Status
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Gene information
General information
TC002335 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015452276.1: 254681..256734
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002335
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_07744

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002335 RA
      MDRLSPQPG GCPLVHLGH TSSDCNESF ERKQYYQPS 
      SPLDLSSLS LGSGYWGYN DAPKYYRAP SNYLLDNFT 
      KLGKSSPSL DQGYHTLGF APPEGNIFK GKKYQNKNN 
      SFDRLTDEV IIRIFSFLS SIDLSICAM VCRRFNILA 
      WVPPLWRII RLEGEHVRG DRAIRGILR QLCGQMDTC 
      PNIERIHVT FGAKISDKS LLMLARRCP ELTHLQLIG 
      CTVTNNALF ELVTRCTNL QHLNVTGCV KISCISINP 
      GPDSSRRLQ LQYLDLTDC SALQDSGLR VIVHNCPQL 
      THLYLRRCV QITDAGLKF VPSFCTDLK ELSVSDCVN 
      ITDFGLYEL GKLGPVLRY LSVAKCHQV SDAGLKVIA 
      RRCYKLRYL NARGCEAVS DDAVIFLAR SCTRLCALD 
      IGKCDVSDA GLRALAESC PNLKKLSLR SCDLVTDRG 
      VQCVAYFCR GLQQLNIQD CQITLEGYR AVKKYCKRC 
      VIEHTNPGF F
    CDS sequence
    • >TC002335 RA
      atggaccgt ctctcgccg caacccggg ggctgtcct 
      ctcgtccac ctggggcac acctcatcc gactgcaac 
      gagagcttc gaacgcaaa cagtactac cagcccagc 
      agccccctt gacctctcg tccttgtca ctcgggagc 
      ggctactgg ggctacaac gacgctccc aagtactac 
      cgcgccccc tcgaattac ctccttgat aatttcacc 
      aagttgggc aagtccagc cccagtctt gaccagggg 
      taccacacc ctgggtttc gccccgccc gaaggcaac 
      atattcaaa ggaaagaag taccagaac aagaacaat 
      tcgttcgac cgcttgacc gatgaagta ataatccgg 
      attttttcg tttttgtca agcattgat ttgagtatt 
      tgtgccatg gtttgccgc cgctttaac atcctagcg 
      tgggtaccc cctttgtgg cggatcatc aggcttgag 
      ggggagcac gtccggggg gaccgggcc ataaggggc 
      atcctgcgc cagctctgc ggccagatg gacacctgc 
      cccaacatc gagcgcatc catgtgact ttcggggcg 
      aaaatctcc gacaagagc ctcctgatg ttggcccgc 
      aggtgcccc gaactgacc cacttgcaa ctgatcggt 
      tgcacggtc accaacaac gctctcttc gagctcgtc 
      acacgctgc acaaacttg caacacttg aacgtaaca 
      ggttgtgtg aaaatctcc tgcattagc ataaatcca 
      gggccggac tcgtcgcgc cgcctccag ctccaatat 
      ctggacctg accgactgc tccgccctc caggacagc 
      ggcttgcgg gtgattgtg cacaactgc ccccagctg 
      acccacctg tacctccgc cgctgtgtg caaatcacg 
      gacgcgggg ctcaaattc gtgcccagt ttctgcacc 
      gatttgaag gagctgagt gtgagcgac tgtgtcaac 
      atcaccgat ttcggactg tacgagctg ggcaagctc 
      ggccccgtc ttgcgctac ttgtcggtg gcgaagtgc 
      caccaagtg agcgacgcg gggctcaag gtcatagcc 
      agaaggtgc tacaagttg aggtatttg aacgcgagg 
      ggctgcgag gctgtctct gacgacgct gttattttc 
      ctggcgagg tcgtgcacc aggttgtgc gcgctggat 
      atcgggaag tgcgacgtt agcgatgcc gggctgagg 
      gctctggcg gagagctgt cccaatctg aagaagctg 
      agtctgagg agttgcgat cttgtgacg gatcggggg 
      gtgcaatgc gtggcgtat ttctgcagg ggactgcag 
      cagctcaat atacaggat tgccagatt acgttagag 
      gggtacagg gctgttaag aagtattgc aagcggtgt 
      gtgattgaa cacaccaat ccgggattt ttctaa
    mRNA sequence
    • >TC002335 RA
      cacgaacca tggaacagc atagaataa gaacccgca 
      atccatcgg tttgaacca aaatgtttt gcgtcgaat 
      tctttgagt ttccgtttc cttttttcc agtagtcaa 
      aacacaact agtggtaca ccaaagaaa acactaaac 
      tatcacatc aaacttcaa tattttgtc cagttcgtc 
      cagttttta cacaactaa gaattatta acgcaactt 
      cactgaaga aaatttgta aatgaacaa aatttcttt 
      gcgcgattg gcacccctg cgcacaatt gacaaaaag 
      tgacaactt tgacattta tttaggtta taacaattg 
      catttgcaa acctcaatt ccgcccatt tcctgccat 
      aaacccgca atggggacc ccagaatga ctgttcctc 
      cactccgga gcactcatc aactcaact taccgtgcc 
      acaaaccct atgaagact aattgagcc cccgttggc 
      tgggccatg gaccgtctc tcgccgcaa cccgggggc 
      tgtcctctc gtccacctg gggcacacc tcatccgac 
      tgcaacgag agcttcgaa cgcaaacag tactaccag 
      cccagcagc ccccttgac ctctcgtcc ttgtcactc 
      gggagcggc tactggggc tacaacgac gctcccaag 
      tactaccgc gccccctcg aattacctc cttgataat 
      ttcaccaag ttgggcaag tccagcccc agtcttgac 
      caggggtac cacaccctg ggtttcgcc ccgcccgaa 
      ggcaacata ttcaaagga aagaagtac cagaacaag 
      aacaattcg ttcgaccgc ttgaccgat gaagtaata 
      atccggatt ttttcgttt ttgtcaagc attgatttg 
      agtatttgt gccatggtt tgccgccgc tttaacatc 
      ctagcgtgg gtaccccct ttgtggcgg atcatcagg 
      cttgagggg gagcacgtc cggggggac cgggccata 
      aggggcatc ctgcgccag ctctgcggc cagatggac 
      acctgcccc aacatcgag cgcatccat gtgactttc 
      ggggcgaaa atctccgac aagagcctc ctgatgttg 
      gcccgcagg tgccccgaa ctgacccac ttgcaactg 
      atcggttgc acggtcacc aacaacgct ctcttcgag 
      ctcgtcaca cgctgcaca aacttgcaa cacttgaac 
      gtaacaggt tgtgtgaaa atctcctgc attagcata 
      aatccaggg ccggactcg tcgcgccgc ctccagctc 
      caatatctg gacctgacc gactgctcc gccctccag 
      gacagcggc ttgcgggtg attgtgcac aactgcccc 
      cagctgacc cacctgtac ctccgccgc tgtgtgcaa 
      atcacggac gcggggctc aaattcgtg cccagtttc 
      tgcaccgat ttgaaggag ctgagtgtg agcgactgt 
      gtcaacatc accgatttc ggactgtac gagctgggc 
      aagctcggc cccgtcttg cgctacttg tcggtggcg 
      aagtgccac caagtgagc gacgcgggg ctcaaggtc 
      atagccaga aggtgctac aagttgagg tatttgaac 
      gcgaggggc tgcgaggct gtctctgac gacgctgtt 
      attttcctg gcgaggtcg tgcaccagg ttgtgcgcg 
      ctggatatc gggaagtgc gacgttagc gatgccggg 
      ctgagggct ctggcggag agctgtccc aatctgaag 
      aagctgagt ctgaggagt tgcgatctt gtgacggat 
      cggggggtg caatgcgtg gcgtatttc tgcagggga 
      ctgcagcag ctcaatata caggattgc cagattacg 
      ttagagggg tacagggct gttaagaag tattgcaag 
      cggtgtgtg attgaacac accaatccg ggatttttc 
      taagccatt ttatgtatt tttatattg agattgtta 
      actatttat ttttgtata tttgtatat gctaagaat 
      attttttat taaattttt tactctact
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY