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Gene information
General information
TC004849 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 3049299..3054086
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004849
    iBeetle screen iB_07795

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004849 RA
      MESSEAPSS GKDSSQCKV LIVGAGMAG LSAAYHLSK 
      NGFNDYKLL EARNRVGGR IVQIKMGSE PVELGANWI 
      HGVLGNPVY ELAMQHGLV DIMQTPKPH KVIAATENG 
      KQVPFATLH EIYEAYLCF LRRCEEYFL SQYLPPEGI 
      DSVGDHIKL EISLYLDKV QDPRDRHLR ELLFECLLK 
      RETCISGCD DMSEIDLLE LGTYTELQG GNITLPGGY 
      SSILGPVTQ AIPAENLLV GHPVSQIRW NLNKRNSID 
      NGNDSDDSD RTVIEETTK ESNSPNVEV HCDNGKVFK 
      ADQLICTIP LGVLKYNKD TLFQPPLPE YKREAIDRL 
      LFGTVDKIL LEYERPFLH PSITEVLLL WESDTEHPE 
      GQNDLSKNW YKKIYSFSK ITETIILGW ISGKEAEYM 
      ETLSKDEIK DTCTTVLRK FLNDPFIPK PKNVVCTSW 
      HSQPYTRGS YTAIAVGAS QIDIECLAQ PLFLDEEET 
      KPVVLFAGE HTHCNFYST VHGAYLTGR TAAQAVLHA 
      DAPQEMVLD CEDDADLSS WVQGICLE
    CDS sequence
    • >TC004849 RA
      atggaaagc tccgaagcc ccctcaagc ggaaaagac 
      tcgtcgcaa tgtaaagtg ttgatcgtg ggggcgggc 
      atggcgggg ctctccgcc gcctaccac ctctccaag 
      aacggtttc aacgactac aagctcctg gaggcccgc 
      aaccgggtc gggggccgt atcgtgcaa attaaaatg 
      gggagcgaa ccggtggaa ctgggggcc aactggatc 
      cacggagtg ctgggcaac cccgtgtac gaactggca 
      atgcagcac ggtctcgtc gacattatg caaacgccc 
      aaaccccat aaagtaatc gcggcgacc gaaaacggg 
      aagcaagtc ccttttgca accttgcac gaaatctac 
      gaagcatat ctgtgtttt ctgcggcgc tgcgaggag 
      tatttttta tcgcagtat ttgccccct gaaggcatc 
      gacagtgtg ggtgatcac attaagtta gagattagt 
      ttgtatctg gacaaggtc caggacccg cgtgataga 
      cacttgagg gaactgttg tttgagtgt ttgttgaaa 
      cgggagact tgtatcagt gggtgcgac gatatgagc 
      gaaattgac ttgctcgag ttggggacg tacacggag 
      ctgcaaggg gggaatatc acgcttcca ggggggtac 
      agctccatt ttggggccc gtgacgcaa gccattccc 
      gcggaaaac ctgctagtg gggcacccg gtgtcccaa 
      atccggtgg aatctcaac aagaggaac agtatcgac 
      aacgggaac gactcggat gattccgac cggactgtg 
      attgaggaa accacgaaa gaatcaaat tcgcccaac 
      gttgaagta cattgtgat aacgggaaa gtattcaaa 
      gccgaccaa ctcatctgc accattccg ttaggggtc 
      ctaaagtac aataaagac actcttttc caaccacct 
      ttacccgaa tataaacgc gaggcgatt gaccggttg 
      ctattcggg accgtagat aagatttta ctagagtac 
      gaaagaccg tttttacac cccagtatt accgaagtt 
      ttgctcttg tgggagtca gatactgag catcccgaa 
      ggtcagaat gacctgagc aaaaattgg tacaagaaa 
      atctactcg ttttccaaa atcactgaa actattatc 
      ctcggctgg atttcgggg aaagaagct gagtatatg 
      gagacgttg tcaaaagac gagattaaa gacacctgc 
      acgactgtt ttacggaag ttcctcaat gaccctttc 
      atacccaaa ccaaaaaat gtagtttgc acaagctgg 
      cacagtcag ccctatacc cgcggctcc tacactgcg 
      atagccgtg ggggcctca cagatcgac atcgagtgt 
      ctggcacaa cccctattc ctcgacgag gaagaaact 
      aaacctgtg gtattattc gctggcgaa catacccac 
      tgtaatttt tactcaaca gttcatggg gcttattta 
      actgggaga acagcggca caagcagtt ttacacgca 
      gatgccccc caggaaatg gtgctagat tgtgaagat 
      gatgccgat ttgagttcg tgggtgcaa ggaatctgt 
      cttgagtaa
    mRNA sequence
    • >TC004849 RA
      atggggctg caagtatgg aaagctccg aagccccct 
      caagcggaa aagactcgt cgcaatgta aagtgttga 
      tcgtggggg cgggcatgg cggggctct ccgccgcct 
      accacctct ccaagaacg gtttcaacg actacaagc 
      tcctggagg cccgcaacc gggtcgggg gccgtatcg 
      tgcaaatta aaatgggga gcgaaccgg tggaactgg 
      gggccaact ggatccacg gagtgctgg gcaaccccg 
      tgtacgaac tggcaatgc agcacggtc tcgtcgaca 
      ttatgcaaa cgcccaaac cccataaag taatcgcgg 
      cgaccgaaa acgggaagc aagtccctt ttgcaacct 
      tgcacgaaa tctacgaag catatctgt gttttctgc 
      ggcgctgcg aggagtatt ttttatcgc agtatttgc 
      cccctgaag gcatcgaca gtgtgggtg atcacatta 
      agttagaga ttagtttgt atctggaca aggtccagg 
      acccgcgtg atagacact tgagggaac tgttgtttg 
      agtgtttgt tgaaacggg agacttgta tcagtgggt 
      gcgacgata tgagcgaaa ttgacttgc tcgagttgg 
      ggacgtaca cggagctgc aagggggga atatcacgc 
      ttccagggg ggtacagct ccattttgg ggcccgtga 
      cgcaagcca ttcccgcgg aaaacctgc tagtggggc 
      acccggtgt cccaaatcc ggtggaatc tcaacaaga 
      ggaacagta tcgacaacg ggaacgact cggatgatt 
      ccgaccgga ctgtgattg aggaaacca cgaaagaat 
      caaattcgc ccaacgttg aagtacatt gtgataacg 
      ggaaagtat tcaaagccg accaactca tctgcacca 
      ttccgttag gggtcctaa agtacaata aagacactc 
      ttttccaac cacctttac ccgaatata aacgcgagg 
      cgattgacc ggttgctat tcgggaccg tagataaga 
      ttttactag agtacgaaa gaccgtttt tacacccca 
      gtattaccg aagttttgc tcttgtggg agtcagata 
      ctgagcatc ccgaaggtc agaatgacc tgagcaaaa 
      attggtaca agaaaatct actcgtttt ccaaaatca 
      ctgaaacta ttatcctcg gctggattt cggggaaag 
      aagctgagt atatggaga cgttgtcaa aagacgaga 
      ttaaagaca cctgcacga ctgttttac ggaagttcc 
      tcaatgacc ctttcatac ccaaaccaa aaaatgtag 
      tttgcacaa gctggcaca gtcagccct atacccgcg 
      gctcctaca ctgcgatag ccgtggggg cctcacaga 
      tcgacatcg agtgtctgg cacaacccc tattcctcg 
      acgaggaag aaactaaac ctgtggtat tattcgctg 
      gcgaacata cccactgta atttttact caacagttc 
      atggggctt atttaactg ggagaacag cggcacaag 
      cagttttac acgcagatg ccccccagg aaatggtgc 
      tagattgtg aagatgatg ccgatttga gttcgtggg 
      tgcaaggaa tctgtcttg agtaactgc cttaaatat 
      tgtgttttc gttaatttg tgaacgact agtagtcaa 
      ttttaggac cgcttaact ttttgttac cgtttaaac 
      tatcacggc ttcattggt caacgaaag ttgagttac 
      tatggttaa ctgtggtaa ttggtcaaa gaagctgtg 
      aaatcagtt gttactttt ataggcttt acatgatgt 
      atgctagtc attttttta aga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY