Status
Please wait ...
Gene information
General information
TC001889 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 569255..578689
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC001889
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_07748

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Fertility
    yolk late amount decreased - Number of animals showing the phenotype: 4

    vitellogenic egg chamber late amount increased - Number of animals showing the phenotype: 4
    mature egg not present - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    iBeetle screen iB_00309

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Fertility
    mature egg deposition blocked number 1st lateral oviduct: number 2nd lateral oviduct: - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001889 RA
      MADRFILSL LVTAFSLSC TLGKEASSP PYIVKQPPT 
DEVLFQVES GGETDKPFY IECEAIGEP APKYRWVKN 
GKPFHWQTY DDRISQQQG RGTLSISKP TDVDLGQYQ 
CFAENEHGV ATSNSVFMR KAELNSFKT TQPISLSGD 
EGEPFKLTC EPPDGWPKP IVHWIYLYT NGGFKTINS 
SRMTIDPEG NLWFSNLTR DDVTDNFKY ACAATSPFK 
HEYKYGNSV LLNVVSTGV SAASNKREP VLQYVSRKN 
TVAYLGKQQ KLYCIFGGT PLPQTVWYK DGKPIQSSD 
RITQDNYGK SLVIRVVNF EDEGKYTCE VSNGVGEAK 
SYSIDLKVF AIPYFIAEP ERQNAAEGE TVEFRCEAG 
GVPKPEIKW IHNGKPISE APPNPRRRV ENNRIVIER 
LVESDTGNY GCNATNSLG YVYKDVYVN VLSLAPEIL 
EAPTDTQAV DGQNVTMIC KVLGAPKPS IKWSLNERE 
LTGGRYIVQ PNGDLIIRN VQFDDRGNY TCFAENKFG 
NASAKAALL VKSHTYITD GPEDYEVAA HTSATFRCN 
AVSDDSLDL EIIWLKNDQ PIDLEEEPR FVRSSDYSL 
TITQTIELD SGTYTCVAR TALDEASAK AQLIVQDVP 
NPPSMEGVE CHAKDATIR WKSMGDNRA PISHYIIEY 
NTTFTPETW TAAYNNVPA TDMSYNVPM SPWTNYTFR 
VIAVNKIGK SLPSTHSDV CTTEPDVPY KNPDDVKGE 
GDRPDNLII SWTPMPKIE HNADKFQYR VYWRRDIPG 
ERYESRDIT DWRVKQFVV DNQPTFQQY RIKVVAMNE 
KGEANVAPK EVIGYSGEN IPIEAPTNF TLLQIQGPK 
TALLSWNPV DKKSVQGHL KGYKIKTWS DISSPMHNE 
IQVQGSVAK ALVDNLDPY TMNYAQVFV YNGKYNGPP 
SETLSFKTP EGVPEAMSY LEAYPLGAF RPNDATKTA 
SSAFLLTWK KPEKPNGML TGYDIYYRR LDDSTTNSK 
IARNPQIRG GDVLSAKLS GLEPDTNYV IYIAARTSA 
GPSKEFYIE RRTSPSGAS TPPGVPDFD WETVKADDD 
GKVTVRIQW RPDKTGNPG SHFIVKYKR EAEPNYEEV 
EVLNDDFVE IPGIAPRES YDFKVTSVD GDYYVDSKV 
KTINIYSDG PLIKANENM ASAGWFIGM MLAIAILLL 
ILIVVCIIK RNRGGKYAV HEREQANGR HDYPDEGGF 
HEYSQPLDN KSHGRASMS SEPKVGPES DTDSMAEYG 
DGDTEGMNE DGSFIGQYG RKRGQGETT SQGFATLV
    CDS sequence
    • >TC001889 RA
      atggcagat cgattcatt ttaagtttg ttagtgact 
gcgtttagt ttatcatgc acgctagga aaggaagct 
tcatccccc ccgtacatc gtgaaacag cccccaacc 
gacgaagtc ctgttccaa gtcgaaagc ggcggcgaa 
accgacaag cccttctac atcgaatgc gaagcgata 
ggcgaacct gcaccaaaa taccgttgg gtcaaaaac 
ggtaaaccc ttccactgg caaacctac gacgaccgc 
atatcgcaa cagcaaggc cgtggtaca ttatcaatt 
tccaaaccg accgatgtc gacctcggc caataccaa 
tgtttcgcc gaaaacgag cacggagtc gccacctcg 
aactccgta ttcatgcga aaagccgaa ttgaactcg 
ttcaaaacg acgcaaccg atttcacta tcgggggat 
gagggcgaa cccttcaaa ctgacgtgc gaaccaccc 
gatggttgg ccgaaacct atagtgcac tggatttat 
ctctacacg aatggagga ttcaaaacg attaatagc 
tcgcgaatg acgatcgat cctgaaggg aatttgtgg 
ttttcgaat ttgacgagg gatgatgtc accgataat 
ttcaagtat gcgtgtgcg gcaacctca cccttcaaa 
cacgaatac aagtatgga aacagcgtc ttgctcaat 
gttgtctcc accggagtc tccgccgcc tctaataag 
cgggaaccg gtgttacag tacgtctca aggaaaaat 
actgtagcc tatttgggg aaacaacaa aaattgtat 
tgcattttc ggcgggact ccgctgccg cagaccgtg 
tggtacaaa gacggcaag ccgatccag tcttcggac 
cgcataact caggataat tacggcaaa tcgctggtt 
atccgagtg gtgaacttc gaagacgag ggtaaatac 
acctgcgaa gtgagtaac ggagtcggc gaagcgaaa 
tcgtattcg atcgatttg aaagttttc gcgattccc 
tactttatc gctgaaccg gagcgtcaa aacgccgcc 
gagggcgaa accgtcgag tttcggtgc gaggccggc 
ggtgtcccc aaacccgaa atcaagtgg attcataat 
ggaaaaccg ataagtgag gcaccaccc aatccgaga 
cgacgagtc gagaacaat cgaatcgtt atcgaaaga 
cttgtcgaa agcgatacc ggaaattac ggatgtaac 
gctactaat tctttgggt tacgtgtac aaggacgtc 
tacgtcaat gttctctct ctagcacct gagattctc 
gaagctccg actgatacc caagcagtc gatggccaa 
aatgtcact atgatttgt aaggtctta ggggcgcct 
aaaccctcg attaagtgg agtttgaac gagcgggaa 
ttgacaggt ggacgctac attgtccag ccaaacggc 
gatctaatc atcagaaac gtccagttt gacgacagg 
ggcaactac acctgtttc gccgaaaat aagttcggc 
aacgccagc gccaaagcg gcactgctc gtcaaatcc 
cacacatat atcaccgac ggtcctgag gactacgaa 
gtggcggcg cacacttcg gccaccttt aggtgcaac 
gcagtcagc gacgattcg ctagacttg gaaataatt 
tggctgaaa aacgaccag cctatcgac ctagaggag 
gaaccaaga ttcgtgcgc tcgagtgat tattcgttg 
accattact caaacaatt gagttagat tcagggact 
tacacttgc gtggctcga accgcgtta gacgaagcg 
tctgccaaa gctcaactt attgttcaa gatgtacca 
aatccgccc tcgatggag ggagtcgag tgtcacgct 
aaggacgcc accattcgg tggaagtca atgggcgac 
aaccgggcg ccaatttcg cactatatc atcgaatat 
aacaccacg ttcactcct gaaacgtgg actgcggct 
tacaataac gtgccagcg actgatatg agctacaat 
gttcctatg agcccatgg actaattac actttccga 
gtaattgcc gttaacaaa atcggaaaa tcgttacct 
tccacccat tcagatgtt tgcacaact gagccagat 
gtgccctac aagaaccct gacgatgtc aagggtgaa 
ggtgacaga cccgataat ctcattatt tcgtggacg 
ccaatgcca aaaatcgaa cataacgcc gacaaattc 
caataccgc gtctactgg aggcgtgac ataccaggg 
gagcgttac gaaagccgc gacattacc gactggagg 
gttaaacaa ttcgttgtc gataatcaa cccacgttt 
caacaatat cgcatcaaa gtcgttgcg atgaacgag 
aagggcgaa gccaacgtc gcaccgaag gaagttata 
ggctattca ggcgaaaac atccccatc gaagccccc 
acgaacttc acccttctc caaatccaa ggccccaag 
acggcgctc cttagctgg aaccccgtt gacaagaag 
agcgtccaa ggccacttg aaaggctac aaaatcaaa 
acatggagc gacatctcg tccccaatg cacaatgaa 
atccaggtc caaggctcc gttgccaaa gccctcgtc 
gataaccta gacccctac acaatgaat tacgctcaa 
gtttttgtc tacaacggc aaatacaac ggccccccg 
agcgaaacg ctcagtttc aagacacca gaaggcgtc 
cccgaggct atgtcctac ctcgaggct taccccctg 
ggggcgttc cggccgaac gatgccacg aaaaccgcc 
tcatccgcg tttctactc acttggaag aaaccggaa 
aaaccgaac gggatgttg accggttac gatatttac 
taccgccgg ttggacgac agtactacc aatagtaaa 
atcgcgagg aatccccaa atcaggggc ggggatgtc 
ctcagtgcg aagttaagt gggcttgaa cccgatacc 
aactatgtg atttacata gcggcaagg acaagtgcc 
gggccatca aaggagttt tatattgaa agaaggaca 
agtccgtct ggggctagc acgccccca ggagtcccg 
gactttgac tgggagacg gtcaaggcc gacgatgac 
gggaaagtc accgttagg attcaatgg aggccggat 
aaaaccgga aatcccggt tcgcacttc attgtgaag 
tacaagaga gaggcggaa ccgaattat gaagaagtc 
gaagtcctt aatgatgat tttgttgag attcccggg 
attgcccca agggagagt tatgacttt aaggtgact 
tcggtcgat ggtgactat tacgttgat agtaaggtc 
aaaactatc aatatttac tcggacggg cctttaatc 
aaggccaat gagaatatg gcgtcggca ggctggttt 
attgggatg atgcttgcc attgccatt ttgttgtta 
attttgatc gtcgtttgt attattaag cgcaaccgg 
ggcgggaag tacgccgtt catgagcgg gagcaagcc 
aatggccgg cacgattat cccgacgag ggcggtttt 
catgaatat tcccaaccg ttagacaat aagtcacat 
ggtagagct tcaatgagc agcgaacct aaagtaggt 
ccagagagt gataccgat tcaatggcc gaatacggt 
gacggagac acagagggt atgaatgag gacgggtca 
tttattggg caatatggc cgcaaacgt ggtcaggga 
gaaacaaca tcgcaaggg tttgcaact cttgtatag
    mRNA sequence
    • >TC001889 RA
      gtccaaaat gaaataaaa atgaatcat tttcgttga 
agtataatg gacagtcaa atccttcgt ttagacatt 
tttgccata tgattaggt gactgaaaa tccggtgaa 
actcgcaga tctgaacca gtgatagtg aaaataagt 
gatttggtt agtgagtgt gacgaccgc ctagaccgc 
acctatctc tgttgtgtg tttgtagtg tagtgataa 
atcgttatc atggacaaa taacataga ttttgaaat 
aatctgggt gtactcgac acacacttc aaagatggc 
agatcgatt cattttaag tttgttagt gactgcgtt 
tagtttatc atgcacgct aggaaagga agcttcatc 
ccccccgta catcgtgaa acagccccc aaccgacga 
agtcctgtt ccaagtcga aagcggcgg cgaaaccga 
caagccctt ctacatcga atgcgaagc gataggcga 
acctgcacc aaaataccg ttgggtcaa aaacggtaa 
acccttcca ctggcaaac ctacgacga ccgcatatc 
gcaacagca aggccgtgg tacattatc aatttccaa 
accgaccga tgtcgacct cggccaata ccaatgttt 
cgccgaaaa cgagcacgg agtcgccac ctcgaactc 
cgtattcat gcgaaaagc cgaattgaa ctcgttcaa 
aacgacgca accgatttc actatcggg ggatgaggg 
cgaaccctt caaactgac gtgcgaacc acccgatgg 
ttggccgaa acctatagt gcactggat ttatctcta 
cacgaatgg aggattcaa aacgattaa tagctcgcg 
aatgacgat cgatcctga agggaattt gtggttttc 
gaatttgac gagggatga tgtcaccga taatttcaa 
gtatgcgtg tgcggcaac ctcaccctt caaacacga 
atacaagta tggaaacag cgtcttgct caatgttgt 
ctccaccgg agtctccgc cgcctctaa taagcggga 
accggtgtt acagtacgt ctcaaggaa aaatactgt 
agcctattt ggggaaaca acaaaaatt gtattgcat 
tttcggcgg gactccgct gccgcagac cgtgtggta 
caaagacgg caagccgat ccagtcttc ggaccgcat 
aactcagga taattacgg caaatcgct ggttatccg 
agtggtgaa cttcgaaga cgagggtaa atacacctg 
cgaagtgag taacggagt cggcgaagc gaaatcgta 
ttcgatcga tttgaaagt tttcgcgat tccctactt 
tatcgctga accggagcg tcaaaacgc cgccgaggg 
cgaaaccgt cgagtttcg gtgcgaggc cggcggtgt 
ccccaaacc cgaaatcaa gtggattca taatggaaa 
accgataag tgaggcacc acccaatcc gagacgacg 
agtcgagaa caatcgaat cgttatcga aagacttgt 
cgaaagcga taccggaaa ttacggatg taacgctac 
taattcttt gggttacgt gtacaagga cgtctacgt 
caatgttct ctctctagc acctgagat tctcgaagc 
tccgactga tacccaagc agtcgatgg ccaaaatgt 
cactatgat ttgtaaggt cttaggggc gcctaaacc 
ctcgattaa gtggagttt gaacgagcg ggaattgac 
aggtggacg ctacattgt ccagccaaa cggcgatct 
aatcatcag aaacgtcca gtttgacga caggggcaa 
ctacacctg tttcgccga aaataagtt cggcaacgc 
cagcgccaa agcggcact gctcgtcaa atcccacac 
atatatcac cgacggtcc tgaggacta cgaagtggc 
ggcgcacac ttcggccac ctttaggtg caacgcagt 
cagcgacga ttcgctaga cttggaaat aatttggct 
gaaaaacga ccagcctat cgacctaga ggaggaacc 
aagattcgt gcgctcgag tgattattc gttgaccat 
tactcaaac aattgagtt agattcagg gacttacac 
ttgcgtggc tcgaaccgc gttagacga agcgtctgc 
caaagctca acttattgt tcaagatgt accaaatcc 
gccctcgat ggagggagt cgagtgtca cgctaagga 
cgccaccat tcggtggaa gtcaatggg cgacaaccg 
ggcgccaat ttcgcacta tatcatcga atataacac 
cacgttcac tcctgaaac gtggactgc ggcttacaa 
taacgtgcc agcgactga tatgagcta caatgttcc 
tatgagccc atggactaa ttacacttt ccgagtaat 
tgccgttaa caaaatcgg aaaatcgtt accttccac 
ccattcaga tgtttgcac aactgagcc agatgtgcc 
ctacaagaa ccctgacga tgtcaaggg tgaaggtga 
cagacccga taatctcat tatttcgtg gacgccaat 
gccaaaaat cgaacataa cgccgacaa attccaata 
ccgcgtcta ctggaggcg tgacatacc aggggagcg 
ttacgaaag ccgcgacat taccgactg gagggttaa 
acaattcgt tgtcgataa tcaacccac gtttcaaca 
atatcgcat caaagtcgt tgcgatgaa cgagaaggg 
cgaagccaa cgtcgcacc gaaggaagt tataggcta 
ttcaggcga aaacatccc catcgaagc ccccacgaa 
cttcaccct tctccaaat ccaaggccc caagacggc 
gctccttag ctggaaccc cgttgacaa gaagagcgt 
ccaaggcca cttgaaagg ctacaaaat caaaacatg 
gagcgacat ctcgtcccc aatgcacaa tgaaatcca 
ggtccaagg ctccgttgc caaagccct cgtcgataa 
cctagaccc ctacacaat gaattacgc tcaagtttt 
tgtctacaa cggcaaata caacggccc cccgagcga 
aacgctcag tttcaagac accagaagg cgtccccga 
ggctatgtc ctacctcga ggcttaccc cctgggggc 
gttccggcc gaacgatgc cacgaaaac cgcctcatc 
cgcgtttct actcacttg gaagaaacc ggaaaaacc 
gaacgggat gttgaccgg ttacgatat ttactaccg 
ccggttgga cgacagtac taccaatag taaaatcgc 
gaggaatcc ccaaatcag gggcgggga tgtcctcag 
tgcgaagtt aagtgggct tgaacccga taccaacta 
tgtgattta catagcggc aaggacaag tgccgggcc 
atcaaagga gttttatat tgaaagaag gacaagtcc 
gtctggggc tagcacgcc cccaggagt cccggactt 
tgactggga gacggtcaa ggccgacga tgacgggaa 
agtcaccgt taggattca atggaggcc ggataaaac 
cggaaatcc cggttcgca cttcattgt gaagtacaa 
gagagaggc ggaaccgaa ttatgaaga agtcgaagt 
ccttaatga tgattttgt tgagattcc cgggattgc 
cccaaggga gagttatga ctttaaggt gacttcggt 
cgatggtga ctattacgt tgatagtaa ggtcaaaac 
tatcaatat ttactcgga cgggccttt aatcaaggc 
caatgagaa tatggcgtc ggcaggctg gtttattgg 
gatgatgct tgccattgc cattttgtt gttaatttt 
gatcgtcgt ttgtattat taagcgcaa ccggggcgg 
gaagtacgc cgttcatga gcgggagca agccaatgg 
ccggcacga ttatcccga cgagggcgg ttttcatga 
atattccca accgttaga caataagtc acatggtag 
agcttcaat gagcagcga acctaaagt aggtccaga 
gagtgatac cgattcaat ggccgaata cggtgacgg 
agacacaga gggtatgaa tgaggacgg gtcatttat 
tgggcaata tggccgcaa acgtggtca gggagaaac 
aacatcgca agggtttgc aactcttgt atagtaagg 
tttattttt tattttatt ttttatggg tggcttggt 
caaaataat tttctattg gctggtact aaatctcga 
tctgcttct gttttataa cacttttca atttccaat 
taaaatcgg ccagagtgg tgactgccg tggctaaaa 
atttgcaat ttcagggcg ttttactga agacggatc 
tttcatagg tcaatacgt tccaagcaa catgaagca 
gcttggccc accctctat tacggcagg ggttaacaa 
taatgcatt ggccacgta tgtttaaac gtgaccact 
tactctaaa ctaattctt taaacatat tttttgcta 
gagcagtgt tgtgacatt tttttagaa tttattttg 
tgtacttga tatggagaa agaatcgga aaaattaat 
tgactcact tattcgtgg aagatatta atttattgt 
ttgacaatg tgttctgat atttatttc gggtaaaaa 
ttgaattcg tccatttta ggttacgca taatttaaa 
aataaaaat caaaatgaa tttaatgta tagtaatat 
atttttgtt ttgcgtcac aatttggaa acgataggt 
actgaatgt ttgcaaatt gtaacgtgc tgatctgtt 
aagtactgg aataatttg tgtatcttt atgtattat 
atagttaat tataggatc gtatattta tgatgtttt 
tcatattat tgcccaagg aattacgtt ctcatataa 
tatataatg actataaat ttatgacat gtttcacgc 
ttgagaaat aattatttg taagttagg taataaaca 
tcaaaattg ctttaatca tcatttcct cgaattagt 
tttattctt gacactgcc taatttaag agatatcaa 
gtgtggcat taatttgga ctgtattag gcgtacaat 
aagtagtat ccacactaa gttactttt tatgaatcc 
gatcgaata agtaggcat cacagagtt catacaaag 
tgacttgac caaaactta ttgtacata actcgttta 
gaacctgct tatataaaa ataattcaa attaatcta 
agtaaagta agttaagct gaaatattt tttttacga 
ttttcgtag tttgtagta ttttgaaaa tttacattg 
caataattt accattacg tagattatt gccctgtta 
ttttttgta ttaagttaa gattaaggc tcaggccga 
gatatcatt aaattatga aagattttt cgtttttct 
ttttttgtg atcgaaaat gagacgtac attttctca 
aaaatggga acttgacac taaacaata atactgtgt 
caattcggc tttctgtaa aacaggaca tgggtgcta 
gtatcgtgg ttattaatt tgttgcatg attgttgct 
tgttatgtt tcaaataac agcttcatt tcttctctt 
gtgaggtaa aatggctaa atcaccaac ttacgaaat 
ggttttctt tgagagttt tgtagtttg gcttttgta 
agtgtaaaa tataaaatg ttttgtcgc cgagcacga 
attcttttg atatattgg cagaatgaa tgcactgtt 
ggatattag gtaagataa ttcgatata tttattatc 
aactattat aactgcttt actagttgt tctattata 
agatgatta tagtatttt tttaatatg tcttgtaca 
aaaagcatc aaaaacatt cagttgtgg ttttgtaat 
ggcagctgt aactttatt gtattataa atgggagta 
attgtaaat gtatgcttt caattttgt aagctgtat 
aaattcgta gtgttcttc cttcggtct caatagcct 
catactaca gatagtcca ctcgtatac tatataaac 
acttgatcg ttattattt ttatacact tttttaaac 
cattgtaaa tgccttcta tttaccctg agttgtaaa 
ttttttata tgatccctg ttttacaaa aat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY