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Gene information
General information
TC016413 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450728.1: 80883..87959
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC016413
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
      {{ blurIdResult }}
      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
      {{ blurEvidenceResult }}
      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
      {{ blurReferenceResult }}
      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
      You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
      Email
      We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
      Publish name
      If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_07725

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      Fertility
      mature egg deposition blocked number 1st lateral oviduct: number 2nd lateral oviduct: - Number of animals showing the phenotype: 2
      ovary without detectable defects - Number of animals showing the phenotype: 2

      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 50
      embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC016413 RA
        MRKKDKTPL PLVIVELPK HETNIYNIT TCVSLDVRI 
        EPLKKTNQI GQCFNCQSY GHSQRNCHA TAKCLKCAE 
        THPSQECPK PKTTTAKCT NYNGPHPAS YRGCPVYPP 
        NQQMRAIKL TSNPWTKNT TQVDQSSPP SALNTENFP 
        PLKPKAWNA NGLHNKLNE LHQFTLLNN IDIMLINET 
        HAKPSDTLK IPNYIQYRT DRQTHRGCG TAIYLKQNL 
        KHHQPSQIT TENIENTSI VFQTKNGPL QITSAYKPP 
        HKDLISTDL HKLIKTKIP TLIAGNLNC KQRSWNSLN 
        NQNEQTLLE YTYQNNCLI LGPTKPTHF GAHSAPDVL 
        DIAVIKDIT LAIEIFTIN ELSSDYNPI IIELNTTSR 
        LTPEIKKIT FWPLFSKRV ETKTKIKTI NTAQELEDE 
        TKTRPSQTL YIDQQFSPN YQLINENIY RHIKKISKI 
        IPSLAQTDP PLTPISPEE IKSIISKAS NKKSPGPDK 
        IPKKPLKLL PHKTIIKFT NILNAILRL RHLPQSWKH 
        AHIILIPKP GKDPTFPQN YRPINLLSN VSKISERTI 
        LNRLTEELN HNQTLPNEQ FGFRSHHDT TPQLLRVTE 
        RITNNFNNK NKTAMICFD IEKAFDKVW HPGIIAKLC 
        KANLNFRLT EPIMSFLKN RTFQVKSQN QLSNTKKCK 
        AGVPQEAIL SPTLFNIFT HDIPKHTQT EIALFADDI 
        TIMTTSSK
      • >TC016413 RB
        MRAIKLTSN PWTKNTTQV DQSSPPSAL NTENFPPLK 
        PKAWNANGL HNKLNELHQ FTLLNNIDI MLINETHAK 
        PSDTLKIPN YIQYRTDRQ THRGCGTAI YLKQNLKHH 
        QPSQITTEN IENTSIVFQ TKNGPLQIT SAYKPPHKD 
        LISTDLHKL IKTKIPTLI AGNLNCKQR SWNSLNNQN 
        EQTLLEYTY QNNCLILGP TKPTHFGAH SAPDVLDIA 
        VIKDITLAI EIFTINELS SDYNPIIIE LNTTSRLTP 
        EIKKITFWP LFSKRVETK TKIKTINTA QELEDETKT 
        RPSQTLYID QQFSPNYQL INENIYRHI KKISKIIPS 
        LAQTDPPLT PISPEEIKS IISKASNKK SPGPDKIPK 
        KPLKLLPHK TIIKFTNIL NAILRLRHL PQSWKHAHI 
        ILIPKPGKD PTFPQNYRP INLLSNVSK ISERTILNR 
        LTEELNHNQ TLPNEQFGF RSHHDTTPQ LLRVTERIT 
        NNFNNKNKT AMICFDIEK AFDKVWHPG IIAKLCKAN 
        LNFRLTEPI MSFLKNRTF QVKSQNQLS NTKKCKAGV 
        PQEAILSPT LFNIFTHDI PKHTQTEIA LFADDITIM 
        TTSSK
      CDS sequence
      • >TC016413 RA
        atgcgcaaa aaggacaaa accccatta cccctcgtg 
        attgtggaa ctacctaag catgaaact aacatatac 
        aatatcact acatgtgtc agcttagac gttcgtatt 
        gaaccccta aaaaaaaca aaccaaatt ggtcaatgt 
        ttcaactgt caaagctac ggccactca caacgaaac 
        tgccatgct accgcaaaa tgtctaaag tgcgctgaa 
        acccacccc tcacaagag tgtcctaaa ccgaaaaca 
        acaacagca aaatgcacc aattataac gggccccac 
        ccggcaagc tacagaggc tgcccagta tacccaccc 
        aatcaacaa atgagagct ataaaactg acatctaac 
        ccatggaca aaaaataca acccaagtt gaccaaagt 
        tcccctccg tcagcatta aatacagaa aacttcccc 
        cctttgaaa ccaaaagcg tggaatgcc aacggccta 
        cacaacaaa ctaaacgag ctccaccaa tttacatta 
        ctcaacaat atcgacatt atgttaatt aacgagact 
        catgcaaag ccctcagac acactcaaa attccaaac 
        tacattcag tacaggaca gatcgacaa actcaccga 
        ggatgtggc acagcaata tatctgaaa caaaaccta 
        aaacaccat caaccgtcc caaataacc actgaaaat 
        atcgaaaac acgtccatc gtatttcag actaaaaac 
        ggacccctt caaattacc tctgcatac aaacctccg 
        cacaaagac ctaatcagc actgaccta cacaaactt 
        atcaaaact aaaattccc acacttata gcaggaaac 
        ctcaactgc aaacagcgt tcctggaac agtctcaac 
        aaccaaaat gaacaaaca cttctcgaa tacacatac 
        caaaacaac tgtctaatt ctgggcccc acgaaacct 
        actcacttc ggagcccac agcgctcct gacgtcctc 
        gacattgcc gtaataaaa gatatcacc cttgctatc 
        gaaatattc acaattaat gaactaagc tccgactac 
        aacccgatc attatagaa cttaacacc acaagccgt 
        ttaactccg gaaattaag aaaataaca ttttggccc 
        ttattcagt aaaagggtc gaaaccaaa acaaaaatt 
        aaaaccatt aatacagca caagaactt gaagatgaa 
        acaaaaacg aggccttcg cagacactc tatatagac 
        caacagttc tcccccaac taccaacta attaacgaa 
        aacatctac cgacacata aagaaaatt tccaaaatc 
        attccatca ctcgcgcaa accgacccc ccgctgact 
        cctatctca cctgaagaa atcaaaagc attatttcc 
        aaagccagc aacaaaaaa tcacccgga cctgacaaa 
        atacccaag aagcctctg aaactactt ccacacaaa 
        actattata aagtttacc aatattctc aacgcaatc 
        ctccgtcta agacacttg ccacaaagt tggaaacat 
        gctcacatc atactcata ccaaaacca ggcaaggac 
        ccgacattt ccacaaaac tataggcca atcaaccta 
        ttatcaaac gtaagcaaa atttcagaa cgtaccatt 
        ctcaacaga ctaacagaa gaactcaat cacaatcaa 
        actctacca aacgaacaa ttcggtttc agaagccac 
        catgacacg acaccacaa ttacttaga gtaaccgag 
        agaatcacc aacaacttc aataacaaa aataaaacc 
        gcaatgatt tgcttcgac atagagaaa gcgtttgat 
        aaagtatgg catcctggc atcatagcc aaactctgc 
        aaagccaac cttaacttt agactcact gagccaatt 
        atgtccttt ctgaagaac agaacattc caagttaaa 
        tcacaaaac cagctctct aatacaaaa aagtgcaag 
        gcgggggta ccgcaagaa gcgatactg tcacccacc 
        ctctttaac atcttcaca cacgacatt cccaaacac 
        actcagacg gaaatagcc ctattcgca gacgacata 
        acaataatg acaacatca tccaagtga
      • >TC016413 RB
        atgagagct ataaaactg acatctaac ccatggaca 
        aaaaataca acccaagtt gaccaaagt tcccctccg 
        tcagcatta aatacagaa aacttcccc cctttgaaa 
        ccaaaagcg tggaatgcc aacggccta cacaacaaa 
        ctaaacgag ctccaccaa tttacatta ctcaacaat 
        atcgacatt atgttaatt aacgagact catgcaaag 
        ccctcagac acactcaaa attccaaac tacattcag 
        tacaggaca gatcgacaa actcaccga ggatgtggc 
        acagcaata tatctgaaa caaaaccta aaacaccat 
