General information | |||
TC001889 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007416.3: 569255..578689 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC001889 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
Gene | {{ goGene }} |
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GO ID | * {{ blurIdResult }} |
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Evidence | {{ blurEvidenceResult }} |
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Reference | * {{ blurReferenceResult }} |
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Quotation | ||
Name | ||
Publish name | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_07748 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
vitellogenic egg chamber late amount increased - Number of animals showing the phenotype: 4
mature egg not present - Number of animals showing the phenotype: 4
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_00309 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC001889 RA
MADRFILSL LVTAFSLSC TLGKEASSP PYIVKQPPT DEVLFQVES GGETDKPFY IECEAIGEP APKYRWVKN GKPFHWQTY DDRISQQQG RGTLSISKP TDVDLGQYQ CFAENEHGV ATSNSVFMR KAELNSFKT TQPISLSGD EGEPFKLTC EPPDGWPKP IVHWIYLYT NGGFKTINS SRMTIDPEG NLWFSNLTR DDVTDNFKY ACAATSPFK HEYKYGNSV LLNVVSTGV SAASNKREP VLQYVSRKN TVAYLGKQQ KLYCIFGGT PLPQTVWYK DGKPIQSSD RITQDNYGK SLVIRVVNF EDEGKYTCE VSNGVGEAK SYSIDLKVF AIPYFIAEP ERQNAAEGE TVEFRCEAG GVPKPEIKW IHNGKPISE APPNPRRRV ENNRIVIER LVESDTGNY GCNATNSLG YVYKDVYVN VLSLAPEIL EAPTDTQAV DGQNVTMIC KVLGAPKPS IKWSLNERE LTGGRYIVQ PNGDLIIRN VQFDDRGNY TCFAENKFG NASAKAALL VKSHTYITD GPEDYEVAA HTSATFRCN AVSDDSLDL EIIWLKNDQ PIDLEEEPR FVRSSDYSL TITQTIELD SGTYTCVAR TALDEASAK AQLIVQDVP NPPSMEGVE CHAKDATIR WKSMGDNRA PISHYIIEY NTTFTPETW TAAYNNVPA TDMSYNVPM SPWTNYTFR VIAVNKIGK SLPSTHSDV CTTEPDVPY KNPDDVKGE GDRPDNLII SWTPMPKIE HNADKFQYR VYWRRDIPG ERYESRDIT DWRVKQFVV DNQPTFQQY RIKVVAMNE KGEANVAPK EVIGYSGEN IPIEAPTNF TLLQIQGPK TALLSWNPV DKKSVQGHL KGYKIKTWS DISSPMHNE IQVQGSVAK ALVDNLDPY TMNYAQVFV YNGKYNGPP SETLSFKTP EGVPEAMSY LEAYPLGAF RPNDATKTA SSAFLLTWK KPEKPNGML TGYDIYYRR LDDSTTNSK IARNPQIRG GDVLSAKLS GLEPDTNYV IYIAARTSA GPSKEFYIE RRTSPSGAS TPPGVPDFD WETVKADDD GKVTVRIQW RPDKTGNPG SHFIVKYKR EAEPNYEEV EVLNDDFVE IPGIAPRES YDFKVTSVD GDYYVDSKV KTINIYSDG PLIKANENM ASAGWFIGM MLAIAILLL ILIVVCIIK RNRGGKYAV HEREQANGR HDYPDEGGF HEYSQPLDN KSHGRASMS SEPKVGPES DTDSMAEYG DGDTEGMNE DGSFIGQYG RKRGQGETT SQGFATLV
- >TC001889 RA
atggcagat cgattcatt ttaagtttg ttagtgact gcgtttagt ttatcatgc acgctagga aaggaagct tcatccccc ccgtacatc gtgaaacag cccccaacc gacgaagtc ctgttccaa gtcgaaagc ggcggcgaa accgacaag cccttctac atcgaatgc gaagcgata ggcgaacct gcaccaaaa taccgttgg gtcaaaaac ggtaaaccc ttccactgg caaacctac gacgaccgc atatcgcaa cagcaaggc cgtggtaca ttatcaatt tccaaaccg accgatgtc gacctcggc caataccaa tgtttcgcc gaaaacgag cacggagtc gccacctcg aactccgta ttcatgcga aaagccgaa ttgaactcg ttcaaaacg acgcaaccg atttcacta tcgggggat gagggcgaa cccttcaaa ctgacgtgc gaaccaccc gatggttgg ccgaaacct atagtgcac tggatttat ctctacacg aatggagga ttcaaaacg attaatagc tcgcgaatg acgatcgat cctgaaggg aatttgtgg ttttcgaat ttgacgagg gatgatgtc accgataat ttcaagtat gcgtgtgcg gcaacctca cccttcaaa cacgaatac aagtatgga aacagcgtc ttgctcaat gttgtctcc accggagtc tccgccgcc tctaataag cgggaaccg gtgttacag tacgtctca aggaaaaat actgtagcc tatttgggg aaacaacaa aaattgtat tgcattttc ggcgggact ccgctgccg cagaccgtg tggtacaaa gacggcaag ccgatccag tcttcggac cgcataact caggataat tacggcaaa tcgctggtt atccgagtg gtgaacttc gaagacgag ggtaaatac acctgcgaa gtgagtaac ggagtcggc gaagcgaaa tcgtattcg atcgatttg aaagttttc gcgattccc tactttatc gctgaaccg gagcgtcaa aacgccgcc gagggcgaa accgtcgag tttcggtgc gaggccggc ggtgtcccc aaacccgaa atcaagtgg attcataat ggaaaaccg ataagtgag gcaccaccc aatccgaga cgacgagtc gagaacaat cgaatcgtt atcgaaaga cttgtcgaa agcgatacc ggaaattac ggatgtaac gctactaat tctttgggt tacgtgtac aaggacgtc tacgtcaat gttctctct ctagcacct gagattctc gaagctccg actgatacc caagcagtc gatggccaa aatgtcact atgatttgt aaggtctta ggggcgcct aaaccctcg attaagtgg agtttgaac gagcgggaa ttgacaggt ggacgctac attgtccag ccaaacggc gatctaatc atcagaaac gtccagttt gacgacagg ggcaactac acctgtttc gccgaaaat aagttcggc aacgccagc gccaaagcg gcactgctc gtcaaatcc cacacatat atcaccgac ggtcctgag gactacgaa gtggcggcg cacacttcg gccaccttt aggtgcaac gcagtcagc gacgattcg ctagacttg gaaataatt tggctgaaa aacgaccag cctatcgac ctagaggag gaaccaaga ttcgtgcgc tcgagtgat tattcgttg accattact caaacaatt gagttagat tcagggact tacacttgc gtggctcga accgcgtta gacgaagcg tctgccaaa gctcaactt attgttcaa gatgtacca aatccgccc tcgatggag ggagtcgag tgtcacgct aaggacgcc accattcgg tggaagtca atgggcgac aaccgggcg ccaatttcg cactatatc atcgaatat aacaccacg ttcactcct gaaacgtgg actgcggct tacaataac gtgccagcg actgatatg agctacaat gttcctatg agcccatgg actaattac actttccga gtaattgcc gttaacaaa atcggaaaa tcgttacct tccacccat tcagatgtt tgcacaact gagccagat gtgccctac aagaaccct gacgatgtc aagggtgaa ggtgacaga cccgataat ctcattatt tcgtggacg ccaatgcca aaaatcgaa cataacgcc gacaaattc caataccgc gtctactgg aggcgtgac ataccaggg gagcgttac gaaagccgc gacattacc gactggagg gttaaacaa ttcgttgtc gataatcaa cccacgttt caacaatat cgcatcaaa gtcgttgcg atgaacgag aagggcgaa gccaacgtc gcaccgaag gaagttata ggctattca ggcgaaaac atccccatc gaagccccc acgaacttc acccttctc caaatccaa ggccccaag acggcgctc cttagctgg aaccccgtt gacaagaag agcgtccaa ggccacttg aaaggctac aaaatcaaa acatggagc gacatctcg tccccaatg cacaatgaa atccaggtc caaggctcc gttgccaaa gccctcgtc gataaccta gacccctac acaatgaat tacgctcaa gtttttgtc tacaacggc aaatacaac ggccccccg agcgaaacg ctcagtttc aagacacca gaaggcgtc cccgaggct atgtcctac ctcgaggct taccccctg ggggcgttc cggccgaac gatgccacg aaaaccgcc tcatccgcg tttctactc acttggaag aaaccggaa aaaccgaac gggatgttg accggttac gatatttac taccgccgg ttggacgac agtactacc aatagtaaa atcgcgagg aatccccaa atcaggggc ggggatgtc ctcagtgcg aagttaagt gggcttgaa cccgatacc aactatgtg atttacata gcggcaagg acaagtgcc gggccatca aaggagttt tatattgaa agaaggaca agtccgtct ggggctagc acgccccca ggagtcccg gactttgac tgggagacg gtcaaggcc gacgatgac gggaaagtc accgttagg attcaatgg aggccggat aaaaccgga aatcccggt tcgcacttc attgtgaag tacaagaga gaggcggaa ccgaattat gaagaagtc gaagtcctt aatgatgat tttgttgag attcccggg attgcccca agggagagt tatgacttt aaggtgact tcggtcgat ggtgactat tacgttgat agtaaggtc aaaactatc aatatttac tcggacggg cctttaatc aaggccaat gagaatatg gcgtcggca ggctggttt attgggatg atgcttgcc attgccatt ttgttgtta attttgatc gtcgtttgt attattaag cgcaaccgg ggcgggaag tacgccgtt catgagcgg gagcaagcc aatggccgg cacgattat cccgacgag ggcggtttt catgaatat tcccaaccg ttagacaat aagtcacat ggtagagct tcaatgagc agcgaacct aaagtaggt ccagagagt gataccgat tcaatggcc gaatacggt gacggagac acagagggt atgaatgag gacgggtca tttattggg caatatggc cgcaaacgt ggtcaggga gaaacaaca tcgcaaggg tttgcaact cttgtatag
- >TC001889 RA
gtccaaaat gaaataaaa atgaatcat tttcgttga agtataatg gacagtcaa atccttcgt ttagacatt tttgccata tgattaggt gactgaaaa tccggtgaa actcgcaga tctgaacca gtgatagtg aaaataagt gatttggtt agtgagtgt gacgaccgc ctagaccgc acctatctc tgttgtgtg tttgtagtg tagtgataa atcgttatc atggacaaa taacataga ttttgaaat aatctgggt gtactcgac acacacttc aaagatggc agatcgatt cattttaag tttgttagt gactgcgtt tagtttatc atgcacgct aggaaagga agcttcatc ccccccgta catcgtgaa acagccccc aaccgacga agtcctgtt ccaagtcga aagcggcgg cgaaaccga caagccctt ctacatcga atgcgaagc gataggcga acctgcacc aaaataccg ttgggtcaa aaacggtaa acccttcca ctggcaaac ctacgacga ccgcatatc gcaacagca aggccgtgg tacattatc aatttccaa accgaccga tgtcgacct cggccaata ccaatgttt cgccgaaaa cgagcacgg agtcgccac ctcgaactc cgtattcat gcgaaaagc cgaattgaa ctcgttcaa aacgacgca accgatttc actatcggg ggatgaggg cgaaccctt caaactgac gtgcgaacc acccgatgg ttggccgaa acctatagt gcactggat ttatctcta cacgaatgg aggattcaa aacgattaa tagctcgcg aatgacgat cgatcctga agggaattt gtggttttc gaatttgac gagggatga tgtcaccga taatttcaa gtatgcgtg tgcggcaac ctcaccctt caaacacga atacaagta tggaaacag cgtcttgct caatgttgt ctccaccgg agtctccgc cgcctctaa taagcggga accggtgtt acagtacgt ctcaaggaa aaatactgt agcctattt ggggaaaca acaaaaatt gtattgcat tttcggcgg gactccgct gccgcagac cgtgtggta caaagacgg caagccgat ccagtcttc ggaccgcat aactcagga taattacgg caaatcgct ggttatccg agtggtgaa cttcgaaga cgagggtaa atacacctg cgaagtgag taacggagt cggcgaagc gaaatcgta ttcgatcga tttgaaagt tttcgcgat tccctactt tatcgctga accggagcg tcaaaacgc cgccgaggg cgaaaccgt cgagtttcg gtgcgaggc cggcggtgt ccccaaacc cgaaatcaa gtggattca taatggaaa accgataag tgaggcacc acccaatcc gagacgacg agtcgagaa caatcgaat cgttatcga aagacttgt cgaaagcga taccggaaa ttacggatg taacgctac taattcttt gggttacgt gtacaagga cgtctacgt caatgttct ctctctagc acctgagat tctcgaagc tccgactga tacccaagc agtcgatgg ccaaaatgt cactatgat ttgtaaggt cttaggggc gcctaaacc ctcgattaa gtggagttt gaacgagcg ggaattgac aggtggacg ctacattgt ccagccaaa cggcgatct aatcatcag aaacgtcca gtttgacga caggggcaa ctacacctg tttcgccga aaataagtt cggcaacgc cagcgccaa agcggcact gctcgtcaa atcccacac atatatcac cgacggtcc tgaggacta cgaagtggc ggcgcacac ttcggccac ctttaggtg caacgcagt cagcgacga ttcgctaga cttggaaat aatttggct gaaaaacga ccagcctat cgacctaga ggaggaacc aagattcgt gcgctcgag tgattattc gttgaccat tactcaaac aattgagtt agattcagg gacttacac ttgcgtggc tcgaaccgc gttagacga agcgtctgc caaagctca acttattgt tcaagatgt accaaatcc gccctcgat ggagggagt cgagtgtca cgctaagga cgccaccat tcggtggaa gtcaatggg cgacaaccg ggcgccaat ttcgcacta tatcatcga atataacac cacgttcac tcctgaaac gtggactgc ggcttacaa taacgtgcc agcgactga tatgagcta caatgttcc tatgagccc atggactaa ttacacttt ccgagtaat tgccgttaa caaaatcgg aaaatcgtt accttccac ccattcaga tgtttgcac aactgagcc agatgtgcc ctacaagaa ccctgacga tgtcaaggg tgaaggtga cagacccga taatctcat tatttcgtg gacgccaat gccaaaaat cgaacataa cgccgacaa attccaata ccgcgtcta ctggaggcg tgacatacc aggggagcg ttacgaaag ccgcgacat taccgactg gagggttaa acaattcgt tgtcgataa tcaacccac gtttcaaca atatcgcat caaagtcgt tgcgatgaa cgagaaggg cgaagccaa cgtcgcacc gaaggaagt tataggcta ttcaggcga aaacatccc catcgaagc ccccacgaa cttcaccct tctccaaat ccaaggccc caagacggc gctccttag ctggaaccc cgttgacaa gaagagcgt ccaaggcca cttgaaagg ctacaaaat caaaacatg gagcgacat ctcgtcccc aatgcacaa tgaaatcca ggtccaagg ctccgttgc caaagccct cgtcgataa cctagaccc ctacacaat gaattacgc tcaagtttt tgtctacaa cggcaaata caacggccc cccgagcga aacgctcag tttcaagac accagaagg cgtccccga ggctatgtc ctacctcga ggcttaccc cctgggggc gttccggcc gaacgatgc cacgaaaac cgcctcatc cgcgtttct actcacttg gaagaaacc ggaaaaacc gaacgggat gttgaccgg ttacgatat ttactaccg ccggttgga cgacagtac taccaatag taaaatcgc gaggaatcc ccaaatcag gggcgggga tgtcctcag tgcgaagtt aagtgggct tgaacccga taccaacta tgtgattta catagcggc aaggacaag tgccgggcc atcaaagga gttttatat tgaaagaag gacaagtcc gtctggggc tagcacgcc cccaggagt cccggactt tgactggga gacggtcaa ggccgacga tgacgggaa agtcaccgt taggattca atggaggcc ggataaaac cggaaatcc cggttcgca cttcattgt gaagtacaa gagagaggc ggaaccgaa ttatgaaga agtcgaagt ccttaatga tgattttgt tgagattcc cgggattgc cccaaggga gagttatga ctttaaggt gacttcggt cgatggtga ctattacgt tgatagtaa ggtcaaaac tatcaatat ttactcgga cgggccttt aatcaaggc caatgagaa tatggcgtc ggcaggctg gtttattgg gatgatgct tgccattgc cattttgtt gttaatttt gatcgtcgt ttgtattat taagcgcaa ccggggcgg gaagtacgc cgttcatga gcgggagca agccaatgg ccggcacga ttatcccga cgagggcgg ttttcatga atattccca accgttaga caataagtc acatggtag agcttcaat gagcagcga acctaaagt aggtccaga gagtgatac cgattcaat ggccgaata cggtgacgg agacacaga gggtatgaa tgaggacgg gtcatttat tgggcaata tggccgcaa acgtggtca gggagaaac aacatcgca agggtttgc aactcttgt atagtaagg tttattttt tattttatt ttttatggg tggcttggt caaaataat tttctattg gctggtact aaatctcga tctgcttct gttttataa cacttttca atttccaat taaaatcgg ccagagtgg tgactgccg tggctaaaa atttgcaat ttcagggcg ttttactga agacggatc tttcatagg tcaatacgt tccaagcaa catgaagca gcttggccc accctctat tacggcagg ggttaacaa taatgcatt ggccacgta tgtttaaac gtgaccact tactctaaa ctaattctt taaacatat tttttgcta gagcagtgt tgtgacatt tttttagaa tttattttg tgtacttga tatggagaa agaatcgga aaaattaat tgactcact tattcgtgg aagatatta atttattgt ttgacaatg tgttctgat atttatttc gggtaaaaa ttgaattcg tccatttta ggttacgca taatttaaa aataaaaat caaaatgaa tttaatgta tagtaatat atttttgtt ttgcgtcac aatttggaa acgataggt actgaatgt ttgcaaatt gtaacgtgc tgatctgtt aagtactgg aataatttg tgtatcttt atgtattat atagttaat tataggatc gtatattta tgatgtttt tcatattat tgcccaagg aattacgtt ctcatataa tatataatg actataaat ttatgacat gtttcacgc ttgagaaat aattatttg taagttagg taataaaca tcaaaattg ctttaatca tcatttcct cgaattagt tttattctt gacactgcc taatttaag agatatcaa gtgtggcat taatttgga ctgtattag gcgtacaat aagtagtat ccacactaa gttactttt tatgaatcc gatcgaata agtaggcat cacagagtt catacaaag tgacttgac caaaactta ttgtacata actcgttta gaacctgct tatataaaa ataattcaa attaatcta agtaaagta agttaagct gaaatattt tttttacga ttttcgtag tttgtagta ttttgaaaa tttacattg caataattt accattacg tagattatt gccctgtta ttttttgta ttaagttaa gattaaggc tcaggccga gatatcatt aaattatga aagattttt cgtttttct ttttttgtg atcgaaaat gagacgtac attttctca aaaatggga acttgacac taaacaata atactgtgt caattcggc tttctgtaa aacaggaca tgggtgcta gtatcgtgg ttattaatt tgttgcatg attgttgct tgttatgtt tcaaataac agcttcatt tcttctctt gtgaggtaa aatggctaa atcaccaac ttacgaaat ggttttctt tgagagttt tgtagtttg gcttttgta agtgtaaaa tataaaatg ttttgtcgc cgagcacga attcttttg atatattgg cagaatgaa tgcactgtt ggatattag gtaagataa ttcgatata tttattatc aactattat aactgcttt actagttgt tctattata agatgatta tagtatttt tttaatatg tcttgtaca aaaagcatc aaaaacatt cagttgtgg ttttgtaat ggcagctgt aactttatt gtattataa atgggagta attgtaaat gtatgcttt caattttgt aagctgtat aaattcgta gtgttcttc cttcggtct caatagcct catactaca gatagtcca ctcgtatac tatataaac acttgatcg ttattattt ttatacact tttttaaac cattgtaaa tgccttcta tttaccctg agttgtaaa ttttttata tgatccctg ttttacaaa aat
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
