| General information | |||
| TC031845
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NW_015452166.1: 25..6619 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC031845 | ||
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_07768 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC031845 RA
MHAIEKIIQ IAKEANVSK KFNALVLID VRNAFNTAS WPIIVKKLK DIGVQEYLI NLVKSYFNN RTIQVSNDN KKNIGGGVP QGSILGPTL WNIMYNDVM NIETEEDIQ YICYADDLA ISITAKDKE ELMLKTNAT LSAANEWMT QNELKIAPE KNRSHHIRL KNKGRLKPQ DPPKRLGGT GTGKPGVDP SYRRWSVAC LLKTVIAAR IQQPLLSAA GMNINSNPK EGEPTTITE GRESRARNI STTTTEEVS GRRASTGRE AYMGIGNSS QSFDAGDRF LLLDVSGSW SSPKSTQGE LPKTPKRSL KTESPKKEE GRNLGYDTV SVFSSPEEI TDERQPKRK REDTPKSTA QQAGTNDEW KKLCKAVEA THREMERLT SEVSKNPNT KREIKKSIT VLKGLLHRV TVDNLGKLL KDNQTDTYV GDDIKSEKS FQSNETGTQ TEPSMVTIG TQTEDEPSC ACQRDIALI RRWEDQNTE EIRQAIDQQ VTGPELENL FSRSWPEKA YTTTSTKSG SLFSSSTDS DLVIIGDTK DLTNKSVFR KVASRVPHL LALAKENKI KPGKVVHIS CQSEIQVEG DDLEKEHLG QNKRSTFLA TVEESTPSY LEAATNLNK ASMKASSKL LAVAILVDD PKSRENIRK ALEYSLKDL KATIYIPED PTKNRSSRR TANHERVAQ EGIILTANE GKTYADLLK EVKSGLSKS QQEDNLNAN RSRRAHDIL HKTASETNS DIVLLSEPN LKVVDNPRY LHDIRKDTA INILSKHVQ LKCSGSGSG FTWISTSVV TVYSCYISP NSTIGEFIN VLESLTTSM QTQSDPVIV TGDFNAKST EWGMPYSDR RGSLLADWI AQHDLTAAN KHDEYTFQR GLAQSIIDL TLVPTYLVH KLSNWRTLD AESLSDHKY IAFTWDDNA GTTCQTSGY TGWNLKRLD KDKLLSKLR EATCESTPA SLNKILQRA CNAAMPKKR CFRNRRPVY WWNEKIANL RKNALKFRR TITRLRSKR QVSVMLIDG TTSKLKQAQ KMLRDEIQK AKSEKWKEL CLSLEDDLW GNGYQTVMK RTGQACPVK LPREQLNNI VAILFPLHA DVDWIKENC KPIPDFTHD ELRDAARRL KSGKAPGPD GVPGEIIKI MVEERPDTL LEIFNRCAK DSTFPDCWK IARLILLPK PGKLNRTAN AFRPICILD ASGKLYEYL LYKRLDDEI EQRGGLSPF QFGFRKGVS TVHAIDEVI RTAEQEKLP SYRHRKTCL MITFDVQNA FNSASWQII LEELKQKGI SPSLINTIR DYLNNRKII TDYGDTVKV NSGVPQGSV LAALLWNVM YDSVLRIAT SENVKLIGY ADDLAVIIT CKQIDDLEE TANHVVAQI ADWMETKRL KLAPEKTEC ILLRCKRKP PAVKINVLG TEISPKSSI KYLGVWIDQ NCGFKQHIQ QTAIKVEKT ITALSSVMP NIGGPSSSK RRMLSSVAH SAMLYGAPI WHNAMTIES PRSNHFVVV GRLK
- >TC031845 RA
atgcacgcc attgaaaaa attattcaa atcgcaaaa gaagcaaat gtaagtaag aagtttaat gcgttggta ctaatagat gtccgaaat gctttcaac acggcttcc tggccgatt atcgtaaag aaacttaaa gatataggt gttcaggaa tacctaatc aatctagtc aagtcttac tttaacaac agaactata caagtgtcg aacgataac aagaagaat ataggcggt ggggttccc cagggatca attctcggt ccaacacta tggaacata atgtacaac gacgttatg aacatcgaa acggaggaa gatatccag tacatctgt tatgccgat gatttagct atttcgata acggctaaa gataaagaa gaactgatg ctgaaaacc aatgctact ctgtccgcc gccaatgaa tggatgact cagaacgag ttgaaaatc gctccggaa aaaaacaga agccatcat attcggttg aaaaacaaa ggacggctc aaacctcag gacccacct aagagatta ggtggtacg ggaactggc aaacccggg gtagatccg agctacagg aggtggagc gtagcctgc ctgcttaaa accgtaatt gcggcccga attcaacaa cctttgctg agcgcggca gggatgaat atcaactcc aaccctaaa gaaggtgag cctacaacc ataactgaa ggtcgcgaa agtagagcg cgtaacatc tctacaacg acgacggag gaagttagc ggtcgcaga gccagcact ggacgagaa gcatatatg ggtattggg aatagctca caaagcttt gacgctgga gacaggttt ctactgctg gatgtttct ggatcttgg agcagcccg aaatctaca cagggggaa ctaccgaaa actccaaaa agatcattg aagacagag agcccgaag aaagaagaa ggaagaaac ttgggatac gacaccgtg agtgtattt agctctcca gaagagatt acagatgaa aggcaaccc aagagaaaa cgcgaggac acgcctaaa agcaccgct cagcaagcg ggaacaaac gacgaatgg aagaaatta tgcaaagct gtcgaggca acgcaccga gaaatggag aggcttaca agtgaagtc tcaaagaac ccaaacacc aagagagaa ataaagaag tccataaca gtattaaaa gggctcctt caccgggta actgttgat aaccttggt aagctgctg aaagataac caaacggac acctatgtg ggggacgat ataaaatct gaaaagtca ttccaaagc aatgaaaca ggaacccag actgaacca tctatggtt accatcggc actcaaacg gaagacgaa ccatcttgt gcatgtcaa cgagatatt gccctaatc agaagatgg gaagaccaa aacacggaa gaaatcaga caagctatc gaccaacaa gttactggg cctgaacta gagaactta ttctctcgt tcctggcca gaaaaagca tacacgacc actagtaca aagagtgga agcttattc tcttcgagt accgactca gacttggta ataataggg gatacaaaa gacctgaca aacaaaagc gttttccgg aaagttgca agcagggtc ccacacctc ttagccctc gctaaagag aacaaaatt aagccaggg aaagtcgta catatctcg tgccagagt gaaatccag gtagaggga gacgactta gaaaaggaa catctcggg cagaacaag cggtccaca tttttggca actgtagaa gagtcaact cctagctat cttgaagca gcgactaat ctaaataaa gcttccatg aaggcctcc tccaaacta ctggctgtg gcgatccta gtggacgac cctaaatca agagaaaac atcagaaag gctttagag tactctctt aaagacttg aaggccaca atatacatt cctgaagac ccaaccaag aatagatcc tccagaaga accgccaac catgaacga gtggctcaa gaaggtata attctcaca gctaatgag ggaaaaacc tacgccgac cttttaaaa gaagtcaaa tctgggctt tccaagtct caacaggaa gacaattta aatgcgaac cggagcaga agagcccac gacattctg cacaaaacc gcctctgag accaactcc gacatcgtt ctactaagt gaaccaaac ctgaaggtt gtcgataac ccaagatac ctacacgac attcgaaaa gataccgca attaacatc ctttccaag catgtacaa cttaaatgc tcagggagc gggtcaggg ttcacttgg atctcgaca agtgtagtc accgtgtac agttgctat atctccccc aactctaca atcggggag tttattaac gtgcttgag agtctaacc acctcaatg caaacgcag tcagacccg gtaatcgta accggagac ttcaacgcc aaatccacc gaatggggt atgccatac agtgatagg cgaggatct ctcctggcc gattggata gcacagcac gacctaaca gcggctaat aaacatgat gagtacacc tttcaaaga ggactagcc caatctatt attgaccta acgctagtt ccaacttat ctagttcat aagttgagc aattggaga acccttgat gccgaatcg ttaagcgac cataaatat attgcgttt acgtgggac gataatgcg ggaacgacc tgccaaacc tcaggttat acgggctgg aatctaaaa cgtttagac aaagataag ctactgtcc aaactgaga gaagcaacc tgcgaatca acaccagcg agtctaaat aagatccta cagagagcc tgcaacgcc gctatgcca aaaaaaaga tgtttccga aatcgaaga cccgtctac tggtggaac gaaaaaata gccaacttg cggaaaaat gctttgaag ttccgtcgc accataact cgactccga tctaaaaga caagtctca gtgatgtta atcgatgga actacttcc aaactcaaa caagcacag aagatgcta cgagacgaa atacagaaa gcaaaatct gagaaatgg aaggagttg tgtctatcg ctcgaagac gacctttgg ggaaacggt tatcagaca gtaatgaaa agaacaggc caggcttgc cctgtcaag ctgccgaga gagcaactg aacaacatt gtagccata ctgtttcca ttacacgcc gatgttgac tggatcaaa gaaaactgc aaaccgatc cctgatttt acccacgat gagctgcgg gatgccgcc agaagactc aaatccggc aaagcacca ggtccagat ggggtccct ggagaaatc atcaagatc atggtagaa gaacgcccg gatacgctg cttgaaatc tttaacagg tgcgctaaa gactccaca ttcccggac tgctggaag atcgcacgt ctgattctc ctgccaaaa ccgggcaaa ctaaacaga acagctaat gcattccga ccgatctgc atcctagat gcgtcagga aagctatat gagtaccta ctgtacaag agattagac gacgaaatt gagcaacgc ggagggctc tcaccgttt cagtttggt ttcagaaag ggcgtctca accgttcat gcgattgat gaggttata cggacagcg gaacaggaa aagctacct tcctatagg catagaaag acgtgcctt atgatcact tttgatgtc caaaacgcc tttaattca gcgtcttgg cagataatt ttagaagag ctgaaacag aagggaata tcaccgagc ctaataaat actattaga gattacctc aacaacagg aaaatcatt acagactac ggagacacg gtcaaagtg aacagcgga gttccccaa ggatctgtc cttgccgcc ctactttgg aacgtaatg tatgacagt gtccttcgc atagcaacg tcggaaaat gtcaaactt ataggatac gcagatgac ctggctgtt attatcacc tgtaagcag atagacgac ttggaagaa acagcaaat catgtagta gcgcagata gcagactgg atggagacc aagcgctta aaactagcc cccgagaaa actgaatgc atactcctt agatgcaaa cgcaaacct ccggcagtt aaaattaac gtgttagga acagagatc agcccaaaa agttctata aaatatcta ggggtatgg atagatcag aactgcggt tttaaacaa cacatacaa caaaccgca attaaagtt gaaaagaca attacagcc ttatcatca gtaatgccc aacataggc ggaccaagt tcctcaaag cgaaggatg ctgtcatcc gtcgcccac tcggccatg ttatacggc gcacctata tggcataac gccatgact atagagtcc ccgaggagc aaccacttt gtcgtagtg gggaggctt aagtag
- >TC031845 RA
cccgaagtt gttaaaata gtaataaaa aacaatata aattacttt agagactta tacaataac ttgctcacg ggaaaggca agacgacga tgcacgcca ttgaaaaaa ttattcaaa tcgcaaaag aagcaaatg taagtaaga agtttaatg cgttggtac taatagatg tccgaaatg ctttcaaca cggcttcct ggccgatta tcgtaaaga aacttaaag atataggtg ttcaggaat acctaatca atctagtca agtcttact ttaacaaca gaactatac aagtgtcga acgataaca agaagaata taggcggtg gggttcccc agggatcaa ttctcggtc caacactat ggaacataa tgtacaacg acgttatga acatcgaaa cggaggaag atatccagt acatctgtt atgccgatg atttagcta tttcgataa cggctaaag ataaagaag aactgatgc tgaaaacca atgctactc tgtccgccg ccaatgaat ggatgactc agaacgagt tgaaaatcg ctccggaaa aaaacagaa gccatcata ttcggttga aaaacaaag gacggctca aacctcagg acccaccta agagattag gtggtacgg gaactggca aacccgggg tagatccga gctacagga ggtggagcg tagcctgcc tgcttaaaa ccgtaattg cggcccgaa ttcaacaac ctttgctga gcgcggcag ggatgaata tcaactcca accctaaag aaggtgagc ctacaacca taactgaag gtcgcgaaa gtagagcgc gtaacatct ctacaacga cgacggagg aagttagcg gtcgcagag ccagcactg gacgagaag catatatgg gtattggga atagctcac aaagctttg acgctggag acaggtttc tactgctgg atgtttctg gatcttgga gcagcccga aatctacac agggggaac taccgaaaa ctccaaaaa gatcattga agacagaga gcccgaaga aagaagaag gaagaaact tgggatacg acaccgtga gtgtattta gctctccag aagagatta cagatgaaa ggcaaccca agagaaaac gcgaggaca cgcctaaaa gcaccgctc agcaagcgg gaacaaacg acgaatgga agaaattat gcaaagctg tcgaggcaa cgcaccgag aaatggaga ggcttacaa gtgaagtct caaagaacc caaacacca agagagaaa taaagaagt ccataacag tattaaaag ggctccttc accgggtaa ctgttgata accttggta agctgctga aagataacc aaacggaca cctatgtgg gggacgata taaaatctg aaaagtcat tccaaagca atgaaacag gaacccaga ctgaaccat ctatggtta ccatcggca ctcaaacgg aagacgaac catcttgtg catgtcaac gagatattg ccctaatca gaagatggg aagaccaaa acacggaag aaatcagac aagctatcg accaacaag ttactgggc ctgaactag agaacttat tctctcgtt cctggccag aaaaagcat acacgacca ctagtacaa agagtggaa gcttattct cttcgagta ccgactcag acttggtaa taatagggg atacaaaag acctgacaa acaaaagcg ttttccgga aagttgcaa gcagggtcc cacacctct tagccctcg ctaaagaga acaaaatta agccaggga aagtcgtac atatctcgt gccagagtg aaatccagg tagagggag acgacttag aaaaggaac atctcgggc agaacaagc ggtccacat ttttggcaa ctgtagaag agtcaactc ctagctatc ttgaagcag cgactaatc taaataaag cttccatga aggcctcct ccaaactac tggctgtgg cgatcctag tggacgacc ctaaatcaa gagaaaaca tcagaaagg ctttagagt actctctta aagacttga aggccacaa tatacattc ctgaagacc caaccaaga atagatcct ccagaagaa ccgccaacc atgaacgag tggctcaag aaggtataa ttctcacag ctaatgagg gaaaaacct acgccgacc ttttaaaag aagtcaaat ctgggcttt ccaagtctc aacaggaag acaatttaa atgcgaacc ggagcagaa gagcccacg acattctgc acaaaaccg cctctgaga ccaactccg acatcgttc tactaagtg aaccaaacc tgaaggttg tcgataacc caagatacc tacacgaca ttcgaaaag ataccgcaa ttaacatcc tttccaagc atgtacaac ttaaatgct cagggagcg ggtcagggt tcacttgga tctcgacaa gtgtagtca ccgtgtaca gttgctata tctccccca actctacaa tcggggagt ttattaacg tgcttgaga gtctaacca cctcaatgc aaacgcagt cagacccgg taatcgtaa ccggagact tcaacgcca aatccaccg aatggggta tgccataca gtgataggc gaggatctc tcctggccg attggatag cacagcacg acctaacag cggctaata aacatgatg agtacacct ttcaaagag gactagccc aatctatta ttgacctaa cgctagttc caacttatc tagttcata agttgagca attggagaa cccttgatg ccgaatcgt taagcgacc ataaatata ttgcgttta cgtgggacg ataatgcgg gaacgacct gccaaacct caggttata cgggctgga atctaaaac gtttagaca aagataagc tactgtcca aactgagag aagcaacct gcgaatcaa caccagcga gtctaaata agatcctac agagagcct gcaacgccg ctatgccaa aaaaaagat gtttccgaa atcgaagac ccgtctact ggtggaacg aaaaaatag ccaacttgc ggaaaaatg ctttgaagt tccgtcgca ccataactc gactccgat ctaaaagac aagtctcag tgatgttaa tcgatggaa ctacttcca aactcaaac aagcacaga agatgctac gagacgaaa tacagaaag caaaatctg agaaatgga aggagttgt gtctatcgc tcgaagacg acctttggg gaaacggtt atcagacag taatgaaaa gaacaggcc aggcttgcc ctgtcaagc tgccgagag agcaactga acaacattg tagccatac tgtttccat tacacgccg atgttgact ggatcaaag aaaactgca aaccgatcc ctgatttta cccacgatg agctgcggg atgccgcca gaagactca aatccggca aagcaccag gtccagatg gggtccctg gagaaatca tcaagatca tggtagaag aacgcccgg atacgctgc ttgaaatct ttaacaggt gcgctaaag actccacat tcccggact gctggaaga tcgcacgtc tgattctcc tgccaaaac cgggcaaac taaacagaa cagctaatg cattccgac cgatctgca tcctagatg cgtcaggaa agctatatg agtacctac tgtacaaga gattagacg acgaaattg agcaacgcg gagggctct caccgtttc agtttggtt tcagaaagg gcgtctcaa ccgttcatg cgattgatg aggttatac ggacagcgg aacaggaaa agctacctt cctataggc atagaaaga cgtgcctta tgatcactt ttgatgtcc aaaacgcct ttaattcag cgtcttggc agataattt tagaagagc tgaaacaga agggaatat caccgagcc taataaata ctattagag attacctca acaacagga aaatcatta cagactacg gagacacgg tcaaagtga acagcggag ttccccaag gatctgtcc ttgccgccc tactttgga acgtaatgt atgacagtg tccttcgca tagcaacgt cggaaaatg tcaaactta taggatacg cagatgacc tggctgtta ttatcacct gtaagcaga tagacgact tggaagaaa cagcaaatc atgtagtag cgcagatag cagactgga tggagacca agcgcttaa aactagccc ccgagaaaa ctgaatgca tactcctta gatgcaaac gcaaacctc cggcagtta aaattaacg tgttaggaa cagagatca gcccaaaaa gttctataa aatatctag gggtatgga tagatcaga actgcggtt ttaaacaac acatacaac aaaccgcaa ttaaagttg aaaagacaa ttacagcct tatcatcag taatgccca acataggcg gaccaagtt cctcaaagc gaaggatgc tgtcatccg tcgcccact cggccatgt tatacggcg cacctatat ggcataacg ccatgacta tagagtccc cgaggagca accactttg tcgtagtgg ggaggctta agtagctcc aatgattgt tgaggtt