        caaccgtcc caaataacc actgaaaat atcgaaaac 
        acgtccatc gtatttcag actaaaaac ggacccctt 
        caaattacc tctgcatac aaacctccg cacaaagac 
        ctaatcagc actgaccta cacaaactt atcaaaact 
        aaaattccc acacttata gcaggaaac ctcaactgc 
        aaacagcgt tcctggaac agtctcaac aaccaaaat 
        gaacaaaca cttctcgaa tacacatac caaaacaac 
        tgtctaatt ctgggcccc acgaaacct actcacttc 
        ggagcccac agcgctcct gacgtcctc gacattgcc 
        gtaataaaa gatatcacc cttgctatc gaaatattc 
        acaattaat gaactaagc tccgactac aacccgatc 
        attatagaa cttaacacc acaagccgt ttaactccg 
        gaaattaag aaaataaca ttttggccc ttattcagt 
        aaaagggtc gaaaccaaa acaaaaatt aaaaccatt 
        aatacagca caagaactt gaagatgaa acaaaaacg 
        aggccttcg cagacactc tatatagac caacagttc 
        tcccccaac taccaacta attaacgaa aacatctac 
        cgacacata aagaaaatt tccaaaatc attccatca 
        ctcgcgcaa accgacccc ccgctgact cctatctca 
        cctgaagaa atcaaaagc attatttcc aaagccagc 
        aacaaaaaa tcacccgga cctgacaaa atacccaag 
        aagcctctg aaactactt ccacacaaa actattata 
        aagtttacc aatattctc aacgcaatc ctccgtcta 
        agacacttg ccacaaagt tggaaacat gctcacatc 
        atactcata ccaaaacca ggcaaggac ccgacattt 
        ccacaaaac tataggcca atcaaccta ttatcaaac 
        gtaagcaaa atttcagaa cgtaccatt ctcaacaga 
        ctaacagaa gaactcaat cacaatcaa actctacca 
        aacgaacaa ttcggtttc agaagccac catgacacg 
        acaccacaa ttacttaga gtaaccgag agaatcacc 
        aacaacttc aataacaaa aataaaacc gcaatgatt 
        tgcttcgac atagagaaa gcgtttgat aaagtatgg 
        catcctggc atcatagcc aaactctgc aaagccaac 
        cttaacttt agactcact gagccaatt atgtccttt 
        ctgaagaac agaacattc caagttaaa tcacaaaac 
        cagctctct aatacaaaa aagtgcaag gcgggggta 
        ccgcaagaa gcgatactg tcacccacc ctctttaac 
        atcttcaca cacgacatt cccaaacac actcagacg 
        gaaatagcc ctattcgca gacgacata acaataatg 
        acaacatca tccaagtga
      mRNA sequence
      • >TC016413 RA
        tactccaag ataaggaca aatggccca aggtcaaaa 
        tcagcgaac taaaaatta ccattacca aggctaaaa 
        catcaaaca aaggcatac aaatttaca cgtcatcaa 
        taaatgact atagagtac tgacttaat atctaacga 
        aaaaagaat ataattacc acacttaca caatagaat 
        ccgagaaga atctaaaaa tcgttatcc gcggactcc 
        cggaatcac ttgacttaa acttaatca acgaagacc 
        ttaaaaaca aaaatttta acccaataa aaaccacgc 
        gaatgcgca aaaaggaca aaaccccat tacccctcg 
        tgattgtgg aactaccta agcatgaaa ctaacatat 
        acaatatca ctacatgtg tcagcttag acgttcgta 
        ttgaacccc taaaaaaaa caaaccaaa ttggtcaat 
        gtttcaact gtcaaagct acggccact cacaacgaa 
        actgccatg ctaccgcaa aatgtctaa agtgcgctg 
        aaacccacc cctcacaag agtgtccta aaccgaaaa 
        caacaacag caaaatgca ccaattata acgggcccc 
        acccggcaa gctacagag gctgcccag tatacccac 
        ccaatcaac aaatgagag ctataaaac tgacatcta 
        acccatgga caaaaaata caacccaag ttgaccaaa 
        gttcccctc cgtcagcat taaatacag aaaacttcc 
        cccctttga aaccaaaag cgtggaatg ccaacggcc 
        tacacaaca aactaaacg agctccacc aatttacat 
        tactcaaca atatcgaca ttatgttaa ttaacgaga 
        ctcatgcaa agccctcag acacactca aaattccaa 
        actacattc agtacagga cagatcgac aaactcacc 
        gaggatgtg gcacagcaa tatatctga aacaaaacc 
        taaaacacc atcaaccgt cccaaataa ccactgaaa 
        atatcgaaa acacgtcca tcgtatttc agactaaaa 
        acggacccc ttcaaatta cctctgcat acaaacctc 
        cgcacaaag acctaatca gcactgacc tacacaaac 
        ttatcaaaa ctaaaattc ccacactta tagcaggaa 
        acctcaact gcaaacagc gttcctgga acagtctca 
        acaaccaaa atgaacaaa cacttctcg aatacacat 
        accaaaaca actgtctaa ttctgggcc ccacgaaac 
        ctactcact tcggagccc acagcgctc ctgacgtcc 
        tcgacattg ccgtaataa aagatatca cccttgcta 
        tcgaaatat tcacaatta atgaactaa gctccgact 
        acaacccga tcattatag aacttaaca ccacaagcc 
        gtttaactc cggaaatta agaaaataa cattttggc 
        ccttattca gtaaaaggg tcgaaacca aaacaaaaa 
        ttaaaacca ttaatacag cacaagaac ttgaagatg 
        aaacaaaaa cgaggcctt cgcagacac tctatatag 
        accaacagt tctccccca actaccaac taattaacg 
        aaaacatct accgacaca taaagaaaa tttccaaaa 
        tcattccat cactcgcgc aaaccgacc ccccgctga 
        ctcctatct cacctgaag aaatcaaaa gcattattt 
        ccaaagcca gcaacaaaa aatcacccg gacctgaca 
        aaataccca agaagcctc tgaaactac ttccacaca 
        aaactatta taaagttta ccaatattc tcaacgcaa 
        tcctccgtc taagacact tgccacaaa gttggaaac 
        atgctcaca tcatactca taccaaaac caggcaagg 
        acccgacat ttccacaaa actataggc caatcaacc 
        tattatcaa acgtaagca aaatttcag aacgtacca 
        ttctcaaca gactaacag aagaactca atcacaatc 
        aaactctac caaacgaac aattcggtt tcagaagcc 
        accatgaca cgacaccac aattactta gagtaaccg 
        agagaatca ccaacaact tcaataaca aaaataaaa 
        ccgcaatga tttgcttcg acatagaga aagcgtttg 
        ataaagtat ggcatcctg gcatcatag ccaaactct 
        gcaaagcca accttaact ttagactca ctgagccaa 
        ttatgtcct ttctgaaga acagaacat tccaagtta 
        aatcacaaa accagctct ctaatacaa aaaagtgca 
        aggcggggg taccgcaag aagcgatac tgtcaccca 
        ccctcttta acatcttca cacacgaca ttcccaaac 
        acactcaga cggaaatag ccctattcg cagacgaca 
        taacaataa tgacaacat catccaagt gacctaaac 
        tagcaacca aacgcctcc aagaacaca ctgaccaac 
        taatcaatt tctacaaca aatggggca ttcaaataa 
        accccgaca aaacgcaag caatactct tttcaaaat 
        ttcacgcca ccccacaac aaaatgtca aaatcaaca 
        atgtagtca tcgaatggg aaaaacata ttaaatctt 
        aggcctgac cttagacac tggccttac ctacaaacc 
        gcacctaaa gaatatagc caacaaaac caaaagagc 
        cctaagcag tctataccc cttactaaa tcgaaacag 
        ccaactgaa tctatctaa taaaagact tctctacct
      • >TC016413 RB
        gcgcaacat actaaacta taataacac tgcagataa 
        ggacaaatg gcccaaggt caaaatcag cgaactaaa 
        aattaccat taccaaggc taaaacatc aaacaaagg 
        catacaaat ttacacgtc atcaataaa tgactatag 
        agtactgac ttaatatct aacgaaaaa agaatataa 
        ttaccacac ttacacaat agaatccga gaagaatct 
        aaaaatcgt tatccgcgg actcccgga atcacttga 
        cttaaactt aatcaacga agaccttaa aaacaaaaa 
        ttttaaccc aataaaaac cacgcgaat gcgcaaaaa 
        ggacaaaac cccattacc cctcgtgat tgtggaact 
        acctaagca tgaaactaa catatacaa tatcactac 
        atgtgtcag cttagacgt tcgtattga acccctaaa 
        aaaaacaaa ccaaattgg tcaatgttt caactgtca 
        aagctacgg ccactcaca acgaaactg ccatgctac 
        cgcaaaatg tctaaagtg cgctgaaac ccacccctc 
        acaagagtg tcctaaacc gaaaacaac aacagcaaa 
        atgcaccaa ttataacgg gccccaccc ggcaagcta 
        cagaggctg cccagtata cccacccaa tcaacaaat 
        gagagctat aaaactgac atctaaccc atggacaaa 
        aaatacaac ccaagttga ccaaagttc ccctccgtc 
        agcattaaa tacagaaaa cttcccccc tttgaaacc 
        aaaagcgtg gaatgccaa cggcctaca caacaaact 
        aaacgagct ccaccaatt tacattact caacaatat 
        cgacattat gttaattaa cgagactca tgcaaagcc 
        ctcagacac actcaaaat tccaaacta cattcagta 
        caggacaga tcgacaaac tcaccgagg atgtggcac 
        agcaatata tctgaaaca aaacctaaa acaccatca 
        accgtccca aataaccac tgaaaatat cgaaaacac 
        gtccatcgt atttcagac taaaaacgg accccttca 
        aattacctc tgcatacaa acctccgca caaagacct 
        aatcagcac tgacctaca caaacttat caaaactaa 
        aattcccac acttatagc aggaaacct caactgcaa 
        acagcgttc ctggaacag tctcaacaa ccaaaatga 
        acaaacact tctcgaata cacatacca aaacaactg 
        tctaattct gggccccac gaaacctac tcacttcgg 
        agcccacag cgctcctga cgtcctcga cattgccgt 
        aataaaaga tatcaccct tgctatcga aatattcac 
        aattaatga actaagctc cgactacaa cccgatcat 
        tatagaact taacaccac aagccgttt aactccgga 
        aattaagaa aataacatt ttggccctt attcagtaa 
        aagggtcga aaccaaaac aaaaattaa aaccattaa 
        tacagcaca agaacttga agatgaaac aaaaacgag 
        gccttcgca gacactcta tatagacca acagttctc 
        ccccaacta ccaactaat taacgaaaa catctaccg 
        acacataaa gaaaatttc caaaatcat tccatcact 
        cgcgcaaac cgacccccc gctgactcc tatctcacc 
        tgaagaaat caaaagcat tatttccaa agccagcaa 
        caaaaaatc acccggacc tgacaaaat acccaagaa 
        gcctctgaa actacttcc acacaaaac tattataaa 
        gtttaccaa tattctcaa cgcaatcct ccgtctaag 
        acacttgcc acaaagttg gaaacatgc tcacatcat 
        actcatacc aaaaccagg caaggaccc gacatttcc 
        acaaaacta taggccaat caacctatt atcaaacgt 
        aagcaaaat ttcagaacg taccattct caacagact 
        aacagaaga actcaatca caatcaaac tctaccaaa 
        cgaacaatt cggtttcag aagccacca tgacacgac 
        accacaatt acttagagt aaccgagag aatcaccaa 
        caacttcaa taacaaaaa taaaaccgc aatgatttg 
        cttcgacat agagaaagc gtttgataa agtatggca 
        tcctggcat catagccaa actctgcaa agccaacct 
        taactttag actcactga gccaattat gtcctttct 
        gaagaacag aacattcca agttaaatc acaaaacca 
        gctctctaa tacaaaaaa gtgcaaggc gggggtacc 
        gcaagaagc gatactgtc acccaccct ctttaacat 
        cttcacaca cgacattcc caaacacac tcagacgga 
        aatagccct attcgcaga cgacataac aataatgac 
        aacatcatc caagtgacc taaactagc aaccaaacg 
        cctccaaga acacactga ccaactaat caatttcta 
        caacaaatg gggcattca aataaaccc cgacaaaac 
        gcaagcaat actcttttc aaaatttca cgccacccc 
        acaacaaaa tgtcaaaat caacaatgt agtcatcga 
        atgggaaaa acatattaa atcttaggc ctgacctta 
        gacactggc cttacctac aaaccgcac ctaaagaat 
        atagccaac aaaaccaaa agagcccta agcagtcta 
        tacccctta ctaaatcga aacagccaa ctgaatcta 
        tctaataaa agacttctc tacct
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY