Status
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Gene information
General information
TC011127 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 4254414..4258267
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC011127
    iBeetle screen iB_07793

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011127 RA
      MNSGESESE SSNSGSEDK STIIHKILG EMKSEFNSP 
RADDAPNTN DEEKPRIIL SFKKPASDE SPPVGGRRS 
LRYKNPEEK PELKRSARR RSKDCSESV LQSAIARKE 
KSYNESNKP QRLTRQLKP TQKILENLA LAKMEKSRF 
FECEESERL VRKHKHKHN KCRRLKRAK LGQGDSEKE 
TDSDEGGCR RPRSENVDE GEELEGAKD TCLCRRRTK 
FFLATNEET AYCAAVDSI DDKLIGCGR TVATKDLML 
LRPSTRVPY TIFCDLHKS RLLRHNCCP TCGIFCTQG 
RFVQCENKH QYHKDCQSE VDCCPHCGI SGISYQVDI 
VMQLGKKPV FLPVQKAQY ITARMTFST LKTEESKCM 
TPPLIPIGS FEFDNSFEY GDEVYEMNE LIVAVKNNA 
LEKIATILG SKTLDVNSK IPECQDGTI LHIAAEKGN 
VSIVHMLVV AGTQTDVYD KDQNTPLMA AVMAANNET 
VRYLIKTGA DIHLKGTDG MTALHLAAK CGNLHACQL 
LVDNSPRKA VNCQDDGGW TPLVWACEH GYGDVATFL 
INKGADPSV RDVEHNVAL HWAAFSGSC HIAGLLLDK 
GCDVNAVNA HGDTPLHIG ARQDMYDCN VVLLARGAD 
VTLLNKNNE SPLDCVPAG GDTYSVIAL NVTLGGAYN 
SQNVVLCND ITKGRESNP IQCYNSVDN ATNPNDFKY 
VTKNCITSD DVKIEAKIT DLQCCQCEE RCVTDDCQC 
GKLSLRCWY DEEGKLIPE FNFGDIPMI FECNDRCQC 
NAITCNNRV VQKGPNQRF ELFKTLDKG WGIRTLRPI 
SRGSFICEY IGEIITDSE ADKREDDSF LFDLENRVC 
KNEDVDSYC IDAKFYGNF ARFINHSCN PNLTSVKVF 
IDHQDLRFP RIAFFANRD ISNEEELSF DYGEKFWLA 
KYKLFSCLC GSLECKYSE DTIGMRT
    CDS sequence
    • >TC011127 RA
      atgaacagt ggtgaaagt gagtccgaa tcgagtaat 
tccggcagt gaggacaag tcaacaatc atccacaaa 
atccttggc gaaatgaaa agtgaattc aacagcccc 
cgagctgac gacgccccc aataccaac gacgaagaa 
aaaccccga attattctc tcctttaaa aaacctgct 
tcggatgag tcgccccca gttgggggg cggcgctcc 
ctccgttac aaaaacccg gaggaaaag cccgaattg 
aaacgttct gcaaggagg cgcagtaaa gactgctcc 
gagtcggtt ttacagagc gctattgca cgtaaagaa 
aagtcctac aatgagtca aataagccc caaagactt 
acaagacaa ttgaaaccg actcagaaa attttagaa 
aatttagcg ttggctaaa atggaaaag agtcggttt 
tttgaatgt gaggagagc gaaagactt gtaagaaaa 
cacaaacat aagcataac aagtgtcgg aggctgaaa 
agggccaaa ttggggcaa ggggacagc gagaaagag 
actgattcg gatgaaggg ggctgtcgg aggccgagg 
agcgaaaat gtggacgaa ggggaggag ttggagggg 
gccaaagac acgtgcttg tgtcggaga aggacgaag 
ttttttctc gcaacgaat gaagagacg gcctactgc 
gccgccgtg gacagcatc gatgataaa ttgattggt 
tgtggtcgc actgtagcc acgaaagac ttgatgttg 
ttgcgacct tcaacccgt gttccatac acgattttt 
tgcgatttg cataaaagt cgtctcttg cgtcataat 
tgttgtcca acgtgtggt attttttgt acacaggga 
cgtttcgtc caatgtgag aacaaacat cagtatcat 
aaagactgt caaagtgaa gttgattgt tgtccacat 
tgtggtatt tctggtatt tcgtatcaa gttgatatc 
gtaatgcaa ttggggaaa aaaccggtt tttttgccc 
gtacaaaaa gcgcaatat attacggca agaatgaca 
ttttcgact ttaaaaaca gaagaatcc aaatgtatg 
acgccacct ttgatccca atcggatcg tttgaattt 
gacaattca ttcgaatat ggcgacgaa gtgtacgaa 
atgaacgaa ttaattgtc gctgttaaa aataatgca 
ctggaaaaa attgccacg attttaggt tcgaaaaca 
ttggacgtc aacagcaaa atcccagaa tgccaagac 
ggtacaata ctccatata gcggccgaa aaaggcaat 
gtttcaatt gtccatatg ctggtcgta gccgggaca 
caaaccgat gtttacgac aaagatcaa aacacaccg 
ttaatggcg gccgtaatg gccgccaat aacgaaact 
gtccgttat ttaataaaa acaggcgcc gacatccat 
cttaaaggc actgacggc atgacagca cttcatcta 
gcggcaaaa tgtggtaat ttacacgca tgtcaacta 
ctcgttgac aactcgcca cgtaaagct gtcaattgt 
caagatgac ggcggttgg acacccctt gtatgggcg 
tgtgagcat ggttacggc gacgttgcc acatttctg 
attaacaaa ggggcggat ccgtctgta cgtgacgtt 
gaacataat gttgcactt cattgggct gcgtttagt 
ggaagttgt catattgcc ggtctctta ttggataag 
ggatgtgac gtcaatgcg gttaacgca catggtgac 
accccctta catatcggt gcacgtcaa gatatgtac 
gattgtaac gttgtttta ttggcgcgt ggtgctgac 
gtcacactt ttaaataaa aataacgaa tcgccattg 
gattgtgta ccggctggt ggagataca tacagtgtg 
attgcgtta aatgtgaca ctcggtggc gcctacaat 
agtcaaaat gtcgttttg tgcaatgat attaccaaa 
ggacgggaa agtaacccg attcagtgt tacaatagt 
gttgacaat gccaccaac cctaatgat tttaagtac 
gtgactaaa aattgtata acgtcagat gacgtgaaa 
atcgaggcg aaaatcaca gatttacag tgttgccaa 
tgtgaggaa cgttgtgtc actgatgat tgtcaatgt 
ggtaaatta tcgttgcgt tgttggtac gatgaggaa 
ggtaaattg attcccgaa tttaatttt ggcgatatt 
ccaatgatt ttcgaatgt aacgacaga tgtcagtgt 
aacgcaatc acgtgtaat aatcgagtc gtccaaaaa 
gggcccaat caaagattt gaattgttt aaaacactt 
gataaaggg tggggcata cgtacgctc cgccccata 
tcacgtggt agcttcata tgtgaatac attggtgag 
attatcacg gattctgaa gctgataag cgtgaagat 
gacagtttt ttattcgat ttggagaat cgagtgtgc 
aagaatgag gacgtagat tcctattgt atcgacgca 
aaattctac ggtaacttt gcgcgtttt atcaatcat 
tcctgtaat cccaatttg acatctgtc aaagttttt 
atcgatcat caggatttg agatttcca aggattgct 
ttttttgca aatcgggat atttcaaac gaggaggag 
ttaagtttc gattatgga gaaaagttt tggttggcc 
aaatataag ttgttttcg tgcctttgt ggcagtttg 
gagtgcaag tattccgag gatacgatt ggaatgagg 
acttaa
    mRNA sequence
    • >TC011127 RA
      aaacaattt gttgacatt tccactttt ccgaatcaa 
ttttccaaa gaatgtaat cagccccat ttaagtgcc 
cccataatg aacagtggt gaaagtgag tccgaatcg 
agtaattcc ggcagtgag gacaagtca acaatcatc 
cacaaaatc cttggcgaa atgaaaagt gaattcaac 
agcccccga gctgacgac gcccccaat accaacgac 
gaagaaaaa ccccgaatt attctctcc tttaaaaaa 
cctgcttcg gatgagtcg cccccagtt ggggggcgg 
cgctccctc cgttacaaa aacccggag gaaaagccc 
gaattgaaa cgttctgca aggaggcgc agtaaagac 
tgctccgag tcggtttta cagagcgct attgcacgt 
aaagaaaag tcctacaat gagtcaaat aagccccaa 
agacttaca agacaattg aaaccgact cagaaaatt 
ttagaaaat ttagcgttg gctaaaatg gaaaagagt 
cggtttttt gaatgtgag gagagcgaa agacttgta 
agaaaacac aaacataag cataacaag tgtcggagg 
ctgaaaagg gccaaattg gggcaaggg gacagcgag 
aaagagact gattcggat gaagggggc tgtcggagg 
ccgaggagc gaaaatgtg gacgaaggg gaggagttg 
gagggggcc aaagacacg tgcttgtgt cggagaagg 
acgaagttt tttctcgca acgaatgaa gagacggcc 
tactgcgcc gccgtggac agcatcgat gataaattg 
attggttgt ggtcgcact gtagccacg aaagacttg 
atgttgttg cgaccttca acccgtgtt ccatacacg 
attttttgc gatttgcat aaaagtcgt ctcttgcgt 
cataattgt tgtccaacg tgtggtatt ttttgtaca 
cagggacgt ttcgtccaa tgtgagaac aaacatcag 
tatcataaa gactgtcaa agtgaagtt gattgttgt 
ccacattgt ggtatttct ggtatttcg tatcaagtt 
gatatcgta atgcaattg gggaaaaaa ccggttttt 
ttgcccgta caaaaagcg caatatatt acggcaaga 
atgacattt tcgacttta aaaacagaa gaatccaaa 
tgtatgacg ccacctttg atcccaatc ggatcgttt 
gaatttgac aattcattc gaatatggc gacgaagtg 
tacgaaatg aacgaatta attgtcgct gttaaaaat 
aatgcactg gaaaaaatt gccacgatt ttaggttcg 
aaaacattg gacgtcaac agcaaaatc ccagaatgc 
caagacggt acaatactc catatagcg gccgaaaaa 
ggcaatgtt tcaattgtc catatgctg gtcgtagcc 
gggacacaa accgatgtt tacgacaaa gatcaaaac 
acaccgtta atggcggcc gtaatggcc gccaataac 
gaaactgtc cgttattta ataaaaaca ggcgccgac 
atccatctt aaaggcact gacggcatg acagcactt 
catctagcg gcaaaatgt ggtaattta cacgcatgt 
caactactc gttgacaac tcgccacgt aaagctgtc 
aattgtcaa gatgacggc ggttggaca ccccttgta 
tgggcgtgt gagcatggt tacggcgac gttgccaca 
tttctgatt aacaaaggg gcggatccg tctgtacgt 
gacgttgaa cataatgtt gcacttcat tgggctgcg 
tttagtgga agttgtcat attgccggt ctcttattg 
gataaggga tgtgacgtc aatgcggtt aacgcacat 
ggtgacacc cccttacat atcggtgca cgtcaagat 
atgtacgat tgtaacgtt gttttattg gcgcgtggt 
gctgacgtc acactttta aataaaaat aacgaatcg 
ccattggat tgtgtaccg gctggtgga gatacatac 
agtgtgatt gcgttaaat gtgacactc ggtggcgcc 
tacaatagt caaaatgtc gttttgtgc aatgatatt 
accaaagga cgggaaagt aacccgatt cagtgttac 
aatagtgtt gacaatgcc accaaccct aatgatttt 
aagtacgtg actaaaaat tgtataacg tcagatgac 
gtgaaaatc gaggcgaaa atcacagat ttacagtgt 
tgccaatgt gaggaacgt tgtgtcact gatgattgt 
caatgtggt aaattatcg ttgcgttgt tggtacgat 
gaggaaggt aaattgatt cccgaattt aattttggc 
gatattcca atgattttc gaatgtaac gacagatgt 
cagtgtaac gcaatcacg tgtaataat cgagtcgtc 
caaaaaggg cccaatcaa agatttgaa ttgtttaaa 
acacttgat aaagggtgg ggcatacgt acgctccgc 
cccatatca cgtggtagc ttcatatgt gaatacatt 
ggtgagatt atcacggat tctgaagct gataagcgt 
gaagatgac agtttttta ttcgatttg gagaatcga 
gtgtgcaag aatgaggac gtagattcc tattgtatc 
gacgcaaaa ttctacggt aactttgcg cgttttatc 
aatcattcc tgtaatccc aatttgaca tctgtcaaa 
gtttttatc gatcatcag gatttgaga tttccaagg 
attgctttt tttgcaaat cgggatatt tcaaacgag 
gaggagtta agtttcgat tatggagaa aagttttgg 
ttggccaaa tataagttg ttttcgtgc ctttgtggc 
agtttggag tgcaagtat tccgaggat acgattgga 
atgaggact taagttttt gaggatagg atttgttat 
ttcttgcca attaattcc attgtaaac gactgattt 
cgttattta tttgtgttt tgtaagttt atttatttg 
gaaggttta ataaaacaa ttgaaagta t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY