Status
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Gene information
General information
TC031845
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC012962,TC012960
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015452166.1: 25..6619
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC031845
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
      {{ blurIdResult }}
      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
      {{ blurEvidenceResult }}
      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
      {{ blurReferenceResult }}
      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
      You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
      Email
      We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
      Publish name
      If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_07768

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC031845 RA
        MHAIEKIIQ IAKEANVSK KFNALVLID VRNAFNTAS 
        WPIIVKKLK DIGVQEYLI NLVKSYFNN RTIQVSNDN 
        KKNIGGGVP QGSILGPTL WNIMYNDVM NIETEEDIQ 
        YICYADDLA ISITAKDKE ELMLKTNAT LSAANEWMT 
        QNELKIAPE KNRSHHIRL KNKGRLKPQ DPPKRLGGT 
        GTGKPGVDP SYRRWSVAC LLKTVIAAR IQQPLLSAA 
        GMNINSNPK EGEPTTITE GRESRARNI STTTTEEVS 
        GRRASTGRE AYMGIGNSS QSFDAGDRF LLLDVSGSW 
        SSPKSTQGE LPKTPKRSL KTESPKKEE GRNLGYDTV 
        SVFSSPEEI TDERQPKRK REDTPKSTA QQAGTNDEW 
        KKLCKAVEA THREMERLT SEVSKNPNT KREIKKSIT 
        VLKGLLHRV TVDNLGKLL KDNQTDTYV GDDIKSEKS 
        FQSNETGTQ TEPSMVTIG TQTEDEPSC ACQRDIALI 
        RRWEDQNTE EIRQAIDQQ VTGPELENL FSRSWPEKA 
        YTTTSTKSG SLFSSSTDS DLVIIGDTK DLTNKSVFR 
        KVASRVPHL LALAKENKI KPGKVVHIS CQSEIQVEG 
        DDLEKEHLG QNKRSTFLA TVEESTPSY LEAATNLNK 
        ASMKASSKL LAVAILVDD PKSRENIRK ALEYSLKDL 
        KATIYIPED PTKNRSSRR TANHERVAQ EGIILTANE 
        GKTYADLLK EVKSGLSKS QQEDNLNAN RSRRAHDIL 
        HKTASETNS DIVLLSEPN LKVVDNPRY LHDIRKDTA 
        INILSKHVQ LKCSGSGSG FTWISTSVV TVYSCYISP 
        NSTIGEFIN VLESLTTSM QTQSDPVIV TGDFNAKST 
        EWGMPYSDR RGSLLADWI AQHDLTAAN KHDEYTFQR 
        GLAQSIIDL TLVPTYLVH KLSNWRTLD AESLSDHKY 
        IAFTWDDNA GTTCQTSGY TGWNLKRLD KDKLLSKLR 
        EATCESTPA SLNKILQRA CNAAMPKKR CFRNRRPVY 
        WWNEKIANL RKNALKFRR TITRLRSKR QVSVMLIDG 
        TTSKLKQAQ KMLRDEIQK AKSEKWKEL CLSLEDDLW 
        GNGYQTVMK RTGQACPVK LPREQLNNI VAILFPLHA 
        DVDWIKENC KPIPDFTHD ELRDAARRL KSGKAPGPD 
        GVPGEIIKI MVEERPDTL LEIFNRCAK DSTFPDCWK 
        IARLILLPK PGKLNRTAN AFRPICILD ASGKLYEYL 
        LYKRLDDEI EQRGGLSPF QFGFRKGVS TVHAIDEVI 
        RTAEQEKLP SYRHRKTCL MITFDVQNA FNSASWQII 
        LEELKQKGI SPSLINTIR DYLNNRKII TDYGDTVKV 
        NSGVPQGSV LAALLWNVM YDSVLRIAT SENVKLIGY 
        ADDLAVIIT CKQIDDLEE TANHVVAQI ADWMETKRL 
        KLAPEKTEC ILLRCKRKP PAVKINVLG TEISPKSSI 
        KYLGVWIDQ NCGFKQHIQ QTAIKVEKT ITALSSVMP 
        NIGGPSSSK RRMLSSVAH SAMLYGAPI WHNAMTIES 
        PRSNHFVVV GRLK
      CDS sequence
      • >TC031845 RA
        atgcacgcc attgaaaaa attattcaa atcgcaaaa 
        gaagcaaat gtaagtaag aagtttaat gcgttggta 
        ctaatagat gtccgaaat gctttcaac acggcttcc 
        tggccgatt atcgtaaag aaacttaaa gatataggt 
        gttcaggaa tacctaatc aatctagtc aagtcttac 
        tttaacaac agaactata caagtgtcg aacgataac 
        aagaagaat ataggcggt ggggttccc cagggatca 
        attctcggt ccaacacta tggaacata atgtacaac 
        gacgttatg aacatcgaa acggaggaa gatatccag 
        tacatctgt tatgccgat gatttagct atttcgata 
        acggctaaa gataaagaa gaactgatg ctgaaaacc 
        aatgctact ctgtccgcc gccaatgaa tggatgact 
        cagaacgag ttgaaaatc gctccggaa aaaaacaga 
        agccatcat attcggttg aaaaacaaa ggacggctc 
        aaacctcag gacccacct aagagatta ggtggtacg 
        ggaactggc aaacccggg gtagatccg agctacagg 
        aggtggagc gtagcctgc ctgcttaaa accgtaatt 
        gcggcccga attcaacaa cctttgctg agcgcggca 
        gggatgaat atcaactcc aaccctaaa gaaggtgag 
        cctacaacc ataactgaa ggtcgcgaa agtagagcg 
        cgtaacatc tctacaacg acgacggag gaagttagc 
        ggtcgcaga gccagcact ggacgagaa gcatatatg 
        ggtattggg aatagctca caaagcttt gacgctgga 
        gacaggttt ctactgctg gatgtttct ggatcttgg 
        agcagcccg aaatctaca cagggggaa ctaccgaaa 
        actccaaaa agatcattg aagacagag agcccgaag 
        aaagaagaa ggaagaaac ttgggatac gacaccgtg 
        agtgtattt agctctcca gaagagatt acagatgaa 
        aggcaaccc aagagaaaa cgcgaggac acgcctaaa 
        agcaccgct cagcaagcg ggaacaaac gacgaatgg 
        aagaaatta tgcaaagct gtcgaggca acgcaccga 
        gaaatggag aggcttaca agtgaagtc tcaaagaac 
        ccaaacacc aagagagaa ataaagaag tccataaca 
        gtattaaaa gggctcctt caccgggta actgttgat 
        aaccttggt aagctgctg aaagataac caaacggac 
        acctatgtg ggggacgat ataaaatct gaaaagtca 
        ttccaaagc aatgaaaca ggaacccag actgaacca 
        tctatggtt accatcggc actcaaacg gaagacgaa 
        ccatcttgt gcatgtcaa cgagatatt gccctaatc 
        agaagatgg gaagaccaa aacacggaa gaaatcaga 
        caagctatc gaccaacaa gttactggg cctgaacta 
        gagaactta ttctctcgt tcctggcca gaaaaagca 
        tacacgacc actagtaca aagagtgga agcttattc 
        tcttcgagt accgactca gacttggta ataataggg 
        gatacaaaa gacctgaca aacaaaagc gttttccgg 
        aaagttgca agcagggtc ccacacctc ttagccctc 
        gctaaagag aacaaaatt aagccaggg aaagtcgta 
        catatctcg tgccagagt gaaatccag gtagaggga 
        gacgactta gaaaaggaa catctcggg cagaacaag 
        cggtccaca tttttggca actgtagaa gagtcaact 
        cctagctat cttgaagca gcgactaat ctaaataaa 
        gcttccatg aaggcctcc tccaaacta ctggctgtg 
        gcgatccta gtggacgac cctaaatca agagaaaac 
        atcagaaag gctttagag tactctctt aaagacttg 
        aaggccaca atatacatt cctgaagac ccaaccaag 
        aatagatcc tccagaaga accgccaac catgaacga 
        gtggctcaa gaaggtata attctcaca gctaatgag 
        ggaaaaacc tacgccgac cttttaaaa gaagtcaaa 
        tctgggctt tccaagtct caacaggaa gacaattta 
        aatgcgaac cggagcaga agagcccac gacattctg 
        cacaaaacc gcctctgag accaactcc gacatcgtt 
        ctactaagt gaaccaaac ctgaaggtt gtcgataac 
        ccaagatac ctacacgac attcgaaaa gataccgca 
        attaacatc ctttccaag catgtacaa cttaaatgc 
        tcagggagc gggtcaggg ttcacttgg atctcgaca 
        agtgtagtc accgtgtac agttgctat atctccccc 
        aactctaca atcggggag tttattaac gtgcttgag 
        agtctaacc acctcaatg caaacgcag tcagacccg 
        gtaatcgta accggagac ttcaacgcc aaatccacc 
        gaatggggt atgccatac agtgatagg cgaggatct 
        ctcctggcc gattggata gcacagcac gacctaaca 
        gcggctaat aaacatgat gagtacacc tttcaaaga 
        ggactagcc caatctatt attgaccta acgctagtt 
        ccaacttat ctagttcat aagttgagc aattggaga 
        acccttgat gccgaatcg ttaagcgac cataaatat 
        attgcgttt acgtgggac gataatgcg ggaacgacc 
        tgccaaacc tcaggttat acgggctgg aatctaaaa 
        cgtttagac aaagataag ctactgtcc aaactgaga 
        gaagcaacc tgcgaatca acaccagcg agtctaaat 
        aagatccta cagagagcc tgcaacgcc gctatgcca 
        aaaaaaaga tgtttccga aatcgaaga cccgtctac 
        tggtggaac gaaaaaata gccaacttg cggaaaaat 
        gctttgaag ttccgtcgc accataact cgactccga 
        tctaaaaga caagtctca gtgatgtta atcgatgga 
        actacttcc aaactcaaa caagcacag aagatgcta 
        cgagacgaa atacagaaa gcaaaatct gagaaatgg 
        aaggagttg tgtctatcg ctcgaagac gacctttgg 
        ggaaacggt tatcagaca gtaatgaaa agaacaggc 
        caggcttgc cctgtcaag ctgccgaga gagcaactg 
        aacaacatt gtagccata ctgtttcca ttacacgcc 
        gatgttgac tggatcaaa gaaaactgc aaaccgatc 
        cctgatttt acccacgat gagctgcgg gatgccgcc 
        agaagactc aaatccggc aaagcacca ggtccagat 
        ggggtccct ggagaaatc atcaagatc atggtagaa 
        gaacgcccg gatacgctg cttgaaatc tttaacagg 
        tgcgctaaa gactccaca ttcccggac tgctggaag 
        atcgcacgt ctgattctc ctgccaaaa ccgggcaaa 
        ctaaacaga acagctaat gcattccga ccgatctgc 
        atcctagat gcgtcagga aagctatat gagtaccta 
        ctgtacaag agattagac gacgaaatt gagcaacgc 
        ggagggctc tcaccgttt cagtttggt ttcagaaag 
        ggcgtctca accgttcat gcgattgat gaggttata 
        cggacagcg gaacaggaa aagctacct tcctatagg 
        catagaaag acgtgcctt atgatcact tttgatgtc 
        caaaacgcc tttaattca gcgtcttgg cagataatt 
        ttagaagag ctgaaacag aagggaata tcaccgagc 
        ctaataaat actattaga gattacctc aacaacagg 
        aaaatcatt acagactac ggagacacg gtcaaagtg 
        aacagcgga gttccccaa ggatctgtc cttgccgcc 
        ctactttgg aacgtaatg tatgacagt gtccttcgc 
        atagcaacg tcggaaaat gtcaaactt ataggatac 
        gcagatgac ctggctgtt attatcacc tgtaagcag 
        atagacgac ttggaagaa acagcaaat catgtagta 
        gcgcagata gcagactgg atggagacc aagcgctta 
        aaactagcc cccgagaaa actgaatgc atactcctt 
        agatgcaaa cgcaaacct ccggcagtt aaaattaac 
        gtgttagga acagagatc agcccaaaa agttctata 
        aaatatcta ggggtatgg atagatcag aactgcggt 
        tttaaacaa cacatacaa caaaccgca attaaagtt 
        gaaaagaca attacagcc ttatcatca gtaatgccc 
        aacataggc ggaccaagt tcctcaaag cgaaggatg 
        ctgtcatcc gtcgcccac tcggccatg ttatacggc 
        gcacctata tggcataac gccatgact atagagtcc 
        ccgaggagc aaccacttt gtcgtagtg gggaggctt 
        aagtag
      mRNA sequence
      • >TC031845 RA
        cccgaagtt gttaaaata gtaataaaa aacaatata 
        aattacttt agagactta tacaataac ttgctcacg 
        ggaaaggca agacgacga tgcacgcca ttgaaaaaa 
        ttattcaaa tcgcaaaag aagcaaatg taagtaaga 
        agtttaatg cgttggtac taatagatg tccgaaatg 
        ctttcaaca cggcttcct ggccgatta tcgtaaaga 
        aacttaaag atataggtg ttcaggaat acctaatca 
        atctagtca agtcttact ttaacaaca gaactatac 
        aagtgtcga acgataaca agaagaata taggcggtg 
        gggttcccc agggatcaa ttctcggtc caacactat 
        ggaacataa tgtacaacg acgttatga acatcgaaa 
        cggaggaag atatccagt acatctgtt atgccgatg 
        atttagcta tttcgataa cggctaaag ataaagaag 
        aactgatgc tgaaaacca atgctactc tgtccgccg 
        ccaatgaat ggatgactc agaacgagt tgaaaatcg 
        ctccggaaa aaaacagaa gccatcata ttcggttga 
        aaaacaaag gacggctca aacctcagg acccaccta 
        agagattag gtggtacgg gaactggca aacccgggg 
        tagatccga gctacagga ggtggagcg tagcctgcc 
        tgcttaaaa ccgtaattg cggcccgaa ttcaacaac 
        ctttgctga gcgcggcag ggatgaata tcaactcca 
        accctaaag aaggtgagc ctacaacca taactgaag 
        gtcgcgaaa gtagagcgc gtaacatct ctacaacga 
        cgacggagg aagttagcg gtcgcagag ccagcactg 
        gacgagaag catatatgg gtattggga atagctcac 
        aaagctttg acgctggag acaggtttc tactgctgg 
        atgtttctg gatcttgga gcagcccga aatctacac 
        agggggaac taccgaaaa ctccaaaaa gatcattga 
        agacagaga gcccgaaga aagaagaag gaagaaact 
        tgggatacg acaccgtga gtgtattta gctctccag 
        aagagatta cagatgaaa ggcaaccca agagaaaac 
        gcgaggaca cgcctaaaa gcaccgctc agcaagcgg 
        gaacaaacg acgaatgga agaaattat gcaaagctg 
        tcgaggcaa cgcaccgag aaatggaga ggcttacaa 
        gtgaagtct caaagaacc caaacacca agagagaaa 
        taaagaagt ccataacag tattaaaag ggctccttc 
        accgggtaa ctgttgata accttggta agctgctga 
        aagataacc aaacggaca cctatgtgg gggacgata 
        taaaatctg aaaagtcat tccaaagca atgaaacag 
        gaacccaga ctgaaccat ctatggtta ccatcggca 
        ctcaaacgg aagacgaac catcttgtg catgtcaac 
        gagatattg ccctaatca gaagatggg aagaccaaa 
        acacggaag aaatcagac aagctatcg accaacaag 
        ttactgggc ctgaactag agaacttat tctctcgtt 
        cctggccag aaaaagcat acacgacca ctagtacaa 
        agagtggaa gcttattct cttcgagta ccgactcag 
        acttggtaa taatagggg atacaaaag acctgacaa 
        acaaaagcg ttttccgga aagttgcaa gcagggtcc 
        cacacctct tagccctcg ctaaagaga acaaaatta 
        agccaggga aagtcgtac atatctcgt gccagagtg 
        aaatccagg tagagggag acgacttag aaaaggaac 
        atctcgggc agaacaagc ggtccacat ttttggcaa 
        ctgtagaag agtcaactc ctagctatc ttgaagcag 
        cgactaatc taaataaag cttccatga aggcctcct 
        ccaaactac tggctgtgg cgatcctag tggacgacc 
        ctaaatcaa gagaaaaca tcagaaagg ctttagagt 
        actctctta aagacttga aggccacaa tatacattc 
        ctgaagacc caaccaaga atagatcct ccagaagaa 
        ccgccaacc atgaacgag tggctcaag aaggtataa 
        ttctcacag ctaatgagg gaaaaacct acgccgacc 
        ttttaaaag aagtcaaat ctgggcttt ccaagtctc 
        aacaggaag acaatttaa atgcgaacc ggagcagaa 
        gagcccacg acattctgc acaaaaccg cctctgaga 
        ccaactccg acatcgttc tactaagtg aaccaaacc 
        tgaaggttg tcgataacc caagatacc tacacgaca 
        ttcgaaaag ataccgcaa ttaacatcc tttccaagc 
        atgtacaac ttaaatgct cagggagcg ggtcagggt 
        tcacttgga tctcgacaa gtgtagtca ccgtgtaca 
        gttgctata tctccccca actctacaa tcggggagt 
        ttattaacg tgcttgaga gtctaacca cctcaatgc 
        aaacgcagt cagacccgg taatcgtaa ccggagact 
        tcaacgcca aatccaccg aatggggta tgccataca 
        gtgataggc gaggatctc tcctggccg attggatag 
        cacagcacg acctaacag cggctaata aacatgatg 
        agtacacct ttcaaagag gactagccc aatctatta 
        ttgacctaa cgctagttc caacttatc tagttcata 
        agttgagca attggagaa cccttgatg ccgaatcgt 
        taagcgacc ataaatata ttgcgttta cgtgggacg 
        ataatgcgg gaacgacct gccaaacct caggttata 
        cgggctgga atctaaaac gtttagaca aagataagc 
        tactgtcca aactgagag aagcaacct gcgaatcaa 
        caccagcga gtctaaata agatcctac agagagcct 
        gcaacgccg ctatgccaa aaaaaagat gtttccgaa 
        atcgaagac ccgtctact ggtggaacg aaaaaatag 
        ccaacttgc ggaaaaatg ctttgaagt tccgtcgca 
        ccataactc gactccgat ctaaaagac aagtctcag 
        tgatgttaa tcgatggaa ctacttcca aactcaaac 
        aagcacaga agatgctac gagacgaaa tacagaaag 
        caaaatctg agaaatgga aggagttgt gtctatcgc 
        tcgaagacg acctttggg gaaacggtt atcagacag 
        taatgaaaa gaacaggcc aggcttgcc ctgtcaagc 
        tgccgagag agcaactga acaacattg tagccatac 
        tgtttccat tacacgccg atgttgact ggatcaaag 
        aaaactgca aaccgatcc ctgatttta cccacgatg 
        agctgcggg atgccgcca gaagactca aatccggca 
        aagcaccag gtccagatg gggtccctg gagaaatca 
        tcaagatca tggtagaag aacgcccgg atacgctgc 
        ttgaaatct ttaacaggt gcgctaaag actccacat 
        tcccggact gctggaaga tcgcacgtc tgattctcc 
        tgccaaaac cgggcaaac taaacagaa cagctaatg 
        cattccgac cgatctgca tcctagatg cgtcaggaa 
        agctatatg agtacctac tgtacaaga gattagacg 
        acgaaattg agcaacgcg gagggctct caccgtttc 
        agtttggtt tcagaaagg gcgtctcaa ccgttcatg 
        cgattgatg aggttatac ggacagcgg aacaggaaa 
        agctacctt cctataggc atagaaaga cgtgcctta 
        tgatcactt ttgatgtcc aaaacgcct ttaattcag 
        cgtcttggc agataattt tagaagagc tgaaacaga 
        agggaatat caccgagcc taataaata ctattagag 
        attacctca acaacagga aaatcatta cagactacg 
        gagacacgg tcaaagtga acagcggag ttccccaag 
        gatctgtcc ttgccgccc tactttgga acgtaatgt 
        atgacagtg tccttcgca tagcaacgt cggaaaatg 
        tcaaactta taggatacg cagatgacc tggctgtta 
        ttatcacct gtaagcaga tagacgact tggaagaaa 
        cagcaaatc atgtagtag cgcagatag cagactgga 
        tggagacca agcgcttaa aactagccc ccgagaaaa 
        ctgaatgca tactcctta gatgcaaac gcaaacctc 
        cggcagtta aaattaacg tgttaggaa cagagatca 
        gcccaaaaa gttctataa aatatctag gggtatgga 
        tagatcaga actgcggtt ttaaacaac acatacaac 
        aaaccgcaa ttaaagttg aaaagacaa ttacagcct 
        tatcatcag taatgccca acataggcg gaccaagtt 
        cctcaaagc gaaggatgc tgtcatccg tcgcccact 
        cggccatgt tatacggcg cacctatat ggcataacg 
        ccatgacta tagagtccc cgaggagca accactttg 
        tcgtagtgg ggaggctta agtagctcc aatgattgt 
        tgaggtt
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