacaacaat gaagcatct gatgaacgc cctcaacct gtttcaagt acatcacgt cctgtccga agagtaaag aagaagaaa cggaggaag aagaagcca cgttatcgt gtcgtttat cagatagat agtaccact ggtgaaaca atcgctgtg aaaaaacgc cgaaagaaa cgacgaaca aaaccagtc ccaaaaaaa accactgta aaaaaacga ttagcccaa caattgggt atttgtgca ccgagaaac gtcccacag aacttaccc gacgtgaga acacaacca caaacaaac agcttacaa acacaacgc taccatgca ggaatacca acgttaaat atttttgga aatcgggac gaattggat tttgtggta tctgaagaa gatgatgat tttggtgat ggtggtgtt gtgcttaga cgtggaata acacgtcga agaacacgt attaaaagt ataactgtt ccgaattcg gtttcaagc agtaccgat gttttgggg agcatttta gacgtccaa tcacgattc cattcgaaa catacgaag ttttccctt ggtcaggat ggtaaattt acgatggaa aacacaaaa gatagattg aaggattta aataattgg aatcaagtc gtaaggcaa cgaccgatt tcgcaggaa actagcgac caacctaca tcaagtggg cacaggagt tccagtcct gagcataac aacacagct ccgacatct accgttgaa aatagcaac gtttccgcc atttcgtct ggaaacatc caggaacag tacaataac ccacaggac tacacacag agcaatctc aatactatt tatgatgtg acaatggag gagaaaaga gtagataag aaaaaaaag gaagatgca tctgaagac aatacacca aggagtggg acaccggca agtgaatcg gaaattgac atttacagt gatattgaa actgtgact actagtaaa gttgacgaa gctgatttc aaagcacca tcacctcta cctgcaaca cctaacacc ggaaccgat gatgataat aatgatagt gaaccagac atggttata gatacggat aaacctgaa gaaacaaca gttacctca acggttgac tctaactca gaaataaca ccaacggca caagacgta tcttcatcg tccgaaatc cagtcacaa gcaatccag tcgtcgaac ctacttttc gacgataaa aaatacgaa gaagagaag tgtgttgat gatgatgat tcggaagat ggatgtcct aattttagt atatattcc aaagaaaca ataactatg gctaaaact ataacagag gacgcaaaa ccggaggaa aacctcgag aatgatcaa gtgggtgaa gtgagttct tcaccggcc accaaaaca aaagaacct gtaaaaaaa agtacaggt ctatatagt gattctgag gatgagtca atagttaag aaggaaaat tcgtttgga atcaccgat ttacgcaac atgactgaa gacatcagt gaggaggaa cgcagttat acgccgtgt cttgacgaa agaccgcat cattgtatc gaaggtttg gatacggaa atgatatca gatgaagac cggaacgat tttgatgaa tctcacgat ttaaaaacg gcctctgac ggcgatgcg cttgaaatc aatgcgaaa gaatcggaa ctagatttt acccgacct gaggattac gaagaaggt gaaatcgtc gataaatca aaggttaag aaagaaaca aaagaaccg gtgaaaaag aaggacgaa acggacaaa tccggaaat aaggaaaac gaaaatcag aatgtcaaa caacctgag tttaaaaaa ctgagtaaa agtaacaag gagcgcaac tatcgcgat aaagaccgc gaacggagt aaggacaaa gactcgaaa aaagataaa aagaaggag aaacgtaag gaaatcgag cggtatgat gttagggcg ttaatagcc gagaagccg cgacgtttg attacgaaa gataagttt gggcgtgat gttagaagg agtccgtcg gggtctgtg acgaagacg ccgcctaga cgtacgaag tcgccgtcg cctaaagtt cgtcggtcc gtttctaaa gcgaggtct acgtcgcgt gaaagaagt cgcaagaag gcgaggaaa cggcggtcg gtgtcgagg tccaagtca cggtcgagg tctaaaacg aagaagaga cggcggtcg gcaagcagg gagaggagg aggaaatca cgatcgaaa ggtaaaagc aaaaaacac cgaagtcgc agtagacac cgtaccagg tctcccaca cccaaacga acaaaagaa tggtctcga agacattca agagattgg acaccttca ttatcacga tcgcgaact cctgaaccg agacactta accccatca tggacaccc ccaagaatt atggataat accgtaaaa ccacacaac ttaactgtg attttaacc aacgatgcc aataagaaa aagaaggaa aagaaacgc aaatctgag aaaaagaca aaggaaaat gaacgtgca aaaaaacgg aaacgtaac gaacgtaca ccgccacct tccaaagaa gtgtttgct tcaggcgat aatattcta gttagtgtg agtttcaac aaagataac gaaacacga gacgtttcg acgaagaag aaacgcgaa gttgtcgaa gaagttaag aaggttaaa aaagacaaa acgaagaag aaaaagaag aataatcgt gatttggct ggagttaaa ccggtggcg attattgat ttggaaaga tcaccgttt aaagaattg acaccttca ccgaaagac attatcgtg ttgagcgat agtgataat gaagaagct aggaacaag ttacagaaa acaatttgt gattcgtca caacaagta accagtccg gttaataat tacacaact ggtcccaaa acaccacca gaacctcaa gttaagttc actttaacc tccaaacaa acacagatg cgtgcaatt tcgaatccg ttacatgac cctgaagat atcgaacca acgggtgac gaaacccag gaaaatgtg gaacataaa ggtcccaac acacctcca gaacctcca aattcacct ccaagttct ccagatgcc tacgatccg ttcgaacca acaaaatca cgatcacca acacctgaa gtagctcca agcagcaat gcgttaaat attgacaat aatcgtgac gaaagtatt accattatt agtacagag gtggaaaaa acggatcaa acgcctgat caaaaatca catacgcca cctttggcc gatgtacaa ccagccgac tcgcagagt agcatccaa gtcactccc gaatcaagc ttgagtaaa tcccccgaa cgtcccgta acaaatgcc acacaacca gttgctcaa acaattccg ttttcttcg tcagtaccg acgtcgtta atgacttcg acacctgtt aatagttta gcacctcca aggattaat attttgagt acgaccctt ttggcgccg gtgtccatc ccgcaaagg aatgttttg ccaaatgcc aatgcaaca tcgaaacct gcaaggaag tcgagtaat cggaacgga agtgatgtt gctgatacg aatttaggg tttgattcg ccctattcg ccaggatct agtgattat gaggatttg tttgaaccg ccagttgaa acagcaccg aagactgtg acgaaaacg acgaaaaat gttaaatca ccgtcaaag gtgcagaat gcgtttgat gcacttttt ggaacatcg ccgtttaat aaagttaag aaacaggac agtaagaag tttagcaag aaggcgccg cctgctgct gttccgagt gggggaaaa ggtactaaa caagtcgga gttaaaata gatgaagac aacttgaag attctcgat gatctgccg aattccgcc gtcgaaatg caagtaaag gacaagttt ttgaagaaa ctgaaccga caagagcga gtcgttgaa gaggttaaa ttggtctta aagccgcat tataacaag aaacatata aataaggag gagtacaag gatatctta agacgggct gttccaaag atttgtcac aacaaaacc ggcgaaata aatccgaca aaaatcaag aatctgatt gaagcatat gtgaaaaaa gtgagacat agtaaaaag gtgacctcc agtagtagt tccgtcaat ccccagaaa gcttaa
- >TC005009 RA
ctacggtac agtggctac gactttttg ctttttctg tcattggaa atcttgttt gttatgagt agaaacagt ttaaactga aattcgcta aaataaatc gatagataa gttaatctg gaaatctgt aagtaattg aaaccggtc gcttcacct tattatgta ttattgtaa cttggattt gaaaaatga gtgatgata gtgatttcg aacctcgaa ataagcgca gacgaaata ttcgttgtt taaacgaca gtggaagca gttctgcaa gtagttcag tagttacaa caacacgaa aaggcatca gacgacgat ttgttgaag acagctcgg actcagaca gtgagagtt cgattgaag cccgcccta aacgaagag ttattccta gagtaacaa gtgacccag aatcggaca gttcaggct ctgttatcc atcgtccta aacggacgc ttcgagtgg tcagcgatt cgagtgacg actcggacc atggctttg aaggcagtt cgagcagcc aatgggaaa cagacgttt cagacgtcg aaactccag ctaaaattc aagacactg gagtcgatt ccgactcat ccgatggcc aatcagaaa aatgcccaa tttgtctca tctcgttca ccaaccaag aaatcggaa cgcctgaat catgcgacc acttgttct gcgttgatt gcatccaag agtggtcca aaaacgtca acacttgcc cagtggata gacaagagt ttcgcctca ttttagtgc gaaaaaatt taaacggga gcatttatc gcgaaatac aagtgcaac caccggctc cacagaatg aggttgaca ttcttgaag atccgactt tttgcgaga tctgtggtt cgagtgata gggaagaga ggatgcttc tgtgtgatg gatgcgact tggggtttc atatggagt gtttgacgc cacctcttg cggatattc caccaggtg catggtttt gtaatgatt gtacaccgg aggatttgg ttgatgctg aaatcgagt tgtatgagc ttcagttgc ttctagatg atgctaatg aagttggga ggccgagaa gtagccgca ggacgacaa caacatcga gattagtac ctcggactc gccaaagtg aaagggttc gccaacgaa tagcaaata accgaaaca acaatgaag catctgatg aacgccctc aacctgttt caagtacat cacgtcctg tccgaagag taaagaaga agaaacgga ggaagaaga agccacgtt atcgtgtcg tttatcaga tagatagta ccactggtg aaacaatcg ctgtgaaaa aacgccgaa agaaacgac gaacaaaac cagtcccaa aaaaaacca ctgtaaaaa aacgattag cccaacaat tgggtattt gtgcaccga gaaacgtcc cacagaact tacccgacg tgagaacac aaccacaaa caaacagct tacaaacac aacgctacc atgcaggaa taccaacgt taaatattt ttggaaatc gggacgaat tggattttg tggtatctg aagaagatg atgattttg gtgatggtg gtgttgtgc ttagacgtg gaataacac gtcgaagaa cacgtatta aaagtataa ctgttccga attcggttt caagcagta ccgatgttt tggggagca ttttagacg tccaatcac gattccatt cgaaacata cgaagtttt cccttggtc aggatggta aatttacga tggaaaaca caaaagata gattgaagg atttaaata attggaatc aagtcgtaa ggcaacgac cgatttcgc aggaaacta gcgaccaac ctacatcaa gtgggcaca ggagttcca gtcctgagc ataacaaca cagctccga catctaccg ttgaaaata gcaacgttt ccgccattt cgtctggaa acatccagg aacagtaca ataacccac aggactaca cacagagca atctcaata ctatttatg atgtgacaa tggaggaga aaagagtag ataagaaaa aaaaggaag atgcatctg aagacaata caccaagga gtgggacac cggcaagtg aatcggaaa ttgacattt acagtgata ttgaaactg tgactacta gtaaagttg acgaagctg atttcaaag caccatcac ctctacctg caacaccta acaccggaa ccgatgatg ataataatg atagtgaac cagacatgg ttatagata cggataaac ctgaagaaa caacagtta cctcaacgg ttgactcta actcagaaa taacaccaa cggcacaag acgtatctt catcgtccg aaatccagt cacaagcaa tccagtcgt cgaacctac ttttcgacg ataaaaaat acgaagaag agaagtgtg ttgatgatg atgattcgg aagatggat gtcctaatt ttagtatat attccaaag aaacaataa ctatggcta aaactataa cagaggacg caaaaccgg aggaaaacc tcgagaatg atcaagtgg gtgaagtga gttcttcac cggccacca aaacaaaag aacctgtaa aaaaaagta caggtctat atagtgatt ctgaggatg agtcaatag ttaagaagg aaaattcgt ttggaatca ccgatttac gcaacatga ctgaagaca tcagtgagg aggaacgca gttatacgc cgtgtcttg acgaaagac cgcatcatt gtatcgaag gtttggata cggaaatga tatcagatg aagaccgga acgattttg atgaatctc acgatttaa aaacggcct ctgacggcg atgcgcttg aaatcaatg cgaaagaat cggaactag attttaccc gacctgagg attacgaag aaggtgaaa tcgtcgata aatcaaagg ttaagaaag aaacaaaag aaccggtga aaaagaagg acgaaacgg acaaatccg gaaataagg aaaacgaaa atcagaatg tcaaacaac ctgagttta aaaaactga gtaaaagta acaaggagc gcaactatc gcgataaag accgcgaac ggagtaagg acaaagact cgaaaaaag ataaaaaga aggagaaac gtaaggaaa tcgagcggt atgatgtta gggcgttaa tagccgaga agccgcgac gtttgatta cgaaagata agtttgggc gtgatgtta gaaggagtc cgtcggggt ctgtgacga agacgccgc ctagacgta cgaagtcgc cgtcgccta aagttcgtc ggtccgttt ctaaagcga ggtctacgt cgcgtgaaa gaagtcgca agaaggcga ggaaacggc ggtcggtgt cgaggtcca agtcacggt cgaggtcta aaacgaaga agagacggc ggtcggcaa gcagggaga ggaggagga aatcacgat cgaaaggta aaagcaaaa aacaccgaa gtcgcagta gacaccgta ccaggtctc ccacaccca aacgaacaa aagaatggt ctcgaagac attcaagag attggacac cttcattat cacgatcgc gaactcctg aaccgagac acttaaccc catcatgga cacccccaa gaattatgg ataataccg taaaaccac acaacttaa ctgtgattt taaccaacg atgccaata agaaaaaga aggaaaaga aacgcaaat ctgagaaaa agacaaagg aaaatgaac gtgcaaaaa aacggaaac gtaacgaac gtacaccgc caccttcca aagaagtgt ttgcttcag gcgataata ttctagtta gtgtgagtt tcaacaaag ataacgaaa cacgagacg tttcgacga agaagaaac gcgaagttg tcgaagaag ttaagaagg ttaaaaaag acaaaacga agaagaaaa agaagaata atcgtgatt tggctggag ttaaaccgg tggcgatta ttgatttgg aaagatcac cgtttaaag aattgacac cttcaccga aagacatta tcgtgttga gcgatagtg ataatgaag aagctagga acaagttac agaaaacaa tttgtgatt cgtcacaac aagtaacca gtccggtta ataattaca caactggtc ccaaaacac caccagaac ctcaagtta agttcactt taacctcca aacaaacac agatgcgtg caatttcga atccgttac atgaccctg aagatatcg aaccaacgg gtgacgaaa cccaggaaa atgtggaac ataaaggtc ccaacacac ctccagaac ctccaaatt cacctccaa gttctccag atgcctacg atccgttcg aaccaacaa aatcacgat caccaacac ctgaagtag ctccaagca gcaatgcgt taaatattg acaataatc gtgacgaaa gtattacca ttattagta cagaggtgg aaaaaacgg atcaaacgc ctgatcaaa aatcacata cgccacctt tggccgatg tacaaccag ccgactcgc agagtagca tccaagtca ctcccgaat caagcttga gtaaatccc ccgaacgtc ccgtaacaa atgccacac aaccagttg ctcaaacaa ttccgtttt cttcgtcag taccgacgt cgttaatga cttcgacac ctgttaata gtttagcac ctccaagga ttaatattt tgagtacga cccttttgg cgccggtgt ccatcccgc aaaggaatg ttttgccaa atgccaatg caacatcga aacctgcaa ggaagtcga gtaatcgga acggaagtg atgttgctg atacgaatt tagggtttg attcgccct attcgccag gatctagtg attatgagg atttgtttg aaccgccag ttgaaacag caccgaaga ctgtgacga aaacgacga aaaatgtta aatcaccgt caaaggtgc agaatgcgt ttgatgcac tttttggaa catcgccgt ttaataaag ttaagaaac aggacagta agaagttta gcaagaagg cgccgcctg ctgctgttc cgagtgggg gaaaaggta ctaaacaag tcggagtta aaatagatg aagacaact tgaagattc tcgatgatc tgccgaatt ccgccgtcg aaatgcaag taaaggaca agtttttga agaaactga accgacaag agcgagtcg ttgaagagg ttaaattgg tcttaaagc cgcattata acaagaaac atataaata aggaggagt acaaggata tcttaagac gggctgttc caaagattt gtcacaaca aaaccggcg aaataaatc cgacaaaaa tcaagaatc tgattgaag catatgtga aaaaagtga gacatagta aaaaggtga cctccagta gtagttccg tcaatcccc agaaagctt aatgaacga ctcttgtgt acatattat tttaagtta gtgattatt ttttaaaag tagactaaa gaaataagt taatttatg tgaatcagc tgaatgaat cttgtggac tttttcaat gtattgaaa ttt
