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Gene information
General information
TC030320 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 3856717..3858410
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030320
    iBeetle screen iB_07759

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Adult morphology
    abdominal odoriferous glands content fragmented - Number of animals showing the phenotype: 1 (13 dpi)
    Days post injection



    abdominal odoriferous glands size decreased - Number of animals showing the phenotype: 3 (13 dpi)
    Days post injection



    abdominal odoriferous glands content decreased - Number of animals showing the phenotype: 3 (13 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030320 RA
      MDFTIRDFD LRNSFSLER KLLLVLGFY PIRDKEKHR 
      ILHQLSAFL NLLLYYGQL LTIIIQMVI DRNDLSKLT 
      DSTLYFLTL FTFLCKLFN FQYYGKDLI EVEKSLTDP 
      IFYGYSFHK LQIIKAKVR SCTLVCLAF RISCTCSCF 
      IYSVVPFID RSGQKTLSI PGWFPYDTA KHFYITFFL 
      QSLSLFISA HCNSATDTL PCKLISLAT AQFELLKDN 
      LRTIDYENS FEETKHALV KCITHHRKI VNYTKRVET 
      IFSKGIFLQ LFASVLVIC TTGFQLVIV PFGSLKFAI 
      HGIYLCAMT AQIAIYCYY GHDVMITSD EIGTSLYMS 
      NWYASHIKI RKIMVIFLE KTKKPTIVL AGNFITLSL 
      VTLTQILRS AYSYFAVLR RLYADD
    CDS sequence
    • >TC030320 RA
      atggatttt acaatacga gactttgat cttcgtaac 
      tccttctct ctggaacgc aaattacta ctagtcctc 
      ggtttttac ccaataaga gacaaagag aaacacaga 
      attttgcac caattaagt gcctttctc aatctttta 
      ctttactat gggcagctt ttaacaata atcattcag 
      atggtcatc gacaggaac gatttatcc aaactcacc 
      gactccaca ctatatttc ctaacactt ttcaccttt 
      ctgtgcaaa ttattcaat tttcagtac tatggcaag 
      gatttaatc gaagttgaa aaatcccta acagaccca 
      atcttctat ggatattct ttccacaaa ttgcaaata 
      attaaggca aaggttcgc tcctgcact ttggtttgt 
      ctggctttt agaattagc tgcacctgt agttgtttc 
      atctacagc gtggtgcct tttattgac aggagtggg 
      caaaaaact ctgtcaatt cccggctgg tttccttat 
      gatacagcc aaacatttc tatatcacg ttttttctc 
      caaagttta agtttgttt attagtgct cattgtaac 
      tcagccact gatacctta ccctgcaaa cttatatca 
      ctagcgaca gcacagttc gagcttttg aaggataat 
      ttaagaaca attgactat gagaatagt tttgaggaa 
      actaaacac gctcttgtc aagtgtata acgcaccac 
      aggaaaatt gtcaattac accaaacga gttgagacc 
      attttctcc aaaggaata ttcctccaa ttgttcgcc 
      agcgtgtta gttatctgt actacaggt ttccaatta 
      gtgatcgta ccatttggt agtttgaag tttgccatt 
      catgggatt tatttatgt gcaatgact gctcagatt 
      gccatttat tgttactac ggacatgat gtcatgata 
      actagtgat gaaattgga acatcgttg tatatgtcg 
      aattggtac gctagtcat attaaaatt cgcaaaatt 
      atggtgatt tttttggag aaaaccaaa aaaccgacg 
      attgttctg gcggggaat tttatcacc ttgtcgttg 
      gtcacactg acacagatt ttgcgatcg gcctattca 
      tatttcgcg gttttgcga cgtctctat gccgatgat 
      taa
    mRNA sequence
    • >TC030320 RA
      gtagtctca acacaaaca ctaaaatgg attttacaa 
      tacgagact ttgatcttc gtaactcct tctctctgg 
      aacgcaaat tactactag tcctcggtt tttacccaa 
      taagagaca aagagaaac acagaattt tgcaccaat 
      taagtgcct ttctcaatc ttttacttt actatgggc 
      agcttttaa caataatca ttcagatgg tcatcgaca 
      ggaacgatt tatccaaac tcaccgact ccacactat 
      atttcctaa cacttttca cctttctgt gcaaattat 
      tcaattttc agtactatg gcaaggatt taatcgaag 
      ttgaaaaat ccctaacag acccaatct tctatggat 
      attctttcc acaaattgc aaataatta aggcaaagg 
      ttcgctcct gcactttgg tttgtctgg cttttagaa 
      ttagctgca cctgtagtt gtttcatct acagcgtgg 
      tgcctttta ttgacagga gtgggcaaa aaactctgt 
      caattcccg gctggtttc cttatgata cagccaaac 
      atttctata tcacgtttt ttctccaaa gtttaagtt 
      tgtttatta gtgctcatt gtaactcag ccactgata 
      ccttaccct gcaaactta tatcactag cgacagcac 
      agttcgagc ttttgaagg ataatttaa gaacaattg 
      actatgaga atagttttg aggaaacta aacacgctc 
      ttgtcaagt gtataacgc accacagga aaattgtca 
      attacacca aacgagttg agaccattt tctccaaag 
      gaatattcc tccaattgt tcgccagcg tgttagtta 
      tctgtacta caggtttcc aattagtga tcgtaccat 
      ttggtagtt tgaagtttg ccattcatg ggatttatt 
      tatgtgcaa tgactgctc agattgcca tttattgtt 
      actacggac atgatgtca tgataacta gtgatgaaa 
      ttggaacat cgttgtata tgtcgaatt ggtacgcta 
      gtcatatta aaattcgca aaattatgg tgatttttt 
      tggagaaaa ccaaaaaac cgacgattg ttctggcgg 
      ggaatttta tcaccttgt cgttggtca cactgacac 
      agattttgc gatcggcct attcatatt tcgcggttt 
      tgcgacgtc tctatgccg atgattaaa tgttcttga 
      atttggtta atcaataaa gcactgcac ttatc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY