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Gene information
General information
TC008558 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450687.1: 4..1726
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC008558
      iBeetle screen iB_07788

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
      abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC008558 RA
        MSYLEGRRI EVTPNHIVD ITCGVPQGS VLGPSLWNI 
        LYDGVLKQD MPLGVKLVA FADDLAVAA IGRNEEDLM 
        RNTNQALAR VASWLEENE LKLAAEKTE AVLLVGRRR 
        PRAVQFQIM GAKIEPKKV IKYLGIYID QACTFSDHL 
        AKTAAKAEG TISALSRIM PNIGGPGSN RRKLLAGVA 
        TSMLLYGCE VWHTALATK KNVVVLEST HRRILARVA 
        CAYRTVSTE AAQVITGVP PARLLVEER VKQRCTGMS 
        KSMARSHTL NLWQENWQQ TTKARWTRR LIPDIEKWV 
        KRKHGEIDY HLTQALTGH GCFGTYLCR IGKILNASC 
        WYCGEEDTP SHTLFSCSY FDPLRMDVM KKILVWPEE 
        SNLIEEMLE SPEKWDVLH TFVKKIMTQ KEKKERELE 
        AGGVRSGQV RSGQVRSGP RVKFGWSSI GRLPRWRIG 
        ESLPDMGGT GEIKYPGRT KTSQDHTVT PDVVWKRGP 
        RGFGAARFR EPL
      CDS sequence
      • >TC008558 RA
        atgagttat ctggagggt aggaggatc gaagtaact 
        ccaaaccac attgtggac attacctgc ggagtcccc 
        caaggctca gttcttggg ccgtcccta tggaatata 
        ttatatgat ggggtatta aagcaggat atgcccctg 
        ggagttaaa ctagtagcc ttcgcagat gacctggcg 
        gtggctgct ataggacgc aacgaggaa gatcttatg 
        aggaacaca aatcaggcc ctcgcacgg gtagcaagc 
        tggttggaa gaaaacgaa ctgaagctg gccgcggaa 
        aaaacggag gctgtgctg ctcgttgga agacgacga 
        cccagggcg gttcagttc caaataatg ggtgccaag 
        atcgagcca aagaaagtg ataaaatac ctcggtatt 
        tatatagac caagcgtgt actttttct gaccatctc 
        gccaagacc gcagcaaaa gcagagggg acaatatcg 
        gcattgagt cgcattatg ccaaacatt ggaggacct 
        gggagtaac aggcgaaaa cttctcgca ggggtggca 
        acttcaatg ctcctttat ggctgcgag gtatggcac 
        acggcgcta gcaacgaaa aagaatgta gtcgtacta 
        gaaagcact cataggagg atcctggcg cgcgtcgct 
        tgcgcttac cgcacggtt tcgacagaa gcggcacaa 
        gtcattact ggagtgccg ccagcccgg ctgctggtc 
        gaagaaagg gttaagcaa agatgtaca ggcatgtca 
        aaatcaatg gcccgctca cacacgcta aatctatgg 
        caggaaaac tggcagcag accaccaaa gctaggtgg 
        accagaaga ttaatccct gatatcgag aaatgggta 
        aagagaaaa catggggaa attgactat catctgacg 
        caggcgcta acggggcat gggtgcttc ggtacctac 
        ctgtgccgc attggcaaa atactaaat gcaagttgc 
        tggtattgt ggcgaggag gacactccg tcacataca 
        ttattttcg tgctcgtac ttcgatcca ctgaggatg 
        gacgtgatg aagaaaatt ctagtgtgg ccagaagaa 
        agtaatctt attgaggag atgttagag tccccagag 
        aaatgggat gttctacac acttttgtg aagaaaatc 
        atgacgcaa aaagaaaaa aaggaaaga gagttagag 
        gccggagga gtcaggtca ggtcaggtc aggtcaggt 
        caggtcagg tcaggtccc cgtgtgaag tttggctgg 
        tcttccatc gggcgcctc cccaggtgg cggataggg 
        gaaagcctc ccagatatg ggtggtacc ggggaaatc 
        aaatacccg gggcggacc aaaaccagt caagatcac 
        accgtaact ccagacgtg gtctggaaa cgggggccg 
        cgcggtttc ggggctgcg aggtttaga gagcctcta 
        taa
      mRNA sequence
      • >TC008558 RA
        ttagactca ttatgagtt atctggagg gtaggagga 
        tcgaagtaa ctccaaacc acattgtgg acattacct 
        gcggagtcc cccaaggct cagttcttg ggccgtccc 
        tatggaata tattatatg atggggtat taaagcagg 
        atatgcccc tgggagtta aactagtag ccttcgcag 
        atgacctgg cggtggctg ctataggac gcaacgagg 
        aagatctta tgaggaaca caaatcagg ccctcgcac 
        gggtagcaa gctggttgg aagaaaacg aactgaagc 
        tggccgcgg aaaaaacgg aggctgtgc tgctcgttg 
        gaagacgac gacccaggg cggttcagt tccaaataa 
        tgggtgcca agatcgagc caaagaaag tgataaaat 
        acctcggta tttatatag accaagcgt gtacttttt 
        ctgaccatc tcgccaaga ccgcagcaa aagcagagg 
        ggacaatat cggcattga gtcgcatta tgccaaaca 
        ttggaggac ctgggagta acaggcgaa aacttctcg 
        caggggtgg caacttcaa tgctccttt atggctgcg 
        aggtatggc acacggcgc tagcaacga aaaagaatg 
        tagtcgtac tagaaagca ctcatagga ggatcctgg 
        cgcgcgtcg cttgcgctt accgcacgg tttcgacag 
        aagcggcac aagtcatta ctggagtgc cgccagccc 
        ggctgctgg tcgaagaaa gggttaagc aaagatgta 
        caggcatgt caaaatcaa tggcccgct cacacacgc 
        taaatctat ggcaggaaa actggcagc agaccacca 
        aagctaggt ggaccagaa gattaatcc ctgatatcg 
        agaaatggg taaagagaa aacatgggg aaattgact 
        atcatctga cgcaggcgc taacggggc atgggtgct 
        tcggtacct acctgtgcc gcattggca aaatactaa 
        atgcaagtt gctggtatt gtggcgagg aggacactc 
        cgtcacata cattatttt cgtgctcgt acttcgatc 
        cactgagga tggacgtga tgaagaaaa ttctagtgt 
        ggccagaag aaagtaatc ttattgagg agatgttag 
        agtccccag agaaatggg atgttctac acacttttg 
        tgaagaaaa tcatgacgc aaaaagaaa aaaaggaaa 
        gagagttag aggccggag gagtcaggt caggtcagg 
        tcaggtcag gtcaggtca ggtcaggtc cccgtgtga 
        agtttggct ggtcttcca tcgggcgcc tccccaggt 
        ggcggatag gggaaagcc tcccagata tgggtggta 
        ccggggaaa tcaaatacc cggggcgga ccaaaacca 
        gtcaagatc acaccgtaa ctccagacg tggtctgga 
        aacgggggc cgcgcggtt tcggggctg cgaggttta 
        gagagcctc tataaaatt ctctctaat caaaataag 
        acaatcatg agcgacgaa tcaaggaag aaacttacg 
        gtgcaagg
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY