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Gene information
General information
TC010568 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451448.1: 111928..122623
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010568
    iBeetle screen iB_07774

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Fertility
    mature egg not present - Number of animals showing the phenotype: 2
    yolk late sometimesamount decreased - Number of animals showing the phenotype: 2

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010568 RA
      MTFLQDTLV VLLSQVTFF VGAWIFFVK QLFKDYEVR 
      HIMVQLIFS ITLTLSCTM FELIIFEIL ELLEASSRY 
      FYWCLMLYL LLFVVIVLN PFYIAYYCI SNIPYVKPD 
      YIKHLTVLV WIIFIVIFW KFGDPFPIL SPKQGVLSM 
      EQLVSRIGV IGVTVMALL SGFGAVNYP YTSMAYFIR 
      EVSLTDVMN IEKRLMQAM DMIVIKKKR IAVAKRQSL 
      EKMNEGMTR RSGLWSMIY PSGNNRNQE NIAQLKLEI 
      AGLEELSRQ LFLEAHENR NMFERIEWS KTWKGIYFN 
      FLGYIFSVY CTWKIFICT INIVFDRVG KKDPVTRGI 
      EIAIHWMGF NFDVNFWSQ QVSFYLVGC IVVTSIRGL 
      LLTLTKFFN RMSSSKSSN IIVLILAQI MGMYFVSSV 
      LLMRMNMPP QYRTIITQV LGDLQFNFY HRWFDVIFL 
      VSALGSIVT LYLAHKQPV HENL
    CDS sequence
    • >TC010568 RA
      atgactttt ttgcaagat acattggta gtgttactt 
      tcccaggta acgtttttc gtgggagca tggatattt 
      ttcgtaaaa caactgttc aaagactac gaagtcagg 
      catataatg gtacaatta attttctca atcacattg 
      actttatca tgtaccatg ttcgaactg attattttt 
      gagattttg gaactattg gaggcaagc tctcgctat 
      ttctactgg tgcctaatg ttgtattta ttattgttt 
      gtggtaatt gttttaaac ccattttat atcgcgtac 
      tattgtatt agtaacatc ccatatgtt aagcctgat 
      tacataaag catttaact gtgttagtg tggataata 
      tttattgta atattttgg aaatttggt gacccattc 
      cctattctg agccccaaa cagggggtc ttatcaatg 
      gaacagcta gtttcacga attggtgtc ataggggtc 
      actgtaatg gccctcctt tcggggttt ggtgccgtt 
      aactaccct tacacatct atggcttac ttcattaga 
      gaagtttct cttactgac gtaatgaac attgagaaa 
      agactgatg caagcgatg gatatgata gttattaaa 
      aagaagcgc attgctgtt gcaaaaaga caatctctt 
      gagaaaatg aatgaaggc atgaccaga aggagcggt 
      ttatggagc atgatatat cccagtggt aacaacaga 
      aatcaagaa aacatagca caactgaag ctggaaata 
      gcagggctt gaagaatta agcaggcaa ctgttttta 
      gaagctcat gaaaatcgc aatatgttt gagaggatt 
      gaatggtcc aagacatgg aaaggaatt tattttaat 
      tttttgggg tacattttt tcagtttac tgtacctgg 
      aaaattttt atatgtaca attaatata gtatttgat 
      agagtaggg aaaaaggat cctgttaca agaggtatt 
      gagattgcc atccactgg atgggattc aatttcgac 
      gtgaatttt tggtcgcaa caagtttcg ttttattta 
      gttggatgt attgttgtc acgtcaatc agaggttta 
      ctactaacg ttgactaaa ttttttaac agaatgtcg 
      tcaagcaag agttccaac ataatcgta cttatttta 
      gcccaaata atgggcatg tacttcgta tcatcagtg 
      cttctaatg cgaatgaat atgccccca caataccga 
      actatcatc actcaagtt ttgggagat ttacaattt 
      aatttctac catcgttgg tttgacgtg atattttta 
      gtaagcgct ttgggcagc attgttacg ctctatttg 
      gcccataaa caaccagtg catgaaaat ttataa
    mRNA sequence
    • >TC010568 RA
      tgtataaca aaaaagtta aatattaat tcaataatc 
      acgggattt ttaaatatt attttatgt taatagaaa 
      ttgagtgca acaacatca caagattaa aaaaattaa 
      ataaaacag cactcaccg tttttgttt ttgcaaggg 
      cacacgatt tgggtttcc ggactaatg ccgttggtt 
      gcaatggcg agctccttc atttcacaa accaaaaac 
      caaaatttc gaaattcac aattcgcgc caaaaactt 
      tggagaaat tgcccggta cacccctgt gctcagtgg 
      ggcaacaaa acaactgtc gggagttta catgatctt 
      gacacacac aaaaaatat ttttcccct ttttaaatg 
      tattgccaa cctaaacag taatttcca cccacccag 
      tgtgacgtt cccatgctg acgtttgat gtctgtcaa 
      aataaaact aaccttaaa attgcacca aatgaaatg 
      aagcttttt tcccgattt cttgcacac agttttata 
      taatcactc aaagttgat caagaaatc aaacagaac 
      atgtaataa ccccacaat tatgacttt tttgcaaga 
      tacattggt agtgttact ttcccaggt aacgttttt 
      cgtgggagc atggatatt tttcgtaaa acaactgtt 
      caaagacta cgaagtcag gcatataat ggtacaatt 
      aattttctc aatcacatt gactttatc atgtaccat 
      gttcgaact gattatttt tgagatttt ggaactatt 
      ggaggcaag ctctcgcta tttctactg gtgcctaat 
      gttgtattt attattgtt tgtggtaat tgttttaaa 
      cccatttta tatcgcgta ctattgtat tagtaacat 
      cccatatgt taagcctga ttacataaa gcatttaac 
      tgtgttagt gtggataat atttattgt aatattttg 
      gaaatttgg tgacccatt ccctattct gagccccaa 
      acagggggt cttatcaat ggaacagct agtttcacg 
      aattggtgt cataggggt cactgtaat ggccctcct 
      ttcggggtt tggtgccgt taactaccc ttacacatc 
      tatggctta cttcattag agaagtttc tcttactga 
      cgtaatgaa cattgagaa aagactgat gcaagcgat 
      ggatatgat agttattaa aaagaagcg cattgctgt 
      tgcaaaaag acaatctct tgagaaaat gaatgaagg 
      catgaccag aaggagcgg tttatggag catgatata 
      tcccagtgg taacaacag aaatcaaga aaacatagc 
      acaactgaa gctggaaat agcagggct tgaagaatt 
      aagcaggca actgttttt agaagctca tgaaaatcg 
      caatatgtt tgagaggat tgaatggtc caagacatg 
      gaaaggaat ttattttaa ttttttggg gtacatttt 
      ttcagttta ctgtacctg gaaaatttt tatatgtac 
      aattaatat agtatttga tagagtagg gaaaaagga 
      tcctgttac aagaggtat tgagattgc catccactg 
      gatgggatt caatttcga cgtgaattt ttggtcgca 
      acaagtttc gttttattt agttggatg tattgttgt 
      cacgtcaat cagaggttt actactaac gttgactaa 
      attttttaa cagaatgtc gtcaagcaa gagttccaa 
      cataatcgt acttatttt agcccaaat aatgggcat 
      gtacttcgt atcatcagt gcttctaat gcgaatgaa 
      tatgccccc acaataccg aactatcat cactcaagt 
      tttgggaga tttacaatt taatttcta ccatcgttg 
      gtttgacgt gatattttt agtaagcgc tttgggcag 
      cattgttac gctctattt ggcccataa acaaccagt 
      gcatgaaaa tttataatt gaaatcttg tgataaata 
      aaggaaata aaaggttcg tttttac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY