Status
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Gene information
General information
TC004523 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 3252604..3255767
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004523
    iBeetle screen iB_07796

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larva partially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004523 RA
      MCNATLIQV ICLFLAIQI VPSFSFHQS NITRREAKE 
      CPRNSFACK SGECIDEDK ECDGGVDCK DASDESNAC 
      ARIKCPISA FRCDYGACI SADLECDGK PDCRDGSDE 
      KTPNCQIID ETSPICKSN EFRCSSGEC IDEDNKCDG 
      IAQCSDRSD EIRATCWNL RCPIYSYKC KYGACVSGN 
      AECNGKIEC QDGSDEDPN ICKNSTVLT PTPSPVVPR 
      PGARGRCVL PNHPEFGKW SIFGPENLS PGATVNPGT 
      ILNIVCQNG YKLEGNSVI YCANGQWTE NIGVCLKLC 
      SPLSSTKVM TVTCTYPNN KGEGNCTNA IDGTLARFK 
      CEPLYEDPN LQRIPGRVC RDGTWDNSS PECVPVCGQ 
      KSVEVQKLI VNGKTAKRG TYPWQAALY TRDKKELIC 
      GGSLIKLNM IITAAHCVT DQQERAQPL PKENYIVAL 
      GKYYRKFDD PRDSKEAQF SELKKIIVN EKYSGPIQN 
      FGSDIALLI TSTVFVPSL RVQPVCMDW NLQFKIGDD 
      QVFGYVTGW GYTVEGSNP SEELKELKV PLIPESKCR 
      KDLPVDYDR YYTYDKLCA GYLNSNTSV CRGDSGGGL 
      VVKRNGDRF YLTGIVSLS PTSPREIDG CDSQQYGLY 
      TKVAAHTED FILKKVTEY GINAR
    CDS sequence
    • >TC004523 RA
      atgtgcaat gcaacacta atccaagta atttgttta 
      ttccttgcc atccaaata gtgcccagc ttcagtttt 
      catcaaagt aatatcaca agacgcgaa gccaaggaa 
      tgtccaagg aacagtttc gcgtgcaaa tccggcgaa 
      tgcatcgac gaggacaag gaatgcgac ggcggcgtc 
      gactgcaaa gacgccagt gacgaaagc aacgcctgt 
      gccaggatc aagtgtcca atttcggcg tttcgctgc 
      gactacggt gcgtgcatt tctgcggac ctggagtgc 
      gacgggaaa cccgactgt cgagacggc tccgacgaa 
      aagaccccc aactgccaa atcatcgac gaaacgtcc 
      ccaatttgc aagagcaac gaatttcga tgcagttct 
      ggcgaatgc atcgacgag gataacaaa tgcgacgga 
      attgcacag tgttcggac cggtcggac gaaatcaga 
      gccacttgt tggaacctg cggtgtccg atttacagc 
      tacaagtgc aaatatggg gcttgtgtt agcgggaac 
      gcggaatgt aacggcaag attgagtgc caggatggg 
      tccgatgag gaccccaat atttgcaaa aacagcaca 
      gtgctgacc ccgacccca tcacccgtg gtaccccga 
      cctggagcg aggggtcgg tgcgttttg ccgaaccat 
      cccgagttc ggaaaatgg agtattttc gggccggaa 
      aacttgtcc cctggggcc acggtgaat ccagggacg 
      attttaaat atagtctgc caaaatggc tacaaactg 
      gaagggaat tcggtgatt tattgcgcg aatgggcaa 
      tggaccgag aatattgga gtttgtcta aaattgtgt 
      tcacctttg tcaagtacc aaagtcatg acggtcact 
      tgcacgtat cccaataat aaaggagaa gggaactgt 
      accaatgca attgatggt actcttgca aggttcaag 
      tgtgaacca ctctacgaa gatccaaac ttgcagaga 
      attccagga cgggtttgt cgcgatggt acctgggac 
      aacagctca cctgagtgt gtaccagtt tgtgggcag 
      aaatcggtc gaggtgcag aaactgatc gtcaacggc 
      aagacagcg aaacgaggg acgtacccg tggcaagcg 
      gctttgtac acccgcgac aagaaggag ttaatttgt 
      ggcggatct ttgataaaa ctgaatatg ataatcaca 
      gctgcgcat tgtgtaacc gatcaacag gaacgagcg 
      cagcctctt cccaaagaa aactacatc gttgcgttg 
      gggaaatac tacaggaaa tttgatgat ccacgcgat 
      tccaaagaa gcgcaattt tcggagctg aaaaaaata 
      attgtcaat gagaaatac agtggtccg atacaaaat 
      ttcggttcg gatatcgct ctcctaatc actagcact 
      gtttttgtg ccgtcacta agagtacaa cctgtttgc 
      atggactgg aatcttcaa ttcaaaata ggggacgac 
      caagttttc ggttatgtg actggttgg gggtataca 
      gttgagggc tcgaatccg tccgaggag ctgaaggag 
      ctgaaagtt ccccttatt cctgaatcc aagtgtcgg 
      aaagactta ccagttgat tacgacagg tactacact 
      tacgataag ttgtgtgcc ggctacctc aactcaaat 
      acttctgtt tgtcggggc gacagtggc ggaggttta 
      gtcgtaaaa cgcaacggt gaccgattt tacctcact 
      ggaattgta agtctttcc ccgacttca ccacgggag 
      atcgacggc tgtgatagt caacagtac ggactttat 
      acgaaagtt gcagctcat acggaggac tttattttg 
      aaaaaagtg acagaatat ggaataaat gcgcgctaa
    mRNA sequence
    • >TC004523 RA
      gccaaatta aataacgaa cattttttg ttgtcagaa 
      atctaaatt gaaattttg caattaaaa aaattatta 
      ttatgatgt catactttt ttgggacac tcaatataa 
      tcaaaaata ttttcctga aatacgtcc taaagtaca 
      aataacgtc tgcaaaata tcaaaagtc caactatac 
      taataacag ccacagaaa acgtgagct gagctgggc 
      cacactgta gattttagt caagtcgta attgagcaa 
      gcgaacaac tcaacaaca aaatttaca aattacacg 
      tttccgagt agctgcgat acgagggga attccgttt 
      acacagcac cacaagaaa gattgagca gaaacgagt 
      tgtcttcgc gataaacca atgcgacct ccattccgg 
      gtcatccta agtgtaaaa ttgtttatc caatacaca 
      atcatgttg aaatagtag tgaccacaa tacaaaact 
      gtgcatttg caattactt tgtttatga aaatgtgca 
      atgcaacac taatccaag taatttgtt tattccttg 
      ccatccaaa tagtgccca gcttcagtt ttcatcaaa 
      gtaatatca caagacgcg aagccaagg aatgtccaa 
      ggaacagtt tcgcgtgca aatccggcg aatgcatcg 
      acgaggaca aggaatgcg acggcggcg tcgactgca 
      aagacgcca gtgacgaaa gcaacgcct gtgccagga 
      tcaagtgtc caatttcgg cgtttcgct gcgactacg 
      gtgcgtgca tttctgcgg acctggagt gcgacggga 
      aacccgact gtcgagacg gctccgacg aaaagaccc 
      ccaactgcc aaatcatcg acgaaacgt ccccaattt 
      gcaagagca acgaatttc gatgcagtt ctggcgaat 
      gcatcgacg aggataaca aatgcgacg gaattgcac 
      agtgttcgg accggtcgg acgaaatca gagccactt 
      gttggaacc tgcggtgtc cgatttaca gctacaagt 
      gcaaatatg gggcttgtg ttagcggga acgcggaat 
      gtaacggca agattgagt gccaggatg ggtccgatg 
      aggacccca atatttgca aaaacagca cagtgctga 
      ccccgaccc catcacccg tggtacccc gacctggag 
      cgaggggtc ggtgcgttt tgccgaacc atcccgagt 
      tcggaaaat ggagtattt tcgggccgg aaaacttgt 
      cccctgggg ccacggtga atccaggga cgattttaa 
      atatagtct gccaaaatg gctacaaac tggaaggga 
      attcggtga tttattgcg cgaatgggc aatggaccg 
      agaatattg gagtttgtc taaaattgt gttcacctt 
      tgtcaagta ccaaagtca tgacggtca cttgcacgt 
      atcccaata ataaaggag aagggaact gtaccaatg 
      caattgatg gtactcttg caaggttca agtgtgaac 
      cactctacg aagatccaa acttgcaga gaattccag 
      gacgggttt gtcgcgatg gtacctggg acaacagct 
      cacctgagt gtgtaccag tttgtgggc agaaatcgg 
      tcgaggtgc agaaactga tcgtcaacg gcaagacag 
      cgaaacgag ggacgtacc cgtggcaag cggctttgt 
      acacccgcg acaagaagg agttaattt gtggcggat 
      ctttgataa aactgaata tgataatca cagctgcgc 
      attgtgtaa ccgatcaac aggaacgag cgcagcctc 
      ttcccaaag aaaactaca tcgttgcgt tggggaaat 
      actacagga aatttgatg atccacgcg attccaaag 
      aagcgcaat tttcggagc tgaaaaaaa taattgtca 
      atgagaaat acagtggtc cgatacaaa atttcggtt 
      cggatatcg ctctcctaa tcactagca ctgtttttg 
      tgccgtcac taagagtac aacctgttt gcatggact 
      ggaatcttc aattcaaaa taggggacg accaagttt 
      tcggttatg tgactggtt gggggtata cagttgagg 
      gctcgaatc cgtccgagg agctgaagg agctgaaag 
      ttcccctta ttcctgaat ccaagtgtc ggaaagact 
      taccagttg attacgaca ggtactaca cttacgata 
      agttgtgtg ccggctacc tcaactcaa atacttctg 
      tttgtcggg gcgacagtg gcggaggtt tagtcgtaa 
      aacgcaacg gtgaccgat tttacctca ctggaattg 
      taagtcttt ccccgactt caccacggg agatcgacg 
      gctgtgata gtcaacagt acggacttt atacgaaag 
      ttgcagctc atacggagg actttattt tgaaaaaag 
      tgacagaat atggaataa atgcgcgct aaatgaaac 
      tttgtattt tttagtaat aaaatacca aacgtact
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY