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Gene information
General information
TC006850 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450974.1: 152002..164029
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006850
    iBeetle screen iB_07737

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006850 RA
      MSSPIQSST LDGLPKQFV AAMRTLFDI MDDKRTGLV 
      NFADIENRW QDDGSKGLP KGVLESLRK VTPPNGMLT 
      FERFCAGLK ICLLRNQVE RRSDKREGA APTNRPPSA 
      PLLDIQEPP KQNWNTAAI RPNNVMSQQ RTISMPQLL 
      PKDPQGEKP VPNINKPLY GPPKPPRSA IGIDRGGNM 
      DRAEIRTAL QNWQMGLMM NDQENKTEK RPQYTGLLR 
      TGRSLGDGK AAQSDIPVA PGMYQKKAN VRRREPRRH 
      TLQNGIDYN MLKRMKQIE QEKDVLMQG LNAVEKARE 
      WYLKQVAAV QEKMKYLGR MGHVEPWSE AQQERLELQ 
      RARVFEVNR HLAALTDSW ERGGLPLHM NLAVHHYPQ 
      NLDLNDRLK QQNRLLTEE VSKKSERIS LLEREKANL 
      IQMLQMRSA PNRNGQEEA VF
    CDS sequence
    • >TC006850 RA
      atgtcatcg cccatccag tcgtcaacc ctcgacggc 
      ctccccaag cagttcgtg gctgccatg cgcaccctc 
      ttcgacatc atggacgac aaaaggacg ggtttggtc 
      aattttgcg gacatcgag aacagatgg caagacgac 
      ggctccaaa ggcttgccc aagggcgtc ctcgagagc 
      ctgcgcaag gtgacaccc cccaatgga atgctcacc 
      ttcgagagg ttctgtgcg gggttgaaa atctgcctg 
      ctgaggaac caagtggag aggaggagc gacaagagg 
      gagggggct gcgcccacc aacagaccc ccgtcagca 
      ccgcttttg gacatacag gagcccccg aaacaaaac 
      tggaacact gccgccatc aggccaaac aacgtcatg 
      tcgcaacag cggacgata agtatgccc caactcctc 
      cccaaggac ccccagggg gagaagccc gtccccaac 
      atcaacaag ccgctctac ggcccccca aagcccccc 
      agaagcgcc atcggcatc gaccgaggg ggcaacatg 
      gacagggct gagatccgg acggcgctc caaaactgg 
      caaatgggc ctgatgatg aacgaccag gagaacaaa 
      accgagaaa cggccccaa tacactggc ctcctccgc 
      actggccga agtctaggt gatggcaaa gccgcccag 
      agtgatatt ccagtcgcc cccgggatg tatcagaag 
      aaagcaaac gttagacga agagaaccg agacgacac 
      actttgcag aacggaatc gattacaat atgttgaag 
      cggatgaag cagatagag caagaaaag gacgttttg 
      atgcaaggg ttgaatgcg gtggagaag gcgcgagag 
      tggtatttg aagcaagtt gcagcggtg caagaaaaa 
      atgaagtat ttaggacgg atgggtcac gtggaaccg 
      tggtcggaa gcccagcag gaaagactg gagctacaa 
      agggctcga gtttttgaa gttaaccga catttagcc 
      gccctcaca gacagttgg gaacgtgga ggtcttccc 
      ctacatatg aacctggct gttcatcat tacccgcaa 
      aatctagac cttaacgat cgactcaag cagcaaaat 
      cggctccta actgaggag gtgagcaaa aaaagtgaa 
      aggatatcg cttttggag cgggaaaag gcgaatctg 
      attcaaatg cttcaaatg aggtcagcg cccaatagg 
      aacggacaa gaagaggcg gttttttga
    mRNA sequence
    • >TC006850 RA
      caatctcag tcgagttca gccgaagca cggtaccac 
      tcgtgcggt cgatggtac tgctaacaa aagtgtaaa 
      caacacaac atgtcatcg cccatccag tcgtcaacc 
      ctcgacggc ctccccaag cagttcgtg gctgccatg 
      cgcaccctc ttcgacatc atggacgac aaaaggacg 
      ggtttggtc aattttgcg gacatcgag aacagatgg 
      caagacgac ggctccaaa ggcttgccc aagggcgtc 
      ctcgagagc ctgcgcaag gtgacaccc cccaatgga 
      atgctcacc ttcgagagg ttctgtgcg gggttgaaa 
      atctgcctg ctgaggaac caagtggag aggaggagc 
      gacaagagg gagggggct gcgcccacc aacagaccc 
      ccgtcagca ccgcttttg gacatacag gagcccccg 
      aaacaaaac tggaacact gccgccatc aggccaaac 
      aacgtcatg tcgcaacag cggacgata agtatgccc 
      caactcctc cccaaggac ccccagggg gagaagccc 
      gtccccaac atcaacaag ccgctctac ggcccccca 
      aagcccccc agaagcgcc atcggcatc gaccgaggg 
      ggcaacatg gacagggct gagatccgg acggcgctc 
      caaaactgg caaatgggc ctgatgatg aacgaccag 
      gagaacaaa accgagaaa cggccccaa tacactggc 
      ctcctccgc actggccga agtctaggt gatggcaaa 
      gccgcccag agtgatatt ccagtcgcc cccgggatg 
      tatcagaag aaagcaaac gttagacga agagaaccg 
      agacgacac actttgcag aacggaatc gattacaat 
      atgttgaag cggatgaag cagatagag caagaaaag 
      gacgttttg atgcaaggg ttgaatgcg gtggagaag 
      gcgcgagag tggtatttg aagcaagtt gcagcggtg 
      caagaaaaa atgaagtat ttaggacgg atgggtcac 
      gtggaaccg tggtcggaa gcccagcag gaaagactg 
      gagctacaa agggctcga gtttttgaa gttaaccga 
      catttagcc gccctcaca gacagttgg gaacgtgga 
      ggtcttccc ctacatatg aacctggct gttcatcat 
      tacccgcaa aatctagac cttaacgat cgactcaag 
      cagcaaaat cggctccta actgaggag gtgagcaaa 
      aaaagtgaa aggatatcg cttttggag cgggaaaag 
      gcgaatctg attcaaatg cttcaaatg aggtcagcg 
      cccaatagg aacggacaa gaagaggcg gttttttga 
      aaaatcaaa tcacttttt atcaaaaca catttatta 
      acagtcatt gaaacaatt gtgattctt ttgtgaaaa 
      tataaaacg taacggtgt tacctacgt gttatacat 
      agatatgtt tcttagata acttgtacc aggaagtgc 
      cattaacat acaatttgt tcgtaatta agttaaggt 
      tggtacatt ttatattca actgccaaa ttgtgggtg 
      attttgtgc aaattttta aactggtgc tagccattt 
      ttcatatac tggctggcc caaaactca aacatgtca 
      cagttttgg gtcacacag tatgcaaac atttactaa 
      taaagtata tcatttttt gtatgtctt ggtttcatt 
      tggccataa atatagggg agaacggca cagtgaata 
      cttaaacaa agtatgttc ctgattaaa caattcgtt 
      taataaaaa aaatgtgac caaataact accactcgt 
      aaaggaact ttattcaaa actgtcgcc tttttttaa 
      ataaaaacc aacaaattc tcacacttt ttttcaaaa 
      tcgtctcac tttcatttc tcaagcata aaaaatagt 
      ttaaatcat tctaaaata ataattata atttaaata 
      aaaattatt attatga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY