Status
Please wait ...
Gene information
General information
TC031861 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015452151.1: 1..1599
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC031861
      iBeetle screen iB_07771

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 50
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC031861 RA
        MLLLRLRMT KVAFLASYL YGGVLIRSK FVYKTFLTS 
        SAPPHLPHI DDTLDPNTI DTQVTQLTQ LIKQAQTLF 
        VPIKHIPTN RKPLPPQIL ALIRVKRRI YREFVQTRS 
        PVLKTVFNR LNAQIRRDI NQFRLAHWS NSCSSLDYR 
        DGKKFWDKF NSLTGRKSH PIHHLSVNN TIFNTPQDK 
        ANCFAATLQ NIHQVPNDP HFNQHFFNT VTNNTLRFR 
        QNLPRLDYD ADLDPDPDP DFPPDNPDL TAPTTNDEI 
        KALIIRLRN SKAPGPDQI KPILLKHLP DTAITSITT 
        IYNSCFNSG HFPTPWKEA TTIMIPKPN KDPNNPLSY 
        RPISLLNIL GKIFETILA SRLRHTLEE NNLLPPEQF 
        GFRPHRSTS DPTFELFTD STRSANLGQ CTLAVFLDV 
        ERAFDRVWH DGLIQKFLS HNINLNFIK LIDSFLSNR 
        SCKVKVQNS FSYPVPLLS GF
      CDS sequence
      • >TC031861 RA
        atgctacta ctgcgactg cgaatgact aaagttgcg 
        tttctggct tcgtattta tacggtggg gtcttgatt 
        cggagtaaa ttcgtatat aaaacattt ttaacaagt 
        agtgcacca ccacacctc ccacacatt gacgacaca 
        cttgacccc aacaccatt gacacacaa gtaacacaa 
        ctaacacaa ctcatcaaa caagctcaa acacttttt 
        gtacccatc aaacacatt ccaacaaat cgcaaacca 
        ttgccccct caaatcctt gccctcatc cgagtcaaa 
        cgccgaatt tatcgcgaa ttcgtgcaa accagatct 
        ccagtcctc aaaactgtc tttaaccgt ctcaatgca 
        caaataaga cgtgacatc aaccaattt cgtcttgcc 
        cactggtca aacagttgt agctccctc gattaccga 
        gacggtaaa aagttttgg gacaaattc aattcactc 
        acaggccga aaatctcac cccatccac catttgtca 
        gtcaacaac accattttt aacacccca caagacaaa 
        gcaaattgt tttgctgct accctccaa aacatccac 
        caagtcccc aatgaccca catttcaac caacacttt 
        tttaacaca gtcactaac aacactctc cgtttcagg 
        caaaacctc ccccgactt gactacgac gctgacctc 
        gacccagac cccgaccca gactttcca cccgacaac 
        ccggacttg accgctcca accaccaac gacgagatc 
        aaagccctc attatccgg ctgaggaac tcgaaagcc 
        ccaggacca gaccagatc aaacccatt cttctcaaa 
        catctccct gacacagcc atcacatcc atcaccaca 
        atttacaat tcttgcttc aattccggt cactttcca 
        accccatgg aaagaagcc accaccatc atgattccc 
        aagcccaac aaagacccc aacaaccca ctttcttat 
        cgtcccatc tctctcctc aacattctg ggaaaaatc 
        ttcgaaaca attcttgcc tctcgtctc cgacacact 
        ctcgaagaa aacaaccta ctgccccct gaacaattc 
        ggttttcgc ccccatcgc tccacttcc gaccccact 
        ttcgaactc ttcaccgac tcaacccgg agtgccaat 
        ctcggccag tgcacacta gccgtcttc ttggacgtc 
        gaacgtgca tttgaccgt gtttggcac gatggtctc 
        atccaaaaa tttctttcc cacaacatc aacctcaac 
        ttcatcaaa ttaatcgac tcttttctc tcaaaccgc 
        tcctgcaaa gtcaaagtc caaaattcc ttttcttat 
        cccgtcccc cttctctcc gggttctga
      mRNA sequence
      • >TC031861 RA
        aaagtcaaa ataaactat aagaatatg ctgcgcgta 
        taacttgta tgctactac tgcgactgc gaatgacta 
        aagttgcgt ttctggctt cgtatttat acggtgggg 
        tcttgattc ggagtaaat tcgtatata aaacatttt 
        taacaagta gtgcaccac cacacctcc cacacattg 
        acgacacac ttgacccca acaccattg acacacaag 
        taacacaac taacacaac tcatcaaac aagctcaaa 
        cactttttg tacccatca aacacattc caacaaatc 
        gcaaaccat tgccccctc aaatccttg ccctcatcc 
        gagtcaaac gccgaattt atcgcgaat tcgtgcaaa 
        ccagatctc cagtcctca aaactgtct ttaaccgtc 
        tcaatgcac aaataagac gtgacatca accaatttc 
        gtcttgccc actggtcaa acagttgta gctccctcg 
        attaccgag acggtaaaa agttttggg acaaattca 
        attcactca caggccgaa aatctcacc ccatccacc 
        atttgtcag tcaacaaca ccattttta acaccccac 
        aagacaaag caaattgtt ttgctgcta ccctccaaa 
        acatccacc aagtcccca atgacccac atttcaacc 
        aacactttt ttaacacag tcactaaca acactctcc 
        gtttcaggc aaaacctcc cccgacttg actacgacg 
        ctgacctcg acccagacc ccgacccag actttccac 
        ccgacaacc cggacttga ccgctccaa ccaccaacg 
        acgagatca aagccctca ttatccggc tgaggaact 
        cgaaagccc caggaccag accagatca aacccattc 
        ttctcaaac atctccctg acacagcca tcacatcca 
        tcaccacaa tttacaatt cttgcttca attccggtc 
        actttccaa ccccatgga aagaagcca ccaccatca 
        tgattccca agcccaaca aagacccca acaacccac 
        tttcttatc gtcccatct ctctcctca acattctgg 
        gaaaaatct tcgaaacaa ttcttgcct ctcgtctcc 
        gacacactc tcgaagaaa acaacctac tgccccctg 
        aacaattcg gttttcgcc cccatcgct ccacttccg 
        accccactt tcgaactct tcaccgact caacccgga 
        gtgccaatc tcggccagt gcacactag ccgtcttct 
        tggacgtcg aacgtgcat ttgaccgtg tttggcacg 
        atggtctca tccaaaaat ttctttccc acaacatca 
        acctcaact tcatcaaat taatcgact cttttctct 
        caaaccgct cctgcaaag tcaaagtcc aaaattcct 
        tttcttatc ccgtccccc ttctctccg ggttctgat 
        tttcctgtt tcagatctt caccagacc aaaacccgc 
        ttcttcgcc gacgacacc gccctctgg acatcccgg 
        accaccgcc gcctcagcc tccaggacc c
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY