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Gene information
General information
TC005075 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 7208335..7275666
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005075
    iBeetle screen iB_07764

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Adult morphology
    abdominal odoriferous glands content fragmented - Number of animals showing the phenotype: 4 (13 dpi)
    Days post injection



    abdominal odoriferous glands partially colour lighter - Number of animals showing the phenotype: 4 (13 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005075 RA
      MLNTVSMKS SVKRKHLEN WYASRPTKT PTISMLKIQ 
      LVSRHWGRF RLVEVLHVA GNIPWGALE HGVMSTAKR 
      LVLSWRGRK QSHDSSVGR LVGHGGGSA LGPGDNHEL 
      DEIAVVSSN ADPCHVKYG SGTTLSTIN GPRLVPGII 
      TSGGGCMVG ERLSSSGTC LNVSPPVQG GAPATAPHK 
      ASTESVHRV LKSVSRQSL LDLVGCKLG MSKSRQPQS 
      YHQQRYFSS SHDSISTSY INRAANVSS AASELDLSR 
      KARRKDLRG RLKLPTSVT LASSGEAPL LGAWRTAPG 
      STAGTNPPS TNHLNSTSS CNGTESRYG FRRGESYSS 
      RLRTGSANW SFVFDPAGR LCYYWSMVV SLAFLYNFW 
      VIIYRFAFR EIDANTILV WFCLDYFAD FLYLVDIMF 
      HFRTGYLED GVLQTDSTK LRHHYMNST TFYIDCLCL 
      LPLDFLYLS VGFNSILRS FRLVKIYRF WAFMDRTER 
      HTNYPNLFR STSLIHYLL VIFHWNGCL FHIILKNDG 
      FGSKNWVYS DSESTDYVK AYLQSYYWC TLALTTIGD 
      LPRPRNKGE YLFVIFQLL FGLLLFATV LGHVANIVT 
      SVSAARKEF QAKLDGVKT YMRMRRVPN HLQVKVIKW 
      FDYLWLTQK CSDEERAVS CLPDKLKAE IAINVHLDT 
      LKRVEIFQN TEAGFLCEL VLRLRPVLF SPGDYICRK 
      GEVGKEMYI VNRGRLQVV ADNGRTVLA TLKAGSYFG 
      EISILNMGT AGKHLGKYQ QAGPTNRGA ILYSA
    CDS sequence
    • >TC005075 RA
      atgttgaac acggtttcg atgaaatcg tcggtaaaa 
      cggaaacat ttggagaac tggtacgca tcacgtccc 
      acgaagaca ccgactatc tccatgtta aaaattcag 
      ctcgtttcg agacattgg gggcggttt cgtttggtg 
      gaggtcctc cacgtggcg ggcaacata ccctggggc 
      gcgctagaa catggcgtg atgtccact gccaaacga 
      ctggtgctg agttggcgg ggccgaaag cagtcacat 
      gatagctcg gtcgggcgg ctagtggga catggcgga 
      ggcagcgcc ctgggcccc ggggacaac cacgagctt 
      gatgagatc gctgtcgta tccagtaat gccgatcct 
      tgtcacgtc aagtatggc agtggaact acactatcg 
      accatcaat gggcccaga ctcgttccg gggataatc 
      acaagtgga ggaggatgc atggtaggg gagcgttta 
      agcagcagt ggaacgtgt ttgaatgtc agcccgcct 
      gtgcaaggg ggggctccc gccaccgcc ccccacaaa 
      gccagcacg gagagcgtc caccgggtc cttaaatct 
      gtctccagg cagagcctg ctagacctg gtcggctgt 
      aaactagga atgtcaaag agccgtcag cctcaaagc 
      taccatcaa cagcgttac ttttcatcg tctcacgat 
      agcatctca acgtcgtac atcaataga gcagcgaat 
      gttagttcg gccgcgagt gaattggat ctgtcaaga 
      aaagctcga agaaaagac ttgagaggt cgattgaaa 
      ttgcctacg agtgtaacc ctagccagt tccggggaa 
      gcgccgctt cttggtgct tggaggacg gctccgggt 
      tccaccgct ggaactaat ccaccctcc accaatcat 
      ctcaattca acaagcagt tgcaacgga actgaaagc 
      cgctacggc ttcaggaga ggtgaatcg tacagttca 
      aggctgcga acagggtcg gcaaattgg tctttcgtt 
      tttgatcct gcgggaaga ctgtgctat tactggtcc 
      atggtcgtc tccttagcc tttctttac aatttttgg 
      gtcattatt taccgcttt gcctttcga gaaattgat 
      gcgaacact atcctagtg tggttctgc ctggactac 
      ttcgcagac ttcctctac ctggtggac atcatgttc 
      catttccgg accggttac ctggaggac ggcgtgttg 
      caaacggat tcgacgaaa ctgcgacac cactacatg 
      aattccacc acgttctac atagactgt ttgtgcctg 
      ttgcctctc gacttcctg tacttatcc gtgggtttc 
      aactcgatc ttgcgttcg ttccgtttg gtgaaaatc 
      taccgcttc tgggcgttc atggatcgc accgaacgc 
      cacacgaac tacccgaac ttgttcaga tccaccagc 
      ctcatccac tacctccta gttatattc cattggaac 
      ggctgtttg ttccatatc atattaaaa aacgacggc 
      ttcggctct aaaaactgg gtgtacagt gactcggaa 
      agtacggat tacgtgaaa gcgtatttg cagagttat 
      tattggtgc acgctagcg ctgacgacc attggggac 
      ttgcccagg cctaggaac aagggggag tatctcttc 
      gtcatcttc cagctgttg ttcgggctc ctgctcttc 
      gccactgta ttgggtcac gtcgctaac atcgtaacc 
      tccgtttcc gccgctcgc aaggagttt caggcgaaa 
      ctcgacgga gtcaaaacg tatatgaga atgcgcagg 
      gtaccaaac catttgcag gtgaaagtg attaaatgg 
      tttgactat ctatggctc acccaaaag tgttcagat 
      gaagaaaga gccgtatcg tgtcttcca gataagcta 
      aaggctgaa atagcaatc aacgttcat ctagatact 
      ctgaaaagg gtggagatt tttcagaac accgaagcc 
      ggattcctg tgcgagttg gtgctgcgg ctgcggccg 
      gtcctgttc tcccccgga gattacatt tgcagaaaa 
      ggcgaggtc ggcaaggaa atgtatata gtgaaccga 
      ggccgcctt caagtggtc gccgataac gggaggaca 
      gtcctggcc actctgaaa gccgggagt tattttggg 
      gagatttcc atcctcaat atgggcacg gcaggtaaa 
      caccttggt aaatatcag caggcaggc ccaacgaat 
      cggggtgca attctctac tctgcatga
    mRNA sequence
    • >TC005075 RA
      gcagtggaa acacggctg ctgccaggg ttgcgttca 
      gccggcgag atctgcaag atagatctt ttgcttgtg 
      agtgccagt agtcgggtt gaaatgatt gatttgtga 
      caaacgtgt gaccaaact aactgattt actgacagc 
      tgtgggtta tagtgtccc gttaaatat ttgcgctga 
      tatgacacg gacctgtga ttgcaattt atcttatta 
      atatgttga acacggttt cgatgaaat cgtcggtaa 
      aacggaaac atttggaga actggtacg catcacgtc 
      ccacgaaga caccgacta tctccatgt taaaaattc 
      agctcgttt cgagacatt gggggcggt ttcgtttgg 
      tggaggtcc tccacgtgg cgggcaaca taccctggg 
      gcgcgctag aacatggcg tgatgtcca ctgccaaac 
      gactggtgc tgagttggc ggggccgaa agcagtcac 
      atgatagct cggtcgggc ggctagtgg gacatggcg 
      gaggcagcg ccctgggcc ccggggaca accacgagc 
      ttgatgaga tcgctgtcg tatccagta atgccgatc 
      cttgtcacg tcaagtatg gcagtggaa ctacactat 
      cgaccatca atgggccca gactcgttc cggggataa 
      tcacaagtg gaggaggat gcatggtag gggagcgtt 
      taagcagca gtggaacgt gtttgaatg tcagcccgc 
      ctgtgcaag ggggggctc ccgccaccg ccccccaca 
      aagccagca cggagagcg tccaccggg tccttaaat 
      ctgtctcca ggcagagcc tgctagacc tggtcggct 
      gtaaactag gaatgtcaa agagccgtc agcctcaaa 
      gctaccatc aacagcgtt acttttcat cgtctcacg 
      atagcatct caacgtcgt acatcaata gagcagcga 
      atgttagtt cggccgcga gtgaattgg atctgtcaa 
      gaaaagctc gaagaaaag acttgagag gtcgattga 
      aattgccta cgagtgtaa ccctagcca gttccgggg 
      aagcgccgc ttcttggtg cttggagga cggctccgg 
      gttccaccg ctggaacta atccaccct ccaccaatc 
      atctcaatt caacaagca gttgcaacg gaactgaaa 
      gccgctacg gcttcagga gaggtgaat cgtacagtt 
      caaggctgc gaacagggt cggcaaatt ggtctttcg 
      tttttgatc ctgcgggaa gactgtgct attactggt 
      ccatggtcg tctccttag cctttcttt acaattttt 
      gggtcatta tttaccgct ttgcctttc gagaaattg 
      atgcgaaca ctatcctag tgtggttct gcctggact 
      acttcgcag acttcctct acctggtgg acatcatgt 
      tccatttcc ggaccggtt acctggagg acggcgtgt 
      tgcaaacgg attcgacga aactgcgac accactaca 
      tgaattcca ccacgttct acatagact gtttgtgcc 
      tgttgcctc tcgacttcc tgtacttat ccgtgggtt 
      tcaactcga tcttgcgtt cgttccgtt tggtgaaaa 
      tctaccgct tctgggcgt tcatggatc gcaccgaac 
      gccacacga actacccga acttgttca gatccacca 
      gcctcatcc actacctcc tagttatat tccattgga 
      acggctgtt tgttccata tcatattaa aaaacgacg 
      gcttcggct ctaaaaact gggtgtaca gtgactcgg 
      aaagtacgg attacgtga aagcgtatt tgcagagtt 
      attattggt gcacgctag cgctgacga ccattgggg 
      acttgccca ggcctagga acaaggggg agtatctct 
      tcgtcatct tccagctgt tgttcgggc tcctgctct 
      tcgccactg tattgggtc acgtcgcta acatcgtaa 
      cctccgttt ccgccgctc gcaaggagt ttcaggcga 
      aactcgacg gagtcaaaa cgtatatga gaatgcgca 
      gggtaccaa accatttgc aggtgaaag tgattaaat 
      ggtttgact atctatggc tcacccaaa agtgttcag 
      atgaagaaa gagccgtat cgtgtcttc cagataagc 
      taaaggctg aaatagcaa tcaacgttc atctagata 
      ctctgaaaa gggtggaga tttttcaga acaccgaag 
      ccggattcc tgtgcgagt tggtgctgc ggctgcggc 
      cggtcctgt tctcccccg gagattaca tttgcagaa 
      aaggcgagg tcggcaagg aaatgtata tagtgaacc 
      gaggccgcc ttcaagtgg tcgccgata acgggagga 
      cagtcctgg ccactctga aagccggga gttattttg 
      gggagattt ccatcctca atatgggca cggcaggta 
      aacaccttg gtaaatatc agcaggcag gcccaacga 
      atcggggtg caattctct actctgcat gagatcgaa 
      ttttgatat cctgcccgg cttgtattt gtcaaccct 
      tgcaaacta tctttaaat aaatcattc ggggaaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY