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Gene information
General information
TC011127 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 4254414..4258267
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011127
    iBeetle screen iB_07793

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011127 RA
      MNSGESESE SSNSGSEDK STIIHKILG EMKSEFNSP 
      RADDAPNTN DEEKPRIIL SFKKPASDE SPPVGGRRS 
      LRYKNPEEK PELKRSARR RSKDCSESV LQSAIARKE 
      KSYNESNKP QRLTRQLKP TQKILENLA LAKMEKSRF 
      FECEESERL VRKHKHKHN KCRRLKRAK LGQGDSEKE 
      TDSDEGGCR RPRSENVDE GEELEGAKD TCLCRRRTK 
      FFLATNEET AYCAAVDSI DDKLIGCGR TVATKDLML 
      LRPSTRVPY TIFCDLHKS RLLRHNCCP TCGIFCTQG 
      RFVQCENKH QYHKDCQSE VDCCPHCGI SGISYQVDI 
      VMQLGKKPV FLPVQKAQY ITARMTFST LKTEESKCM 
      TPPLIPIGS FEFDNSFEY GDEVYEMNE LIVAVKNNA 
      LEKIATILG SKTLDVNSK IPECQDGTI LHIAAEKGN 
      VSIVHMLVV AGTQTDVYD KDQNTPLMA AVMAANNET 
      VRYLIKTGA DIHLKGTDG MTALHLAAK CGNLHACQL 
      LVDNSPRKA VNCQDDGGW TPLVWACEH GYGDVATFL 
      INKGADPSV RDVEHNVAL HWAAFSGSC HIAGLLLDK 
      GCDVNAVNA HGDTPLHIG ARQDMYDCN VVLLARGAD 
      VTLLNKNNE SPLDCVPAG GDTYSVIAL NVTLGGAYN 
      SQNVVLCND ITKGRESNP IQCYNSVDN ATNPNDFKY 
      VTKNCITSD DVKIEAKIT DLQCCQCEE RCVTDDCQC 
      GKLSLRCWY DEEGKLIPE FNFGDIPMI FECNDRCQC 
      NAITCNNRV VQKGPNQRF ELFKTLDKG WGIRTLRPI 
      SRGSFICEY IGEIITDSE ADKREDDSF LFDLENRVC 
      KNEDVDSYC IDAKFYGNF ARFINHSCN PNLTSVKVF 
      IDHQDLRFP RIAFFANRD ISNEEELSF DYGEKFWLA 
      KYKLFSCLC GSLECKYSE DTIGMRT
    CDS sequence
    • >TC011127 RA
      atgaacagt ggtgaaagt gagtccgaa tcgagtaat 
      tccggcagt gaggacaag tcaacaatc atccacaaa 
      atccttggc gaaatgaaa agtgaattc aacagcccc 
      cgagctgac gacgccccc aataccaac gacgaagaa 
      aaaccccga attattctc tcctttaaa aaacctgct 
      tcggatgag tcgccccca gttgggggg cggcgctcc 
      ctccgttac aaaaacccg gaggaaaag cccgaattg 
      aaacgttct gcaaggagg cgcagtaaa gactgctcc 
      gagtcggtt ttacagagc gctattgca cgtaaagaa 
      aagtcctac aatgagtca aataagccc caaagactt 
      acaagacaa ttgaaaccg actcagaaa attttagaa 
      aatttagcg ttggctaaa atggaaaag agtcggttt 
      tttgaatgt gaggagagc gaaagactt gtaagaaaa 
      cacaaacat aagcataac aagtgtcgg aggctgaaa 
      agggccaaa ttggggcaa ggggacagc gagaaagag 
      actgattcg gatgaaggg ggctgtcgg aggccgagg 
      agcgaaaat gtggacgaa ggggaggag ttggagggg 
      gccaaagac acgtgcttg tgtcggaga aggacgaag 
      ttttttctc gcaacgaat gaagagacg gcctactgc 
      gccgccgtg gacagcatc gatgataaa ttgattggt 
      tgtggtcgc actgtagcc acgaaagac ttgatgttg 
      ttgcgacct tcaacccgt gttccatac acgattttt 
      tgcgatttg cataaaagt cgtctcttg cgtcataat 
      tgttgtcca acgtgtggt attttttgt acacaggga 
      cgtttcgtc caatgtgag aacaaacat cagtatcat 
      aaagactgt caaagtgaa gttgattgt tgtccacat 
      tgtggtatt tctggtatt tcgtatcaa gttgatatc 
      gtaatgcaa ttggggaaa aaaccggtt tttttgccc 
      gtacaaaaa gcgcaatat attacggca agaatgaca 
      ttttcgact ttaaaaaca gaagaatcc aaatgtatg 
      acgccacct ttgatccca atcggatcg tttgaattt 
      gacaattca ttcgaatat ggcgacgaa gtgtacgaa 
      atgaacgaa ttaattgtc gctgttaaa aataatgca 
      ctggaaaaa attgccacg attttaggt tcgaaaaca 
      ttggacgtc aacagcaaa atcccagaa tgccaagac 
      ggtacaata ctccatata gcggccgaa aaaggcaat 
      gtttcaatt gtccatatg ctggtcgta gccgggaca 
      caaaccgat gtttacgac aaagatcaa aacacaccg 
      ttaatggcg gccgtaatg gccgccaat aacgaaact 
      gtccgttat ttaataaaa acaggcgcc gacatccat 
      cttaaaggc actgacggc atgacagca cttcatcta 
      gcggcaaaa tgtggtaat ttacacgca tgtcaacta 
      ctcgttgac aactcgcca cgtaaagct gtcaattgt 
      caagatgac ggcggttgg acacccctt gtatgggcg 
      tgtgagcat ggttacggc gacgttgcc acatttctg 
      attaacaaa ggggcggat ccgtctgta cgtgacgtt 
      gaacataat gttgcactt cattgggct gcgtttagt 
      ggaagttgt catattgcc ggtctctta ttggataag 
      ggatgtgac gtcaatgcg gttaacgca catggtgac 
      accccctta catatcggt gcacgtcaa gatatgtac 
      gattgtaac gttgtttta ttggcgcgt ggtgctgac 
      gtcacactt ttaaataaa aataacgaa tcgccattg 
      gattgtgta ccggctggt ggagataca tacagtgtg 
      attgcgtta aatgtgaca ctcggtggc gcctacaat 
      agtcaaaat gtcgttttg tgcaatgat attaccaaa 
      ggacgggaa agtaacccg attcagtgt tacaatagt 
      gttgacaat gccaccaac cctaatgat tttaagtac 
      gtgactaaa aattgtata acgtcagat gacgtgaaa 
      atcgaggcg aaaatcaca gatttacag tgttgccaa 
      tgtgaggaa cgttgtgtc actgatgat tgtcaatgt 
      ggtaaatta tcgttgcgt tgttggtac gatgaggaa 
      ggtaaattg attcccgaa tttaatttt ggcgatatt 
      ccaatgatt ttcgaatgt aacgacaga tgtcagtgt 
      aacgcaatc acgtgtaat aatcgagtc gtccaaaaa 
      gggcccaat caaagattt gaattgttt aaaacactt 
      gataaaggg tggggcata cgtacgctc cgccccata 
      tcacgtggt agcttcata tgtgaatac attggtgag 
      attatcacg gattctgaa gctgataag cgtgaagat 
      gacagtttt ttattcgat ttggagaat cgagtgtgc 
      aagaatgag gacgtagat tcctattgt atcgacgca 
      aaattctac ggtaacttt gcgcgtttt atcaatcat 
      tcctgtaat cccaatttg acatctgtc aaagttttt 
      atcgatcat caggatttg agatttcca aggattgct 
      ttttttgca aatcgggat atttcaaac gaggaggag 
      ttaagtttc gattatgga gaaaagttt tggttggcc 
      aaatataag ttgttttcg tgcctttgt ggcagtttg 
      gagtgcaag tattccgag gatacgatt ggaatgagg 
      acttaa
    mRNA sequence
    • >TC011127 RA
      aaacaattt gttgacatt tccactttt ccgaatcaa 
      ttttccaaa gaatgtaat cagccccat ttaagtgcc 
      cccataatg aacagtggt gaaagtgag tccgaatcg 
      agtaattcc ggcagtgag gacaagtca acaatcatc 
      cacaaaatc cttggcgaa atgaaaagt gaattcaac 
      agcccccga gctgacgac gcccccaat accaacgac 
      gaagaaaaa ccccgaatt attctctcc tttaaaaaa 
      cctgcttcg gatgagtcg cccccagtt ggggggcgg 
      cgctccctc cgttacaaa aacccggag gaaaagccc 
      gaattgaaa cgttctgca aggaggcgc agtaaagac 
      tgctccgag tcggtttta cagagcgct attgcacgt 
      aaagaaaag tcctacaat gagtcaaat aagccccaa 
      agacttaca agacaattg aaaccgact cagaaaatt 
      ttagaaaat ttagcgttg gctaaaatg gaaaagagt 
      cggtttttt gaatgtgag gagagcgaa agacttgta 
      agaaaacac aaacataag cataacaag tgtcggagg 
      ctgaaaagg gccaaattg gggcaaggg gacagcgag 
      aaagagact gattcggat gaagggggc tgtcggagg 
      ccgaggagc gaaaatgtg gacgaaggg gaggagttg 
      gagggggcc aaagacacg tgcttgtgt cggagaagg 
      acgaagttt tttctcgca acgaatgaa gagacggcc 
      tactgcgcc gccgtggac agcatcgat gataaattg 
      attggttgt ggtcgcact gtagccacg aaagacttg 
      atgttgttg cgaccttca acccgtgtt ccatacacg 
      attttttgc gatttgcat aaaagtcgt ctcttgcgt 
      cataattgt tgtccaacg tgtggtatt ttttgtaca 
      cagggacgt ttcgtccaa tgtgagaac aaacatcag 
      tatcataaa gactgtcaa agtgaagtt gattgttgt 
      ccacattgt ggtatttct ggtatttcg tatcaagtt 
      gatatcgta atgcaattg gggaaaaaa ccggttttt 
      ttgcccgta caaaaagcg caatatatt acggcaaga 
      atgacattt tcgacttta aaaacagaa gaatccaaa 
      tgtatgacg ccacctttg atcccaatc ggatcgttt 
      gaatttgac aattcattc gaatatggc gacgaagtg 
      tacgaaatg aacgaatta attgtcgct gttaaaaat 
      aatgcactg gaaaaaatt gccacgatt ttaggttcg 
      aaaacattg gacgtcaac agcaaaatc ccagaatgc 
      caagacggt acaatactc catatagcg gccgaaaaa 
      ggcaatgtt tcaattgtc catatgctg gtcgtagcc 
      gggacacaa accgatgtt tacgacaaa gatcaaaac 
      acaccgtta atggcggcc gtaatggcc gccaataac 
      gaaactgtc cgttattta ataaaaaca ggcgccgac 
      atccatctt aaaggcact gacggcatg acagcactt 
      catctagcg gcaaaatgt ggtaattta cacgcatgt 
      caactactc gttgacaac tcgccacgt aaagctgtc 
      aattgtcaa gatgacggc ggttggaca ccccttgta 
      tgggcgtgt gagcatggt tacggcgac gttgccaca 
      tttctgatt aacaaaggg gcggatccg tctgtacgt 
      gacgttgaa cataatgtt gcacttcat tgggctgcg 
      tttagtgga agttgtcat attgccggt ctcttattg 
      gataaggga tgtgacgtc aatgcggtt aacgcacat 
      ggtgacacc cccttacat atcggtgca cgtcaagat 
      atgtacgat tgtaacgtt gttttattg gcgcgtggt 
      gctgacgtc acactttta aataaaaat aacgaatcg 
      ccattggat tgtgtaccg gctggtgga gatacatac 
      agtgtgatt gcgttaaat gtgacactc ggtggcgcc 
      tacaatagt caaaatgtc gttttgtgc aatgatatt 
      accaaagga cgggaaagt aacccgatt cagtgttac 
      aatagtgtt gacaatgcc accaaccct aatgatttt 
      aagtacgtg actaaaaat tgtataacg tcagatgac 
      gtgaaaatc gaggcgaaa atcacagat ttacagtgt 
      tgccaatgt gaggaacgt tgtgtcact gatgattgt 
      caatgtggt aaattatcg ttgcgttgt tggtacgat 
      gaggaaggt aaattgatt cccgaattt aattttggc 
      gatattcca atgattttc gaatgtaac gacagatgt 
      cagtgtaac gcaatcacg tgtaataat cgagtcgtc 
      caaaaaggg cccaatcaa agatttgaa ttgtttaaa 
      acacttgat aaagggtgg ggcatacgt acgctccgc 
      cccatatca cgtggtagc ttcatatgt gaatacatt 
      ggtgagatt atcacggat tctgaagct gataagcgt 
      gaagatgac agtttttta ttcgatttg gagaatcga 
      gtgtgcaag aatgaggac gtagattcc tattgtatc 
      gacgcaaaa ttctacggt aactttgcg cgttttatc 
      aatcattcc tgtaatccc aatttgaca tctgtcaaa 
      gtttttatc gatcatcag gatttgaga tttccaagg 
      attgctttt tttgcaaat cgggatatt tcaaacgag 
      gaggagtta agtttcgat tatggagaa aagttttgg 
      ttggccaaa tataagttg ttttcgtgc ctttgtggc 
      agtttggag tgcaagtat tccgaggat acgattgga 
      atgaggact taagttttt gaggatagg atttgttat 
      ttcttgcca attaattcc attgtaaac gactgattt 
      cgttattta tttgtgttt tgtaagttt atttatttg 
      gaaggttta ataaaacaa ttgaaagta t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY