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Gene information
General information
TC011144 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 3998676..4004853
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011144
    iBeetle screen iB_07772

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    Adult morphology
    abdominal odoriferous glands content decreased - Number of animals showing the phenotype: 4 (13 dpi)
    Days post injection



    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    mesothorax tracheal opening potentially size decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    abdomen tracheal opening potentially size decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011144 RA
      MDENILGYE TIDEASSLL QNGILVETG KGLVCMSLN 
      DAISSGLNL SVEDLKKVA NELISQQNV GTKDKVCVK 
      SSINLDSDG FSSECSDVS FTSTPSTSS SYITFTSLS 
      NSELTTTSV TKDTKIVTF LPVKSLPPD FKQEPKTVG 
      SEVVPNPPI TTIDVHKPP KRRGGWPKG RKRKPELKN 
      LPPKAPATG YNLYLNDQR KLFKDSKLA FHEITKVIG 
      NKWSNLSLE EKRPYLAKA EEDKRRYRE ELKQYRQSD 
      AYRAYLAKK RRNRLQNNV LSESDMDAT DEIDEEDNE 
      ELYCRTCDQ WFHNLHNKR EHLQGRQHT QSVAGDIKR 
      ELDLSASDS GIFSTSLDE SSLDAMPNI SKHEDKSSS 
      VSDAVVNLV AAVARRERE IKSLQRKLQ ETIMAQSAF 
      CQQLNALRQ RHCKLKNDL SSLKEHERN MEAKVAQLW 
      QVPSWFIIT DFNTDFNDE NK
    CDS sequence
    • >TC011144 RA
      atggacgag aatatcttg gggtatgag accatagat 
      gaggcttcc agtctgttg cagaatgga attcttgtt 
      gagactggc aaaggcctg gtttgtatg tcgttaaat 
      gacgcgatc tcctccggt ctgaattta tcagttgag 
      gatttgaaa aaggtggca aatgagtta atttcgcaa 
      caaaatgtt ggaaccaag gacaaagtt tgtgtcaaa 
      tcgtccata aatctggac agtgatggg ttttcgagt 
      gaatgtagt gacgtgagc ttcacttca acgcccagc 
      acctcctcc agttacatc acttttacc tcactatca 
      aactctgaa cttacaacc acaagtgtt acaaaagac 
      accaaaatc gtcactttc ctcccagtt aagtctcta 
      cctccagac ttcaaacaa gagcctaag acagttggc 
      agtgaagtt gtccccaac cctcctatt accaccatt 
      gacgtccac aaaccccca aagagacgc gggggatgg 
      cccaaaggc cggaaacgg aagccggaa ctgaaaaat 
      ttgccgccg aaagcaccc gccaccgga tacaatctc 
      tatctcaat gaccagcgg aagctgttt aaggacagc 
      aaactggcg tttcatgaa attaccaaa gttatcggg 
      aacaaatgg tcgaatttg agtttggag gagaaacgg 
      ccgtatttg gccaaagca gaggaggac aaaaggcgc 
      tacagggaa gaacttaaa cagtatcga caatcagac 
      gcatatcgc gcgtatttg gccaaaaaa cggcggaat 
      cggctgcag aataacgtg ctgtcagag agcgacatg 
      gacgccacc gatgaaatc gacgaggaa gataacgag 
      gagttgtac tgccgcaca tgcgaccag tggttccac 
      aacttgcac aacaagcgc gagcattta cagggccgc 
      caacacacg caatcagtg gccggcgac atcaagcgc 
      gaattggac ctttcggcg tccgatagc ggcattttt 
      agcacgtct ttggacgaa tctagtctc gacgcaatg 
      cctaacata tcaaaacat gaagacaag tcaagctcg 
      gtatctgat gccgtggtt aatttagtc gcggctgtt 
      gctaggcgg gagagagaa atcaaatcg ttgcaaagg 
      aaactccaa gagacaata atggcacaa agtgcgttc 
      tgccaacag ttaaatgcg ctgagacag aggcactgt 
      aaactgaaa aatgatttg agtagtttg aaggaacac 
      gaaaggaat atggaagcg aaggtggcg cagctgtgg 
      caagtgccc agctggttc atcatcacg gattttaac 
      accgatttt aacgatgaa aacaagtaa
    mRNA sequence
    • >TC011144 RA
      aatggcagc acgtgtccg catgttgtt gtggtttat 
      agggacgtt ttaacgccg atttttcgg tatttaagg 
      gttcaagag tccctaaaa ccaccgatt caccgacga 
      aataaaata accaatcgg tacgaatcg aaaaaaccg 
      taaaaaatc gcccacaag tgtaattta tcgcacttt 
      gactgttgc acatgctcg agttgacaa tcagcaaga 
      aaaagatgt tgattaact gaagacgcc aaatatgga 
      cgagaatat cttggggta tgagaccat agatgaggc 
      ttccagtct gttgcagaa tggaattct tgttgagac 
      tggcaaagg cctggtttg tatgtcgtt aaatgacgc 
      gatctcctc cggtctgaa tttatcagt tgaggattt 
      gaaaaaggt ggcaaatga gttaatttc gcaacaaaa 
      tgttggaac caaggacaa agtttgtgt caaatcgtc 
      cataaatct ggacagtga tgggttttc gagtgaatg 
      tagtgacgt gagcttcac ttcaacgcc cagcacctc 
      ctccagtta catcacttt tacctcact atcaaactc 
      tgaacttac aaccacaag tgttacaaa agacaccaa 
      aatcgtcac tttcctccc agttaagtc tctacctcc 
      agacttcaa acaagagcc taagacagt tggcagtga 
      agttgtccc caaccctcc tattaccac cattgacgt 
      ccacaaacc cccaaagag acgcggggg atggcccaa 
      aggccggaa acggaagcc ggaactgaa aaatttgcc 
      gccgaaagc acccgccac cggatacaa tctctatct 
      caatgacca gcggaagct gtttaagga cagcaaact 
      ggcgtttca tgaaattac caaagttat cgggaacaa 
      atggtcgaa tttgagttt ggaggagaa acggccgta 
      tttggccaa agcagagga ggacaaaag gcgctacag 
      ggaagaact taaacagta tcgacaatc agacgcata 
      tcgcgcgta tttggccaa aaaacggcg gaatcggct 
      gcagaataa cgtgctgtc agagagcga catggacgc 
      caccgatga aatcgacga ggaagataa cgaggagtt 
      gtactgccg cacatgcga ccagtggtt ccacaactt 
      gcacaacaa gcgcgagca tttacaggg ccgccaaca 
      cacgcaatc agtggccgg cgacatcaa gcgcgaatt 
      ggacctttc ggcgtccga tagcggcat ttttagcac 
      gtctttgga cgaatctag tctcgacgc aatgcctaa 
      catatcaaa acatgaaga caagtcaag ctcggtatc 
      tgatgccgt ggttaattt agtcgcggc tgttgctag 
      gcgggagag agaaatcaa atcgttgca aaggaaact 
      ccaagagac aataatggc acaaagtgc gttctgcca 
      acagttaaa tgcgctgag acagaggca ctgtaaact 
      gaaaaatga tttgagtag tttgaagga acacgaaag 
      gaatatgga agcgaaggt ggcgcagct gtggcaagt 
      gcccagctg gttcatcat cacggattt taacaccga 
      ttttaacga tgaaaacaa gtaacaagt taatcagga 
      aaaaaggtt atgggttat ttgtttatt tatttattg 
      ttgactgta cagatggag cgacaggat gttaaaata 
      aaac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY