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Gene information
General information
TC030553 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 2557473..2565576
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030553
    iBeetle screen iB_07800

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 0 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030553 RA
      MPHQVIDRS EKFSVARRR RKEGRPRRQ 
      RTTFSSEQT LRLEIEYQR SEYISRGRR 
      CELAEALKL SETQIKIWF QNRRAKDKR 
      IEKAHIDHH YRKLLGAFP GALCPICLD 
      GPCFHVSPV LQTQFPGLQ SANTDNAPL 
      YKKSESSQG VAEEIDIL
    CDS sequence
    • >TC030553 RA
      atgccccat caggtaatt gaccgtagc gagaaattt 
      tcagtagcg agacggagg cgcaaagag ggaaggccc 
      aggaggcag aggaccact ttcagcagc gaacagaca 
      ctgaggctt gagattgag taccaaagg tccgaatat 
      atcagcaga ggacgaaga tgcgaactg gcagaagcg 
      ctcaagctt tcggagact cagattaag atctggttt 
      cagaatcga agggcgaag gacaagagg attgagaag 
      gcgcatata gaccatcac tacaggaaa ttattgggc 
      gcctttccc ggtgccctc tgtccaata tgtttagac 
      ggcccatgt ttccacgta tcaccagtg ttgcagacg 
      caatttcca ggcctgcaa tccgcaaat accgataat 
      gctcccctt tacaaaaaa agcgaaagt agccaaggt 
      gtcgctgaa gaaatcgat attttgtaa
    mRNA sequence
    • >TC030553 RA
      ctttcagag ggtgagtgg agtctagaa ataaaacaa 
      actcaaaga aattaaaat tacatttag ttgagccat 
      ttgttggaa ttagagaaa aatgtgata aaaatttgc 
      atgtgtcca catgtgcat ataactaaa tttttatta 
      aattttaaa attaaagag attattgtt ttaataatt 
      ttcaaattc tgtgaaata ttctaagta cgaaaaaac 
      attacttac gtttatttt cgctgcatt cattaattt 
      attaattat ttaaaaaca tttataata aaataataa 
      cacctttaa attaatcac aacgtaggt atacactcg 
      ttcatagac aagttgcta ttgcgctgg tagcaaaat 
      aaattgatt aatgataat aatacacca tatgtgcat 
      tatttacac attcatcag aataccgca acacttgag 
      tggcgtagc atcccgaat ttatcctgg aaatgtgtc 
      ccgatttga atgccgcta atgcgctta caagaggct 
      ttaatgttg cgataacaa tgcccgtgc aatatctat 
      ttttcgttc gacaaatcc ctctaaagt ttatgttgc 
      ggtgggtgc gaattctcc agcttagag acgagtttc 
      tttgcggcg aaattattt ttaccggga agtggggct 
      cgtgtgaac ctcaacagg acgacatat gccgtgatt 
      gacggaaat gcgaagccc catctgacg ccatatgcc 
      ccatcaggt aattgaccg tagcgagaa attttcagt 
      agcgagacg gaggcgcaa agagggaag gcccaggag 
      gcagaggac cactttcag cagcgaaca gacactgag 
      gcttgagat tgagtacca aaggtccga atatatcag 
      cagaggacg aagatgcga actggcaga agcgctcaa 
      gctttcgga gactcagat taagatctg gtttcagaa 
      tcgaagggc gaaggacaa gaggattga gaaggcgca 
      tatagacca tcactacag gaaattatt gggcgcctt 
      tcccggtgc cctctgtcc aatatgttt agacggccc 
      atgtttcca cgtatcacc agtgttgca gacgcaatt 
      tccaggcct gcaatccgc aaataccga taatgctcc 
      cctttacaa aaaaagcga aagtagcca aggtgtcgc 
      tgaagaaat cgatatttt gtaatgtgt ccgtttatt 
      ttgtccttc tagtcaccg ttttaatgt acataaata 
      aataaaaat tgagaaacg ataagttag gtcttttat 
      tgcggttat cgctatata aatatcgtg ctcaacaat 
      catcgaggt aaacggagc gtgccgccg attcacaaa 
      agaccagat tacctcacc aggtggagg ttcccgcgc 
      atcatttta tccctttaa aaattgacc ctcacttaa 
      tttctattt tctcgttgc gttttccac aaaataata 
      aaagtttgc aacaataga aatgctgat gaagaaaac 
      caatcaagt gtgtgttac gcacgaacg agccgattg 
      gaattattt ctccaaggg aagggacgg aaataaaac 
      acttgaaat tgctaataa taatttatt cgtctgaaa 
      cactcactc gacattcac actgcttca atactcacc 
      gctgccggc agtcaccga acgaaactg gacccccag 
      aaaacaaaa tgaattaat atcgtcata aaaacggga 
      caggtgcgt gcacggact aattaggag acgaaacga 
      aatacaaaa tttacactg tttggaaga aatgcggaa 
      gcgttga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY