Status
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Gene information
General information
TC008551 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451492.1: 286325..291798
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008551
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_07746

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008551 RA
      MGDLSEYIK NHLYLLHKK KSDLFGTVQ ERVMKMNTQ 
      RKQSRLDDA LVKRRIPEN MSPIVTPVP KRNKREERR 
      EPADPGVNK RLEMLKKWK TEKEKRKEV QKKTSKPIF 
      KVSHVSVAL GLPKLEMVN REIKGKPFK SKFAPPNHK 
      FHPPPNIQP IYFDNKDPQ SSKNTSRDK KTEKESHKM 
      VTRSQARAT SALQKNEAK VKTTGKKTP TKASRMGEK 
      NKTREDSDL TLVPPKQPV TKAKSRAKT EKSNTTKEK 
      NVKHENSDA PMKTPVLTK SEPSSTVQQ DKIEETNEN 
      KENIDLSLI SPDYPVANI QSTESDEVQ TPIKVAPPT 
      YISPFVTIS RGKNSARKE YVVRKLGQS FSSNIDTPD 
      YTSPKAGAV YFTWLLDSE ISRLKDMCD QWTTYKNEM 
      DPPEEAVSM IDVALGQTN LLITKKFEQ FRGLIHKCQ 
      SEPEEALVT CEDLHGFWD MMHLQVNNL DKRFANLDN 
      LKANNWQEI IPEVKKDVK KKSRKPKTV KASSALREA 
      IQAARKKKQ ENPDISLNK SNKRVSVMQ SEEKKKRIS 
      SPGLLMMKV AQFGKNVET PAKSILKET SVKSNQKSQ 
      QKSVLFHDE VSVEIRDTD LHNSGVKTP IRRSERLSK 
      GKLF
    CDS sequence
    • >TC008551 RA
      atgggggat ttatctgaa tatataaaa aaccatctc 
      taccttctt cataaaaag aaaagtgac ttgtttggt 
      acagtgcaa gagcgggtc atgaaaatg aacacgcaa 
      agaaaacaa agccgcttg gacgacgcc ttggtcaaa 
      cgaagaatt cccgagaac atgtctcca atcgtaaca 
      ccagtcccc aaaagaaat aaacgagaa gaaagacgt 
      gagccagca gatccagga gtgaacaaa cggctggag 
      atgctaaaa aaatggaag acagagaag gagaaacgg 
      aaagaagtg cagaaaaag accagcaaa cccattttc 
      aaagtgtcg catgtctct gtggctctg gggcttccc 
      aaactggag atggtgaat agggaaatc aaaggcaaa 
      ccgttcaag tcgaagttc gctcctccg aatcataaa 
      tttcacccg ccaccgaac atacaacct atttatttt 
      gataacaaa gacccacag tcgtcgaaa aatacgagt 
      agagataaa aagactgaa aaagagtct cacaaaatg 
      gtaacgagg tcgcaggcg cgggctacg tcggcactt 
      cagaaaaac gaagccaaa gtgaagaca acgggaaaa 
      aagactcct accaaagcg agcagaatg ggcgagaag 
      aataaaacc agagaagat agtgattta actttagta 
      ccacctaag cagccagta accaaagcg aaatcaaga 
      gcaaaaact gaaaaatcc aacacaacg aaggagaag 
      aatgtaaaa cacgaaaac agtgatgca cctatgaaa 
      acgcctgtc ttaacgaaa tcagagcca agttccact 
      gttcaacag gacaaaata gaggaaacg aatgaaaat 
      aaagaaaat attgactta tctttgata tcacctgac 
      tacccagtt gcgaatatt caatcaact gaaagtgat 
      gaggtgcag acgcctatt aaagtggcg cctcctacg 
      tacattagc cctttcgtg acaatatct cgaggaaaa 
      aacagtgct cgaaaagag tacgtagtt cgaaaatta 
      ggacaatct ttttcttca aatatagac actcctgat 
      tacacgtcg ccaaaagca ggcgctgtc tatttcacg 
      tggcttttg gatagtgaa atatctcgt ttgaaagac 
      atgtgcgac caatggact acatataaa aatgaaatg 
      gatcctcct gaggaagcc gtctcaatg attgacgtt 
      gccttgggc caaactaat ctattaata acaaaaaaa 
      ttcgagcaa ttccgaggt ttgatacac aagtgtcaa 
      tcagaacct gaagaggct cttgtcaca tgtgaagat 
      ctacacggt ttttgggat atgatgcat ttacaagtc 
      aataatttg gacaagaga ttcgcaaac ctagacaat 
      ttgaaagcc aacaattgg caagaaatt atacctgaa 
      gtgaagaag gacgtgaaa aagaagagt cgaaaaccg 
      aaaacagtg aaagcttca agtgctctt cgagaagct 
      attcaagct gctcgcaaa aagaaacaa gaaaatcca 
      gacatttcg ctcaataaa agtaataaa agagtctca 
      gttatgcaa agcgaagag aaaaagaaa agaattagt 
      tctccggga cttttgatg atgaaagtg gcccaattt 
      gggaaaaat gtcgagact cctgccaaa agtatattg 
      aaagaaaca tctgtgaaa tcaaatcag aaaagtcaa 
      caaaaatct gtgttgttt catgatgaa gtatcagtc 
      gaaattagg gacactgat ctacacaat tctggagtg 
      aaaacacca atacggcgg tcagaaaga ctttcgaaa 
      ggtaaattg ttttaa
    mRNA sequence
    • >TC008551 RA
      ggtaggtag actagctaa ttttgacag tacttgctt 
      aaattaacg gtcatagaa atttgattt tgtgaattg 
      tatgtttgt aacgtttgt cgtttggtg ttacatgtt 
      atttaaaac gcgcttgtg ttgtgtgtt ttcttaaaa 
      gtacaattt aatttacac attttcgag tagtcagtg 
      ttacaaaat gtaatgggg gatttatct gaatatata 
      aaaaaccat ctctacctt cttcataaa aagaaaagt 
      gacttgttt ggtacagtg caagagcgg gtcatgaaa 
      atgaacacg caaagaaaa caaagccgc ttggacgac 
      gccttggtc aaacgaaga attcccgag aacatgtct 
      ccaatcgta acaccagtc cccaaaaga aataaacga 
      gaagaaaga cgtgagcca gcagatcca ggagtgaac 
      aaacggctg gagatgcta aaaaaatgg aagacagag 
      aaggagaaa cggaaagaa gtgcagaaa aagaccagc 
      aaacccatt ttcaaagtg tcgcatgtc tctgtggct 
      ctggggctt cccaaactg gagatggtg aatagggaa 
      atcaaaggc aaaccgttc aagtcgaag ttcgctcct 
      ccgaatcat aaatttcac ccgccaccg aacatacaa 
      cctatttat tttgataac aaagaccca cagtcgtcg 
      aaaaatacg agtagagat aaaaagact gaaaaagag 
      tctcacaaa atggtaacg aggtcgcag gcgcgggct 
      acgtcggca cttcagaaa aacgaagcc aaagtgaag 
      acaacggga aaaaagact cctaccaaa gcgagcaga 
      atgggcgag aagaataaa accagagaa gatagtgat 
      ttaacttta gtaccacct aagcagcca gtaaccaaa 
      gcgaaatca agagcaaaa actgaaaaa tccaacaca 
      acgaaggag aagaatgta aaacacgaa aacagtgat 
      gcacctatg aaaacgcct gtcttaacg aaatcagag 
      ccaagttcc actgttcaa caggacaaa atagaggaa 
      acgaatgaa aataaagaa aatattgac ttatctttg 
      atatcacct gactaccca gttgcgaat attcaatca 
      actgaaagt gatgaggtg cagacgcct attaaagtg 
      gcgcctcct acgtacatt agccctttc gtgacaata 
      tctcgagga aaaaacagt gctcgaaaa gagtacgta 
      gttcgaaaa ttaggacaa tctttttct tcaaatata 
      gacactcct gattacacg tcgccaaaa gcaggcgct 
      gtctatttc acgtggctt ttggatagt gaaatatct 
      cgtttgaaa gacatgtgc gaccaatgg actacatat 
      aaaaatgaa atggatcct cctgaggaa gccgtctca 
      atgattgac gttgccttg ggccaaact aatctatta 
      ataacaaaa aaattcgag caattccga ggtttgata 
      cacaagtgt caatcagaa cctgaagag gctcttgtc 
      acatgtgaa gatctacac ggtttttgg gatatgatg 
      catttacaa gtcaataat ttggacaag agattcgca 
      aacctagac aatttgaaa gccaacaat tggcaagaa 
      attatacct gaagtgaag aaggacgtg aaaaagaag 
      agtcgaaaa ccgaaaaca gtgaaagct tcaagtgct 
      cttcgagaa gctattcaa gctgctcgc aaaaagaaa 
      caagaaaat ccagacatt tcgctcaat aaaagtaat 
      aaaagagtc tcagttatg caaagcgaa gagaaaaag 
      aaaagaatt agttctccg ggacttttg atgatgaaa 
      gtggcccaa tttgggaaa aatgtcgag actcctgcc 
      aaaagtata ttgaaagaa acatctgtg aaatcaaat 
      cagaaaagt caacaaaaa tctgtgttg tttcatgat 
      gaagtatca gtcgaaatt agggacact gatctacac 
      aattctgga gtgaaaaca ccaatacgg cggtcagaa 
      agactttcg aaaggtaaa ttgttttaa aatgttaac 
      tgataattt tttgtgtgt ttaaaagtt ggtaatttt 
      tataaatga attgtttta attgtgtta atattgcag 
      taataaagg taatgactt cctgccgtt tgtttcctt 
      gcttaccta cagtttttt tccatgtag ccccgtagt 
      cattatttt tttttttgg gagtatgta ttattagca 
      ctttttttg ttcaagaaa aaaagaaag taagagtga 
      tttctattg agttgttta tttccagtt tatcaaatt 
      acatattaa agcattaga acatgcagt gactaagaa 
      attacgaaa ctaattttt gtacaatta atcaattaa 
      ttgaatagt aactaaaac cactacgtt gcatgattc 
      aaaacaata ttacattta ttatgtaaa actgtaaac 
      cacacaatc tattctaaa tgcatatat ttttacatt 
      aaacttaaa aaaatatac gtatataca atctaatgg 
      cacccttag actacgtat aggtttaca atataccac 
      tgcccagtt ttttaaact agacaaata ctgttagtt 
      gctttttga tttgtatga ttggaaaaa tgtgatttg 
      cattgcttg tttatttat ggttgtgaa aagtgtgag 
      ccaaacgga tgggtaaat gaacagata atgaaaaga 
      caattgttg gtgtaccca tttttataa aaaagcgaa 
      aactaagtg aatagaaaa taaaatcga aatttaaaa 
      aagatcaaa aaaatggag aaaaacaag caaatcaaa 
      tataaagaa gaacgcaaa ttagtgatt ttttttctt 
      acactaatt tgcatttga agcaattta agtccagaa 
      atggttgtt gacgaaaaa tgtttaaag tttttgaag 
      aaaaacaaa gatcaaata aaattggac aaacacata 
      aaacaaaaa agatgaaga taaaaatgg ccttcaaga 
      aataaatct agaagaaaa atcagtgct gaaccactg 
      aagagttcg aaattaatt taaaaaatc aaaaaattg 
      ttccattga aacaaataa agttctaat aaagacaga 
      agagatcaa agaacaaag agaagtggt ggaagcggt 
      agtattgtt gaaatgcta atgcatatt gaagatttt 
      gtgaaacta atcaaaaaa tttaacgtc tagagtttg 
      atagatcag aaagtggaa aaaccgaag acagagaca 
      ggaacaggg tgttccatc taaacgtcg catgaaagc 
      actaagatt tttaatttg ggtgtttga aagaaagtt 
      tagatattc cc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY