| General information | |||
| TC011127 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007425.3: 4254414..4258267 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC011127 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_07793 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC011127 RA
MNSGESESE SSNSGSEDK STIIHKILG EMKSEFNSP RADDAPNTN DEEKPRIIL SFKKPASDE SPPVGGRRS LRYKNPEEK PELKRSARR RSKDCSESV LQSAIARKE KSYNESNKP QRLTRQLKP TQKILENLA LAKMEKSRF FECEESERL VRKHKHKHN KCRRLKRAK LGQGDSEKE TDSDEGGCR RPRSENVDE GEELEGAKD TCLCRRRTK FFLATNEET AYCAAVDSI DDKLIGCGR TVATKDLML LRPSTRVPY TIFCDLHKS RLLRHNCCP TCGIFCTQG RFVQCENKH QYHKDCQSE VDCCPHCGI SGISYQVDI VMQLGKKPV FLPVQKAQY ITARMTFST LKTEESKCM TPPLIPIGS FEFDNSFEY GDEVYEMNE LIVAVKNNA LEKIATILG SKTLDVNSK IPECQDGTI LHIAAEKGN VSIVHMLVV AGTQTDVYD KDQNTPLMA AVMAANNET VRYLIKTGA DIHLKGTDG MTALHLAAK CGNLHACQL LVDNSPRKA VNCQDDGGW TPLVWACEH GYGDVATFL INKGADPSV RDVEHNVAL HWAAFSGSC HIAGLLLDK GCDVNAVNA HGDTPLHIG ARQDMYDCN VVLLARGAD VTLLNKNNE SPLDCVPAG GDTYSVIAL NVTLGGAYN SQNVVLCND ITKGRESNP IQCYNSVDN ATNPNDFKY VTKNCITSD DVKIEAKIT DLQCCQCEE RCVTDDCQC GKLSLRCWY DEEGKLIPE FNFGDIPMI FECNDRCQC NAITCNNRV VQKGPNQRF ELFKTLDKG WGIRTLRPI SRGSFICEY IGEIITDSE ADKREDDSF LFDLENRVC KNEDVDSYC IDAKFYGNF ARFINHSCN PNLTSVKVF IDHQDLRFP RIAFFANRD ISNEEELSF DYGEKFWLA KYKLFSCLC GSLECKYSE DTIGMRT
- >TC011127 RA
atgaacagt ggtgaaagt gagtccgaa tcgagtaat tccggcagt gaggacaag tcaacaatc atccacaaa atccttggc gaaatgaaa agtgaattc aacagcccc cgagctgac gacgccccc aataccaac gacgaagaa aaaccccga attattctc tcctttaaa aaacctgct tcggatgag tcgccccca gttgggggg cggcgctcc ctccgttac aaaaacccg gaggaaaag cccgaattg aaacgttct gcaaggagg cgcagtaaa gactgctcc gagtcggtt ttacagagc gctattgca cgtaaagaa aagtcctac aatgagtca aataagccc caaagactt acaagacaa ttgaaaccg actcagaaa attttagaa aatttagcg ttggctaaa atggaaaag agtcggttt tttgaatgt gaggagagc gaaagactt gtaagaaaa cacaaacat aagcataac aagtgtcgg aggctgaaa agggccaaa ttggggcaa ggggacagc gagaaagag actgattcg gatgaaggg ggctgtcgg aggccgagg agcgaaaat gtggacgaa ggggaggag ttggagggg gccaaagac acgtgcttg tgtcggaga aggacgaag ttttttctc gcaacgaat gaagagacg gcctactgc gccgccgtg gacagcatc gatgataaa ttgattggt tgtggtcgc actgtagcc acgaaagac ttgatgttg ttgcgacct tcaacccgt gttccatac acgattttt tgcgatttg cataaaagt cgtctcttg cgtcataat tgttgtcca acgtgtggt attttttgt acacaggga cgtttcgtc caatgtgag aacaaacat cagtatcat aaagactgt caaagtgaa gttgattgt tgtccacat tgtggtatt tctggtatt tcgtatcaa gttgatatc gtaatgcaa ttggggaaa aaaccggtt tttttgccc gtacaaaaa gcgcaatat attacggca agaatgaca ttttcgact ttaaaaaca gaagaatcc aaatgtatg acgccacct ttgatccca atcggatcg tttgaattt gacaattca ttcgaatat ggcgacgaa gtgtacgaa atgaacgaa ttaattgtc gctgttaaa aataatgca ctggaaaaa attgccacg attttaggt tcgaaaaca ttggacgtc aacagcaaa atcccagaa tgccaagac ggtacaata ctccatata gcggccgaa aaaggcaat gtttcaatt gtccatatg ctggtcgta gccgggaca caaaccgat gtttacgac aaagatcaa aacacaccg ttaatggcg gccgtaatg gccgccaat aacgaaact gtccgttat ttaataaaa acaggcgcc gacatccat cttaaaggc actgacggc atgacagca cttcatcta gcggcaaaa tgtggtaat ttacacgca tgtcaacta ctcgttgac aactcgcca cgtaaagct gtcaattgt caagatgac ggcggttgg acacccctt gtatgggcg tgtgagcat ggttacggc gacgttgcc acatttctg attaacaaa ggggcggat ccgtctgta cgtgacgtt gaacataat gttgcactt cattgggct gcgtttagt ggaagttgt catattgcc ggtctctta ttggataag ggatgtgac gtcaatgcg gttaacgca catggtgac accccctta catatcggt gcacgtcaa gatatgtac gattgtaac gttgtttta ttggcgcgt ggtgctgac gtcacactt ttaaataaa aataacgaa tcgccattg gattgtgta ccggctggt ggagataca tacagtgtg attgcgtta aatgtgaca ctcggtggc gcctacaat agtcaaaat gtcgttttg tgcaatgat attaccaaa ggacgggaa agtaacccg attcagtgt tacaatagt gttgacaat gccaccaac cctaatgat tttaagtac gtgactaaa aattgtata acgtcagat gacgtgaaa atcgaggcg aaaatcaca gatttacag tgttgccaa tgtgaggaa cgttgtgtc actgatgat tgtcaatgt ggtaaatta tcgttgcgt tgttggtac gatgaggaa ggtaaattg attcccgaa tttaatttt ggcgatatt ccaatgatt ttcgaatgt aacgacaga tgtcagtgt aacgcaatc acgtgtaat aatcgagtc gtccaaaaa gggcccaat caaagattt gaattgttt aaaacactt gataaaggg tggggcata cgtacgctc cgccccata tcacgtggt agcttcata tgtgaatac attggtgag attatcacg gattctgaa gctgataag cgtgaagat gacagtttt ttattcgat ttggagaat cgagtgtgc aagaatgag gacgtagat tcctattgt atcgacgca aaattctac ggtaacttt gcgcgtttt atcaatcat tcctgtaat cccaatttg acatctgtc aaagttttt atcgatcat caggatttg agatttcca aggattgct ttttttgca aatcgggat atttcaaac gaggaggag ttaagtttc gattatgga gaaaagttt tggttggcc aaatataag ttgttttcg tgcctttgt ggcagtttg gagtgcaag tattccgag gatacgatt ggaatgagg acttaa
- >TC011127 RA
aaacaattt gttgacatt tccactttt ccgaatcaa ttttccaaa gaatgtaat cagccccat ttaagtgcc cccataatg aacagtggt gaaagtgag tccgaatcg agtaattcc ggcagtgag gacaagtca acaatcatc cacaaaatc cttggcgaa atgaaaagt gaattcaac agcccccga gctgacgac gcccccaat accaacgac gaagaaaaa ccccgaatt attctctcc tttaaaaaa cctgcttcg gatgagtcg cccccagtt ggggggcgg cgctccctc cgttacaaa aacccggag gaaaagccc gaattgaaa cgttctgca aggaggcgc agtaaagac tgctccgag tcggtttta cagagcgct attgcacgt aaagaaaag tcctacaat gagtcaaat aagccccaa agacttaca agacaattg aaaccgact cagaaaatt ttagaaaat ttagcgttg gctaaaatg gaaaagagt cggtttttt gaatgtgag gagagcgaa agacttgta agaaaacac aaacataag cataacaag tgtcggagg ctgaaaagg gccaaattg gggcaaggg gacagcgag aaagagact gattcggat gaagggggc tgtcggagg ccgaggagc gaaaatgtg gacgaaggg gaggagttg gagggggcc aaagacacg tgcttgtgt cggagaagg acgaagttt tttctcgca acgaatgaa gagacggcc tactgcgcc gccgtggac agcatcgat gataaattg attggttgt ggtcgcact gtagccacg aaagacttg atgttgttg cgaccttca acccgtgtt ccatacacg attttttgc gatttgcat aaaagtcgt ctcttgcgt cataattgt tgtccaacg tgtggtatt ttttgtaca cagggacgt ttcgtccaa tgtgagaac aaacatcag tatcataaa gactgtcaa agtgaagtt gattgttgt ccacattgt ggtatttct ggtatttcg tatcaagtt gatatcgta atgcaattg gggaaaaaa ccggttttt ttgcccgta caaaaagcg caatatatt acggcaaga atgacattt tcgacttta aaaacagaa gaatccaaa tgtatgacg ccacctttg atcccaatc ggatcgttt gaatttgac aattcattc gaatatggc gacgaagtg tacgaaatg aacgaatta attgtcgct gttaaaaat aatgcactg gaaaaaatt gccacgatt ttaggttcg aaaacattg gacgtcaac agcaaaatc ccagaatgc caagacggt acaatactc catatagcg gccgaaaaa ggcaatgtt tcaattgtc catatgctg gtcgtagcc gggacacaa accgatgtt tacgacaaa gatcaaaac acaccgtta atggcggcc gtaatggcc gccaataac gaaactgtc cgttattta ataaaaaca ggcgccgac atccatctt aaaggcact gacggcatg acagcactt catctagcg gcaaaatgt ggtaattta cacgcatgt caactactc gttgacaac tcgccacgt aaagctgtc aattgtcaa gatgacggc ggttggaca ccccttgta tgggcgtgt gagcatggt tacggcgac gttgccaca tttctgatt aacaaaggg gcggatccg tctgtacgt gacgttgaa cataatgtt gcacttcat tgggctgcg tttagtgga agttgtcat attgccggt ctcttattg gataaggga tgtgacgtc aatgcggtt aacgcacat ggtgacacc cccttacat atcggtgca cgtcaagat atgtacgat tgtaacgtt gttttattg gcgcgtggt gctgacgtc acactttta aataaaaat aacgaatcg ccattggat tgtgtaccg gctggtgga gatacatac agtgtgatt gcgttaaat gtgacactc ggtggcgcc tacaatagt caaaatgtc gttttgtgc aatgatatt accaaagga cgggaaagt aacccgatt cagtgttac aatagtgtt gacaatgcc accaaccct aatgatttt aagtacgtg actaaaaat tgtataacg tcagatgac gtgaaaatc gaggcgaaa atcacagat ttacagtgt tgccaatgt gaggaacgt tgtgtcact gatgattgt caatgtggt aaattatcg ttgcgttgt tggtacgat gaggaaggt aaattgatt cccgaattt aattttggc gatattcca atgattttc gaatgtaac gacagatgt cagtgtaac gcaatcacg tgtaataat cgagtcgtc caaaaaggg cccaatcaa agatttgaa ttgtttaaa acacttgat aaagggtgg ggcatacgt acgctccgc cccatatca cgtggtagc ttcatatgt gaatacatt ggtgagatt atcacggat tctgaagct gataagcgt gaagatgac agtttttta ttcgatttg gagaatcga gtgtgcaag aatgaggac gtagattcc tattgtatc gacgcaaaa ttctacggt aactttgcg cgttttatc aatcattcc tgtaatccc aatttgaca tctgtcaaa gtttttatc gatcatcag gatttgaga tttccaagg attgctttt tttgcaaat cgggatatt tcaaacgag gaggagtta agtttcgat tatggagaa aagttttgg ttggccaaa tataagttg ttttcgtgc ctttgtggc agtttggag tgcaagtat tccgaggat acgattgga atgaggact taagttttt gaggatagg atttgttat ttcttgcca attaattcc attgtaaac gactgattt cgttattta tttgtgttt tgtaagttt atttatttg gaaggttta ataaaacaa ttgaaagta t
